Molecular Characteristics and Risk Factors of Bloodstream Infection Caused by Escherichia coli ST131 in Southeast China

2022 ◽  
Vol 68 (01/2022) ◽  
Author(s):  
Yanfang Lu ◽  
Jiangqing Huang ◽  
Jiaqi Su ◽  
Huiyu Chen ◽  
Zhihui Wu ◽  
...  
Keyword(s):  
Risk Factors ◽  
Escherichia Coli ◽  
Bloodstream Infection ◽  
Molecular Characteristics ◽  
Southeast China

scholarly journals Extended-spectrum and AmpC β-lactamase-producing Escherichia coli in broilers and people living and/or working on broiler farms: prevalence, risk factors and molecular characteristics

2014 ◽  
Vol 69 (10) ◽  
pp. 2669-2675 ◽  
Author(s):  
P. M. C. Huijbers ◽  
E. A. M. Graat ◽  
A. P. J. Haenen ◽  
M. G. van Santen ◽  
A. van Essen-Zandbergen ◽  
...  
Keyword(s):  
Risk Factors ◽  
Escherichia Coli ◽  
Molecular Characteristics ◽  
Extended Spectrum

scholarly journals A prospective study of Escherichia coli bloodstream infection among adolescents and adults in northern Tanzania

2019 ◽  
Vol 114 (5) ◽  
pp. 378-384
Author(s):  
Deng B Madut ◽  
Matthew P Rubach ◽  
Nathaniel Kalengo ◽  
Manuela Carugati ◽  
Michael J Maze ◽  
...  
Keyword(s):  
Risk Factors ◽  
Escherichia Coli ◽  
Blood Culture ◽  
Bloodstream Infection ◽  
Antimicrobial Susceptibility ◽  
Female Gender ◽  
Sub Saharan Africa ◽  
E Coli ◽  
High Resource ◽  
Northern Tanzania

ABSTRACT Background Characterization of the epidemiology of Escherichia coli bloodstream infection (BSI) in sub-Saharan Africa is lacking. We studied patients with E. coli BSI in northern Tanzania to describe host risk factors for infection and to describe the antimicrobial susceptibility of isolates. Methods Within 24 h of admission, patients presenting with a fever at two hospitals in Moshi, Tanzania, were screened and enrolled. Cases were patients with at least one blood culture yielding E. coli and controls were those without E. coli isolated from any blood culture. Logistic regression was used to identify host risk factors for E. coli BSI. Results We analyzed data from 33 cases and 1615 controls enrolled from 2007 through 2018. The median (IQR) age of cases was 47 (34–57) y and 24 (72.7%) were female. E. coli BSI was associated with (adjusted OR [aOR], 95% CI) increasing years of age (1.03, 1.01 to 1.05), female gender (2.20, 1.01 to 4.80), abdominal tenderness (2.24, 1.06 to 4.72) and urinary tract infection as a discharge diagnosis (3.71, 1.61 to 8.52). Of 31 isolates with antimicrobial susceptibility results, the prevalence of resistance was ampicillin 29 (93.6%), ceftriaxone three (9.7%), ciprofloxacin five (16.1%), gentamicin seven (22.6%) and trimethoprim-sulfamethoxazole 31 (100.0%). Conclusions In Tanzania, host risk factors for E. coli BSI were similar to those reported in high-resource settings and resistance to key antimicrobials was common.

scholarly journals Carriage of antimicrobial resistant Escherichia coli in dogs: Prevalence, associated risk factors and molecular characteristics

2017 ◽  
Vol 199 ◽  
pp. 23-30 ◽  
Author(s):  
Amy L. Wedley ◽  
Susan Dawson ◽  
Thomas W. Maddox ◽  
Karen P. Coyne ◽  
Gina L. Pinchbeck ◽  
...  
Keyword(s):  
Risk Factors ◽  
Escherichia Coli ◽  
Molecular Characteristics ◽  
Associated Risk Factors

scholarly journals Fecal Carriage of Extended-Spectrum-β-Lactamase/AmpC-Producing Escherichia coli in Horses

2020 ◽  
Vol 86 (8) ◽  
Author(s):  
Joost Hordijk ◽  
Evangelia Farmakioti ◽  
Lidwien A. M. Smit ◽  
Birgitta Duim ◽  
Haitske Graveland ◽  
...  
Keyword(s):  
Risk Factors ◽  
Escherichia Coli ◽  
The Netherlands ◽  
Potential Risk ◽  
Molecular Characteristics ◽  
Horse Population ◽  
Nationwide Study ◽  
Extended Spectrum ◽  
Animal Populations ◽  
Potential Risk Factors

ABSTRACT A nationwide study on the occurrence of extended-spectrum β-lactamase (ESBL)/AmpC in nonhospitalized horses in the Netherlands was performed. Molecular characterization was done, and questionnaires were analyzed to identify factors associated with carriage. In total, 796 horse owners were approached; 281 of these submitted a fecal sample from their horse(s), resulting in 362 samples. All samples were cultured qualitatively in Luria-Bertani (LB) broth and subsequently on MacConkey agar, both supplemented with 1 mg/liter cefotaxime (LB+ and MC+). Positive samples were subsequently cultured quantitatively on MC+. Initial extended-spectrum-β-lactamase (ESBL)/AmpC screening was performed by PCR, followed by whole-genome sequencing on selected strains. Associations between ESBL/AmpC carriage and questionnaire items were analyzed using a univariate generalized estimating equation (GEE) regression analysis, followed by a multiple GEE model for relevant factors. In total, 39 of 362 samples (11%) were determined to be positive for ESBL/AmpC. blaCTX-M-1-carrying isolates were obtained from 77% of positive samples (n = 30). Other ESBL/AmpC genes observed included blaCTX-M-2, blaCTX-M-14, blaCTX-M-15, blaCTX-M-32, blaSHV-12, blaCMY-2, and blaACT-10. A high association between the presence of blaCTX-M-1 and IncHI1 plasmids was observed (46% of samples; n = 18). Based on core genome analysis (n = 48 isolates), six Escherichia coli clusters were identified, three of which represented 80% of the isolates. A negative association between ESBL/AmpC carriage and horses being in contact with other horses at a different site was observed. The presence of a dog on the premises and housing in a more densely human-populated region were positively associated. IMPORTANCE Extended-spectrum β-lactamases (ESBLs) are widespread in human and animal populations and in the environment. Many different ESBL variants exist. The dissemination of ESBLs within and between populations and the environment is also largely influenced by genetic mobile elements (e.g., plasmids) that facilitate spread of these ESBLs. In order to identify potential attributable ESBL sources for, e.g., the human population, it is important to identify the different ESBL variants, the bacteria carrying them, and the potential risk factors for ESBL carriage from other potential sources. This nationwide study focuses on ESBL carriage in the open horse population and investigated the molecular characteristics, geographical distribution throughout the Netherlands, and potential risk factors for fecal ESBL carriage in horses. These data can be used for future attribution studies in order to reduce potential transmission of ESBL-producing bacteria between sources.

Risk Factors for and Outcomes of Bloodstream Infection Caused by Extended-Spectrum ss-Lactamase-Producing Escherichia coli and Klebsiella Species in Children

PEDIATRICS ◽  
2005 ◽  
Vol 115 (4) ◽  
pp. 942-949 ◽  
Author(s):  
T. E. Zaoutis ◽  
M. Goyal ◽  
J. H. Chu ◽  
S. E. Coffin ◽  
L. M. Bell ◽  
...  
Keyword(s):  
Risk Factors ◽  
Escherichia Coli ◽  
Bloodstream Infection ◽  
Klebsiella Species ◽  
Extended Spectrum

Prevalence, risk factors and molecular characteristics of Shiga toxin-producing Escherichia coli in beef abattoirs in Gauteng, South Africa

Food Control ◽  
2020 ◽  
pp. 107746
Author(s):  
Libby O. Onyeka ◽  
Abiodun A. Adesiyun ◽  
Karen H. Keddy ◽  
Ayanda Manqele ◽  
Evelyn Madoroba ◽  
...  
Keyword(s):  
Risk Factors ◽  
Escherichia Coli ◽  
South Africa ◽  
Shiga Toxin ◽  
Molecular Characteristics

scholarly journals Bloodstream infections initiated by ESBL-producing Escherichia coli in neonates and infants at two hospitals in Yaoundé, Cameroon

2021 ◽  
Vol 15 (3) ◽  
pp. 1222-1233
Author(s):  
Carole Suzie Lacmago Titsamp ◽  
Hortense Kamga Gonsu ◽  
Simon Ngamli Fewou
Keyword(s):  
Risk Factors ◽  
Escherichia Coli ◽  
Bloodstream Infection ◽  
Antibiotic Susceptibility ◽  
Klebsiella Oxytoca ◽  
Blood Cultures ◽  
Susceptibility Test ◽  
Coagulase Negative Staphylococci ◽  
Antibiotic Susceptibility Test ◽  
Neonates And Infants

Early diagnosis and probabilistic antibiotic therapy based on known bacterial ecology and antibiotic sensibility can reduce mortality and morbidity in pathologies caused by a bacterial infection. This study aimed at determining the prevalence and risk factors of extended-spectrum β-lactamases (ESBLs)-producing Escherichia coli isolated from blood cultures of neonates and infants population. We conducted a cross-sectional study during which pathogenic bloodstream isolates were identified. Antibiotic susceptibility test was performed on Escherichia coli isolates and phenotypic confirmation of ESBL production by Escherichia coli was performed by a double-disc synergy test. Over the course of this study, 298 blood cultures were performed and 129 (43.3%) positive cultures were obtained. Of the 129 bacterial isolates, 90 (69.7%) were Escherichia coli and 39 (30.2%) were other bacteria strains that included Klebsiella oxytoca, Streptococcus pneumonia, and Coagulase-negative staphylococci. Antibiotic susceptibility test indicated that Escherichia coli isolates were resistant to cephalosporin, penicillin, sulfonamide, and aminoglycoside antibiotic families. Further analysis indicated that 31 (34.4%) Escherichia coli strains were ESBL producers and risk factors for bloodstream infection by ESBL-producing Escherichia coli were prior to exposure to antibiotics and immune system depression. These findings clearly extend our understanding of the type of resistant initiated by ESBL-producing Escherichia coli in bloodstream infection of neonates, and infants and also provides useful information that can guide the establishment of an efficient therapeutic strategy for the community- and hospital-acquired bloodstream infection.

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