scholarly journals INFLUENCE OF DIETARY PROTEIN CONTENT ON Trypanosoma cruzi INFECTION IN GERMFREE AND CONVENTIONAL MICE

1998 ◽  
Vol 40 (6) ◽  
pp. 355-362 ◽  
Author(s):  
Isa P. CINTRA ◽  
Marcelo E. SILVA ◽  
Marcílio E.C. SILVA ◽  
Márcio E. SILVA ◽  
L.C. C. AFONSO ◽  
...  
Keyword(s):  
Weight Gain ◽  
Trypanosoma Cruzi ◽  
Dietary Protein ◽  
Blood Cell Count ◽  
Deficient Diet ◽  
Low Protein ◽  
Protein Deficient Diet ◽  
Red Blood Cell Count ◽  
Cruzi Infection ◽  
Microbiological Status

Germfree (GF) and conventional (CV) mice were fed on diets containing 4.4, 13.2 or 26.4% of protein (weight/weight). CV mice fed on low protein diet did not gain weight during four weeks, whereas the protein deficient diet did not affect the growth of GF mice. After four weeks on these diets, the mice were inoculated with 5x103 trypomastigotes of Trypanosoma cruzi. The protein deficiency affected less the GF than the CV mice, according to the following parameters: weight gain, hemoglobin, plasma protein and albumin levels and water and protein contents of the carcass. Infection with T. cruzi produced a significant decrease in hemoglobin levels, red blood cell count, and water and protein contents in the carcass. This decrease was more pronounced in the GF mice. Histopathologically, there was no difference between the treatments in animals with the same microbiological status (GF or CV). However, the disease was more severe in the GF than in the CV mice.

Emergence of a Kwashiorkor-Like Syndrome Associated with Protein-Calorie Malnutrition in the Developing Rhesus Monkey (Macaca Mulatta)

1977 ◽  
Vol 40 (3_suppl) ◽  
pp. 1339-1344
Author(s):  
Charles R. Geist ◽  
Olin W. Smith ◽  
Robert R. Zimmermann ◽  
Enid M. Geist
Keyword(s):  
Rhesus Monkey ◽  
Terminal State ◽  
Deficient Diet ◽  
Dietary Shift ◽  
Protein Calorie Malnutrition ◽  
Low Protein ◽  
The Face ◽  
Protein Deficient Diet ◽  
Dietary Regime ◽  
Sparse Hair

An infant rhesus monkey was separated from its mother at 90 days of age, housed individually, and at 120 days of age placed on a purified low protein diet containing 3.5% casein by weight. To enhance the effects of protein-calorie malnutrition, a protein-deficient diet containing 2.0% casein by weight was introduced at 415 days of age. When compared with other animals on an identical dietary regime, the subject manifested a reduced intake of food and a marked decrease in weight gain. Clinical symptomatology analogous to human kwashiorkor progressively developed into an acute phase as a result of protein deficiency and included: brittle, depigmented, and sparse hair; chronic diarrhea; dermatitis as “flaky-paint rash” on the extremities and “moist groin rash;” edema of the face and extremities; hypoalbuminemia; hypoproteinemia; retardation of growth and physical development; and wasting of muscle tissue. Within 172 days following the dietary shift from 3.5% to 2.0% casein, a terminal state was evidenced.

scholarly journals IP3/Ca2+ signals regulate larval to pupal transition under nutrient stress through the H3K36 methyltransferase dSET2

2020 ◽  
Author(s):  
Rishav Mitra ◽  
Shlesha Richhariya ◽  
Siddharth Jayakumar ◽  
Dimple Notani ◽  
Gaiti Hasan
Keyword(s):  
Dietary Protein ◽  
Feedback Loop ◽  
Deficient Diet ◽  
Release Channel ◽  
Regulate Gene Expression ◽  
Critical Weight ◽  
Histone 3 ◽  
Intracellular Ca ◽  
Protein Deficient Diet ◽  
Neuronal Genes

AbstractPersistent loss of dietary protein usually signals a shutdown of key metabolic pathways. In Drosophila larvae, that have crossed “critical weight” and can pupariate to form viable adults, such a metabolic shut-down would needlessly lead to death. IP3/Ca2+ signals in certain interneurons (vGlutVGN6341) allow Drosophila larvae to pupariate on a protein-deficient diet by partially circumventing this shutdown through upregulation of neuropeptide signaling and the expression of ecdysone synthesis genes. Here we show that IP3/Ca2+ signals in vGlutVGN6341 neurons drive expression of dSET2, a Drosophila Histone 3 Lysine 36 methyltransferase. Further, dSET2 expression is required for larvae to pupariate in the absence of dietary protein. IP3/Ca2+ signal-driven dSET2 expression upregulates key Ca2+ signaling genes through a novel positive feedback loop. Transcriptomic studies coupled with analysis of existing ChIP-seq datasets identified genes from larval and pupal stages, that normally exhibit robust H3K36 trimethyl marks on their gene bodies and concomitantly undergo stronger downregulation by knockdown of either an intracellular Ca2+ release channel the IP3R or dSET2. IP3/Ca2+ signals thus regulate gene expression through dSET2 mediated H3K36 marks on select neuronal genes for the larval to pupal transition.

Corrigendum to: IGF-I is not a useful marker for nutritional status in the growing pig under commercial conditions

2001 ◽  
Vol 52 (7) ◽  
pp. 791
Author(s):  
L. Ma ◽  
F. R. Dunshea ◽  
Y. M. Brockwell ◽  
R. L. Inglis ◽  
D. J. Kingston ◽  
...  
Keyword(s):  
Weight Gain ◽  
Nutritional Status ◽  
Dietary Protein ◽  
High Protein Diet ◽  
High Protein ◽  
Protein Diet ◽  
Low Protein ◽  
Igf I ◽  
Protein Diets

Plasma hormone concentrations were measured in gilts after fasting, long-term protein restriction, or supplementation. In 11-week-old pigs fasted overnight, plasma insulin, glucagon, gastrin, urea, and glucose were increased 30 min after re-feeding (P < 0.05), whereas IGF-I did not change. In 16-week-old gilts fed a standard commercial diet [14.6% crude protein (CP)], or a high-protein diet (16.7% CP) for 4 weeks, the high-protein diet increased weight gain (13%; P < 0.05) and carcass weight (4%; P < 0.05), but did not alter plasma IGF-I, insulin, or glucagon. In 10-week-old gilts fed high-protein diets (19.4% and 18.3% CP), or low-protein diets (15.5% and 13.3% CP) for 12 weeks during the grower and finisher phases, respectively, the low-protein diet decreased weight gain (18%; P < 0.001) and carcass weight (11%; P < 0.01), with a marked increase in plasma glucagon (P < 0.05), no change in insulin, and only a trend towards decreased IGF-I (P = 0.1). The pigs were more sensitive to altered dietary protein at 10 weeks of age than at 16 weeks. Plasma IGF-I was not responsive to the short-term effects of feeding or the long-term effects of dietary protein. Glucagon could provide a useful marker for nutritional status in young pigs, provided that time of feeding is taken into account.

scholarly journals The effects of energy intake and dietary protein on nitrogen retention, growth performance, body composition and some aspects of energy metabolism of baby pigs

1983 ◽  
Vol 49 (2) ◽  
pp. 221-230 ◽  
Author(s):  
R. G. Campbell ◽  
A. C. Dunkin
Keyword(s):  
Body Composition ◽  
Growth Performance ◽  
Energy Intake ◽  
Body Fat ◽  
Dietary Protein ◽  
Nitrogen Retention ◽  
Energy Utilization ◽  
Deficient Diet ◽  
Adequate Diet ◽  
Protein Deficient Diet

1. The effects of level of feeding of either a protein-adequate or a protein-deficient diet on nitrogen retention (NR), growth performance, body composition and some aspects of energy utilization in pigs growing from 1·8 to 6·5 kg live weight (LW) were investigated in two experiments.2. In Expts 1 and 2 piglets were given a protein-adequate diet at four levels of intake (0·93, 1·44, 1·83 and 2·30 MJ gross energy (GE)/kg LW0·75 per d) and a protein-deficient diet at five levels of intake (1·14, 1·38, 1·68, 1·95 and 2·30 MJ GE/kg LW0·75 per d) respectively.3. For pigs given the protein-adequate diet (Expt 1) NR was linearly (P < 0·001) related to energy intake (EI) and independent of N intake (NI). NR in pigs given the protein-deficient diet (Expt 2) was linearly (P < 0·001) related to NI and independent of EI.4. Average daily LW gain responded linearly to increases in EI in both experiments. However, at equivalent levels of EI pigs given the protein-adequate diet exhibited more rapid and efficient growth than those given the protein-deficient diet. The results also indicated an interaction between the effects of EI and dietary protein content for feed conversion efficiency.5. Body fat at 6·5 kg LW increased in a curvilinear fashion with increasing EI in both experiments. However, over the range of EI tested (from approximately 1·8 to 4·6 times energy for maintenance) body fat increased by 153% in Expt 1 and by only 27% in Expt 2. Pigs given the protein-deficient diet were also markedly fatter than those given the protein-adequate diet. Body protein at 6·5 kg LW decreased (P < 0·01) with increasing EI in Expt 1 but was unaffected by EI in Expt 2.6. As estimated by multiple regression analysis, the values for the efficiency of energy utilization for protein (kp) and fat (kf) deposition were 0·76 and 0·78 respectively in Expt 1 and 0·42 and 0·89 resepctively in Expt 2. The estimates of metabolizable energy required for maintenance were 445 and 532 kJ/kg LW0·75 per d for Expts 1 and 2 respectively.

scholarly journals Dietary protein and the growth of rats infected with the tapeworm Hymenolepis diminuta

1983 ◽  
Vol 49 (1) ◽  
pp. 59-65 ◽  
Author(s):  
Andrew Hall
Keyword(s):  
Weight Gain ◽  
Food Intake ◽  
Dietary Protein ◽  
High Protein Diet ◽  
Hymenolepis Diminuta ◽  
Protein Diet ◽  
Daily Food Intake ◽  
Low Protein ◽  
Daily Food ◽  
Weanling Rats

1. Weanling rats fed on a relatively low protein diet were infected with several intestinal tapeworms. The weight gain and daily food intake of the rats were measured for 3 weeks before the animals were killed and the adult tapeworms recovered. The tissues of the rats and worms were then analysed for protein. Controls were provided by uninfected rats either pair fed or fed ad lib. For comparison, a similar experiment was conducted on rats fed on a relatively high protein diet.2. The effect of the infection on the protein-malnourished rats and of the low level of protein on the worms were apparently not significant. The amount of protein contained in the worm burdens was less than 1·5% of the average total intake of the protein-malnourished rats.

Folic Acid Anemia in Coho Salmon

1969 ◽  
Vol 26 (1) ◽  
pp. 111-114 ◽  
Author(s):  
Charlie E. Smith ◽  
John E. Halver
Keyword(s):  
Folic Acid ◽  
Coho Salmon ◽  
Macrocytic Anemia ◽  
Folic Acid Deficiency ◽  
Blood Cell Count ◽  
Deficient Diet ◽  
Blood Smears ◽  
Acid Deficiency ◽  
Red Blood Cell Count ◽  
Peripheral Blood Smears

Juvenile coho salmon (Oncorhynchus kisutch) fed a folic acid-deficient diet and sampled at 6, 9, 12, and 14 weeks developed macrocytic anemia. The anemia, first observed at 6 weeks, was characterized by a significant reduction in red blood cell count as well as macrocytosis and poikilocytosis of erythrocytes. The abnormally shaped erythrocytes observed in peripheral blood smears may be an important aid in the identification of folic acid deficiency in coho salmon. Gross manifestations of the deficiency were extremely pale gills; exophthalmia, often accompanied by ascites fluid; dark coloration; and reduction in growth. Fish recovered after 8 weeks on a diet adequate in folic acid and exhibited a normal blood picture.

scholarly journals IP3-mediated Ca2+ signals regulate larval to pupal transition under nutrient stress through the H3K36 methyltransferase Set2

Development ◽  
2021 ◽  
Vol 148 (11) ◽  
Author(s):  
Rishav Mitra ◽  
Shlesha Richhariya ◽  
Siddharth Jayakumar ◽  
Dimple Notani ◽  
Gaiti Hasan
Keyword(s):  
Dietary Protein ◽  
Gene Encoding ◽  
Deficient Diet ◽  
Release Channel ◽  
Regulate Gene Expression ◽  
Critical Weight ◽  
Histone 3 ◽  
Drosophila Larvae ◽  
Protein Deficient Diet ◽  
Neuronal Genes

ABSTRACT Persistent loss of dietary protein usually signals a shutdown of key metabolic pathways. In Drosophila larvae that have reached a ‘critical weight’ and can pupariate to form viable adults, such a metabolic shutdown would needlessly lead to death. Inositol 1,4,5-trisphosphate-mediated calcium (IP3/Ca2+) release in some interneurons (vGlutVGN6341) allows Drosophila larvae to pupariate on a protein-deficient diet by partially circumventing this shutdown through upregulation of neuropeptide signaling and the expression of ecdysone synthesis genes. Here, we show that IP3/Ca2+ signals in vGlutVGN6341 neurons drive expression of Set2, a gene encoding Drosophila Histone 3 Lysine 36 methyltransferase. Furthermore, Set2 expression is required for larvae to pupariate in the absence of dietary protein. IP3/Ca2+ signal-driven Set2 expression upregulates key Ca2+-signaling genes through a novel positive-feedback loop. Transcriptomic studies, coupled with analysis of existing ChIP-seq datasets, identified genes from larval and pupal stages that normally exhibit robust H3K36 trimethyl marks on their gene bodies and concomitantly undergo stronger downregulation by knockdown of either the intracellular Ca2+ release channel IP3R or Set2. IP3/Ca2+ signals thus regulate gene expression through Set2-mediated H3K36 marks on select neuronal genes for the larval to pupal transition.

IGF-I is not a useful marker for nutritional status in the growing pig under commercial conditions

2001 ◽  
Vol 52 (5) ◽  
pp. 603
Author(s):  
L. Ma ◽  
F. R. Dunshea ◽  
Y. M. Brockwell ◽  
R. L. Inglis ◽  
D. J. Kingston ◽  
...  
Keyword(s):  
Weight Gain ◽  
Nutritional Status ◽  
Dietary Protein ◽  
High Protein Diet ◽  
High Protein ◽  
Protein Diet ◽  
Low Protein ◽  
Igf I ◽  
Protein Diets

Plasma hormone concentrations were measured in gilts after fasting, long-term protein restriction, or supplementation. In 11-week-old pigs fasted overnight, plasma insulin, glucagon, gastrin, urea, and glucose were increased 30 min after re-feeding (P < 0.05), whereas IGF-I did not change. In 16-week-old gilts fed a standard commercial diet [14.6% crude protein (CP)], or a high-protein diet (16.7% CP) for 4 weeks, the high-protein diet increased weight gain (13%; P < 0.05) and carcass weight (4%; P < 0.05), but did not alter plasma IGF-I, insulin, or glucagon. In 10-week-old gilts fed high-protein diets (19.4% and 18.3% CP), or low-protein diets (15.5% and 13.3% CP) for 12 weeks during the grower and finisher phases, respectively, the low-protein diet decreased weight gain (18%; P < 0.001) and carcass weight (11%; P < 0.01), with a marked increase in plasma glucagon (P < 0.05), no change in insulin, and only a trend towards decreased IGF-I (P = 0.1). The pigs were more sensitive to altered dietary protein at 10 weeks of age than at 16 weeks. Plasma IGF-I was not responsive to the short-term effects of feeding or the long-term effects of dietary protein. Glucagon could provide a useful marker for nutritional status in young pigs, provided that time of feeding is taken into account.

Dietary protein and zinc restrictions independently modify a Heligmosomoides polygyrus (Nematoda) infection in mice

Parasitology ◽  
1998 ◽  
Vol 116 (5) ◽  
pp. 449-462 ◽  
Author(s):  
M. BOULAY ◽  
M. E. SCOTT ◽  
S. L. CONLY ◽  
M. M. STEVENSON ◽  
K. G. KOSKI
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Dietary Protein ◽  
Body Weight Gain ◽  
Challenge Infection ◽  
Host Susceptibility ◽  
Albumin Concentration ◽  
Heligmosomoides Polygyrus ◽  
Low Protein ◽  
Marginal Zinc Deficiency

The effects of dietary protein and zinc restrictions on Heligmosomoides polygyrus were compared following primary and challenge infection in female BALB/c mice fed either control (24%), marginal (7%) or low (3%) protein combined with either high or low zinc (60 or 3 mg Zn/kg diet). Dietary protein restriction (3%) resulted in significantly lower body weight gain. As well, blood urea nitrogen (BUN) significantly decreased with decreasing dietary protein level. However, neither plasma albumin concentration nor relative thymus or spleen weights were reduced. Marginal zinc deficiency was confirmed by significantly lower tibia and liver zinc concentration, but food intake, body weight gain, relative thymus and spleen weights, and alkaline phosphatase activity were not altered. On day 29 post-primary infection, worm burdens were significantly higher in mice fed either marginal or low protein and in mice fed a low zinc diet, while parasite egg output was significantly higher in mice fed both low protein and low zinc diets. Immune status was compromised in mice fed low protein (significantly lower serum IgG1 and lower eosinophilia), and in mice fed low zinc diet (significantly lower eosinophilia). Early in the infection, IgE titres were elevated in mice fed low protein or low zinc, but IgE titres declined to levels lower than the control diet groups after 14–21 days. On day 29 post-challenge infection, worm burdens and parasite egg output were significantly higher in mice fed low protein, whereas the other groups had expelled almost all parasites. Dietary restriction had no effect on serum IgE. Significantly reduced serum IgG1 titres and eosinophilia in mice fed 3% protein supported the view that low dietary protein but not low zinc increased host susceptibility to H. polygyrus by compromising host immune function following reinfection in immunized mice.

Reactions to Intrinsic and Extrinsic Rewards in Protein-Malnourished Monkeys

1973 ◽  
Vol 36 (3) ◽  
pp. 787-790 ◽  
Author(s):  
Beatrice Aakre ◽  
David A. Strobel ◽  
R. R. Zimmermann ◽  
C. R. Geist
Keyword(s):  
High Protein Diet ◽  
Partial Reinforcement ◽  
High Protein ◽  
Extrinsic Reward ◽  
Deficient Diet ◽  
Rapid Reduction ◽  
Intrinsic Reward ◽  
Low Protein ◽  
Significant Difference ◽  
Protein Deficient Diet

10 rhesus monkeys were tested on a 12-part puzzle manipulation apparatus. 6 of 10 Ss were maintained on a protein-deficient diet containing 3.5% casein by weight, while the remaining 4 Ss received a high-protein diet containing 25% casein by weight. The high-protein animals had an elevated manipulation rate as compared with low-protein fed Ss when the only source of reinforcement was the manipulation of the puzzles (intrinsic reward). However, when food (extrinsic reward) was introduced the low-protein fed Ss manipulated at a level equal to or greater than the high-protein fed animals. Introduction of 100% and partial reinforcement conditions showed manipulation rates to be relatively consistent between the two groups. Extinction conditions, however, showed a significant difference between the high- and low-protein fed animals after partial reinforcement. With the removal of the food reward the low-protein fed animals exhibited a much more rapid reduction of manipulatory activity than the high-protein fed Ss. The more rapid extinction of the manipulation response by low-protein fed monkeys suggests that extrinsic reward is a much more salient variable for these Ss than intrinsic reward.

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