scholarly journals Assessing and genotyping threatened staghorn coral Acropora cervicornis nurseries during restoration in southeast Dominican Republic

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8863
Author(s):  
Johanna Calle-Triviño ◽  
Renata Rivera-Madrid ◽  
María Geovana León-Pech ◽  
Camilo Cortés-Useche ◽  
Rita Inés Sellares-Blasco ◽  
...  
Keyword(s):  
Dominican Republic ◽  
Coral Species ◽  
Genetic Characterization ◽  
Acropora Cervicornis ◽  
Critically Endangered Species ◽  
Hermodice Carunculata ◽  
Excellent Candidate ◽  
Site Restoration ◽  
The Mean

Acropora cervicornis is a structurally and functionally important Caribbean coral species. Since the 1980s, it has suffered drastic population losses with no signs of recovery and has been classified as a critically endangered species. Its rapid growth rate makes it an excellent candidate for coral restoration programs. In 2011, the Fundación Dominicana de Estudios Marinos (Dominican Marine Studies Foundation, FUNDEMAR) began an A. cervicornis restoration program in Bayahibe, southeast Dominican Republic. In this study, we present the methodology and results of this program from its conception through 2017, a preliminary analysis of the strong 2016 and 2017 cyclonic seasons in the greater Caribbean, and a genetic characterization of the “main nursery”. The mean survival of the fragments over 12 months was 87.45 ± 4.85% and the mean productivity was 4.01 ± 1.88 cm year−1 for the eight nurseries. The mean survival of six outplanted sites over 12 months was 71.55 ± 10.4%, and the mean productivity was 3.03 ± 1.30 cm year−1. The most common cause of mortality during the first 12 months, in both nurseries and outplanted sites, was predation by the fireworm, Hermodice carunculata. We identified 32 multilocus genotypes from 145 total analyzed individuals. The results and techniques described here will aid in the development of current and future nursery and outplanted site restoration programs.

Genetic characterization of local goats of Karnataka by microsatellite marker analysis

2018 ◽  
Author(s):  
R. Jayashree ◽  
M. R. Jayashankar ◽  
C. S. Nagaraja ◽  
Isloor Shrikrishna ◽  
K. Satyanarayana
Keyword(s):  
Microsatellite Marker ◽  
Genomic Dna ◽  
Genetic Characterization ◽  
Expected Heterozygosity ◽  
Marker Analysis ◽  
Goat Population ◽  
Pcr Products ◽  
The Mean ◽  
Microsatellite Marker Analysis

The diversity status of local goats of Karnataka was studied by using microsatellite marker analysis. The genomic DNA from unrelated local goats were PCR- amplified with a panel of 23 microsatellite markers. Microsatellite PCR products were multiplexed and run on capillary based genetic analyser and the raw data obtained was analysed. Totally 158 alleles were observed and the number of alleles ranged from three (ILSTS005 and OarJMP29) to 13 (RM088). The number of effective alleles ranged from 2.25 (ILSTS005) to 8.40 (RM088) in all the 23 loci studied. The mean observed heterozygosity (Ho) was 0.4698±0.2214 [range 0 (ETH225) to 0.8462 (ILSTS034)] and the mean expected heterozygosity (He) was 0.7471± 0.1098 [range 0.5656 (ILSTS005) to 0.9138 (SRCRSP 8)] indicating the heterogenous nature of the local goat population of Karnataka.

scholarly journals First record of the fireworm Hermodice carunculata preying on colonies of the threatened staghorn coral Acropora Cervicornis in the southeastern outplanting sites of the Dominican Republic

2017 ◽  
pp. 97-98 ◽  
Author(s):  
Johanna Calle-Triviño ◽  
Camilo Cortés-Useche ◽  
Rita Sellares ◽  
Jesús Ernesto Arias González
Keyword(s):  
Dominican Republic ◽  
Negative Impact ◽  
First Record ◽  
Acropora Cervicornis ◽  
Living Tissue ◽  
First Report ◽  
Hermodice Carunculata

Several fireworms Hermodice carunculata (Pallas, 1766) were observed feeding on coral colonies or transplanted fragments of the threatened staghorn coral Acropora cervicornis (Lamarck, 1816), in outplanting sites at Bayahibe, Dominican Republic. The fireworm H. carunculata has a highly negative impact on A. cervicornis populations, causing several lesions on the living tissue of branches and partial or complete mortality at the outplanting sites. This is the first report of predation on transplanted colonies in a coral restoration program in the Dominican Republic.

scholarly journals Genetic characterization of a brangus-ibage cattle population: biochemical polymorphisms and reproductive efficiency

2000 ◽  
Vol 30 (5) ◽  
pp. 803-807
Author(s):  
Luiz Ernani Henkes ◽  
Lídia Gonzalez Papadopolis ◽  
Clara Sabina Steigleder ◽  
José Carlos Ferrugem Moraes ◽  
Tania de Azevedo Weimer
Keyword(s):  
Genetic Characterization ◽  
Blood Protein ◽  
Reproductive Efficiency ◽  
Phosphogluconate Dehydrogenase ◽  
Glucose Phosphate ◽  
Biochemical Polymorphisms ◽  
The Mean ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Good Agreement

Biochemical techniques were used to investigate the genetic variability in a Brangus-Ibage population by determining allele frequencies of 18 blood protein systems: Hemogloin beta-Chain (Hb), Albumin (Alb), Amylase (Am), Transferrin (Tf), Carbonic Anhydrase (CA), Ceruloplasmin (Cp), Malic Enzyme (ME), Diaphorase I and II (Dia I and Dia II), Slow Alpha 2 Macroglobulin (Ap), Acid Phosphatase (ACP), Esterase B and D (EstB and EstD), Phosphogluconate Dehydrogenase (PGD), Glucose-6-Phosphate Dehydrogenase (G-6-PD), Glucose-Phosphate-Isomerase (GPI), Superoxide Dismutase (SOD) and Glyoxalase I (GLO). The percentage of polymorphic loci were estimated at 0.27, the mean number of alleles was 1.33 and the mean heterozygosity was 0.07. There was a good agreement between expected and observed heterozygosity values. The population was in agreement with Hardy-Weinberg expectations in all systems. Reproductive records allowed to estimate three parameters of reproductive efficiency: mean age at first calving (1152.15 ± 166.60 days), mean calving interval (539.23 ± 124.10 days) and mean weight at first calving (391.02 ± 37.59kg). No relationship was found between reproductive efficiency and genetic systems.

scholarly journals Genetic characterization of farmed and wild populations of African catfish (Clarias gariepinus Burchell, 1822) using the random amplified polymorphic marker

2020 ◽  
Vol 65 (4) ◽  
pp. 375-389
Author(s):  
Sa`adu Suleiman ◽  
Mohammed Diyaware ◽  
Mohammed Aliyu ◽  
Zanna Mohammed
Keyword(s):  
Genetic Relatedness ◽  
Genetic Characterization ◽  
Gene Diversity ◽  
Clarias Gariepinus ◽  
Polymorphic Marker ◽  
African Catfish ◽  
Wild Populations ◽  
In The Wild ◽  
The Mean

The genetic characterization of Clarias gariepinus was conducted in this study. Thirty (30) C. gariepinus specimens were collected, fifteen (15) each from the wild and farms in northeastern Nigeria for their genetic relatedness and diversity using the RAPD markers. DNA extraction from the blood sample was performed using the Gene Jet Genomic DNA Purification Kit. Five primers were used in employing PCR and amplified 402 RAPD bands from the four strains of Clarias gariepinus. Three hundred and six (76.12%) bands were polymorphic while 86 (21.39%) were monomorphic. The percentage of polymorphism obtained from farmed and wild populations ranged from 58 (47.3%) to 69 (75.9%), respectively. The polymorphic bands per loci within the populations ranged from 67.9% to 82.0%. The mean numbers of inbreeding coefficient (FIS) were 0.083 and 0.053 in the farmed and wild populations. Gene diversity values within farmed and wild populations (Ht) were 0.4522 and 0.4018. The mean genetic differentiation (FST) ranged between (0.203) in the farmed and 0.129 in the wild populations. The analysis of molecular variance revealed that there was 96% of genetic variance within the population and 4% among the population. The genetic identity and distance of four populations were 0.9490 and 0.1038, respectively. The phylogenetic measure has shown that the four strains were divided into two clusters at approximately 0.089 similarity levels. The result indicated a significant level of genetic variation and minimal dendrogram separation in Clarias gariepinus in northeastern Nigeria. Conclusively, this information will be a useful tool for the genetic and breeding program of Clarias gariepinus.

scholarly journals Assessment of genetic diversity of Busa cattle from Bosnia and Herzegovina using microsatellite DNA markers

2011 ◽  
Vol 63 (4) ◽  
pp. 1077-1085
Author(s):  
Biljana Rogic ◽  
Lidija Tomic ◽  
B. Vazic ◽  
M. Jelic ◽  
S. Jovanovic ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Bosnia And Herzegovina ◽  
Genetic Characterization ◽  
Management Strategies ◽  
Cattle Breed ◽  
Microsatellite Dna Markers ◽  
The Mean ◽  
Two Populations ◽  
Conservation And Management

The variability of 21 microsatellite loci was analyzed in order to estimate the genetic diversity of the Busa cattle breed. A total of 50 animals involved in the study were divided into two groups: Busa from eastern Herzegovina and Busa from western Herzegovina. The mean number of alleles per locus was 6.6. The average expected heterozygosities were 0.6885 and 0.6212 in the eastern and western populations, respectively. The observed heterozygosity values were 0.6579 and 0.6336, in eastern and western population, respectively. The degree of population differentiation (FST) ranged from 0.008 (for ILSTS006 locus) to 0.242 (for BM1818 locus), with a mean of 0.112. Cluster analysis showed that the two populations were clearly clustered into two distinct clades. This report represents the first genetic characterization of pure Busa cattle from Bosnia and Herzegovina. The obtained results are important for the future development of conservation and management strategies for this cattle breed.

scholarly journals Primer Note: A novel set of EST-SSR markers in Tamarix: a resource to characterize this genus

2013 ◽  
Vol 62 (1-6) ◽  
pp. 104-109 ◽  
Author(s):  
S. Terzoli ◽  
E. Cattan ◽  
M. Sabatti ◽  
R. Valentini ◽  
A. Zilberstain ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Expressed Sequence Tag ◽  
Genetic Characterization ◽  
Ecological Impact ◽  
Expected Heterozygosity ◽  
Desert Habitat ◽  
The Mean ◽  
Expressed Sequence ◽  
Simple Sequence

Abstract Both the negative and positive ecological impact of Tamarix plants is controversial, and thus a more comprehensive understanding is necessary. Tamarisks are invasive in many countries but the inter-specific transferability that characterizes simple sequence repeats (SSRs) could be harnessed to track the spread of specific genotypes or to study invasive populations. Thirteen polymorphic SSR markers, derived from expressed sequence tag (EST), were identified by first screening 26 samples of T. aphylla, T. jordanis, T. nilotica, and T. tetragyna and then 33 unidentified tamarisks from Yotvata, Israel. The mean number of alleles per locus ranged from two to 14 and the mean expected heterozygosity was 0.415. These EST-SSR markers will undoubtedly be useful in the genetic characterization of the genus Tamarix due to their high cross-species transferability which enables the estimation of the genetic diversity among and within different species, that are adapted to the same desert habitat under severe environmental constraints.

Histopathological and genetic characterization of colorectal mucinous carcinoma

2001 ◽  
Vol 120 (5) ◽  
pp. A166-A166
Author(s):  
S FUJII ◽  
T KUSAKA ◽  
T KAIHARA ◽  
Y UEDA ◽  
T CHIBA ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Mucinous Carcinoma

Genetic characterization of high hyperdiploidy in childhood acute lymphoblastic leukemia

2009 ◽  
Vol 221 (03) ◽  
Author(s):  
R Vagkopoulou ◽  
C Eckert ◽  
U Ungethüm ◽  
G Körner ◽  
M Stanulla ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Genetic Characterization ◽  
Lymphoblastic Leukemia ◽  
Childhood Acute Lymphoblastic Leukemia

TBE in Sweden

2020 ◽  
Keyword(s):  
Genetic Characterization ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Second Phase ◽  
Serum Samples ◽  
Tick Borne Encephalitis ◽  
Specific Immunoglobulin ◽  
Tick Borne Encephalitis Virus ◽  
First Time

Tick-borne encephalitis virus (TBEV) was isolated for the first time in Sweden in 1958 (from ticks and from 1 tick-borne encephalitis [TBE] patient).1 In 2003, Haglund and colleagues reported the isolation and antigenic and genetic characterization of 14 TBEV strains from Swedish patients (samples collected 1991–1994).2 The first serum sample, from which TBEV was isolated, was obtained 2–10 days after onset of disease and found to be negative for anti-TBEV immunoglobulin M (IgM) by enzyme-linked immunosorbent assay (ELISA), whereas TBEV-specific IgM (and TBEV-specific immunoglobulin G/cerebrospinal fluid [IgG/CSF] activity) was demonstrated in later serum samples taken during the second phase of the disease.

Clinical and genetic characterization of two cases of central hypogonadism in Klinefelter syndrome

2020 ◽  
Author(s):  
Rita Indirli ◽  
Biagio Cangiano ◽  
Eriselda Profka ◽  
Elena Castellano ◽  
Giovanni Goggi ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Klinefelter Syndrome
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