scholarly journals Comparison of bacterial diversity and abundance between sexes of Leptocybe invasa Fisher & La Salle (Hymenoptera: Eulophidae) from China

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8411
Author(s):  
Chunhui Guo ◽  
Xin Peng ◽  
Xialin Zheng ◽  
Xiaoyun Wang ◽  
Ruirui Wang ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Bacterial Diversity ◽  
Bacterial Communities ◽  
Small Body ◽  
Bacterial Community Composition ◽  
Illumina Miseq ◽  
Invasive Pest ◽  
Comprehensive Comparison ◽  
Leptocybe Invasa ◽  
Dominant Bacteria

Background Insects harbor a myriad of microorganisms, many of which can affect the sex ratio and manipulate the reproduction of the host. Leptocybe invasa is an invasive pest that causes serious damage to eucalyptus plantations, and the thelytokous parthenogenesis, low temperature resistance, protection in galls, generation overlap and small body of L. invasa contribute to its rapid invasion and population growth. However, the endosymbiotic bacterial composition, abundance and sex differences of L. invasa remain unclear. Therefore, this research aimed to identify the bacterial communities in L. invasa adults and compare them between the sexes of L. invasa lineage B. Results The Illumina MiSeq platform was used to compare bacterial community composition between females and males of L. invasa by sequencing the V3–V4 region of the 16S ribosomal RNA gene. A total of 1,320 operational taxonomic units (OTUs) were obtained. These OTUs were subdivided into 24 phyla, 71 classes, 130 orders, 245 families and 501 genera. At the genus level, the dominant bacteria in females and males were Rickettsia and Rhizobium, respectively. Conclusion The endosymbiotic bacteria of L. invasa females and males were highly diverse. There were differences in the bacterial community of L. invasa between sexes, and the bacterial diversity in male specimens was greater than that in female specimens. This study presents a comprehensive comparison of bacterial communities in L. invasa and these data will provide an overall view of the bacterial community in both sexes of L. invasa with special attention on sex-related bacteria.

scholarly journals Comparison of the bacterial abundance and diversity in the Leptocybe invasa Fisher & La Salle (Hymenoptera: Eulophidae) between both sexes

2019 ◽  
Author(s):  
Chunhui Guo ◽  
Xin Peng ◽  
Xialin Zheng ◽  
Xiaoyun Wang ◽  
Ruirui Wang ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Illumina Miseq ◽  
Invasive Pest ◽  
Operational Taxonomic Units ◽  
Adult Females ◽  
Comprehensive Comparison ◽  
Leptocybe Invasa ◽  
Sex Regulation ◽  
Abundance And Diversity ◽  
Dominant Bacteria

Background. Insects harbor a myriad of microorganisms, many of which can affect the sex ratio and manipulate the reproduction of the host. Leptocybe invasa is an invasive pest that causes serious damage to eucalyptus plantations, and both female-biased sex ratios and thelytokous parthenogenesis in L. invasa contribute to the rapid invasion and fast growth of the population. However, the interior bacterial composition and abundance of L. invasa and the differences between both sexes remain unclear. Results. The Illumina MiSeq platform was used to compare the composition of the bacterial community in adult females and males by sequencing with variation in the V3-V4 region of the 16S ribosomal DNA gene. The results showed that 1320 operational taxonomic units (OTUs) were obtained in total. These OTUs were annotated into 24 phyla, 71 classes, 130 orders, 245 families and 501 genera. At the genus level, the dominant bacteria in females and males was Rickettsia and Rhizobium, respectively. Conclusion. The bacteria living in L. invasa adult females and males had high diversity. There were differences in the bacterial community in L. invasa between both sexes, and the bacterial diversity in male adults was more abundant than that in female adults. This study presents a comprehensive comparison of bacterial communities living in L. invasa between sexes, which plays a significant role in reproductive strategy, sex regulation and the invasive mechanism of L. invasa and provides a basis for follow-up studies on the coevolution and interaction between L. invasa and its predominant bacteria.

scholarly journals Comparison of the bacterial abundance and diversity in the Leptocybe invasa Fisher & La Salle (Hymenoptera: Eulophidae) between both sexes

2019 ◽  
Author(s):  
Chunhui Guo ◽  
Xin Peng ◽  
Xialin Zheng ◽  
Xiaoyun Wang ◽  
Ruirui Wang ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Illumina Miseq ◽  
Invasive Pest ◽  
Operational Taxonomic Units ◽  
Adult Females ◽  
Comprehensive Comparison ◽  
Leptocybe Invasa ◽  
Sex Regulation ◽  
Abundance And Diversity ◽  
Dominant Bacteria

Background. Insects harbor a myriad of microorganisms, many of which can affect the sex ratio and manipulate the reproduction of the host. Leptocybe invasa is an invasive pest that causes serious damage to eucalyptus plantations, and both female-biased sex ratios and thelytokous parthenogenesis in L. invasa contribute to the rapid invasion and fast growth of the population. However, the interior bacterial composition and abundance of L. invasa and the differences between both sexes remain unclear. Results. The Illumina MiSeq platform was used to compare the composition of the bacterial community in adult females and males by sequencing with variation in the V3-V4 region of the 16S ribosomal DNA gene. The results showed that 1320 operational taxonomic units (OTUs) were obtained in total. These OTUs were annotated into 24 phyla, 71 classes, 130 orders, 245 families and 501 genera. At the genus level, the dominant bacteria in females and males was Rickettsia and Rhizobium, respectively. Conclusion. The bacteria living in L. invasa adult females and males had high diversity. There were differences in the bacterial community in L. invasa between both sexes, and the bacterial diversity in male adults was more abundant than that in female adults. This study presents a comprehensive comparison of bacterial communities living in L. invasa between sexes, which plays a significant role in reproductive strategy, sex regulation and the invasive mechanism of L. invasa and provides a basis for follow-up studies on the coevolution and interaction between L. invasa and its predominant bacteria.

scholarly journals Analysis of rhizosphere bacterial diversity of Angelica dahurica var. formosana based on different experimental sites and varieties (strains)

2021 ◽  
Author(s):  
Meiyan Jiang ◽  
Fei Yao ◽  
Yunshu Yang ◽  
Yang Zhou ◽  
Kai Hou ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Bacterial Diversity ◽  
Growth Promotion ◽  
Bacterial Community Composition ◽  
Illumina Miseq ◽  
Fertilizer Application ◽  
Angelica Dahurica ◽  
Soil Environment ◽  
Yield And Quality

Only with good producing areas and good germplasm can good medicinal materials be produced. In this study, in order to explore the effects of soil environment and germplasm on yield and quality of Angelica dahurica var. formosana from microorganism level, Illumina MiSeq was used to study the rhizosphere bacterial diversity of A. dahurica var. formosana based on different origin and varieties (strains). The results showed that the bacterial community of A. dahurica var. formosana was stable and conserved to a certain extent, and the bacteria of Proteobacteria and Firmicutes might play an important role in improving the yield and quality of A. dahurica var. formosana. Soil variables were the key factors affecting the rhizosphere bacterial community composition of A. dahurica var. formosana. These results are of great significance for further understanding the growth promotion mechanism of beneficial rhizosphere bacteria, reducing fertilizer application, expanding planting area, improving soil environment, and improving yield and quality.

scholarly journals Perennials but not slope aspect affect the diversity of soil bacterial communities in the northern Negev Desert, Israel

Soil Research ◽  
2018 ◽  
Vol 56 (2) ◽  
pp. 123 ◽  
Author(s):  
Ahuva Vonshak ◽  
Menachem Y. Sklarz ◽  
Ann M. Hirsch ◽  
Osnat Gillor
Keyword(s):  
Bacterial Community ◽  
Bacterial Diversity ◽  
Community Composition ◽  
Bacterial Communities ◽  
Bacterial Community Composition ◽  
Plant Litter ◽  
Negev Desert ◽  
Slope Aspect ◽  
Desert Shrubs ◽  
Soil Bacterial

Underneath the canopy of perennials in arid regions, moderate soil temperature and evaporation, as well as plant litter create islands of higher fertility in the low-productivity landscape, known as ‘resource islands’. The sparse distribution of these resource islands is mirrored by soil microbial communities, which mediate a large number of biogeochemical transformations underneath the plants. We explored the link between the bacterial community composition and two prevalent desert shrubs, Zygophyllum dumosum and Artemisia herba-alba, on northern- and southern-facing slopes in the northern highlands of the Negev Desert (Israel), at the end of a drought winter mild rainy season. We sequenced the bacterial community and analysed the physicochemical properties of the soil under the shrub canopies and from barren soil in replicate slopes. The soil bacterial diversity was independent of slope aspect, but differed according to shrub presence or type. Links between soil bacterial community composition and their associated desert shrubs were found, enabling us to link bacterial diversity with shrub type or barren soils. Our results suggest that plants and their associated bacterial communities are connected to survival and persistence under the harsh desert conditions.

scholarly journals Effects of different vegetable rotations on the rhizosphere bacterial community and tomato growth in a continuous tomato cropping substrate

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0257432
Author(s):  
Li Jin ◽  
Jian Lyu ◽  
Ning Jin ◽  
Jianming Xie ◽  
Yue Wu ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Diversity ◽  
Crop Rotation ◽  
Bacterial Communities ◽  
High Throughput Sequencing ◽  
Total N ◽  
Rhizosphere Bacterial Community ◽  
Healthy Growth ◽  
Dominant Bacteria

In this study, High throughput sequencing was used to analyze the effects of different vegetable rotations on the rhizosphere bacterial diversity and community structure in a substrate that was used for continuous tomato cropping (CK). The vegetable rotations tested were cabbage/tomato (B), kidney bean/tomato (D), and celery/tomato (Q). The results revealed that the substrate bacterial diversity and richness of each crop rotation were higher than those of CK. The highest bacterial diversity was found in the B substrate, followed by the Q and D substrates. Further comparison showed that the rhizosphere bacterial community structure of Q substrate was significantly different to that of CK. Compared with the CK, the Q substrate had a significantly higher relative abundance of several dominant microflora, such as Acidobacteria, Chloroflexi, and Firmicutes. Additionally, the Q rotation significantly increased the abundance of beneficial bacteria, such as Actinobacteria_unclassified and Anaerolineaceae_unclassified. A redundancy analysis showed that Most dominant bacteria correlated positively with the substrate pH, total N, and alkali-hydrolyzable N but negatively with the available P, available K, total P, total K, and organic matter contents and substrate EC. The substrates after crop rotation improved the growth and physiological condition of the subsequent tomato plants, among which those from the Q rotation performed the best. Therefore, celery rotation not only increased the richness and diversity of bacterial communities in the substrate but also significantly increased the richness of the beneficial bacterial communities, allowing better maintenance of the substrate microenvironment for the healthy growth of crops.

scholarly journals Environmental characteristics and changes of bacterial communities in the meltwater runoff of glacier in Ny-Alesund, Arctic

2021 ◽  
Author(s):  
Lidong Lin ◽  
Nengfei Wang ◽  
Wenbing Han ◽  
Botao Zhang ◽  
Jiaye Zang ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Bacterial Diversity ◽  
Bacterial Communities ◽  
High Throughput Sequencing ◽  
Bacterial Community Composition ◽  
Operational Taxonomic Units ◽  
The Core ◽  
Meltwater Runoff ◽  
Glacial Runoff ◽  
Weighted Correlation

Abstract The present study assessed the diversity and composition of bacterial communities in glacial runoff and glacial soils in the Midre Lovénbreen glacier region of Svalbard. A total of 6,593 operational taxonomic units were identified by high-throughput sequencing. The results showed differences in bacterial community composition between the upper and lower reaches of glacial runoff. The abundance of Actinobacteria, Firmicutes, Betaproteobacteria and Gammaproteobacteria in the upper reaches of glacial runoff was higher than that in the lower reaches. In contrast, the the abundance of Cyanobacteria and Alphaproteobacteria in the downstream of glacial runoff was higher than that in the upstream. In addition, we compared bacterial diversity and composition between glacial runoff areas and soils. The chart analysis showed that bacterial diversity in glacial soil was higher than that in the glacial runoff. Some typical bacteria in the soil, such as Actinobacteria, entered glacial runoff through contact between them. The abundance of Acidobacteria, Sphingobacterium and Flavobacterium was higher in glacial soil. Weighted correlation network analysis showed that the core bacteria in glacial runoff and glacial soil were typical bacteria in different habitats. Distance-based redundancy analysis revealed that NO 2 - -N was the most significant factor affecting the distribution of soil bacterial community, while NO 3 - -N was the most significant factor affecting the distribution of glacial runoff bacterial community.

scholarly journals Pavement Overrides the Effects of Tree Species on Soil Bacterial Communities

2021 ◽  
Vol 18 (4) ◽  
pp. 2168
Author(s):  
Yinhong Hu ◽  
Weiwei Yu ◽  
Bowen Cui ◽  
Yuanyuan Chen ◽  
Hua Zheng ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Bacterial Diversity ◽  
Community Composition ◽  
Tree Species ◽  
Bacterial Communities ◽  
Bacterial Community Composition ◽  
Soil Bacterial Community ◽  
Soil Bacterial Communities ◽  
Pine Stands ◽  
Soil Bacterial

Human disturbance and vegetation are known to affect soil microorganisms. However, the interacting effects of pavement and plant species on soil bacterial communities have received far less attention. In this study, we collected soil samples from pine (Pinus tabuliformis Carr.), ash (Fraxinus chinensis), and maple (Acer truncatum Bunge) stands that grew in impervious, pervious, and no pavement blocks to investigate the way pavement, tree species, and their interaction influence soil bacterial communities by modifying soil physicochemical properties. Soil bacterial community composition and diversity were evaluated by bacterial 16S amplicon sequencing. The results demonstrated that soil bacterial community composition and diversity did differ significantly across pavements, but not with tree species. The difference in soil bacterial community composition across pavements was greater in pine stands than ash and maple stands. Soil bacterial diversity and richness indices decreased beneath impervious pavement in pine stands, and only bacterial richness indices decreased markedly in ash stands, but neither showed a significant difference across pavements in maple stands. In addition, bacterial diversity did not differ dramatically between pervious pavement and no pavement soil. Taken together, these results suggest that pavement overwhelmed the effects of tree species on soil bacterial communities, and had a greater effect on soil bacterial communities in pine stands, followed by ash and maple stands. This study highlights the importance of anthropogenic disturbance, such as pavement, which affects soil microbial communities.

scholarly journals Effects of Different Vegetable Rotations on the Bacterial Community Structure and Tomato Growth in a Continuous Tomato Cropping Substrate

2021 ◽  
Author(s):  
Li Jin ◽  
Jihua Yu ◽  
Ning Jin ◽  
Jianming Xie ◽  
Yue Wu ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Diversity ◽  
Crop Rotation ◽  
Bacterial Communities ◽  
Bacterial Community Structure ◽  
Total N ◽  
Healthy Growth ◽  
Dominant Bacteria ◽  
Substrate Ph

Abstract Purpose In this study, MiSeq pyrosequencing was used to analyze the effects of different vegetable rotations on the bacterial diversity and community structure in a substrate that was used for continuous tomato cropping (CK). Methods The vegetable rotations tested were cabbage/tomato (B), kidney bean/tomato (D), and celery/tomato (Q). Results The results revealed that the substrate bacterial diversity and richness of each crop rotation were higher than those of CK. The highest bacterial diversity was found in the B substrate, followed by the Q and D substrates. Further comparison showed that the bacterial community structure of Q substrate was significantly different to that of CK. Compared with the CK, the Q substrate had a significantly higher relative abundance of several dominant microflora, such as Acidobacteria, Chloroflexi, and Firmicutes. Additionally, the Q rotation significantly increased the abundance of beneficial bacteria, such as Actinobacteria_unclassified and Anaerolineaceae_unclassified. A redundancy analysis showed that Most dominant bacteria correlated positively with the substrate pH, total N, and alkali-hydrolyzable N but negatively with the available P, available K, total P, total K, and organic matter contents and substrate EC. The substrates after crop rotation improved the growth and physiological condition of the subsequent tomato plants, among which those from the Q rotation performed the best. Conclusion Therefore, celery rotation not only increased the richness and diversity of bacterial communities in the substrate but also significantly increased the richness of the beneficial bacterial communities, allowing better maintenance of the substrate microenvironment for the healthy growth of crops.

scholarly journals Bacterial diversity and predicted enzymatic function in a multipurpose surface water system – from wastewater effluent discharges to drinking water production

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Ananda Tiwari ◽  
Anna-Maria Hokajärvi ◽  
Jorge Santo Domingo ◽  
Michael Elk ◽  
Balamuralikrishna Jayaprakash ◽  
...  
Keyword(s):  
Drinking Water ◽  
Surface Water ◽  
Bacterial Community ◽  
Human Health ◽  
Bacterial Diversity ◽  
Water Samples ◽  
Bacterial Communities ◽  
Treatment Process ◽  
Pollution Sources ◽  
Production Well

Abstract Background Rivers and lakes are used for multiple purposes such as for drinking water (DW) production, recreation, and as recipients of wastewater from various sources. The deterioration of surface water quality with wastewater is well-known, but less is known about the bacterial community dynamics in the affected surface waters. Understanding the bacterial community characteristics —from the source of contamination, through the watershed to the DW production process—may help safeguard human health and the environment. Results The spatial and seasonal dynamics of bacterial communities, their predicted functions, and potential health-related bacterial (PHRB) reads within the Kokemäenjoki River watershed in southwest Finland were analyzed with the 16S rRNA-gene amplicon sequencing method. Water samples were collected from various sampling points of the watershed, from its major pollution sources (sewage influent and effluent, industrial effluent, mine runoff) and different stages of the DW treatment process (pre-treatment, groundwater observation well, DW production well) by using the river water as raw water with an artificial groundwater recharge (AGR). The beta-diversity analysis revealed that bacterial communities were highly varied among sample groups (R = 0.92, p <  0.001, ANOSIM). The species richness and evenness indices were highest in surface water (Chao1; 920 ± 10) among sample groups and gradually decreased during the DW treatment process (DW production well; Chao1: 320 ± 20). Although the phylum Proteobacteria was omnipresent, its relative abundance was higher in sewage and industrial effluents (66–80%) than in surface water (55%). Phyla Firmicutes and Fusobacteria were only detected in sewage samples. Actinobacteria was more abundant in the surface water (≥13%) than in other groups (≤3%). Acidobacteria was more abundant in the DW treatment process (≥13%) than in others (≤2%). In total, the share of PHRB reads was higher in sewage and surface water than in the DW treatment samples. The seasonal effect in bacterial communities was observed only on surface water samples, with the lowest diversity during summer. Conclusions The low bacterial diversity and absence of PHRB read in the DW samples indicate AGR can produce biologically stable and microbiologically safe drinking water. Furthermore, the significantly different bacterial communities at the pollution sources compared to surface water and DW samples highlight the importance of effective wastewater treatment for protecting the environment and human health.

scholarly journals Effects of Simulated Nitrogen Deposition on the Bacterial Community of Urban Green Spaces

2021 ◽  
Vol 11 (3) ◽  
pp. 918
Author(s):  
Lingzi Mo ◽  
Augusto Zanella ◽  
Xiaohua Chen ◽  
Bin Peng ◽  
Jiahui Lin ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Bacterial Diversity ◽  
Negative Impact ◽  
Bacterial Community Composition ◽  
N Deposition ◽  
Soil Bacterial Community ◽  
Rrna Gene ◽  
Soil Dna ◽  
Urban Green ◽  
Soil Bacterial

Continuing nitrogen (N) deposition has a wide-ranging impact on terrestrial ecosystems. To test the hypothesis that, under N deposition, bacterial communities could suffer a negative impact, and in a relatively short timeframe, an experiment was carried out for a year in an urban area featuring a cover of Bermuda grass (Cynodon dactylon) and simulating environmental N deposition. NH4NO3 was added as external N source, with four dosages (N0 = 0 kg N ha−2 y−1, N1 = 50 kg N ha−2 y−1, N2 = 100 kg N ha−2 y−1, N3 = 150 kg N ha−2 y−1). We analyzed the bacterial community composition after soil DNA extraction through the pyrosequencing of the 16S rRNA gene amplicons. N deposition resulted in soil bacterial community changes at a clear dosage-dependent rate. Soil bacterial diversity and evenness showed a clear trend of time-dependent decline under repeated N application. Ammonium nitrogen enrichment, either directly or in relation to pH decrease, resulted in the main environmental factor related to the shift of taxa proportions within the urban green space soil bacterial community and qualified as a putative important driver of bacterial diversity abatement. Such an impact on soil life induced by N deposition may pose a serious threat to urban soil ecosystem stability and surrounding areas.

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