The Effect of Ginger on the Invasion and Migration of Glioma Cells

Background: This work studies the effect of different concentrations of soaked ginger on the ability of the U87 glioma cells to invade collagen in a three dimension (3 D) invasion model and compare it with its effect on the ability of the same cell line to migrate in two-dimension (2 D) scratch assay. Methods: The hanging drop spheroids in 3D invasion assay were used to investigate the in invasion of the U87 cells. The 2D scratch assay was used to investigate the migration of the same cell line. Results: Gradual effect of the soaked ginger was noticed on the inhibition of the invasion of U87 in collagen and on the inhibition of the migration of the same cell line in scratch assay. Conclusion: The results in this article are promising and encourage further studies to investigate the effect of ginger active ingredients on tumour progression.

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CTR9-Mediated JAK2/STAT3 Pathway Promotes The Proliferation, Migration and Invasion of Human Glioma Cells

10.21203/rs.3.rs-524456/v1 ◽

2021 ◽

Author(s):

Yang Xu ◽

Jiaguo Chen ◽

Gao He ◽

Yuhai Zhang

Keyword(s):

Protein Modification ◽

Cell Function ◽

Glioma Cells ◽

Migration And Invasion ◽

Stat3 Pathway ◽

Invasion And Migration ◽

The Public ◽

Significant Difference ◽

And Migration ◽

U87 Cells

Abstract Background CTR9 (Cln three requiring 9) has been reported to be implicated in protein modification and oncogenesis of several human cancers. However, the protein expression and mechanism of CTR9 in glioma progression remain unclear. Methods We analyzed mRNA expression of CTR9 and CTR9-related survival curves in the public database. Then we detected CTR9 expression in glioma tissues and constructed U251 and U87 cells with stable silencing or overexpression of CTR9. Cell function tests and Western blot were conducted to explore the effects of CTR9 on glioma proliferation, invasion and migration, as well as the specific mechanism. All the date was presented as means ± SEM. Two-sample t-test and one-way analysis of variance (ANOVA) were used to identify whether there was a significant difference between each group of data. Results We found that CTR9 was overexpressed in glioma and inversely associated with glioma patient survival. The results manifested that knockdown of CTR9 suppressed the proliferation, migration and invasion of glioma cells, while overexpression facilitated them. The underlying molecular mechanism may involve the regulation of JAK2/STAT3 pathway by CTR9. Conclusion Our present study indicates that CTR9 is highly expressed in glioma and related to glioma grading and prognosis. CTR9 regulates malignant behaviors of glioma cells by activating JAK2/STAT3 pathway. Therefore, CTR9 is a promising biomarker for the diagnosis,targeted therapy and prognosis evaluation of glioma.

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Long noncoding RNA OIP5-AS1 targets Wnt-7b to affect glioma progression via modulation of miR-410

Bioscience Reports ◽

10.1042/bsr20180395 ◽

2019 ◽

Vol 39 (1) ◽

Cited By ~ 11

Author(s):

Wei-Li Sun ◽

Tian Kang ◽

Yuan-Yu Wang ◽

Jian-Ping Sun ◽

Chen Li ◽

...

Keyword(s):

Long Noncoding Rna ◽

Noncoding Rna ◽

Biological Effects ◽

Glioma Cells ◽

Phase Cell ◽

Luciferase Reporter ◽

Invasion And Migration ◽

Reporter Assays ◽

And Migration ◽

U87 Cells

Abstract The present study was undertaken to investigate the underlying mechanisms of long noncoding RNA OIP5-AS1 via regulating miR-410 to modulate Wnt-7b in the progression of glioma. To address this problem, we measured the expression of OIP5-AS1 and miR-410 in glioma tissues by qRT-PCR. Glioma U87 cells were transfected with OIP5-AS1 siRNA or miR-410 inhibitors. The targeting relationships among miR-410, OIP5-AS1 and Wnt-7b were verified by luciferase reporter assays. Western blotting was employed to determine the expression of Wnt-7b/β-catenin pathway-related proteins, while MTT, flow cytometry, Transwell assays and wound-healing assays were used to measure the biological characteristics of glioma cells. The results showed that OIP5-AS1 expression was higher and miR-410 was lower in glioma tissues. Luciferase reporter assays confirmed a targeting relationship between OIP5-AS1 and miR-410, as well as between miR-410 and Wnt-7b. Silencing OIP5-AS1 reduced cell proliferation, invasion and migration of glioma U87 cells and led to depressed expression levels of miR-410, Wnt-7b, p-β-catenin, GSK-3β-pS9, c-Myc and cyclin D1. Furthermore, down-regulation of OIP5-AS1 induced G0/G1 phase cell cycle arrest and apoptosis of glioma cells. Inhibitors of miR-410 abolished the biological effects of OIP5-AS1 siRNA in glioma cells. In vivo, OIP5-AS1 knockdown also inhibited tumor growth. Taken together, this research suggested that silencing OIP5-AS1 may specifically block the Wnt-7b/β-catenin pathway via targeted up-regulating miR-410, thereby inhibiting growth, invasion and migration while promoting apoptosis in glioma cells.

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Effects of miR-99a on the migration and proliferation of glioma cells

Revista Romana de Medicina de Laborator ◽

10.2478/rrlm-2018-0005 ◽

2018 ◽

Vol 26 (2) ◽

pp. 145-152

Author(s):

Yifan Xu ◽

Tianyu Lu ◽

Wu Xu ◽

Yuxiang Dai ◽

Weibang Liang ◽

...

Keyword(s):

Cell Line ◽

Mtt Assay ◽

Glioma Cell ◽

Glioma Cells ◽

Glioma Cell Line ◽

Scratch Assay ◽

Knock Down ◽

And Migration ◽

Proliferation And Migration

Abstract Background To evaluate the effects of miR-99a on the migration and proliferation of glioma cells. Materials and Methods: Glioma cell line LN229 with stable up-regulation of miR-99a was constructed by transfection of hsa-miR-99a mimics, and cells with stable miR-99a knock-down were established by transfection of hsa-miR-99a inhibitor. The proliferation capacities of two groups were detected by the MTT assay, and their migration capacities were detected by the scratch assay. Results: LN229 cells with stable up-regulation and knock-down of miR-99a were successfully constructed. Up-regulating miR-99a inhibited the proliferation and migration of glioma cells, but knocking down this gene promoted their proliferation and migration. Conclusion: MiR-99a significantly affected the proliferation and migration of glioma cells, as a potentially eligible target for glioma therapy.

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Discovery of mitochondria-targeting berberine derivatives as the inhibitors of proliferation, invasion and migration against rat C6 and human U87 glioma cells

MedChemComm ◽

10.1039/c4md00264d ◽

2015 ◽

Vol 6 (1) ◽

pp. 164-173 ◽

Cited By ~ 15

Author(s):

Shengnan Fu ◽

Yanqi Xie ◽

Jue Tuo ◽

Yalong Wang ◽

Wenbo Zhu ◽

...

Keyword(s):

Glioma Cells ◽

Invasion And Migration ◽

And Migration ◽

Berberine Derivatives ◽

Mitochondria Targeting ◽

U87 Cells

This research aims to synthesize lipophilic berberine derivatives and evaluate their antiglioma effects on C6 and U87 cells.

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The Effect of Curcumin on the Invasion and Migration of Glioma Cells

European Journal of Medicinal Plants ◽

10.9734/ejmp/2020/v31i730249 ◽

2020 ◽

pp. 11-16

Author(s):

Manar Zraikat ◽

Munir Gharaibeh ◽

Tasneem Alshelleh

Keyword(s):

Cell Line ◽

Curcuma Longa ◽

Inhibitory Effect ◽

Glioma Cell Line ◽

Scratch Assay ◽

Invasion And Migration ◽

School Of Medicine ◽

3D Collagen ◽

And Migration ◽

The University

Aim: This study investigated the effect of curcumin, from Curcuma longa, on the invasion of U87 glioma cell line spheres in 3D collagen model. Furthermore, this study investigated the anti-migration effects of curcumin on the migration of the same cell line in scratch assay. Place and Duration of Study: Department of Pharmacology, School of Medicine, The University of Jordan, Amman, Jordan, between March 2019 and May 2019. Methods: 3D invasion assay and 2D migration assays were used to fulfill these aims in addition to the Image J program that was used to analyze the area of invasion and the area of migration over the days of applying the assays. Results: The results showed an inhibitory effect of curcumin in all samples tested on both the invasion and migration of U87 cell line. Conclusion: This work adds more proofs on the importance of curcumin as anti-invasive and anti-migration agent and opens the door for more investigative studies.

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GGNBP2 Suppresses the Proliferation, Invasion, and Migration of Human Glioma Cells

Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics ◽

10.3727/096504016x14816726393937 ◽

2017 ◽

Vol 25 (5) ◽

pp. 831-842 ◽

Cited By ~ 4

Author(s):

Ao Zhan ◽

Bo Lei ◽

Honggang Wu ◽

YueTao Wen ◽

Liandong Zheng ◽

...

Keyword(s):

Glioma Cells ◽

Human Glioma ◽

Human Glioma Cells ◽

Invasion And Migration ◽

And Migration

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Up-regulation of miRNA-148a inhibits proliferation, invasion, and migration while promoting apoptosis of cervical cancer cells by down-regulating RRS1

Bioscience Reports ◽

10.1042/bsr20181815 ◽

2019 ◽

Vol 39 (5) ◽

Cited By ~ 3

Author(s):

Ying Zhang ◽

Bingmei Sun ◽

Lianbin Zhao ◽

Zhengling Liu ◽

Zonglan Xu ◽

...

Keyword(s):

Cervical Cancer ◽

Cancer Cells ◽

Cell Line ◽

Hela Cells ◽

Luciferase Reporter ◽

Migration And Invasion ◽

Invasion And Migration ◽

Cervical Cancer Cells ◽

And Migration

Abstract The purpose of the present study is to figure out the role of miRNA-148a (miR-148a) in growth, apoptosis, invasion, and migration of cervical cancer cells by binding to regulator of ribosome synthesis 1 (RRS1). Cervical cancer and adjacent normal tissues, as well as cervical cancer cell line Caski, HeLa, C-33A, and normal cervical epithelial cell line H8 were obtained to detect the expression of miR-148a and RRS1. Relationship between miR-148a and RRS1 expression with clinicopathological characteristics was assessed. The selected Caski and HeLa cells were then transfected with miR-148a mimics, miR-148a inhibitors or RRS1 siRNA to investigate the role of miR-148a and RRS1 on proliferation, apoptosis, colony formation, invasion, and migration abilities of cervical cancer cells. Bioinformatics information and dual luciferase reporter gene assay was for used to detect the targetting relationship between miR-148a and RRS1. Down-regulated miR-148a and up-regulated RRS1 were found in cervical cancer tissues and cells. Down-regulated miR-148a and up-regulated RRS1 are closely related with prognostic factors of cervical cancer. RRS1 was determined as a target gene of miR-148a and miR-148a inhibited RRS1 expression in cervical cancer cells. Up-regulation of miR-148a inhibited cell proliferation, migration, and invasion while promoting apoptosis in Caski and HeLa cells. Our study suggests that miR-148a down-regulates RRS1 expression, thereby inhibiting the proliferation, migration, and invasion while promoting cell apoptosis of cervical cancer cells.

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miR-126 affects the invasion and migration of glioma cells through GATA4

Artificial Cells Nanomedicine and Biotechnology ◽

10.1080/21691401.2016.1226179 ◽

2016 ◽

Vol 45 (6) ◽

pp. 1247-1253 ◽

Cited By ~ 1

Author(s):

Yifan Xu ◽

Wu Xu ◽

Tianyu Lu ◽

Yuxiang Dai ◽

Weibang Liang

Keyword(s):

Glioma Cells ◽

Invasion And Migration ◽

And Migration

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Proteomics Based Identification of Autotaxin As An Anti-Hepatitis B Virus Factor and a Promoter of Hepatoma Cell Invasion and Migration

Cellular Physiology and Biochemistry ◽

10.1159/000487166 ◽

2018 ◽

Vol 45 (2) ◽

pp. 744-760 ◽

Cited By ~ 2

Author(s):

Sha She ◽

Min Yang ◽

Huaidong Hu ◽

Peng Hu ◽

Yixuan Yang ◽

...

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Cell Line ◽

Secretory Proteins ◽

Hepg2 Cell Line ◽

Migration Ability ◽

Invasion And Migration ◽

Hbv Replication ◽

B Virus ◽

And Migration

Background/Aims: Hepatitis B virus (HBV) infection is a major cause of cirrhosis and hepatocellular carcinoma. Therefore, we aimed to obtain further information on HBV pathogenesis, and to search for novel putative molecules for anti-HBV therapy. Methods: We utilized Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) to identify the secretory proteins that are differentially expressed in the HBV DNA-transfected HepG2.2.15 cell line and its parental HepG2 cell line. Immunohistochemistry (IHC) was employed to assess the clinical relevance of the observations. Small interfering (si)RNA-based silencing transfection methods were carried out to study the function of ENPP2. Results: Totally, 133 unique proteins were identified as differentially expressed in HepG2.2.15 cell line compared with HepG2 cell line. Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 precursor (ENPP2) is one of the most significantly up-regulated secretory proteins associated with HBV replication. This differential expression of ENPP2 was further validated by real-time quantitative RT-PCR, Western Blot and immunohistochemical analysis. To study the function of ENPP2, we knockdown ENPP2 expression in HepG2.2.15 cell line by RNA interference. ENPP2 silencing increased HBV replication approximately 2.3-fold by enhancing, via the type I IFN signaling pathway, HBV cccDNA (covalently closed circular DNA) translation into viral RNA. Moreover, attenuation of ENPP2 expression inhibited both the invasion and migration ability of hepatoma cells in vitro via interacting with the molecules in the tumor microenvironment. Conclusion: Our study demonstrates that ENPP2 may be a novel anti-HBV target and indicate that suppression of its expression may inhibit the invasion and migration ability of hepatoma cells.

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