scholarly journals Assessment of in vitro Antioxidant and Antidiabetic Capacities of Medlar (Mespilus germanica)

2018 ◽  
Vol 47 (2) ◽  
pp. 384-389
Author(s):  
Sebnem Selen ISBILIR ◽  
Sevilay Inal KABALA ◽  
Hulya YAGAR
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Inhibitory Effect ◽  
Total Phenolic ◽  
Flower Bud ◽  
Ethanol Extracts ◽  
Dpph Scavenging ◽  
Β Carotene

The objective of the current study was to evaluate the antioxidant activity and enzyme inhibitory effect of different parts of medlar including fruit, leaf and flower bud by using various in vitro methods, and also determination of total phenolic and flavonoid content in the samples. Ethanol extracts of medlar parts were prepared and their antioxidant activities were determined using 1,1-diphenyl-2-picryl-hydrazil (DPPH•) scavenging and β-carotene bleaching methods. The leaf extract showed the strongest antioxidant activity. DPPHradical scavenging activity was in the order of BHA > leaf > bud > fruit. This ordering was the same for β-carotene bleaching activity, tocopherol > leaf > bud > fruit. The highest total phenolic (60.3 ± 1.69 mg GAE g-1 extract) and flavonoid (14.77 ± 1.15 mg QE g-1 extract) content were determined in leaf extract. For possible antidiabetic effects of extracts, α-amylase and α-glucosidase inhibitory activities were investigated, the bud extract showed the highest inhibition activities among the all extracts.

scholarly journals Ekstrak Etanol Daun Belimbing Wuluh (Averrhoa bilimbi) sebagai Antioksidan dan Antiinflamasi

2019 ◽  
Vol 8 (3) ◽  
pp. 86
Author(s):  
Hasim Hasim ◽  
Yupi Yulianita Arifin ◽  
Dimas Andrianto ◽  
Didah Nur Faridah
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Phytochemical Compounds ◽  
Anti Inflammatory ◽  
Ethanol Extracts ◽  
Phenolic And Flavonoid

Belimbing wuluh merupakan tanaman jenis buah dan obat tradisional. Tanaman belimbing wuluh sudah sering dimanfaatkan masyarakat salah satunya untuk mengobati penyakit seperti batuk dan radang rektum. Tujuan penelitian ini adalah menguji senyawa fitokimia, menganalisis kandungan total fenolik dan flavonoid, serta aktivitas antioksidan dan antiinflamasi secara in vitro pada ekstrak etanol daun belimbing wuluh. Metode yang dilakukan pada penelitian ini adalah ekstraksi daun belimbing wuluh, skrining fitokimia, perhitungan total fenol dan flavonoid, uji aktivitas antioksidan, dan uji aktivitas antiinflamasi. Senyawa fitokimia yang terkandung pada ekstrak etanol daun belimbing wuluh adalah saponin, tanin, steroid, flavonoid, dan alkaloid. Kandungan total fenolik dan flavonoid ekstrak etanol daun belimbing wuluh secara berturut-turut sebesar 39,03 dan 97,28 µg QE/mg. Ekstrak etanol daun belimbing wuluh memiliki aktivitas antioksidan yang tergolong sangat kuat, sementara aktivitas antiinflamasinya terutama ditunjukkan pada konsentrasi ekstrak 200 µg/ ml, memiliki nilai persen inhibisi hemolisis yang paling tinggi. Kesimpulannya, ekstrak etanol daun belimbing wuluh dapat menjadi salah satu sumber antioksidan dan antiinflamasi alami.Ethanol Extracts of Averrhoa Bilimbi Leaf Demonstrated Antioxidative and Anti-inflammatory ActivityAbstractAverrhoa bilimbi, a fruit-bearing plant, has been traditionally used for medicinal purposes such as treatment of cough and rectal inflammation. In this current work, ethanol extract of Averrhoa bilimbi leaf was analyzed, with regard to phytochemical composition, i.e. total phenolic, and flavonoid, antioxidant activity, and in vitro antiinflammation activity. Extraction of carambola leaves, phytochemical screening, total phenolic and flavonoid contens, assay of antioxidant activity, and assay of anti-inflammatory activity were done in this research. As a result, the leaf extract positively contained some phytochemical compounds, i.e. saponin, tannin, steroid, flavonoid, and alkaloid. Furthermore, total phenolic and flavonoid of the leaf extract was found at 39.03 and 97.28 µg QE/mg extract, respectively. Additionally, antioxidant activity of the leaf extract was classified as very strong, while its anti-inflammatory feature at extract concentration of 200 µg/ml exhibited the highest inhibition of hemolysis. In summary, the ability of Averrhoa bilimbi leaf ethanol extracts to act as antioxidative and anti-inflammatory agents was determined and this may open the use for natural antioxidant and anti-inflammatory agents.

Estimation of Total Phenolics and Flavonoid Content and In-vitro Antioxidant Activity of Drypetes sepiaria Stem (Wight and Arn.) Pax and K. Hoffm.

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Packia M. ◽  
Daffodil D. ◽  
Tresina S. ◽  
Mohan R.
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolics ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Model Systems ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Ethanol Extracts ◽  
In Vitro Antioxidant Activity

The total phenolics, flavonoids and in vitro antioxidant activity of petroleum ether, benzene, ethyl acetate, methanol and ethanol extracts of stem of Drypetes sepiaria were determined using various antioxidant model systems viz, DPPH, hydroxyl, superoxide, ABTS and reducing power. Total phenolic content was estimated by Folin-Ciocalteau method. Flavonoids were determined by Aluminium chloride method. The total phenolics and flavonoids contents were found to be 0.81 g 100 g-1 and 1.12g 100 g-1 respectively in the methanol extract. Among the solvent tested, methanol and ethanol extracts of D. sepiaria stem showed potent in vitro antioxidant activities. This investigation explored that D. sepiaria stem is a potential source of natural antioxidant.

Phenolic Content, Antibacterial and Antioxidant Activities of Moroccan propolis

2020 ◽  
Vol 15 (6) ◽  
pp. 696-705
Author(s):  
Omar Belmehdi ◽  
Badia Douhri ◽  
Abdelhakim Bouyahya ◽  
Yousif Laghmouchi ◽  
Nadia S. Senhaji ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Capacity ◽  
Bleaching Test ◽  
Ethanol Extracts ◽  
Dpph Scavenging

Background:Propolis contains compounds with antibacterial and antioxidant capacities, such as phenolic compounds and flavonoids. The aim of this study was to investigate the antibacterial and antioxidant effects of six propolis samples from northern Morocco and to determine their content of polyphenols and flavonoids.Methods:The total polyphenols and flavonoids of Ethanol Extracts (EEP) were evaluated by colorimetric assays. To test the antibacterial activity, the well diffusion and micro-dilution methods were adopted. The kinetic of bacterial growth in the presence of EEP was tested. The antioxidant activity was evaluated using three techniques: reducing power, β-carotene bleaching test, and DPPH scavenging capacity assay.Results:The EEPs were found to have high total phenolic content (65.09 - 122.00 mg GAE/g) and flavonoids (41.95 - 50.44 mg QE/g). The screening of the antibacterial activity showed that all tested bacteria were sensitive to EEP except Escherichia coli. The inhibitory zones varied from 11.50 ± 0.70 to 32.00 ± 0.70 mm, and MIC and MBC ranged from 0.07 to 5 mg/mL and 0.07 to >5 mg/mL, respectively. The antioxidant activity was proportional to the phenolic content of propolis. The IC50 varied from 48.70 to 156.00 mg/mL and the RAA% ranged from 75.07 to 96.68 %.

scholarly journals In-Vitro Antioxidant Activity and Total Phenolic Content of Ruta montana L. Extracts

2020 ◽  
Vol 10 (2) ◽  
pp. 69-75
Author(s):  
Mounira Merghem ◽  
Saliha Dahamna
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Chloroform Extract ◽  
Total Phenolic ◽  
Ruta Montana ◽  
Β Carotene

The aim of this study is to evaluate in vitro antioxidant activities of Ruta montana L.  extracts. This activity was evaluated by three methods : DPPH (2, 2'-diphenyl- 1- picrylhydrazy), bleaching of β-carotene and chelation of ferrous iron. Results showed that ethyl acetate extract (EAE) represents the highest amount of total polyphenols, tannins and flavonoids with 257,1 ± 0,703µg gallic acid equivalent/mg of extract,  251 ± 1.41 µg tannic acid equivalent /mg of extract,117,4 ± 3,451 µg quercetin equivalents/mg of extract, 139,5 ± 4,107 µg rutin equivalents/mg of extract, respectively. In the DPPH assay, ethyl acetate extract showed the higher scavenging capacity (IC50 = 0.044 ± 0.001 mg/ml) followed by methanol, aqueous and chloroform extract. Whereas, AqE showed the best chelating effect and the best inhibitory capacity of the coupled oxidation of linoleic acid/ β-carotene. Keywords: Ruta montana L; polyphenols; antioxidant activity; free radical scavenging.

In vitro antioxidant and enzyme inhibitory properties and phenolic contents of crude extracts and fractions from different organs of the halophyte Lycium europaeum L.

2021 ◽  
Vol 17 ◽  
Author(s):  
Houaria Bendjedou ◽  
Malika Bennaceur ◽  
Houari Benamar ◽  
Maria João Rodrigues ◽  
Catarina Pereira ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Biological Properties ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Ic50 Values ◽  
Reducing Activity ◽  
Ethanol Extracts ◽  
High Level ◽  
Β Carotene

Background: Lycium europaeum L. is a medicinal and edible Mediterranean halophyte spiny shrub. However, studies regarding its biological properties focused mainly on its aerial organs. Objective: The objective of the present work was to make a comparative evaluation of the in vitro antioxidant and inhibitory enzyme activities of ethanol extracts and fractions (chloroform, ethyl acetate, n-butanol and aqueous) from roots and leaves of L. europaeum, along with its total phenolic, flavonoid and tannin contents. Methods: The antioxidant activity was evaluated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) 2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), superoxide radical, β-carotene bleaching, cupric reducing, and the ferric reducing activity methods. Results: The n-butanol fraction from roots had the highest antioxidant activity in all the assays. It was also the most active against acetylcholinesterase, butyrylcholinesterase, and urease (IC50 values 92.63, 118.26, and 135.60 µgmL-1, respectively). This fraction showed a high level of total phenolic, flavonoid, and tannin contents. Conclusion: The results suggest L. europaeum, especially its roots, as a candidate to be further explored as a source of bioactive products.

scholarly journals In vitro antioxidant activity of stem bark of Trichilia catigua Adr. Juss

2012 ◽  
Vol 62 (3) ◽  
pp. 371-382 ◽  
Author(s):  
Jean Paul Kamdem ◽  
Sílvio Terra Stefanello ◽  
Aline Augusti Boligon ◽  
Caroline Wagner ◽  
Ige Joseph Kade ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Stem Bark ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
High Concentrations ◽  
Dpph Scavenging

Antioxidant activity of the ethanolic extract and fractions from the stem bark of T. catigua was investigated. IC50 (for DPPH scavenging) by T. catigua varied from 9.17 ± 0.63 to 76.42 ± 5.87 mg mL-1 and total phenolic content varied from 345.63 ± 41.08 to 601.27 ± 42.59 mg GAE g-1 of dry extract. Fe2+-induced lipid peroxidation was significantly reduced by the ethanolic extract and fractions. Mitochondrial Ca2+-induced dichlorofluorescein oxidation was significantly reduced by the ethanolic extract in a concentration-dependent manner. Ethanolic extract reduced mitochondrial Dym only at high concentrations (40-100 mg mL-1), which indicates that its toxicity does not overlap with its antioxidant effects. Results suggest involvement of antioxidant activities of T. catigua in its pharmacological properties.

scholarly journals HPLC-UV profile of Genista ulicina Spach. (Fabaceae) extracts and in vitro antioxidant activity

2016 ◽  
Vol 8 (3) ◽  
pp. 398 ◽  
Author(s):  
Kaoutar Chebbah ◽  
Eric Marchioni ◽  
Djamel Sarri ◽  
Ahmed Menad ◽  
Ratiba Mekkiou ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Colorimetric Method ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Uv Detector ◽  
Etoac Extract ◽  
Phenolic And Flavonoid ◽  
Β Carotene

<p>To perform a qualitative and quantitative analysis of the phenolic and flavonoid contents and evaluate the antioxidant activity of ethyl acetate (EtOAc) and <em>n</em>-butanol (<em>n</em>-BuOH) extracts of the aerial parts of <em>Genista ulicina </em>Spach. from Algeria.<strong> </strong>The qualitative analysis of plant extracts was carried out by RP-HPLC using UV detector, whereas the quantification of total phenolic and flavonoid contents was completed according to the Folin-Ciocalteu procedure and aluminium chloride colorimetric method respectively. To evaluate the extract's antioxidant activity, Two in vitro antioxidant tests were employed: DPPH and β-carotene bleaching assay. The HPLC/DAD chromatogram showed several peaks indicating the presence of phenolic acids, flavonoids and isoflavonoids in both extracts. The total phenolic content (TPC) ranged from 62.56 and 50.45 mgGAE/g extract, while the total flavonoids content varied between 53.1 and 48.4 mgQE/g extract for EtOAC and <em>n</em>-BuOH respectively. EtOAc extract showed a maximum inhibition value (78.15%) at 150µg/mL using DPPH test and highest antioxidative power (82.42%) using β-carotene bleaching assay comparing with standards. The HPLC-UV analysis showed the richeness of both extracts in phenolic and flavonoid contents. The EtOAc<em> </em>extract exhibited good antioxidant activities comparing to the <em>n</em>-BuOH extract. Thus <em>Genista ulicina</em> could be indicated as a plant of phytopharmaceutical importance.<strong></strong></p>

Determination of Antioxidant Activity In Vitro from Hyptis rhomboidea Mart. et Gal. Ethanol Extracts

2014 ◽  
Vol 472 ◽  
pp. 824-828 ◽  
Author(s):  
Xi Feng Li ◽  
Lu Tang ◽  
Nan Nan Zheng ◽  
Shuo An ◽  
You Xu Wang ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Positive Control ◽  
Fresh Market ◽  
Ethanol Extracts ◽  
Dpph Scavenging ◽  
Selection Of

Antioxidants are the chemical substances that reduce or prevent oxidation. The present study aimed to assess in vitro antioxidant activities of ethanol extracts Hyptis rhomboidea. DPPH, ABTS and FRAP assays were used for characterization of antioxidant activities of several extracts. Eventually, the promising extract was the ethyl acetate (EAc) extract, The analyzed extracts exhibited that antioxidant activities varied considerably as function of extracts. The highest DPPH• scavenging ability was found in Y6-8，which was divided from EAc by silica column, the lowest IC50 value (6.9μg/ml), followed by Y6-9 (11.7μg/ml) and the TEAC of Y6-8 and Y6-9were greater than positive control trolox. The same tendency was observed using ABTS and FRAP assays. These results suggest that the H. rhomboidea shows a better antioxidant activity, especially in the EAc extract and these results would be a guide in the selection of potential cultivars which are used in food and chemical industry and fresh market.

scholarly journals Chemical Composition, In Vitro and In Silico Antioxidant Potential of Melissa officinalis subsp. officinalis Essential Oil

Antioxidants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1081
Author(s):  
Matilda Rădulescu ◽  
Călin Jianu ◽  
Alexandra Teodora Lukinich-Gruia ◽  
Marius Mioc ◽  
Alexandra Mioc ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
In Silico ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Inhibitory Effect ◽  
Melissa Officinalis ◽  
Β Carotene

The investigation aimed to study the in vitro and in silico antioxidant properties of Melissa officinalis subsp. officinalis essential oil (MOEO). The chemical composition of MOEO was determined using GC–MS analysis. Among 36 compounds identified in MOEO, the main were beta-cubebene (27.66%), beta-caryophyllene (27.41%), alpha-cadinene (4.72%), caryophyllene oxide (4.09%), and alpha-cadinol (4.07%), respectively. In vitro antioxidant properties of MOEO have been studied in 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging, and inhibition of β-carotene bleaching assays. The half-maximal inhibitory concentration (IC50) for the radical scavenging abilities of ABTS and DPPH were 1.225 ± 0.011 μg/mL and 14.015 ± 0.027 μg/mL, respectively, demonstrating good antioxidant activity. Moreover, MOEO exhibited a strong inhibitory effect (94.031 ± 0.082%) in the β-carotene bleaching assay by neutralizing hydroperoxides, responsible for the oxidation of highly unsaturated β-carotene. Furthermore, molecular docking showed that the MOEO components could exert an in vitro antioxidant activity through xanthine oxidoreductase inhibition. The most active structures are minor MOEO components (approximately 6%), among which the highest affinity for the target protein belongs to carvacrol.

In Vitro Antioxidant Activity Metaplexis Japonica Makino Extract and Derivatives

2014 ◽  
Vol 955-959 ◽  
pp. 387-389 ◽  
Author(s):  
Bao Qing Wang
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Acid Test ◽  
Acetone Extracts ◽  
Β Carotene ◽  
Ethyl Acetate Extracts

Antioxidant activities of acetone and ethyl acetate extracts from Metaplexis japonica Makino, one of famous medicine plants in the eastnorth region of China, named luomo in Chinese, were examined by a DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical-scavenging assay and a β-carotene-linoleic acid test. In DPPH, the antioxidant activity of the acetone extracts, ethyl acetate extracts and derivative were IC50 were 313.21, 266.92 and 118.78μg/mL, respectively. In the β-carotene-linoleic acid test, IC50 were 285.09, 351.57 and 123.89μg/mL. It was concluded that Metaplexis japonica Makino and its derivatives might be a potential natural source of antioxidants .

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