scholarly journals Green Synthesis, Characterization and In-vitro Antioxidant Property of Silver Nanoparticles Using the Aqueous Leaf Extract of Justicia carnea

2021 ◽  
pp. 20-30
Author(s):  
I. O. Salaudeen ◽  
M. O. Olajuwon ◽  
A. B. Ajala ◽  
T. O. Abdulkareem ◽  
S. A. Adeniyi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Silver Ions ◽  
Antioxidant Property ◽  
Biological Macromolecules ◽  
Aqueous Leaf Extract ◽  
In Vitro Antioxidant Activity

This study investigated the synthesis, characterization and in vitro antioxidant activity of silver nanoparticles (AgNPs) using the aqueous leaf extract of Justicia carnea.  The aqueous leaf extract of J. carnea was used as a potential reducing and capping agent. To identify the compounds responsible for the reduction of silver ions, the functional groups present in the plant extract were subjected to FTIR. The in vitro antioxidant activity of synthesized nanoparticles was evaluated in terms of ferric reducing antioxidant potential (FRAP), DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2`-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) free radicals scavenging assays. The surface plasmon resonance confirmed the formation of AgNPs with maximum absorbance at kmax = 446 nm. FTIR revealed the biological macromolecules of J. carnea leaf extract involved in the synthesis and stabilization of AgNPs. UV-Visible spectrophotometer showed absorbance peak in the range of 436-446 nm. The silver nanoparticles exhibited moderate antioxidant activities compared to standard antioxidants (ascorbic acid and BHT). These results confirmed this protocol as simple, eco-friendly, nontoxic and an alternative for conventional physical and chemical methods. It can be concluded that J. carnea leaf extract can be used effectively in the production of potential antioxidant AgNPs which could be useful in various bio-applications such as cosmetics, food and biomedical industry.

Analysing the phytochemistry and anti-oxidant property of fabricated silver nanoparticles using Catharanthus roseus leaf extract

2021 ◽  
Vol 16 (12) ◽  
pp. 72-79
Author(s):  
A.K. Keshari ◽  
S. Saxena ◽  
G. Pal ◽  
V. Srivashtav ◽  
R. Srivastav
Keyword(s):  
Silver Nanoparticles ◽  
Electron Microscope ◽  
Catharanthus Roseus ◽  
Leaf Extract ◽  
Spherical Shape ◽  
Silver Ions ◽  
Antioxidant Property ◽  
X Ray ◽  
Morphological Studies ◽  
Aqueous Leaf Extract

A green rapid biogenic synthesis of silver nanoparticles (AgNPs) using Catharanthus roseus leaf extract was performed. Synthesized nanoparticles were characterized using UV-Visible, Fourier transform infra-red (FTIR) and X-ray diffraction (XRD). The reduction of silver ions to AgNPs using C. roseus extract was completed within 240 mins. The formation of AgNPs was confirmed by Surface Plasmon Resonance (SPR) at 442 nm using UV-Vis Spectrophotometer and it is characterized by XRD, Transmission electron microscope (TEM) and Scanning electron microscope (SEM). The morphological studies revealed the spherical shape of the particles with sizes ranging from 16-35 nm and Energy dispersive X-ray (EDX) spectrum confirmed the presence of silver along with other elements in the plants metabolite. The extracellular AgNPs synthesis by aqueous leaf extract demonstrates ultra-fast, simple and inexpensive method comparable to other methods. The antioxidant assay of the synthesized AgNPs indicated that they have a strong antioxidant property as compared to the control. Since these compounds are also safe to use and discharged into the environment, the green AgNPs could be considered as an innovative alternative approach for biomedical and nanoscience based industries.

scholarly journals In Vitro Antioxidant Activity of Hydrophila auriculata Leaves Extract and Silver Nanoparticles

2018 ◽  
Vol 4 (5) ◽  
pp. 549-551 ◽  
Author(s):  
M. Ravishankar ◽  
V. Christibai Juliet Esther
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Elemental Silver ◽  
Total Antioxidant ◽  
Dose Dependent ◽  
In Vitro Antioxidant Activity

Antioxidant activity of Hydrophila auriculata leaf extract and silver nanoparticles (AgNPs) were carried out for proving its utility in free radical mediated diseases. The flower extract and silver nanoparticles were screened for in vitro antioxidant activity by oxygen radical scavenging such as DPPH, total antioxidant assay and superoxide radical scavenging activity at different concentrations. The antioxidant potential was found to be dose dependent. AgNPs possess probable antioxidant activity as compared with Hydrophila auriculata leaf extract and near to the standard. It is an obvious indication that the resulting activity of the AgNPs is not due to any capping agents, which are found in minute amounts but is due to the elemental silver.

ASSESSMENT OF PHYTOCHEMICALS, TOTAL PHENOL, FLAVONOID CONTENT AND IN VITRO ANTIOXIDANT PROPERTY OF LARGE CARDAMOM EXTRACTS

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (10) ◽  
pp. 34-41
Author(s):  
Kanthlal S. K. ◽  
Jipnomon Joseph ◽  
Bindhu P. Paul ◽  
Vijayakumar M ◽  
Rema Shree A. B. ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Property ◽  
Bioactive Constituents ◽  
Preliminary Assessment ◽  
Reducing Power Assay ◽  
Large Cardamom

Amomum subulatum, commonly known as large or black cardamom, is a commonly used spice in Indian kitchens and is traditionally used to treat various ailments. To add more knowledge about the medicinal values of the fruit, this study was conducted to evaluate the in vitro antioxidant activities of aqueous, methanol, ethanol, hydro alcohol, ethyl acetate, acetone and chloroform extracts of the fruit. Preliminary assessment was done to detect the presence of phytoconstituents using identification tests. The antioxidant activity was measured by employing methods such as diphenylpicrylhydrazyl (DPPH) radical scavenging assay, total antioxidant activity equivalent to ascorbic acid, reducing power assay and superoxide anion scavenging assay. The antioxidant activities were compared with their respective phenol and flavonoid contents. Preliminary assessment revealed that large cardamom fruit is a good source of all the bioactive constituents as well as phenol and flavonoid essential for medicinal values. The extract obtained by polar solvents showed the highest antioxidant efficacy in relation to its phenol content. Also, all the solvent-soluble fractions showed a concentration-dependent antioxidant effect. Results from our study prove that large cardamom can alleviate oxidative stress, suggesting the potential of large cardamom as a functional food

scholarly journals Assessment of in vitro Antioxidant and Antidiabetic Capacities of Medlar (Mespilus germanica)

2018 ◽  
Vol 47 (2) ◽  
pp. 384-389
Author(s):  
Sebnem Selen ISBILIR ◽  
Sevilay Inal KABALA ◽  
Hulya YAGAR
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Inhibitory Effect ◽  
Total Phenolic ◽  
Flower Bud ◽  
Ethanol Extracts ◽  
Dpph Scavenging ◽  
Β Carotene

The objective of the current study was to evaluate the antioxidant activity and enzyme inhibitory effect of different parts of medlar including fruit, leaf and flower bud by using various in vitro methods, and also determination of total phenolic and flavonoid content in the samples. Ethanol extracts of medlar parts were prepared and their antioxidant activities were determined using 1,1-diphenyl-2-picryl-hydrazil (DPPH•) scavenging and β-carotene bleaching methods. The leaf extract showed the strongest antioxidant activity. DPPHradical scavenging activity was in the order of BHA > leaf > bud > fruit. This ordering was the same for β-carotene bleaching activity, tocopherol > leaf > bud > fruit. The highest total phenolic (60.3 ± 1.69 mg GAE g-1 extract) and flavonoid (14.77 ± 1.15 mg QE g-1 extract) content were determined in leaf extract. For possible antidiabetic effects of extracts, α-amylase and α-glucosidase inhibitory activities were investigated, the bud extract showed the highest inhibition activities among the all extracts.

scholarly journals Degradation of Methylene Blue Using Biologically Synthesized Silver Nanoparticles

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
M. Vanaja ◽  
K. Paulkumar ◽  
M. Baburaja ◽  
S. Rajeshkumar ◽  
G. Gnanajobitha ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Nitrate Solution ◽  
Reaction Medium ◽  
Silver Ions ◽  
Silver Nitrate Solution ◽  
X Ray ◽  
Synthesis Of Nanoparticles ◽  
Aqueous Leaf Extract

Nowadays plant mediated synthesis of nanoparticles has great interest and achievement due to its eco-benign and low time consuming properties. In this study silver nanoparticles were successfully synthesized by usingMorinda tinctorialeaf extract under different pH. The aqueous leaf extract was added to silver nitrate solution; the color of the reaction medium was changed from pale yellow to brown and that indicates reduction of silver ions to silver nanoparticles. Thus synthesized silver nanoparticles were characterized by UV-Vis spectrophotometer. Dispersity and morphology was characterized by scanning electron microscope (SEM); crystalline nature and purity of synthesized silver nanoparticles were revealed by X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDX). FTIR spectrum was examined to identify the effective functional molecules responsible for the reduction and stabilization of silver nanoparticles synthesized by leaf extract. The photocatalytic activity of the synthesized silver nanoparticles was examined by degradation of methylene blue under sunlight irradiation. Green synthesized silver nanoparticles were effectively degrading the dye nearly 95% at 72 h of exposure time.

One-pot construction of sterically challenging and diverse polyarylphenols via transition-metal-free benzannulation and their potent in vitro antioxidant activity

2017 ◽  
Vol 15 (9) ◽  
pp. 2052-2062 ◽  
Author(s):  
Shizuka Mei Bautista Maezono ◽  
Tej Narayan Poudel ◽  
Yong Rok Lee
Keyword(s):  
Antioxidant Activity ◽  
Transition Metal ◽  
Antioxidant Activities ◽  
One Pot ◽  
Metal Free ◽  
Novel Synthesis ◽  
In Vitro Antioxidant Activity

One pot novel synthesis of highly functionalized polyarylphenols via benzannulation under transition-metal-free conditions and their antioxidant activities are described.

scholarly journals In-vitro and In-vivo Antioxidant Activity of Ethanol Leaf Extract of Justicia carnea

2020 ◽  
pp. 48-60
Author(s):  
Udedi Stanley Chidi ◽  
Ani Onuabuchi Nnenna ◽  
Asogwa Kingsley Kelechi ◽  
Maduji Fitzcharles Chijindu ◽  
Okafor Clinton Nebolisa
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Superoxide Dismutase ◽  
Leaf Extract ◽  
Dpph Radical ◽  
Β Carotene ◽  
In Vitro Antioxidant Activity

This study investigated the in-vitro antioxidant activity of ethanol leaf extract of Justicia carnea and its effect on antioxidant status of alloxan-induced diabetic albino rats. The in-vitro antioxidant activity was assayed by determining the total phenol, flavonoids, ascorbic acid, β-carotene and lycopene contents and by using 2,2 diphenyl-1-picrylhydrazyl (DPPH) radical, reducing antioxidant power and inhibition of lipid peroxidation antioxidant systems. Oxidative stress was produced in rats by single intraperitoneal injection of 150 mg/kg alloxan and serum concentration of malonaldehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) were determined. Five experimental groups of rats (n=6) were used for the study. Two groups of diabetic rats received oral daily doses of 100 and 200 mg/kg Justicia carnea leaf extract respectively while gilbenclamide (5 mg/ml); a standard diabetic drug was also given to a specific group for 14 days. From the result, the leaf extract contained a higher concentration of flavonoids followed byphenols, ascorbic acid, lycopene and β-carotene. The extract displayed more potent reducing power ability with EC50 of 40 µg/ml compared to BHA (EC50 of 400µg/ml). The percentage DPPH radical scavenging activity of the extract was also higher with EC50 of 200µg/ml and increased with increase in concentration while BHA had EC50of 320µg/ml. The inhibition of lipid peroxidation also increased with increase in concentration with EC50 of 58µg/ml and comparable with BHA (EC50=60µg/ml). The effect of the plant extract on antioxidant enzyme activities was concentration-dependent. Administration of 100mg/kg of the plant extract resulted in a significant decrease (p<0.05) in serum MDA concentration, while 200 mg/kg of the extract caused a significant (p˂0.05) increase in superoxide dismutase (SOD) and catalase activities with a non-significant increase (p>0.05) in the serum level of MDA when compared with the diabetic untreated group. These findings suggest that ethanol leaf extract of Justicia carnea have antioxidant properties and could handle diabetes-induced oxidative stress.

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