scholarly journals Emergence of genotype I of dengue virus serotype 3 during severe dengue epidemic in Sri Lanka, 2017

2021 ◽  
Author(s):  
Mya Myat Ngwe Tun ◽  
Rohitha Muthugala ◽  
Lakmali Rajamanthri ◽  
Takeshi Nabeshima ◽  
Corazon C. Buerano ◽  
...  
Keyword(s):  
Sri Lanka ◽  
Dengue Virus ◽  
Severe Dengue ◽  
Genotype I ◽  
Virus Serotype ◽  
Dengue Epidemic

scholarly journals Dengue Virus Serotype 4 Is Responsible for the Outbreak of Dengue in East Java City of Jember, Indonesia

Viruses ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 913
Author(s):  
Aryati Aryati ◽  
Billy J. Wrahatnala ◽  
Benediktus Yohan ◽  
May Fanny ◽  
Faradila K. N. Hakim ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Clinical Symptoms ◽  
Secondary Infection ◽  
Herd Immunity ◽  
Antibody Detection ◽  
Severe Dengue ◽  
Envelope Gene ◽  
Genotype I ◽  
Denv Serotypes ◽  
Virus Serotype

Outbreaks of dengue virus (DENV) in Indonesia have been mainly caused by the DENV serotype-1; -2; or -3. The DENV-4 was the least-reported serotype in Indonesia during the last five decades. We recently conducted a molecular epidemiology study of dengue in the Jember regency, East Java province, Indonesia. Dengue is endemic in the region and outbreaks occur annually. We investigated the clinical characteristics and etiology of dengue-like febrile illness in this regency to understand the disease dynamics. A total of 191 patients with clinical symptoms similar to dengue were recruited during an 11-month study in 2019–2020. Children accounted for the majority of cases and dengue burden was estimated in 41.4% of the cases based on NS1 antigen, viral RNA, and IgG/IgM antibody detection with the majority (73.4%) being primary infections. Secondary infection was significantly associated with a higher risk of severe dengue manifestation. All four DENV serotypes were detected in Jember. Strikingly, we observed the predominance of DENV-4, followed by DENV-3, DENV-1, and DENV-2. Genotype determination using Envelope gene sequence revealed the classification into Genotype I, Cosmopolitan Genotype, Genotype I, and Genotype II for DENV-1, -2, -3, and -4, respectively. The predominance of DENV-4 in Jember may be associated with a new wave of DENV infections and spread in a non-immune population lacking a herd-immunity to this particular serotype.

scholarly journals Dengue Fever and Severe Dengue in Barbados, 2008–2016

2020 ◽  
Vol 5 (2) ◽  
pp. 68
Author(s):  
Kirk Osmond Douglas ◽  
Sudip Kumar Dutta ◽  
Byron Martina ◽  
Fatih Anfasa ◽  
T. Alafia Samuels ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Dengue Fever ◽  
Small Sample Size ◽  
Health Planning ◽  
Case Fatality ◽  
Small Sample ◽  
Severe Dengue ◽  
Medical Attention ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Serotype

Analysis of the temporal, seasonal and demographic distribution of dengue virus (DENV) infections in Barbados was conducted using national surveillance data from a total of 3994 confirmed dengue cases. Diagnosis was confirmed either by DENV–specific real time reverse transcriptase polymerase chain reaction (rRT–PCR), or non–structural protein 1 (NS1) antigen or enzyme linked immunosorbent assay (ELISA) tests; a case fatality rate of 0.4% (10/3994) was observed. The dengue fever (DF) prevalence varied from 27.5 to 453.9 cases per 100,000 population among febrile patients who sought medical attention annually. DF cases occurred throughout the year with low level of transmission observed during the dry season (December to June), then increased transmission during rainy season (July to November) peaking in October. Three major dengue epidemics occurred in Barbados during 2010, 2013 and possibly 2016 with an emerging three–year interval. DF prevalence among febrile patients who sought medical attention overall was highest among the 10–19 years old age group. The highest DF hospitalisation prevalence was observed in 2013. Multiple serotypes circulated during the study period and Dengue virus serotype 2 (DENV–2) was the most prevalent serotype during 2010, whilst DENV–1 was the most prevalent serotype in 2013. Two DENV–1 strains from the 2013 DENV epidemic were genetically more closely related to South East Asian strains, than Caribbean or South American strains, and represent the first ever sequencing of DENV strains in Barbados. However, the small sample size (n = 2) limits any meaningful conclusions. DF prevalence was not significantly different between females and males. Public health planning should consider DENV inter–epidemic periodicity, the current COVID–19 pandemic and similar clinical symptomology between DF and COVID–19. The implementation of routine sequencing of DENV strains to obtain critical data can aid in battling DENV epidemics in Barbados.

scholarly journals Seroepidemiology of dengue virus infection among adults during the ending phase of a severe dengue epidemic in southern Taiwan, 2015

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Yu-Wen Chien ◽  
Hsiang-Min Huang ◽  
Tzu-Chuan Ho ◽  
Fan-Chen Tseng ◽  
Nai-Ying Ko ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Severe Dengue ◽  
Dengue Virus Infection ◽  
Southern Taiwan ◽  
Dengue Epidemic

scholarly journals Recent expansion of dengue virus serotype 3 in West Africa

2010 ◽  
Vol 15 (7) ◽  
Author(s):  
L Franco ◽  
A Di Caro ◽  
F Carletti ◽  
O Vapalahti ◽  
C Renaudat ◽  
...  
Keyword(s):  
West Africa ◽  
Dengue Virus ◽  
Cape Verde ◽  
The Past ◽  
Virus Serotype ◽  
Dengue Epidemic

Due to non-existing or limited surveillance in Africa, little is known about the epidemiology of dengue illness in the continent. Serological and virological data obtained from returning European travellers is a key complement to this often flawed information. In the past years, dengue 3 virus has emerged in West Africa and has been detected in travellers returning to Europe. The first dengue epidemic in Cape Verde with more than 17,000 cases from September to December 2009 demonstrated that dengue virus is still expanding worldwide to new territories.

scholarly journals Severe Dengue Epidemic, Sri Lanka, 2017

2020 ◽  
Vol 26 (4) ◽  
pp. 682-691 ◽  
Author(s):  
Hasitha A. Tissera ◽  
Bernard D.W. Jayamanne ◽  
Rajendra Raut ◽  
Sakunthala M.D. Janaki ◽  
Yesim Tozan ◽  
...  
Keyword(s):  
Sri Lanka ◽  
Severe Dengue ◽  
Dengue Epidemic

scholarly journals Functional Transplant of a Dengue Virus Serotype 3 (DENV3)-Specific Human Monoclonal Antibody Epitope into DENV1

2016 ◽  
Vol 90 (10) ◽  
pp. 5090-5097 ◽  
Author(s):  
William B. Messer ◽  
Boyd L. Yount ◽  
Scott R. Royal ◽  
Ruklanthi de Alwis ◽  
Douglas G. Widman ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Dengue Virus ◽  
Neutralizing Antibodies ◽  
Human Monoclonal Antibody ◽  
Viral Envelope ◽  
Severe Dengue ◽  
Denv Serotypes ◽  
The Core ◽  
Antibody Epitope ◽  
Virus Serotype

ABSTRACTThe four dengue virus (DENV) serotypes, DENV1 through 4, are endemic throughout tropical and subtropical regions of the world. While first infection confers long-term protective immunity against viruses of the infecting serotype, a second infection with virus of a different serotype carries a greater risk of severe dengue disease, including dengue hemorrhagic fever and dengue shock syndrome. Recent studies demonstrate that humans exposed to DENV infections develop neutralizing antibodies that bind to quaternary epitopes formed by the viral envelope (E) protein dimers or higher-order assemblies required for the formation of the icosahedral viral envelope. Here we show that the quaternary epitope target of the human DENV3-specific neutralizing monoclonal antibody (MAb) 5J7 can be partially transplanted into a DENV1 strain by changing the core residues of the epitope contained within a single monomeric E molecule. MAb 5J7 neutralized the recombinant DENV1/3 strain in cell culture and was protective in a mouse model of infection with the DENV1/3 strain. However, the 5J7 epitope was only partially recreated by transplantation of the core residues because MAb 5J7 bound and neutralized wild-type (WT) DENV3 better than the DENV1/3 recombinant. Our studies demonstrate that it is possible to transplant a large number of discontinuous residues between DENV serotypes and partially recreate a complex antibody epitope, while retaining virus viability. Further refinement of this approach may lead to new tools for measuring epitope-specific antibody responses and new vaccine platforms.IMPORTANCEDengue virus is the most important mosquito-borne pathogen of humans worldwide, with approximately one-half the world's population living in regions where dengue is endemic. Dengue immunity following infection is robust and thought to be conferred by antibodies raised against the infecting virus. However, the specific viral components that these antibodies recognize and how they neutralize the virus have been incompletely described. Here we map a region on dengue virus serotype 3 recognized by the human neutralizing antibody 5J7 and then test the functional significance of this region by transplanting it into a serotype 1 virus. Our studies demonstrate a region on dengue virus necessary for 5J7 binding and neutralization. Our work also demonstrates the technical feasibility of engineering dengue viruses to display targets of protective antibodies. This technology can be used to develop new dengue vaccines and diagnostic assays.

scholarly journals Climatological, virological and sociological drivers of current and projected dengue fever outbreak dynamics in Sri Lanka

2020 ◽  
Vol 17 (167) ◽  
pp. 20200075 ◽  
Author(s):  
Caroline E. Wagner ◽  
Milad Hooshyar ◽  
Rachel E. Baker ◽  
Wenchang Yang ◽  
Nimalan Arinaminpathy ◽  
...  
Keyword(s):  
Sri Lanka ◽  
Dengue Virus ◽  
Disease Transmission ◽  
Temporal Dynamics ◽  
Sir Model ◽  
Extreme Weather Events ◽  
Climate Projections ◽  
Vector Borne Disease ◽  
Virus Serotype ◽  
Vector Borne

The largest ever Sri Lankan dengue outbreak of 2017 provides an opportunity for investigating the relative contributions of climatological, epidemiological and sociological drivers on the epidemic patterns of this clinically important vector-borne disease. To do so, we develop a climatologically driven disease transmission framework for dengue virus using spatially resolved temperature and precipitation data as well as the time-series susceptible-infected-recovered (SIR) model. From this framework, we first demonstrate that the distinct climatological patterns encountered across the island play an important role in establishing the typical yearly temporal dynamics of dengue, but alone are unable to account for the epidemic case numbers observed in Sri Lanka during 2017. Using a simplified two-strain SIR model, we demonstrate that the re-introduction of a dengue virus serotype that had been largely absent from the island in previous years may have played an important role in driving the epidemic, and provide a discussion of the possible roles for extreme weather events and human mobility patterns on the outbreak dynamics. Lastly, we provide estimates for the future burden of dengue across Sri Lanka using the Coupled Model Intercomparison Phase 5 climate projections. Critically, we demonstrate that climatological and serological factors can act synergistically to yield greater projected case numbers than would be expected from the presence of a single driver alone. Altogether, this work provides a holistic framework for teasing apart and analysing the various complex drivers of vector-borne disease outbreak dynamics.

scholarly journals Frequency of Dengue Virus Serotype 1 in Lahore by In-house assay

2020 ◽  
pp. 1-7
Author(s):  
Hajra Farooq ◽  
Waheed Uz Zaman Tariq ◽  
Sadia Bano ◽  
Ali Raza ◽  
Omar Rasheed Chughtai ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Dengue Virus ◽  
Real Time Pcr ◽  
Fast Track ◽  
Virus Rna ◽  
Virus Serotype ◽  
Serotype 1 ◽  
Dengue Virus Serotype 1 ◽  
Commercial Kit ◽  
Dengue Epidemic

Abstract Dengue is an important systemic viral infection that is caused by the dengue virus. Ribonucleic acid (RNA) from dengue NS1 positive samples, collected randomly during dengue epidemic from October 2016 to October 2017 at Chugtai Lab, was extracted for nucleic acid. Both the detection and serotyping of dengue samples were performed using real-time PCR on Rotor Gene Q. From the 70 NS1 positive samples, 57 (81.4%) samples were confirmed to be positive for dengue virus RNA, while the remaining 13 (18.6%) were negative. Serotype 1 (DEN-1) were verified among all samples by in-house assay and using commercial kit FTD (Fast Track Diagnostics) dengue differentiation; it was concluded that our in-house assay is in 100% concordance with commercial kit. Serotype 2 (DEN-2) and serotype 3 (DEN-3) have been documented in Pakistan since 1994. Continuous...  

scholarly journals Dengue Virus Serotype 2 (DEN-2): the Causative Agent of 2011-Dengue Epidemic in Pakistan

2012 ◽  
pp. 307-315 ◽  
Author(s):  
Muhammad Idrees ◽  
Waqar Hussain ◽  
Habib ur Rehman ◽  
Ghias un Nabbi Tayyab ◽  
Samia Afzal ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Causative Agent ◽  
Virus Serotype ◽  
Dengue Virus Serotype 2 ◽  
Dengue Epidemic

scholarly journals Molecular characterization and genotype shift of dengue virus strains between 2001 and 2014 in Guangzhou

2016 ◽  
Vol 145 (4) ◽  
pp. 760-765 ◽  
Author(s):  
L.Y. JIANG ◽  
Q. L. JING ◽  
Y. LIU ◽  
Y. M. CAO ◽  
W. Z. SU ◽  
...  
Keyword(s):  
High Risk ◽  
Dengue Virus ◽  
Nucleotide Substitution ◽  
Genotype I ◽  
Virus Serotype ◽  
Serotype 1 ◽  
The Mean ◽  
Different Origins ◽  
Virus Strains ◽  
Dengue Virus Serotype 1

SUMMARYWe studied the evolution, genotypes, and the molecular clock of dengue virus serotype 1 (DENV-1), between 2001 and 2014 in Guangzhou, China. The analysis of the envelope (E) gene sequences of 67 DENV-1 strains isolated in Guangzhou, together with 58 representative sequences downloaded from NCBI, have shown shifts in viral genotypes. The genotype changed several times, from genotype I to IV in 2002, from IV to I in 2005, and from I to V in 2014. These genotype shifts may be the cause of DENV outbreaks. The diversity of genotypes and clades demonstrates a high risk of future outbreaks in Guangzhou. The mean rate of virus nucleotide substitution in Guangzhou was determined to be 7·77 × 10−4 per site per year, which represents a medium substitution rate compared to two other countries. Our research can point to different ancestors of the isolated strains, which may further reveal the different origins and transmission of DENV-1 strains in Guangzhou.

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