In vitro Cytotoxic Activity of Hydro Ethanolic Extract of Delonix regia (Bojer ex. Hook.) Flowers on Cancer Cell Lines

The synthesis of A-azepanobetulinic acid N-methylpiperazinylamide was performed through a series of transformations (oximation, Beckmann rearrangement, reduction) of previously synthesized betulonic acid N-methylpiperazinylamide. In vitro cytotoxic activity was detected for the obtained compound against a number of cancer cell lines, and its potential was revealed as an antibacterial agent.

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Evaluation of the In Vitro Cytotoxic Activity of Caffeic Acid Derivatives and Liposomal Formulation against Pancreatic Cancer Cell Lines

Materials ◽

10.3390/ma13245813 ◽

2020 ◽

Vol 13 (24) ◽

pp. 5813

Author(s):

Magdalena Zaremba-Czogalla ◽

Anna Jaromin ◽

Katarzyna Sidoryk ◽

Agnieszka Zagórska ◽

Marcin Cybulski ◽

...

Keyword(s):

Pancreatic Cancer ◽

Cytotoxic Activity ◽

Caffeic Acid ◽

Cell Lines ◽

Cancer Cell ◽

Pancreatic Cancer Cell ◽

Cancer Cell Lines ◽

Liposomal Formulation ◽

Caffeic Acid Derivatives

Pancreatic cancer belongs to the most aggressive group of cancers, with very poor prognosis. Therefore, there is an important need to find more potent drugs that could deliver an improved therapeutic approach. In the current study we searched for selective and effective caffeic acid derivatives. For this purpose, we analyzed twelve compounds and evaluated their in vitro cytotoxic activity against two human pancreatic cancer cell lines, along with a control, normal fibroblast cell line, by the classic MTT assay. Six out of twelve tested caffeic acid derivatives showed a desirable effect. To improve the therapeutic efficacy of such active compounds, we developed a formulation where caffeic acid derivative (7) was encapsulated into liposomes composed of soybean phosphatidylcholine and DSPE-PEG2000. Subsequently, we analyzed the properties of this formulation in terms of basic physical parameters (such as size, zeta potential, stability at 4 °C and morphology), hemolytic and cytotoxic activity and cellular uptake. Overall, the liposomal formulation was found to be stable, non-hemolytic and had activity against pancreatic cancer cells (IC50 19.44 µM and 24.3 µM, towards AsPC1 and BxPC3 cells, respectively) with less toxicity against normal fibroblasts. This could represent a promising alternative to currently available treatment options.

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Straightforward synthesis of a novel ring-fused pyrazole-lactam and in vitro cytotoxic activity on cancer cell lines

European Journal of Medicinal Chemistry ◽

10.1016/j.ejmech.2016.04.006 ◽

2016 ◽

Vol 117 ◽

pp. 1-7 ◽

Cited By ~ 13

Author(s):

G. Bertuzzi ◽

E. Locatelli ◽

D. Colecchia ◽

P. Calandro ◽

B.F. Bonini ◽

...

Keyword(s):

Cytotoxic Activity ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines

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In vitro antiproliferative/cytotoxic activity on cancer cell lines of a cardanol and a cardol enriched from Thai Apis mellifera propolis

BMC Complementary and Alternative Medicine ◽

10.1186/1472-6882-12-27 ◽

2012 ◽

Vol 12 (1) ◽

Cited By ~ 42

Author(s):

Dungporn Teerasripreecha ◽

Preecha Phuwapraisirisan ◽

Songchan Puthong ◽

Kiyoshi Kimura ◽

Masayuki Okuyama ◽

...

Keyword(s):

Apis Mellifera ◽

Cytotoxic Activity ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines

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Synthesis of pyrophosphate analogues and their cytotoxic activities

Journal of Chemical Research ◽

10.1177/17475198211043439 ◽

2021 ◽

pp. 174751982110434

Author(s):

Van-Tai Nguyen ◽

Minh-Quan Pham ◽

Thi-Ha Vu ◽

Thi-Hong-Ha Tran ◽

Duy-Tien Doan ◽

...

Keyword(s):

Cytotoxic Activity ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Dipolar Cycloaddition ◽

Cytotoxic Activities ◽

Cycloaddition Reactions

Six pyrophosphate analogues are prepared from zerumbone, murrayafoline A, acridone, and 4-hydroxycoumarin via 1,3-dipolar cycloaddition reactions. Their in vitro cytotoxic activity is evaluated against HepG2, LU-1, and HeLa cancer cell lines. Among them, diisopropyl ((ethoxy((4-((1-methoxy-3-methyl-9 H-carbazol-9-yl)methyl)-1 H-1,2,3-triazol-1-yl)methyl)phosphoryl)methyl)phosphonate (6a) and diisopropyl ((ethoxy((4-(((3-methyl-9 H-carbazol-1-yl)oxy)methyl)-1 H-1,2,3-triazol-1-yl)methyl)phosphoryl)methyl)phosphonate (6b) are found to show activity against HepG2, LU-1, and HeLa cancer cell lines, with IC50 values ranging from 7.31 to 17.88 μM.

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Cytotoxic Activities in Vitro of Flower Extracts of Three Species of Aloe Growing in Yemen: Aloe Rubroviolaceae, Aloe Vera and Aloe Sabaea, against Eleven Types of Cancer Cell Lines

Journal of Clinical Research and Reports ◽

10.31579/2690-1919/187 ◽

2021 ◽

Vol 8 (5) ◽

pp. 01-10

Author(s):

Hassan A. Al-Shamahy

Keyword(s):

Cytotoxic Activity ◽

Cancer Cells ◽

Cell Lines ◽

Cancer Cell ◽

Human Cancer ◽

Aloe Vera ◽

Cancer Cell Lines ◽

Myelogenous Leukemia ◽

Methanolic Extracts

Background and aims: Natural products, especially plant extracts, have opened up great opportunities in the field of drug progress due to their chemical variety. The genus Aloe has long been used for medicinal uses in countless parts of the world. This study was designed to investigate the phytochemicals and anti-cancer capabilities of Aloe rubroviolaceae, Aloe vera and Aloe sabaea flowers. Materials and Methods: The methanolic extracts of three types of plants traditionally used in Yemen to treat a variety of diseases have been tested in vitro for their potential anticancer activity on different human cancer cell lines. The cytotoxic activity of the methanolic extracts of tested plants was determined using eleven strains of human cancer cells, namely: MCF-7 (breast cancer), PC-3 (prostate cancer), HEP-2 (human epithelial carcinoma), MNFS-60 (myelogenous leukemia), CACO (intestinal cancer), A-549 (lung adenocarcinoma), HeLa (cervical cancer), RD (rhabdomyosarcoma),HepG2 (hepatocellular carcinoma), HCT-116 (colon cancer), and CHO-K1 (Chinese hamster ovary). A colorimetric sulforhodamine B assay was used to evaluate the in vitro cytotoxic activity of different extracts. Growth inhibition of 50% (IC50) for each extract was calculated from the optical density of treated and untreated cells. Doxorubicin, a broad-spectrum anticancer drug was used as a positive control. Results: More interesting cytotoxic activity was observed for Aloe vera extract more than Aloe sabaea and Aloe rubroviolaceae, extract. Conclusions: This study provides a preliminary screening for anti-proliferative activity of various Aloe species flowers extracts on different cancer cell lines. Different extracts of Aloe species significantly inhibit the growth of various cancer cell lines in a concentration-dependent manner. Further investigations are required to understand the possible mechanism(s) of action of these extract on various cancer cells and isolation of active phyto-chemicals.

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