Spermatotoxic Effects of Some Medicinal Plants (Carica papaya, Hibiscus rosa-sinensis and Ipomoea batatas) on Sperm Quality and Testicular Weight in Male African catfish (Clarias gariepinus)

Testicular morphology and sperm motility were evaluated in cultured Clarias gariepinus (n = 25) purposively assigned to five groups according to their age. The results showed that the testes were paired, elongated, dorso-ventrally flattened structures, situated in the caudal aspects of the body cavity. The mean length of both right and left testes increased linearly with age, being significantly (p < 0.05) higher at 6 months than at 4 and 5 months of age, and also significantly (p < 0.05) higher at 8 months than at 6 months of age, while the mean weight and organo-somatic index of the catfish testes increased linearly until 6 months of age, after which no significant (p > 0.05) increase in the testicular weight and organo-somatic index was observed. Unidirectional progressive movement of spermatozoa was detected in the milt of C. gariepinus at 6, 7 and 8 months of age, but sperm cells were non-motile at 4 and 5 months of age. Histological sections showed seminiferous lobules, whose germinal epithelia were characterized by many cysts enclosing clones of sperm cells. Each cyst enclosed a clone of sperm cells at an identical stage of spermatogenesis. Spermatids and spermatozoa were present in the lumen of the seminiferous lobule. The obtained results indicate that the morphology of the testes of C. gariepinus is similar to the testes of members of the order Siluriformes, but sexual maturity and production of motile spermatozoa may be achieved at 6 months of age in the African catfish.

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Dietary Effects of Almond (Prunus amygdalus dulcis) Seed Powder on the Reproductive Indices in Male African Catfish (Clarias gariepinus) Broodstock

Asian Journal of Fisheries and Aquatic Research ◽

10.9734/ajfar/2019/v5i230069 ◽

2019 ◽

pp. 1-11

Author(s):

Dada Adekunle Ayokanmi ◽

Elemuo Chioma Cynthia ◽

Oke Israel Opeyemi

Keyword(s):

Sperm Quality ◽

Gonadosomatic Index ◽

Clarias Gariepinus ◽

Control Treatment ◽

African Catfish ◽

Prunus Amygdalus ◽

Dietary Effects ◽

Percentage Survival ◽

Somatic Index ◽

Reproductive Indices

Aims: To examine the dietary effects of Prunus amygdalus dulcis seed meal on the sperm quality of male African Catfish (Clarias gariepinus). Place and Duration of Study: The experiment was carried out at the Department of Fisheries and Aquaculture Technology Teaching and Research Farm, The Federal University of Technology, Akure for a period of 70 days. Methodology: Five diets with crude protein of 40% were formulated with different inclusion levels of P. amygdalus dulcis seed powder. D1 (control) has 0 g of the powder, while D2, D3, D4 and D5 has 5, 10, 15 and 20 g of the P. amygdalus dulcis seed powder in 100 g of feed respectively. A total of 45 C. gariepinus were randomly distributed into 15 concrete tanks (2×2×1.5 m) at stocking density of 3 fish per tank and constant water level of 1 m was maintained in the experimental tanks. The fish were fed at 3% of body weight twice a day between 08.00-09.00 and 16.00-17.00 hours for a period of 70 days. At the end of the 70-day experiment, gonadosomatic index and reproductive indices such as milt volume, motility duration, spermatozoa count, percentage motility, percentage fertilization, percentage hatchability and percentage survival of fry were determined. The qualities of the milt were assessed by aid of microscope and by fertility tests. Histological examination of the testes of fish fed each of the diets was also carried out. Results: Fish fed experimental diets showed significantly improved gonado-somatic index and reproductive indices over the control treatment. Higher gonado-somatic index and reproductive indices were recorded for the fish fed diet of 20 g/kg P. amygdalus dulcis seed powder compared to other experimental diets. Conclusion: The results indicated that supplementing diets with P. amygdalus dulcis seed powder enhanced growth and improved gonadosomatic index, and reproductive indices of male C. gariepinus broodstocks which can be exploited in fish seeds production.

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Effects of Medicinal Plant (Kigelia africana) on Sperm quality of African catfish Clarias gariepinus (Burchel, 1822) Broodstock

Journal of Agricultural Science ◽

10.5539/jas.v2n1p193 ◽

2010 ◽

Vol 2 (1) ◽

Cited By ~ 3

Author(s):

Eunice Oluwayemisi Adeparusi ◽

Adekunle Ayokanmi Dada ◽

Olakunle Victor Alale

Keyword(s):

Medicinal Plant ◽

Sperm Quality ◽

Clarias Gariepinus ◽

African Catfish

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Dietary effects of two medicinal plants (Sesamum indicum) and (Croton zambesicus) on the reproductive indices in female African catfish (Clarias gariepinus) broodstock

The Egyptian Journal of Aquatic Research ◽

10.1016/j.ejar.2012.12.012 ◽

2012 ◽

Vol 38 (4) ◽

pp. 269-273 ◽

Cited By ~ 2

Author(s):

A.A. Dada ◽

E.O. Adeparusi

Keyword(s):

Medicinal Plants ◽

Clarias Gariepinus ◽

Sesamum Indicum ◽

African Catfish ◽

Dietary Effects ◽

Reproductive Indices

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Partial and total fish meal replacement by agricultural products in the diets improve sperm quality in African catfish (Clarias gariepinus)

Theriogenology ◽

10.1016/j.theriogenology.2011.07.032 ◽

2012 ◽

Vol 77 (1) ◽

pp. 184-194 ◽

Cited By ~ 16

Author(s):

L. Nyina-wamwiza ◽

S. Milla ◽

M.-A. Pierrard ◽

E. Rurangwa ◽

S.N.M. Mandiki ◽

...

Keyword(s):

Fish Meal ◽

Sperm Quality ◽

Clarias Gariepinus ◽

Agricultural Products ◽

African Catfish ◽

Meal Replacement ◽

Total Fish ◽

Fish Meal Replacement

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Toxicity Effects of Brown Dried Pawpaw (Carica papaya) Leaf Extract to Fingerlings of African Catfish Clarias gariepinus

Asian Journal of Fisheries and Aquatic Research ◽

10.9734/ajfar/2019/v4i330055 ◽

2019 ◽

pp. 1-8

Author(s):

A. H. Ishaku ◽

A. I. Jauro ◽

Y. M. Gadaka ◽

A. Yagana ◽

R. A. Mohammed ◽

...

Keyword(s):

Aqueous Extract ◽

Leaf Extract ◽

Carica Papaya ◽

Clarias Gariepinus ◽

White Blood Cells ◽

Haemoglobin Concentration ◽

Control Fish ◽

African Catfish ◽

Corpuscular Haemoglobin ◽

Mean Corpuscular Haemoglobin

The acute and sub-lethal bioassay of aqueous extract of fresh pawpaw (Carica papaya) leaf on Clarias gariepinus fingerlings was investigated. The experiment was carried out at Department of Fisheries Teaching and Research Fish Farm, Modibbo Adama University of Technology Yola. At 96h static bioassay, symptoms of toxicity in the fish indicated that aqueous extract of fresh pawpaw leaf caused sub-acute effects such as altering fish behavior. These behaviors include air gulping, erratic swimming, discoloration, loss of reflex and skin peeling. These behavioral alterations were time and concentration dependent. Exposure to aqueous extract of fresh pawpaw leaf caused decrease in packed cells volume (PCV), haemoglobin (Hb), and red blood cell (RBC), mean corpuscular haemoglobin concentration (MCHC) and an increase in the mean corpuscular haemoglobin (MCH) and mean corpuscular volume (MCV). It resulted in marked increase in white blood cells (WBC). Mortalities and LC50-96h values for Clarias gariepinus exposed to fresh pawpaw leaf extract was (10.9 ml/l). The mortality rates in extracts to Clarias gariepinus in sub-lethal exposure was lower than in acute concentrations. The growth rates were significantly reduced in fish exposed to sub-lethal concentrations of the fresh pawpaw leaf extract compared to the control fish (p<0.05).

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Effects of Velvet Bean (Mucuna pruriens) on Sperm Quality of African Catfish, Clarias gariepinus (Burchell, 1822) Broodstock

Journal of Fisheries and Aquatic Science ◽

10.3923/jfas.2011.655.661 ◽

2011 ◽

Vol 6 (6) ◽

pp. 655-661 ◽

Cited By ~ 2

Author(s):

A. Adekunle Dada ◽

Folasade D. Ogunduyile

Keyword(s):

Sperm Quality ◽

Clarias Gariepinus ◽

Mucuna Pruriens ◽

African Catfish ◽

Velvet Bean

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EFEKTIVITAS ANTIBAKTERI BAWANG PUTIH (Allium sativum L) SEBAGAI PENGAWET ALAMI PADA IKAN LELE DUMBO (Clarias gariephinus) SEGAR

Surya Medika: Jurnal Ilmiah Ilmu Keperawatan dan Ilmu Kesehatan Masyarakat ◽

10.32504/sm.v14i1.106 ◽

2019 ◽

Vol 14 (1) ◽

pp. 26

Author(s):

Dyah Anggraeni ◽

Nurlela Nurlela

Keyword(s):

Room Temperature ◽

Allium Sativum ◽

Research Result ◽

Clarias Gariepinus ◽

Storage Period ◽

The Body ◽

Garlic Extract ◽

African Catfish ◽

Natural Preservatives ◽

Colony Counter

Background: Natural preservatives are compounds produced by natural ingredients that can suppress bacterial growth and development. Natural preservatives are carried out because most of the preservatives circulating are chemicals and unsafe for the body. One of the natural preservatives is by using garlic extract (Allium sativum L). Objective: This study is aimed to determine the effectiveness of the antibacterial garlic (Allium sativum L) as a natural preservative in fresh African catfish (Clarias gariepinus). Method: This research used the Pour Plate iroculation method. African catfish (Clarias gariepinus) which is soaked with garlic (Allium sativum L) with a concentration of 7%, 14% and 21% for 30 minutes, then the fish will be kept at room temperature with a storage period of 24 hours and 48 hours and calculated growth in bacterial numbers with the Colony counter. Result: Based on the research result, it was found that garlic extract (Allium sativum L) can obstruct the effectiveness of antibacterial in African catfish (Clarias gariepinus) at a concentration of 14%.

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PEMBEKUAN SEMEN LELE DUMBO (Clarias Gariepinus BURCHELL 1822) SEBAGAI MODEL KRIOPRESERVASI SEMEN IKAN

Jurnal Natural ◽

10.30862/jn.v9i1.778 ◽

2010 ◽

Vol 9 (1) ◽

Author(s):

Lutfi Lutfi

Keyword(s):

Liquid Nitrogen ◽

Clarias Gariepinus ◽

African Catfish ◽

Nitrogen Vapor ◽

Dmso Concentration ◽

Freezing Temperatures ◽

Vapor Liquid

Effect of four types of diluentss and four concentration of DMSO (5%, 10%, 15% and 20%) against the motility of African catfish sperm were evaluated after storage at freezing temperatures. The steps in preparation the 16 treatments combination of the diluents are preparation of diluents, mixing diluents with DMSO, packing of semen into 0.3-ml straw, equilibration of semen at 4 °C for 30 minutes, freezing of semen in nitrogen vapor liquid at a height of 6.5 cm for 10 minutes, and subsequently storage of semen in liquid nitrogen (-196 0C) for further analysis of post-thawing motility (PTM). The result showed that the highest level of motility of spermatozoa was in treatment P1D15 (45.7 ± 4.3%) and the lowest was in treatment P2D20 (14.5 ± 13.2%). The best diluent in this observation was diluents containing NaCl, KCl, CaCl2 and NaHCO3. The best concentration was DMSO 15%. While the best interaction between the concentration of DMSO diluents is P1D15 treatments containing NaCl, KCl, CaCl 2 and NaHCO3 with a combination of 15% DMSO concentration. The conclusion of the research is that diluents containing NaCl, KCl, CaCl2 and NaHCO3 with a combination of 15% DMSO concentration can be used in cryopreservation of African catfish semen.

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