scholarly journals Recent expansion of dengue virus serotype 3 in West Africa

2010 ◽  
Vol 15 (7) ◽  
Author(s):  
L Franco ◽  
A Di Caro ◽  
F Carletti ◽  
O Vapalahti ◽  
C Renaudat ◽  
...  
Keyword(s):  
West Africa ◽  
Dengue Virus ◽  
Cape Verde ◽  
The Past ◽  
Virus Serotype ◽  
Dengue Epidemic

Due to non-existing or limited surveillance in Africa, little is known about the epidemiology of dengue illness in the continent. Serological and virological data obtained from returning European travellers is a key complement to this often flawed information. In the past years, dengue 3 virus has emerged in West Africa and has been detected in travellers returning to Europe. The first dengue epidemic in Cape Verde with more than 17,000 cases from September to December 2009 demonstrated that dengue virus is still expanding worldwide to new territories.

scholarly journals Dengue Virus Serotype 3 Infection in Traveler Returning from West Africa to Germany

2015 ◽  
Vol 21 (1) ◽  
pp. 175-177 ◽  
Author(s):  
Isabella Eckerle ◽  
Annette Kapaun ◽  
Thomas Junghanss ◽  
Paul Schnitzler ◽  
Christian Drosten ◽  
...  
Keyword(s):  
West Africa ◽  
Dengue Virus ◽  
Virus Serotype

scholarly journals Emergence of genotype I of dengue virus serotype 3 during severe dengue epidemic in Sri Lanka, 2017

2021 ◽  
Author(s):  
Mya Myat Ngwe Tun ◽  
Rohitha Muthugala ◽  
Lakmali Rajamanthri ◽  
Takeshi Nabeshima ◽  
Corazon C. Buerano ◽  
...  
Keyword(s):  
Sri Lanka ◽  
Dengue Virus ◽  
Severe Dengue ◽  
Genotype I ◽  
Virus Serotype ◽  
Dengue Epidemic

scholarly journals Frequency of Dengue Virus Serotype 1 in Lahore by In-house assay

2020 ◽  
pp. 1-7
Author(s):  
Hajra Farooq ◽  
Waheed Uz Zaman Tariq ◽  
Sadia Bano ◽  
Ali Raza ◽  
Omar Rasheed Chughtai ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Dengue Virus ◽  
Real Time Pcr ◽  
Fast Track ◽  
Virus Rna ◽  
Virus Serotype ◽  
Serotype 1 ◽  
Dengue Virus Serotype 1 ◽  
Commercial Kit ◽  
Dengue Epidemic

Abstract Dengue is an important systemic viral infection that is caused by the dengue virus. Ribonucleic acid (RNA) from dengue NS1 positive samples, collected randomly during dengue epidemic from October 2016 to October 2017 at Chugtai Lab, was extracted for nucleic acid. Both the detection and serotyping of dengue samples were performed using real-time PCR on Rotor Gene Q. From the 70 NS1 positive samples, 57 (81.4%) samples were confirmed to be positive for dengue virus RNA, while the remaining 13 (18.6%) were negative. Serotype 1 (DEN-1) were verified among all samples by in-house assay and using commercial kit FTD (Fast Track Diagnostics) dengue differentiation; it was concluded that our in-house assay is in 100% concordance with commercial kit. Serotype 2 (DEN-2) and serotype 3 (DEN-3) have been documented in Pakistan since 1994. Continuous...  

scholarly journals Emergence of dengue virus serotype 2 in Mauritania and molecular characterization of its circulation in West Africa

2021 ◽  
Vol 15 (10) ◽  
pp. e0009829
Author(s):  
Toscane Fourié ◽  
Ahmed El Bara ◽  
Audrey Dubot-Pérès ◽  
Gilda Grard ◽  
Sébastien Briolant ◽  
...  
Keyword(s):  
West Africa ◽  
Dengue Virus ◽  
Dengue Fever ◽  
West African ◽  
Capital City ◽  
Epidemic Strain ◽  
African Countries ◽  
Recent Emergence ◽  
Virus Serotype ◽  
Dengue Virus Serotype 2

The number of sporadic and epidemic dengue fever cases have reportedly been increasing in recent years in some West African countries, such as Senegal and Mali. The first epidemic of laboratory-confirmed dengue occurred in Nouakchott, the capital city of Mauritania situated in the Saharan desert, in 2014. On-site diagnosis of dengue fever was established using a rapid diagnostic test for dengue. In parallel, the presence of Aedes aegypti mosquitoes in the city was confirmed. The initial diagnosis was confirmed by RT-PCR, which showed that all samples from the 2014 dengue epidemic in Nouakchott were dengue virus serotype 2 (DENV-2). The whole genome or envelope protein gene of these strains, together with other DENV-2 strains obtained from travelers returning from West African countries to France between 2016 and 2019 (including two Mauritanian strains in 2017 and 2018), were sequenced. Phylogenetic analysis suggested a recent emergence of an epidemic strain from the cosmopolitan genotype belonging to West African cosmopolitan lineage II, which is genetically distinct from African sylvatic genotype. The origin of this DENV-2 lineage is still unknown, but our data seem to suggest a recent and rapid dispersion of the epidemic strain throughout the region. More complete genome sequences of West African DENV-2 are required for a better understanding of the dynamics of its circulation. Arboviral surveillance and outbreak forecasting are urgently needed in West Africa.

scholarly journals Dengue Virus Serotype 2 (DEN-2): the Causative Agent of 2011-Dengue Epidemic in Pakistan

2012 ◽  
pp. 307-315 ◽  
Author(s):  
Muhammad Idrees ◽  
Waqar Hussain ◽  
Habib ur Rehman ◽  
Ghias un Nabbi Tayyab ◽  
Samia Afzal ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Causative Agent ◽  
Virus Serotype ◽  
Dengue Virus Serotype 2 ◽  
Dengue Epidemic

scholarly journals Emergence And Circulation of Dengue Virus Serotype 2 In Guangzhou Over A Period of 20 Years

2021 ◽  
Author(s):  
Liyun Jiang ◽  
Yuan Liu ◽  
Wenzhe Su ◽  
Yimin Cao ◽  
Qinlong Jing ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Indian Subcontinent ◽  
Envelope Gene ◽  
Maximum Clade Credibility ◽  
Health Infrastructure ◽  
Virus Serotype ◽  
Imported Cases ◽  
Dengue Virus Serotype 2 ◽  
Dengue Epidemic ◽  
Pressure Analysis

Abstract Objectives The dengue epidemic in Guangzhou has imposed a rising burden on society and health infrastructure. Here we present the genotype data for dengue virus serotype 2 (DENV-2) to improve the understanding of dengue epidemic. Methods We sequenced the envelope gene of DENV-2 obtained from patient serum sample, and subsequently performed the maximum-likelihood phylogenetic analysis using PhyMLv3.1, the maximum clade credibility analysis using BEAST v.1.10.4 and selection pressure analysis using Datamonkey 2.0 . Results The DENV-2 prevalent in Guangzhou region related to the strains of Southeast Asian countries.Our results suggest that the Malaysia/Indian subcontinent genotype is prevalent in Guangzhou and no genotype shift has occurred during the last 20 years. Episodic positive selection was detected at one site. Conclusions Prevention and monitoring imported cases are important for local control. The shift between the lineages of the Malaysia/Indian subcontinent genotype, which originated at different time points, may be the underlying cause of rising DENV-2 cases in Guangzhou.The low rate of dengue haemorrhagic fever in Guangzhou may be explained by the dominance of the less virulent Malaysia/Indian subcontinent genotype.

scholarly journals Genomic investigation of a dengue virus outbreak in Thiès, Senegal, in 2018

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Amy Gaye ◽  
Tolla Ndiaye ◽  
Mouhamad Sy ◽  
Awa B. Deme ◽  
Alphonse B. Thiaw ◽  
...  
Keyword(s):  
West Africa ◽  
Dengue Virus ◽  
Clinical Signs ◽  
Health Concern ◽  
Public Health Concern ◽  
Local Circulation ◽  
Viral Genomes ◽  
Genomic Characterization ◽  
The City

AbstractDengue virus is a major and rapidly growing public health concern in tropic and subtropic regions across the globe. In late 2018, Senegal experienced its largest dengue virus outbreak to date, covering several regions. However, little is known about the genetic diversity of dengue virus (DENV) in Senegal. Here we report complete viral genomes from 17 previously undetected DENV cases from the city of Thiès. In total we identified 19 cases of DENV in a cohort of 198 individuals with fever collected in October and November 2018. We detected 3 co-circulating serotypes; DENV 3 was the most frequent accounting for 11/17 sequences (65%), 4 (23%) were DENV2 and 2 (12%) were DENV1. Sequences were most similar to recent sequences from West Africa, suggesting ongoing local circulation of viral populations; however, detailed inference is limited by the scarcity of available genomic data. We did not find clear associations with reported clinical signs or symptoms, highlighting the importance of testing for diagnosing febrile diseases. Overall, these findings expand the known range of DENV in Senegal, and underscore the need for better genomic characterization of DENV in West Africa.

scholarly journals Micafungin Inhibits Dengue Virus Infection through the Disruption of Virus Binding, Entry, and Stability

2021 ◽  
Vol 14 (4) ◽  
pp. 338
Author(s):  
Yen-Chen Chen ◽  
Jeng-Wei Lu ◽  
Chia-Tsui Yeh ◽  
Te-Yu Lin ◽  
Feng-Cheng Liu ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Dengue Fever ◽  
Early Stage ◽  
Quantitative Polymerase Chain Reaction ◽  
Enterovirus 71 ◽  
Denv Infection ◽  
Immunofluorescence Assay ◽  
Dependent Manner ◽  
Virus Binding ◽  
Virus Serotype

Dengue fever is an arbovirus disease caused by infection with the dengue virus (DENV). Half of the world’s population lives under the threat of dengue fever, however, researchers have yet to develop any drugs that are clinically applicable to this infection. Micafungin is a member of the echinocandins family of anti-fungal drugs, capable of blocking the synthesis of β-1,3-D-glucan in the walls of fungal cells. Previous studies have demonstrated the effectiveness of Micafungin against infections of enterovirus 71 (EV71) and chikungunya virus (CHIKV). This is the first study demonstrating the effectiveness of micafungin in inhibiting the cytopathic effects of dengue virus serotype 2 (DENV-2) in a dose-dependent manner. Time-of-addition assays verified the inhibitory effects of micafungin in pre-treated, co-treated, and full-treatment groups. Binding and entry assays also demonstrated the effectiveness of micafungin in the early stage of DENV-2 infection. The virucidal efficacy of micafungin appears to lie in its ability to destroy the virion. Molecular docking assays revealed the binding of micafungin to the envelope protein of DENV-2, thereby revealing the mechanism by which micafungin affects the early stage of DENV infection and the stability of DENV. Two other micafungin analogs, caspofungin and anidulafungin, were also shown to have the antiviral effects on DENV-2. Finally, immunofluorescence assay (IFA) and reverse-transcription quantitative polymerase chain reaction (RT-qPCR) confirmed the broad anti-DENV ability of micafungin against dengue virus serotypes 1, 3, and 4 (DENV-1, DENV-3, and DENV-4). Taken together, these results demonstrate the potential of micafungin and its analogs as candidates for the development of broad-spectrum treatments for DENV infection.

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