Expression of DNA Gyrase at mRNA Levels and Antibacterial Effect of Fluoroquinolone-Derived Compounds on Two Antibiotic Resistant Bacteria; Staphylococcus aureus and Enterococcus faecalis

The aim of this study was to determine the antibacterial activity of Cathormion umbellatum extracts against seven antibiotic-resistant bacteria. The pods, leaves and branches of C. umbellatum were extracted with ethanol and methanol. The disc diffusion assay was used to screen the antibacterial activity and broth microdilution and colorimetric assay were used to measure the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values. The result indicated that the highest inhibition zone (11 mm) was presented in ethanolic pods extract against multidrug resistance Klebsiella pneumoniae. The lowest MIC value of 0.05 mg/mL was obtained from branch extracted with ethanol against colistin resistant Pseudomonas aeruginosa. The lowest MBC values of 1.56 mg/mL were obtained when using C. umbellatum leaves extracted with methanol against all test antibiotic-resistant bacteria. This is the first report presented C. umbellatum extracts have the potential to eliminate antibiotic-resistant bacteria in patients. These findings show the antibacterial effect of C. umbellatum.

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Drug-resistant Staphylococcus aureus bacteria detection by combining surface-enhanced Raman spectroscopy (SERS) and deep learning techniques

Scientific Reports ◽

10.1038/s41598-021-97882-4 ◽

2021 ◽

Vol 11 (1) ◽

Cited By ~ 1

Author(s):

Fatma Uysal Ciloglu ◽

Abdullah Caliskan ◽

Ayse Mine Saridag ◽

Ibrahim Halil Kilic ◽

Mahmut Tokmakci ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Raman Spectroscopy ◽

Deep Learning ◽

Surface Enhanced Raman Spectroscopy ◽

Resistant Bacteria ◽

High Signal ◽

Antibiotic Resistant Bacteria ◽

Antibiotic Resistant ◽

Surface Enhanced ◽

Surface Enhanced Raman

AbstractOver the past year, the world's attention has focused on combating COVID-19 disease, but the other threat waiting at the door—antimicrobial resistance should not be forgotten. Although making the diagnosis rapidly and accurately is crucial in preventing antibiotic resistance development, bacterial identification techniques include some challenging processes. To address this challenge, we proposed a deep neural network (DNN) that can discriminate antibiotic-resistant bacteria using surface-enhanced Raman spectroscopy (SERS). Stacked autoencoder (SAE)-based DNN was used for the rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) bacteria using a label-free SERS technique. The performance of the DNN was compared with traditional classifiers. Since the SERS technique provides high signal-to-noise ratio (SNR) data, some subtle differences were found between MRSA and MSSA in relative band intensities. SAE-based DNN can learn features from raw data and classify them with an accuracy of 97.66%. Moreover, the model discriminates bacteria with an area under curve (AUC) of 0.99. Compared to traditional classifiers, SAE-based DNN was found superior in accuracy and AUC values. The obtained results are also supported by statistical analysis. These results demonstrate that deep learning has great potential to characterize and detect antibiotic-resistant bacteria by using SERS spectral data.

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Evaluation of Sustained Antibacterial Effect of Alcohol-Based Disinfectant Containing Monovalent Copper Compound Against Antibiotic-Resistant Bacteria

American Journal of Infection Control ◽

10.1016/j.ajic.2020.06.116 ◽

2020 ◽

Vol 48 (8) ◽

pp. S19

Author(s):

Yoshie Fujimori ◽

Ayumi Fukuyo ◽

Tomokazu Nagao ◽

Hajime Kanamori

Keyword(s):

Antibacterial Effect ◽

Resistant Bacteria ◽

Copper Compound ◽

Antibiotic Resistant Bacteria ◽

Antibiotic Resistant

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Synthesis and antibiofilm activity of 1,2,3-triazole-pyridine hybrids against Staphylococcus aureus (MRSA)

New Journal of Chemistry ◽

10.1039/d1nj00773d ◽

2021 ◽

Author(s):

Tamer El Malah ◽

Hanan A. Soliman ◽

Bahaa A. Hemdan ◽

Randa E. Abdel Mageid ◽

Hany Nour

Keyword(s):

Staphylococcus Aureus ◽

Human Health ◽

Resistant Bacteria ◽

Antibiofilm Activity ◽

Antibiotic Resistant Bacteria ◽

Potential Threat ◽

Antibiotic Resistant ◽

Dihydropyridine Derivatives

Antibiotic-resistant bacteria are emerging at an alarming rate, posing a potential threat to human health. We synthesised alkyne-functionalised pyridines 3 and 4 via alkylation of substituted 2-oxo-1,2-dihydropyridine derivatives 1 and...

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The impact of methicillin-resistant Staphylococcus aureus on ENT practice

The Journal of Laryngology & Otology ◽

10.1017/s0022215106001393 ◽

2006 ◽

Vol 120 (9) ◽

pp. 713-717 ◽

Cited By ~ 12

Author(s):

I J Nixon ◽

B J G Bingham

Keyword(s):

Staphylococcus Aureus ◽

Infection Control ◽

Methicillin Resistant Staphylococcus Aureus ◽

Methicillin Resistance ◽

Resistant Bacteria ◽

Antibiotic Resistant Bacteria ◽

Antibiotic Resistant ◽

Methicillin Resistant ◽

The Impact

Antibiotic-resistant bacteria are increasingly common and present a major problem for the modern day ENT surgeon. This article reviews the development of methicillin resistance in Staphylococcus aureus and how it has come to affect ENT practice. We look at the evidence behind measures taken to help deal with methicillin-resistant Staphylococcus aureus (MRSA) and to prevent its spread. We go on to suggest a departmental guideline for infection control, which we hope can be implemented to help deal with the problems created by MRSA.

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N-Heterocyclic dicarboxylic acids: Broad-spectrum inhibitors of metallo-β-lactamases with co-antibacterial effect against antibiotic-resistant bacteria

Bioorganic & Medicinal Chemistry Letters ◽

10.1016/j.bmcl.2012.06.074 ◽

2012 ◽

Vol 22 (16) ◽

pp. 5185-5189 ◽

Cited By ~ 37

Author(s):

Lei Feng ◽

Ke-Wu Yang ◽

Li-Sheng Zhou ◽

Jian-Min Xiao ◽

Xia Yang ◽

...

Keyword(s):

Broad Spectrum ◽

Antibacterial Effect ◽

Dicarboxylic Acids ◽

Resistant Bacteria ◽

Antibiotic Resistant Bacteria ◽

Antibiotic Resistant

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Discovery of Antibacterial Dietary Spices That Target Antibiotic-Resistant Bacteria

Microorganisms ◽

10.3390/microorganisms7060157 ◽

2019 ◽

Vol 7 (6) ◽

pp. 157 ◽

Cited By ~ 4

Author(s):

Dan Zhang ◽

Ren-You Gan ◽

Arakkaveettil Kabeer Farha ◽

Gowoon Kim ◽

Qiong-Qiong Yang ◽

...

Keyword(s):

Antibacterial Activity ◽

Antibacterial Effect ◽

Antioxidant Activities ◽

Antibacterial Properties ◽

Trolox Equivalent Antioxidant Capacity ◽

Resistant Bacteria ◽

Drug Resistant ◽

Antibiotic Resistant Bacteria ◽

Antibiotic Resistant ◽

Dietary Spices

Although spice extracts are well known to exhibit antibacterial properties, there is lack of a comprehensive evaluation of the antibacterial effect of spices against antibiotic-resistant bacteria. In the present study, ethanolic extracts from a total of 67 spices were comprehensively investigated for their in vitro antibacterial activities by agar well diffusion against two common food-borne bacteria, Staphylococcus aureus and Salmonella enteritidis, with multi-drug resistance. Results showed that S. aureus was generally more sensitive to spice extracts than S. enteritidis. Of the 67 spice extracts, 38 exhibited antibacterial activity against drug-resistant S. aureus, while only four samples were effective on drug-resistant S. enteritidis. In addition, 11 spice extracts with inhibition zones greater than 15 mm were further verified for their broad-spectrum antibacterial properties using another 10 drug-resistant S. aureus strains. It was found that five spice extracts, including galangal, fructus galangae, cinnamon, yellow mustard seed, and rosemary, exhibited the highest antibacterial capacity. Further cytotoxicity of these 11 spices was determined and LC50 values were found to be more than 100 μg/mL except for galangal, rosemary, and sage, whose LC50 values were 9.32 ± 0.83, 19.77 ± 2.17, and 50.54 ± 2.57, respectively. Moreover, the antioxidant activities (ferric-reducing antioxidant power (FRAP) and trolox equivalent antioxidant capacity (TEAC) values) and total phenolic content (TPC) of spice extracts were determined to establish possible correlations with the antibacterial activity. Although the antibacterial effect was positively correlated with the antioxidant activities and TPC, the correlation was weak (r < 0.5), indicating that the antibacterial activity could also be attributed to other components besides antioxidant polyphenols in the tested spice extracts. In conclusion, dietary spices are good natural sources of antibacterial agents to fight against antibiotic-resistant bacteria, with potential applications as natural food preservatives and natural alternatives to antibiotics in animal feeding.

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Inactivation of antibiotic-resistant bacteria by chlorine dioxide in soil and shifts in community composition

RSC Advances ◽

10.1039/c8ra07997h ◽

2019 ◽

Vol 9 (12) ◽

pp. 6526-6532 ◽

Cited By ~ 5

Author(s):

M. S. Wu ◽

X. Xu

Keyword(s):

Staphylococcus Aureus ◽

Community Composition ◽

Chlorine Dioxide ◽

Resistant Bacteria ◽

Antibiotic Resistant Bacteria ◽

Antibiotic Resistant ◽

Resistant Species ◽

And Shifts

Antibiotic-resistant bacteria are common widespread in soil and the most resistant species isStaphylococcus aureus.Sphingomonas,ArthrobacterandMassiliaare sensitive to ClO2.MicromonosporaceaeandThaumarchaeotaare more resistant to ClO2.

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