Effect of Ethanolic Leaf Extract of Psidium guajava Linn. (Guava) in Alloxan-Induced Diabetic Rats

Objective: This study was conducted to scientifically evaluate the antidiabetic and antioxidant effects of ethanolic leaf extract of Erythrina abbysinica (EEA).Methods: Acute and sub-chronic effects of EEA at 100, 200, and 400 mg/kg/bwt and glibenclamide (GL) at 5 mg/kg/bwt. were evaluated in both normal and streptozotocin (STZ)-induced diabetic male Wistar rats (250–300 g). The acute studies were performed using oral glucose tolerance test (OGTT). In sub-chronic studies, animals were orally administered with EEA and GL daily for 6 w. Brine shrimp assay was used to determine the toxicity of EEA. 1, 1-diphenyl-2-picrylhydrazyl, ferric reducing capacity of plasma, and thiobarbituric acid reactive substances assays were used to determine antioxidant properties of EEA.Results: Following OGTT, EEA significantly (p<0.05) and dose-dependently (100, 200, and 400 mg/kg/bwt) decreased blood glucose levels in both normal and STZ-induced diabetic rats when compared with positive and negative control counterparts at all-time points, whereas GL significantly (p<0.05) decreased blood glucose only in normal rats but not in diabetic rats. Daily, oral administration of EEA for 6 w significantly (p<0.05) and dose-dependently (100, 200, and 400 mg/kg/bwt) decreased blood glucose levels in STZ-induced diabetic rats when compared with the diabetic control group. EEA revealed weak toxicity with a lethal concentration50 value of 997 μg/ml). Furthermore, EEA showed significant free radical scavenging, total antioxidant, and anti-lipid peroxidative capacities.Conclusion: The study has shed more light on the scientific basis for the use of E. abbysinica in management of diabetes in some communities of Eastern Cape of South Africa.

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IN VITRO EVALUATION OF ANTI INFLAMMATORY ACTIVITY OF METHANOLIC AND ETHANOLIC LEAF EXTRACT OF PSIDIUM GUAJAVA

World Journal of Current Medical and Pharmaceutical Research ◽

10.37022/wjcmpr.2020.2214 ◽

2020 ◽

Vol 02 (02) ◽

pp. 159-165

Author(s):

SANTANU KUMAR HOTTA ◽

NEELIMA N

Keyword(s):

Leaf Extract ◽

Psidium Guajava ◽

Inflammatory Activity ◽

In Vitro Evaluation ◽

Anti Inflammatory ◽

Ethanolic Leaf Extract

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Evaluation of potentials of aqueous ethanolic extract of Psidium guajava on reproductive functions of male Albino rats

Sokoto Journal of Veterinary Sciences ◽

10.4314/sokjvs.v19i3.2 ◽

2021 ◽

Vol 19 (3) ◽

pp. 174-181

Author(s):

S.A. Abwage ◽

S.T. Agu ◽

S.A. Saganuwan ◽

A.H. Abu

Keyword(s):

Body Weight ◽

Leaf Extract ◽

Ethanolic Extract ◽

Psidium Guajava ◽

Reproductive Organs ◽

Sperm Parameters ◽

Albino Rats ◽

Reproductive Parameters ◽

Aqueous Ethanolic Extract ◽

Ethanolic Leaf Extract

The therapeutic utilization of plants for managing several diseases by people of all continents, especially Africa, is as old as tradition. The objective of this study was to evaluate the potentials of aqueous ethanolic leaf extract of Psidium guajava on male reproductive parameters. Twenty-four male Albino rats were randomly assigned into four groups of six rats per group. Rats in the control group were administered Tween 20®. Rats in groups 2, 3, and 4 were administered Psidium guajava aqueous ethanolic leaf extract orally at the doses of 100, 200, and 400 mg/kg body weight, respectively, once daily for 60 days. The body weights of the rats were determined at the beginning and end of the experiment. Sperm parameters and some reproductive organs weight of each rat was also determined, some organs were collected for histopathology. Assay for follicle-stimulating hormone, luteinizing hormone, and testosterone was done using Enzyme-Linked Immunosorbent methods. Some reproductive organs were collected for histopathological analyses. The results showed a dose-dependent increase (p<0.05) in the weight of male reproductive organs, sperm parameters, and hormones. The extract significantly increased (P < 0.05) serum testosterone in the group treated 400 mg/kg body weight when treated groups were compared with control. Furthermore, the results of FSH and LH revealed a significant (p < 0.05) increase when the treated groups were compared with the control. The histopathological analysis did not reveal any form of damage to the architectural integrity of the testis. However, there was degenerating germinal epithelium in the group administered 400 mg/kg of the aqueous ethanolic leaf extract of P. guajava. Conclusion: aqueous ethanolic extract of P. guajava has positive effects on male reproductive parameters.

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ANNONA MURICATA LINN. ETHANOLIC LEAF EXTRACT AMELIORATES REPRODUCTIVE COMPLICATIONS IN STREPTOZOTOCIN-INDUCED DIABETIC WISTAR RATS

Journal of Natural Sciences Engineering and Technology ◽

10.51406/jnset.v18i1.2040 ◽

2020 ◽

Vol 18 (1) ◽

pp. 166-175

Author(s):

O. E. ADELEYE ◽

N. A. ABOAJAH ◽

A. I. ADELEYE ◽

E. A.O. SOGEBI ◽

F. M. MSHELBWALA ◽

...

Keyword(s):

Blood Glucose ◽

Wistar Rats ◽

Leaf Extract ◽

Sperm Count ◽

Fasting Blood Glucose ◽

Diabetic Rats ◽

Male Rats ◽

Annona Muricata ◽

Major Health Problem ◽

Ethanolic Leaf Extract

Diabetes mellitus is an endocrine and metabolic disorder of humans and animals characterized by hyperglycemia and low blood insulin levels or insensitivity of target organs to insulin and it’s a major health problem affecting patient’s quality of life due to its many complications. Infertility is one of the major secondary complications in diabetes. Although numerous drugs have been used for intervention studies on diabetes-induced infertility worldwide, there are currently no treatments for diabetes associated infertility in humans. This study was performed to investigate the effects of Annona muricata ethanolic leaf extract (AMELE) on fertility of male diabetic rats and levels of blood glucose. Twenty male Wistar rats (150-200g) were randomly distributed into 4 groups (n=5) treated thus: CTRL (control), DNT, DT1 and DT2 (diabetic, single intraperitoneal injection, streptozotocin, 60 mg/kg). Group DT1 and DT2 received AMELE orally at 100 mg/kg and 200 mg/kg respectively daily for fourteen days. Data were analysed using ANOVA at α0.05. The animals were sacrificed after 2 weeks via thiopental injection and testicular weights were recorded. Fasting blood glucose was determined using a digital glucometer. Sperm count, motility, viability and morphology were assessed microscopically. Testes were histologically evaluated. The results showed that oral administration of AMELE at 100 mg/kg and 200 mg/kg to diabetic male rats for fourteen days significantly decreased blood glucose level and also ameliorated diabetes-induced decreases in sperm functions in streptozotocin-induced diabetic male rats.

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