scholarly journals In vitro Anticoagulant Effect of Grape Seed Extract (Vitis vinifera) on Human Normal Blood Sample

2020 ◽  
pp. 40-45
Author(s):  
Somia Attaelseed Hassan ◽  
Zeinab Sayed Abdelaziz ◽  
Mohammed Mobarak Elbasheir ◽  
Wala Eldin Osma Elradi ◽  
Elharam Ibrahim Abd Allah ◽  
...  
Keyword(s):  
Prothrombin Time ◽  
Partial Thromboplastin Time ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Activated Partial Thromboplastin Time ◽  
Anticoagulant Effect ◽  
Normal Blood ◽  
Grape Seeds ◽  
Blood Samples

Background: Scientists have used herbs nowadays for curing many diseases because they are safer and to overcome the side effect of the chemical drugs. Grape is one of the ancient herbs that used for diseases of the heart and blood vessels, high blood pressure, high cholesterol, skin care and many other conditions. Grape seeds have significantly higher concentrations of polyphenols which has anticoagulant and antithrombotic effect. Materials and Methods: In this study 20 normal blood samples from healthy individuals with age range (19-38) years were enrolled in this study]. Prothrombin time (PT) and activated partial thromboplastin time (APTT) tests were performed before adding grape seed extraction (GSE) (as control) and after adding GSE with different concentrations (25%, 50% and 75%). Results: The results revealed that grape seed extract has an anticoagulant effect as proven by the increase of Prothrombin time and Activated partial thromboplastin time results of the blood samples in different concentrations of the extract. The GSE showed a high statistical significant (P= 0.000) in all concentrations of both PT and APTT tests. Conclusion: This study suggests that GSE has a strong anticoagulant effect; so it can potentially be used as a supplementary anticoagulant agent to prevent thrombosis and cardiovascular diseases.

Grape Seeds Extract as Brain Food: A Review

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Souad El Gengaihi ◽  
Doha H. Abou Baker
Keyword(s):  
Antioxidant Activities ◽  
Complex Mixture ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Amyloid Peptide ◽  
Grape Seeds ◽  
Potential Benefits ◽  
Reduced Risk

Interest in the biological role of bioactive compounds present in medicinal herbs has increased over the last years. Of particular interest are plants that have an anti-Alzheimer activities. Several plants can be useful for Alzheimer (AD) management. Such as these which have anti-inflammatory activity, acetylcholinesterase (AChE) inhibitory action, antiapoptotic, slow the aggregation of amyloid peptide and antioxidant activities. Grape seed extract (GSE) is a complex mixture of several compounds, mostly represented by polyphenols and flavonoids. Their consumption is safe and is recognized to exert several health benefits. GS flavonoids have been associated with the reduced risk of chronic diseases, we present some findings on the potential benefits of GSE for the treatment of AD.

Effects of a Pneumatic Tube System on Routine and Novel Hematology and Coagulation Parameters in Healthy Volunteers

2007 ◽  
Vol 131 (2) ◽  
pp. 293-296
Author(s):  
Alexander Kratz ◽  
Raneem O. Salem ◽  
Elizabeth M. Van Cott
Keyword(s):  
Healthy Volunteers ◽  
Prothrombin Time ◽  
Partial Thromboplastin Time ◽  
Activated Partial Thromboplastin Time ◽  
Tube System ◽  
Blood Samples ◽  
Pneumatic Tube ◽  
The Mean ◽  
Clinically Significant ◽  
Pneumatic Tube System

Abstract Context.—Technologic advances affecting analyzers used in clinical laboratories have changed the methods used to obtain many laboratory measurements, and many novel parameters are now available. The effects of specimen transport through a pneumatic tube system on laboratory results obtained with such modern instruments are unclear. Objective.—To determine the effects of sample transport through a pneumatic tube system on routine and novel hematology and coagulation parameters obtained on state-of-the-art analyzers. Design.—Paired blood samples from 33 healthy volunteers were either hand delivered to the clinical laboratory or transported through a pneumatic tube system. Results.—No statistically significant differences were observed for routine complete blood cell count and white cell differential parameters or markers of platelet activation, such as the mean platelet component, or of red cell fragmentation. When 2 donors who reported aspirin intake were excluded from the analysis, there was a statistically, but not clinically, significant impact of transport through the pneumatic tube system on the mean platelet component. There were no statistically significant differences for prothrombin time, activated partial thromboplastin time, waveform slopes for prothrombin time or activated partial thromboplastin time, fibrinogen, or fibrin monomers. Conclusions.—Although further study regarding the mean platelet component may be required, transport through a pneumatic tube system has no clinically significant effect on hematology and coagulation results obtained with certain modern instruments in blood samples from healthy volunteers.

scholarly journals A study on antibacterial effect of grape seed extracts in common clinical and drug resistant isolates

2016 ◽  
Vol 3 (3) ◽  
pp. 165
Author(s):  
Mohanakrishnan Kandasamy ◽  
Sowmya Nasimuddin ◽  
Jeevan Malayan ◽  
Nithyalakshmi J ◽  
Sumathi Gnanadesikan ◽  
...  
Keyword(s):  
Antibacterial Effect ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Clinical Isolates ◽  
Drug Resistant ◽  
Grape Seeds ◽  
Mrsa Strains ◽  
Agar Well Diffusion Assay ◽  
Grape Seed Extracts

<p class="abstract"><strong>Background:</strong> Grape seeds are proposed to have antimicrobial activity, antioxidant effect and various other benefits to mankind. A study was done to assess the antibacterial effect of grape seed extract against common clinical isolates and drug resistant pathogenic strains.</p><p class="abstract"><strong>Methods:</strong> Grape seed extract prepared was investigated for its antibacterial effect against 65 bacterial isolates obtained from clinical specimens by agar well diffusion assay and the results were compared with routinely used antibiotics namely, Gentamicin for the common clinical isolates, Vancomycin for MRSA strains and Amikacin for ESBL organisms respectively.</p><p class="abstract"><strong>Results:</strong> Grape seed extract produced moderate zone of inhibition ranging between 11-15 mm among the 35 test common clinical isolates namely <em>Staphylococcus aureus, Escherichia coli, Klebsiella </em>sp<em> </em>and<em> Pseudomonas aeruginosa</em>.  <em>E.coli </em>showed the highest susceptibility with zone ranging from 12-14 mm with increasing concentration of the extract starting from2 mg/ml to the highest being 20 mg/ml. Among the 30 drug resistant pathogenic strains like MRSA and ESBL producing organisms, the grape seed extract was found to be effective against 3 out of the 10 of MRSA and 2 out of the 10 of ESBL-<em>E.coli</em> at the highest concentration of 20 mg/ml. However, ESBL producing <em>Klebsiella </em>species were found to be resistant even to the highest concentration of the extract.  </p><p><strong>Conclusions:</strong> The results provide evidence that the grape seed extract could be a potential antibacterial agent and this effect can further be made evident with improved methodologies.</p>

Accuracy of drawing coagulation samples from heparinized arterial lines

1993 ◽  
Vol 2 (1) ◽  
pp. 88-95 ◽  
Author(s):  
K Templin ◽  
M Shively ◽  
J Riley
Keyword(s):  
Prothrombin Time ◽  
Partial Thromboplastin Time ◽  
Coronary Angioplasty ◽  
Percutaneous Transluminal Coronary Angioplasty ◽  
Activated Partial Thromboplastin Time ◽  
Arterial Line ◽  
Blood Samples ◽  
Transluminal Coronary Angioplasty ◽  
Percutaneous Transluminal ◽  
Line Sample

OBJECTIVE: To determine the accuracy of activated partial thromboplastin time and prothrombin time studies when samples are drawn through heparinized arterial lines. METHODS: A total sample of 90 grouped blood samples (from 30 subjects) was used. Patients were all male, with a mean age of 65 and were studied within 24 hours of percutaneous transluminal coronary angioplasty. Each patient had three venous control and arterial line sample sets (a total of 90 blood samples) drawn when routinely ordered for monitoring therapy. For the arterial line sample, a discard volume of the deadspace, deadspace + 2 mL, or deadspace + 4 mL was randomly assigned for each sample. The venous control volumes were the same for all three sample sets. RESULTS: A 2 x 3 repeated measures analysis of variance was used to analyze the results. The independent variables were the source of the sample (venous vs arterial) and the discard volume of arterial blood (deadspace, deadspace + 2 mL, deadspace + 4 mL). The dependent variables were the activated partial thromboplastin time and prothrombin time values. Mean arterial activated partial thromboplastin time values were significantly higher than the corresponding venous values. Mean activated partial thromboplastin time values were not significantly different among the discard volumes of blood drawn. However, there was a significant source by volume interaction. Tukey post-hoc comparisons of venous-arterial activated partial thromboplastin time differences among the three volumes showed significant differences between deadspace volume and deadspace + 2 mL, and deadspace volume and deadspace + 4 mL. There was no significant difference between deadspace + 2 mL and deadspace + 4 mL volumes. CONCLUSION: Results indicated that the minimal amount of discard volume for accurate activated partial thromboplastin time values in this population of percutaneous transluminal coronary angioplasty patients was the catheter deadspace volume plus 2 mL (total 3.6 mL).

scholarly journals Effects of Polyphenols from Grape Seeds on Renal Lithiasis

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Felix Grases ◽  
Rafel M. Prieto ◽  
Rafel A. Fernandez-Cabot ◽  
Antonia Costa-Bauzá ◽  
Fernando Tur ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Calcium Oxalate ◽  
Complex Disease ◽  
Urine Analysis ◽  
Calcium Oxalate Monohydrate ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Oxidative Capacity ◽  
Seed Extract ◽  
Grape Seeds

Nephrolithiasis is a complex disease that results from a combination of factors related to both urine composition and kidney morphoanatomy. Development of calcium oxalate monohydrate papillary calculi is linked to initial subepithelial calcification of renal papilla. Progressive tissue calcification depends on preexisting injury and involves reactive oxygen species. Many plant extracts that protect against oxidative stress manifest antilithiasic activity. Our study focused on determining the effects of polyphenols on a lithiasis rat model. Rats were pretreated with polyphenols and grape seed extracts, followed by posterior induction of hyperoxalosis via treatment with ethylene glycol plus NH4Cl. The concentrations of calcium and other elements in kidney were determined, along with histological examination of kidney and 24 h urine analysis. Significant differences were observed in the renal calcium content between the control plus ethylene glycol-treated group and the epicatechin plus ethylene glycol-treated, red grape seed extract plus ethylene glycol-treated, and white grape seed extract plus ethylene glycol-treated groups, with reductions of about 50%. The antioxidant activity of polyphenols extracted from red and white grape seeds may be critical in the prevention of calcium oxalate monohydrate papillary calculus formation, particularly if calculi are induced by lesions caused by cytotoxic compounds with oxidative capacity.

scholarly journals Dual Anticoagulant/Antiplatelet Activity of Polyphenolic Grape Seeds Extract

Nutrients ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 93 ◽  
Author(s):  
Michal Bijak ◽  
Agnieszka Sut ◽  
Anna Kosiorek ◽  
Joanna Saluk-Bijak ◽  
Jacek Golanski
Keyword(s):  
Blood Platelets ◽  
Adenosine Diphosphate ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Potential Effect ◽  
In Vitro Models ◽  
Seed Extract ◽  
Grape Seeds ◽  
Induced Aggregation

Because of the side-effects of commonly used anti-platelet and anticoagulant drugs, investigations into plant substances with similar activities are very common. Based on our own studies in recent years, we estimate that it is possible to use natural compounds to both inhibit coagulation pathway enzymes and to reduce blood platelets’ activation. As such, in our current study we wanted to verify the anti-platelet and anticoagulant properties of grape seed extract (GSE) using in vitro models. During our analysis, the following parameters were analyzed: Coagulation times, thromboelastometry assays (coagulation time, clot formation time and maximum clot firmness), aggregation of platelets and phosphorylation of vasodilator-stimulated phosphoprotein (VASP). Adenosine diphosphate (ADP)-induced aggregation was lower in GSE 7.5 µg/mL as well as in GSE 15.0 µg/mL. A similar dependence was observed in VASP assays for GSE 7.5 µg/mL and GSE 15 µg/mL. The effect on plasma coagulation tests was distinct only with GSE 15 µg/mL. All of the thromboelastometry variables were statistically significant with 15.0 µg/mL GSE concentration. Our results show, for the first time, the multi-potential effect of grape seed extract on coagulation systems, and clearly suggest that grape seed extract could be considered a promising nutraceutical in the prevention of cardiovascular thrombotic events caused by different mechanisms.

scholarly journals Effect of Adding Different Levels of Grapes (Vitis vinifera L.) Seeds and Leaf Powder or their Extracts on Some Bone Characteristics and Total Ash Content in Broiler Chickens

2021 ◽  
Author(s):  
Rabia J. Abbas ◽  
Taha Hasheem Khauoon
Keyword(s):  
Broiler Chickens ◽  
Leaf Extract ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Ash Content ◽  
Vitis Vinifera L ◽  
Grape Seeds ◽  
Leaf Powder ◽  
Grape Leaf

Background: Some natural sources of polyphenols like grape seed, leaf or extracts, have many benefits in maintaining bone health in animals. This study aimed to investigate the efficacy of grape seeds, grape leaves powder, or their extracts on some bone characteristics and total ash content in broiler chickens. Methods: Three hundred and twenty four one-day-old broilers were allocated to nine treatments with three replicates containing 12 chicks each. Chicks were fed nine experimental diets for 35 days; as a control diet without supplementation (T1), control diets supplemented with 15 and 30 g/kg grape seeds powder (GSP) (T2, T3), 15 and 30 g/kg grape leaf powder (GLP) (T4, T5), grape seed extract (GSE) at levels 2 and 4 ml/l added in drinking water (T6, T7) and with grape leaf extract (GLE ) at levels 2 and 4 ml/l (T8 and T9), respectively. Result: Significant increase (p≤0.05) in the bone length was recorded with GSP (30 g/kg), GLP (15 g/kg), GSE or GLE and calcium percent with GLE as compared with control. Furthermore, significant improvement was recorded in predictive skeletal weight of broilers fed GSE (T6 α T7) as compared to the other treatments. The study concluded that the best results were achieved at the 2 or 4 ml/l of grape seed extract in improving femur length, predicted skeletal weight and from grape leaf extract in improving calcium percentage in broiler bone ash.

scholarly journals Effect of Time and Haemolysis on Prothrombin Time (PT) and Activated Partial Thromboplastin Time (APTT) Measurement on Blood Samples

2021 ◽  
Vol 11 (6-S) ◽  
pp. 114-122
Author(s):  
Tahia Jafar Abdo Alhakam Eshag ◽  
Maye M. Merghani ◽  
Nihad Elsadig Babiker
Keyword(s):  
Prothrombin Time ◽  
Partial Thromboplastin Time ◽  
Blood Clot ◽  
Activated Partial Thromboplastin Time ◽  
Medical Laboratory ◽  
Control Group ◽  
Case Group ◽  
Age And Gender ◽  
Blood Samples ◽  
And Gender

Background: Coagulation, also known as blood clotting, is the process by which blood convert from a liquid to a gel, forming a blood clot. It referred to haemostasis, the stopping of blood loss from a damaged vessel, followed by repair. Material and methods: This was cross sectional study conducted at the albawasla medical laboratory, Khartoum, Sudan during the period August to November, 2021 and to evaluate the effect of time and hemolysis on prothrombin time and activated partial thromboplastin time tests. 50 samples (case group) were collected from the patients attending police teaching hospital   and requested to the PT and APTT test in addition to that,50 apparently healthy donors with no history of any coagulation problems or any chronic disease were selected as control group. Three ml of venous blood samples were collected in container with Tri Sodium Citrate anticoagulant. The coagulation tests (PT and APTT) were performed using semiautomatic device (coagulometer machine MI). Results:  The result of this study revealed that; when compared the measurement of PT and APTT immediately and after one hour there was insignificant differences (p. v.>0.05).  also when compared the measurement of PT and APTT between hemolyzed and non-hemolyzed samples there was significant differences ( p. v.<0.05)  in addition when compared case and control for the PT and APTT immediately,  after one hour, hemolyzed and non-hemolyzed sample there was significant differences ( p. v.<0.05) except the APTT hemolyzed samples  and  insignificant differences with age and gender ( p. v.>0.05).  For the correlation there was significant correlation in the case group for the PT and APTT immediately, after one hour, and hemolyzed samples. Conclusion: In the cases group results showed insignificant differences in the results of PT and APTT between immediate sample and after 1 hour in and significant differences in the results of PT and APTT between hemolyzed and non-hemolyzed samples, also there was insignificant differences between age and gender, immediately, after one hr. and hemolyzed sample in PT and APTT. Keywords:  Homeostasis,  hemolyzed  sample, PT and APTT

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