scholarly journals Molecular Detection and Typing of Cervical Human Papillomavirus in a Selected Population in Bayelsa State: Comparison with the National and International Trend and Clinical Significance

2021 ◽  
pp. 1-16
Author(s):  
Y. I. Oboma ◽  
A. A. Ngokere ◽  
Y. M. Tatfeng ◽  
S. I. Musa ◽  
I. A. Ibrahim ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Single Point ◽  
Cervical Dysplasia ◽  
Life Time ◽  
Hpv Infection ◽  
Single Point Mutation ◽  
Cervical Lesions ◽  
Level Of Knowledge ◽  
High Risk Hpv

Background: The link between cervical lesions and human Papillomavirus (HPV) 16 and 18 is well established, but the magnitude of the risk of association and the importance of other high-risk hpv types is uncertain in Bayelsa state. Aims: The study was aimed at detecting and typing of cervical hpv among selected subjects, establish the relationship between cervical dysplasia and hpv and also asses the level of knowledge of hpv, perception and attitude of women in Bayelsa State. Materials and Methods: Questionnaires were used to assess the level of knowledge of hpv and cervical cancer. Papanicolaou stain for cervical cytology and Haematoxylin and Eosin stain used to study general tissue structure. Nested PCR was used to detect and multiplex PCR for typing. Results: The prevalence of hpv spectrum among participants was 52% while high risk hpv was 24%. Five (5) subtypes were identified. The subtypes identified were hpv 52(40.4%) most predominant, followed by 51(1.9%), 45(1.9%), 31(1.9%) and hpv 30 (9.6%). The age-specific prevalence showed a peak prevalence of 44.2% in the ages of 25-34 years and lowest in the age group (15-24years). Sequence alignment showed a single point mutation for hpv 45 and several points' mutation for hpv 52 at certain points of the sequenced nucleotides with Sequence no: 53CN12 and SeqH2011055303 and accession no: MG195999 and MG196000. HPV 52 was highest compared to previous studies, national and international. The level of knowledge on hpv infection and cancer of the cervix was low among subjects with a percentage score of 38.9%. Among other variables studied, life time sexual partners showed a statistically significant relationship in the prevalence of hpv (OR=0.02, P˂0.01). Perceived seriousness on hpv infection was high among hpv positive subjects compared with hpv negative participants ( =16.39, p ˂0.01). Conclusion: The emergence of hpv 52 in the study area requires public health attention and thus urgent need for local hpv vaccines production.

scholarly journals Molecular characterization of high-risk human papillomavirus (HR-HPV) in women in Lomé, Togo

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Akouélé P. Kuassi-Kpede ◽  
Essolakina Dolou ◽  
Théodora M. Zohoncon ◽  
Ina Marie Angèle Traore ◽  
Gnatoulma Katawa ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
West Africa ◽  
Public Health Problem ◽  
Hpv Infection ◽  
Cervical Lesions ◽  
Hpv Genotypes ◽  
Precancerous Cervical Lesions ◽  
High Risk Hpv ◽  
First Time

Abstract Background The causative agent of cervical cancer referred to as Human papillomavirus (HPV) remains a real public health problem. Many countries in West Africa, such as Togo have no data on the high-risk HPV (HR-HPV) infection and genotypes distribution. In order to fill the knowledge gap in the field in Togo, the main objective of this study was to determine the prevalence of pre-cancerous lesions of the cervix and HR-HPV genotypes among Togolese women. Methods Samples were collected from 240 women by introducing a swab in the cervix. Then, the screening of precancerous cervical lesions using the visual inspection with acetic acid and lugol (VIA / VIL) was conducted. The HR-HPV genotypes were characterised by real-time multiplex PCR. Results Out of 240 women recruited, 128 (53.3%) were infected by HR-HPV. The most common genotypes were HPV 56 (22.7%), followed by HPV 51 (20.3%), HPV 31 (19.5%), HPV 52 (18.8%) and HPV 35 (17.2%). The least common genotypes were HPV 33 (2.3%) and HPV 16 (2.3%). Among the women, 1.3% (3/240) were positive to VIA/VIL. Conclusion This study allowed HR-HPV genotypes to be characterised for the first time in Lomé, Togo. This will help in mapping the HR-HPV genotypes in West Africa.

scholarly journals Detection and estimation of human papillomavirus viral load in patients with cervical lesions

2014 ◽  
Vol 39 (2) ◽  
pp. 86-90 ◽  
Author(s):  
T Rahman ◽  
S Tabassum ◽  
M Jahan ◽  
A Nessa ◽  
Dr Ashrafunnessa
Keyword(s):  
Human Papillomavirus ◽  
Viral Load ◽  
High Risk ◽  
Invasive Cervical Cancer ◽  
Hpv Infection ◽  
Cervical Lesions ◽  
Hpv Dna ◽  
Promising Tool ◽  
High Risk Hpv ◽  
Hpv Viral Load

Human papillomavirus (HPV) high risk genotype infection and HPV viral load influences the development of invasive cervical cancer and cervical intra-epithelial neoplasia (CIN). HPV DNA testing for screening of cervical cancers may play a potential role in its early detection and management. The present study detected HPV DNA and estimated HPV viral load in different types of cervical lesions among Bangladeshi women. Using the Hybrid Capture 2 (HC2) assay, HPV DNA was tested among 68 women between 25-70 years of age. A total of 13 (19.1%) cases were positive for HPV DNA. The highest viral load (501 x 10³ copies/ml) was detected in a patient with invasive carcinoma, while the lowest viral load (105 x 10³ copies/ml) was detected from a case of chronic cervicitis. The mean viral load in CIN I was 119.25 x 10³±12.5 x 10³ copies/ml (range: 110 x 10³ - 137 x 10³) and 208.50 x 10³ ± 0.59 x 10³ copies/ml (range: 139 x 10³-305 x 10³) in CIN II / III. Interestingly, HPV DNA was detected from a patient with normal cytological findings. Our study observed a moderate presence of high-risk HPV genotypes among women with cervical lesions. The HPV viral load varied with the age of the patients and stage of cervical lesions. The HC2 assay is a promising tool for diagnosing high-risk HPV infection especially before cytology tests show any abnormality. DOI: http://dx.doi.org/10.3329/bmrcb.v39i2.19648 Bangladesh Med Res Counc Bull 2013; 39: 86-90

scholarly journals Multiple Human Papilloma Virus Infections and Their Impact on the Development of High-Risk Cervical Lesions

2015 ◽  
Vol 59 (5) ◽  
pp. 391-398 ◽  
Author(s):  
Katrina L. Salazar ◽  
Haijun Steve Zhou ◽  
Jiaqiong Xu ◽  
Leif E. Peterson ◽  
Mary R. Schwartz ◽  
...  
Keyword(s):  
High Risk ◽  
Synergistic Effect ◽  
Hpv Infection ◽  
Multiple Infections ◽  
Virus Infections ◽  
Cervical Lesions ◽  
Papilloma Virus ◽  
Cervical Disease ◽  
Intergenotypic Competition ◽  
High Risk Hpv

Objective: Individuals are often infected with multiple genotypes of human papillomavirus (HPV) simultaneously, but the role these infections play in the development of cervical disease is not well established. This study aimed to determine the association of multiple HPV infections with high-risk cervical lesions (hrCLs). Study Design: HPV genotyping was performed on 798 SurePath specimens collected between December 1, 2009, and April 30, 2011. The cases were classified as hrCL (n = 90) or non-hrCL (n = 708) based on cytology diagnoses. The association between hrCL and HPV infection patterns was analyzed. Results: Multiple HPV infections were frequently encountered (38.2%) in the cohort. Increased frequency of hrCLs was associated with a single high-risk HPV (hrHPV) infection. An additive or synergistic effect was not observed for hrCL in multiple HPV infections. The hrCL rates appeared to decrease in various patterns of multiple HPV infections, but the reduction was not statistically significant. Conclusions: Multiple HPV infections are common with no additive or synergistic effect on the development of hrCL. Conversely, reduced hrCL rates were observed in various patterns of multiple HPV infections compared to their single-genotype infection counterparts, suggestive of possible intergenotypic competition or more effective immune response triggered by multiple infections. Further studies in larger cohorts are needed.

The genomic distribution map of human papillomavirus in China

2021 ◽  
Author(s):  
Ling Chen ◽  
Yan Dong ◽  
Jiao Li ◽  
Jinqiu Zhao ◽  
Li Xu ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Infection Rate ◽  
Subgroup Analysis ◽  
Hpv Infection ◽  
Single Type ◽  
Distribution Map ◽  
Double Infection ◽  
Genomic Distribution ◽  
High Risk Hpv

Abstract In this study, a total of 301,880 woman were recruited from 4 different regions of China. The overall prevalence of HPV was 18.01 %. The high-risk HPV infection rate was 79.14%, the low-risk HPV infection rate was 12.56 %, and the mixed HPV infection rate was 8.30%. The most common 4 HR HPV subtypes were HPV-52, 16, 58 and 53, which accounted for 20.49 %, 19.93 %, 14.54 % and 10.01 %. In LR HPV genotype, HPV-6 ranked the highest (28.17 %), followed by HPV-81 (9.09 %) and HPV-11 (3.78 %). HPV genotype subgroup analysis also showed that single-type infections had the highest prevalence rate (77.26 %) among HPV positive individuals. Among muti-infection genotype, double infection was the most common with frequencies of 76.04 %. This large report showed that the overall prevalence of HPV was high in China, whose distribution exhibits different patterns across different particular age and regions. Viral genotypes HPV 53, 6 were frequently detected in this population, which is worth of significant clinical attention.

Carriage of high-risk human papillomavirus infection in male subjects in Ouagadougou, Burkina Faso

2019 ◽  
Author(s):  
Sya Forazoun Bienvenue Tamini ◽  
Theodora Mahoukèdè Zohoncon ◽  
Serge Theophile Soubeiga ◽  
Florencia Wendkuuni Djigma ◽  
Ina Marie Angele Traore ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Burkina Faso ◽  
Public Health Problem ◽  
Hpv Infection ◽  
Cross Sectional Study ◽  
Multiple Infections ◽  
Cross Sectional ◽  
High Risk Hpv ◽  
Male Subjects

Abstract Background Human papillomavirus (HPV) infection is a public health problem in Africa because of its frequency and its various consequences in both men and women. In Burkina Faso, there is little data on the carriage of HPV infection in male subjects. This study aimed to determine the carriage of HPV infection in male subjects in Ouagadougou, Burkina Faso.Methods This was a cross-sectional study conducted from December 2015 to September 2016. During this study period, a total of 124 male subjects who gave informed consent were recruited as part of a fertility assessment. Semen samples were analyzed by Real- Time PCR using Real TM Quant High Risk Screen HPV kit to detect 14 high-risk HPV genotypes. The Chi square test was used for comparisons.Results Of the 124 male subjects, 22 were positive for at least one high-risk HPV, with a prevalence of 17.7%. HPV 56 was the most represented genotype with a frequency of 20%, followed by HPV 39 (11%) and HPV 68 (11%), HPV 16 (8%). The genotypes HPV 18, HPV33, HPV35, HPV51 and HPV52 also had the same frequency of 6% each and the HPV 31, 45, 59 and 66 had the same frequency of 3% each. Of the 22 positive cases, 10 were multiple infections. Spermocytogram of patients showed at least one abnormality in 87.9% of cases. Necrozoospermia and oligozoospermia were the most abnormalities observed with 42% and 40% respectively followed by asthenospermia (18%); teratozoospermia (16%) and azoospermia (14%).Conclusion This study showed that like women, men are infected by HPV with a relatively high prevalence. In addition, the predominant genotypes in the male subjects of this study are not those targeted by the HPV vaccines available in our countries. Hence the need to reinforce the strategies for controlling HPV infection and HPV prophylactic vaccination for young boys.

scholarly journals High-risk Human Papillomavirus (16, 18) are not the most common genotypes associated with cervical pre-cancer lesions: a retrospective study at a University Hospital in the Eastern-Province of Saudi Arabia

2020 ◽  
Author(s):  
Haitham Kussaibi ◽  
Reem Al Dossary ◽  
Ayesha Badar ◽  
Aroub Omar Muammar ◽  
Raghad Ibrahim Aljohani
Keyword(s):  
Saudi Arabia ◽  
Human Papillomavirus ◽  
Statistical Analysis ◽  
High Risk ◽  
Pap Smear ◽  
University Hospital ◽  
Pap Smears ◽  
Cervical Lesions ◽  
High Risk Hpv ◽  
Intraepithelial Lesions

AbstractObjectiveHigh-risk HPV (human papillomavirus) is found to be responsible for 4.5% of all cancer, especially cervical cancer. The prevalence of high-risk HPV associated with cervical lesions is not well- known in Saudi Arabia. This study aims to highlight the genotypes of high-risk HPV associated with pre- malignant cervical lesions.MethodsOver 6 years (2013 - 2018), 5091 Pap (Papanicolaou) smears results and 170 high-risk HPV test results were collected from the Information System at King Fahd University Hospital. Statistical analysis was performed using the software SPSS (Statistical Package for Social Sciences).ResultsOut of 5091 Pap smears, only 1.89% (n=96) were abnormal; 0.18% (n=9) were malignant (7 Squamous cell carcinomas and 2 adenocarcinomas), while 1.7% (n=87) showed pre-cancerous lesions, 44 ASCUS (Atypical Squamous Cells of Undetermined Significance), 17 LSIL (Low-grade Squamous Intraepithelial Lesions), 12 HSIL (High-grade Squamous Intraepithelial Lesions), and 14 AGC (Atypical Glandular Cells). Out of 170 patients co-tested for high-risk HPV, only 13.5% (n=23/170) had positive results (5 cases were positive for HPV16, 1 case was positive for both HPV16 and 18, while the remaining 17 cases were positive for high-risk HPV other than 16 or 18), among them, 6.47% (n=11/170) had normal Pap smear, while 7.06% (n=12/170) patients had abnormal Pap smear; 4 ASCUS, 6 LSIL and 2 HSIL. Statistical analysis showed a significant correlation between HPV findings and the Pap smear results (P- value 0.000), however, no significant correlation was found with the patients’ age and/or nationality.DiscussionOur study showed a unique distribution of high-risk HPV genotypes which reflects different geographical infection patterns. Furthermore, the high association of high-risk HPV with normal Pap smears highlights the need, for all women at risk, to be co-investigated for high-risk HPV. These findings could help in customizing regional vaccine-combinations and screening programs.

scholarly journals Current studies on human papillomavirus in Saudi Arabia

2015 ◽  
Vol 9 (06) ◽  
pp. 571-576 ◽  
Author(s):  
Fatimah Saeed Alhamlan ◽  
Ahmed A Al-Qahtani ◽  
Mohammed N Al-Ahdal
Keyword(s):  
Cervical Cancer ◽  
Saudi Arabia ◽  
Human Papillomavirus ◽  
High Risk ◽  
Hpv Infection ◽  
World Health ◽  
Cervical Lesions ◽  
Hpv Types ◽  
Health Organization ◽  
Low Prevalence

Human papillomavirus (HPV) infection is a significant etiological factor and an important prognosticator in cervical cancer. Indeed, researchers worldwide have confirmed these roles for high-risk HVPs in over 70% of cervical cancer cases. According to the World Health Organization, approximately 561,200 new cancer cases (5.2% of all new cancers) are attributed to HPV infection. Over 120 types of HPV are classified further as either low-risk HPV (LR-HPV) or high-risk HPV (HR-HPV) based on their oncological potential of transforming cells. The LR-HPV types cause benign hyperproliferative lesions (i.e. genital warts) while the HR-HPV types are strongly associated with premalignant and malignant cervical lesions. Data on the prevalence of HPV, survival of infected patients, and mortality rate are scarce in Saudi Arabia. The unsubstantiated assumption of a low prevalence of HPV in Saudi Arabia has contributed to limiting HPV research in this conservative country. Therefore, the goal of this review is to shed light on the current HPV research being conducted and the prevalence of HPV in Saudi Arabia.

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