Antagonistic Compatibility of Streptomyces griseorubens, Gliocladium virens, and Trichoderma harzianum Againts Fusarium oxysporum Cause of Tomato Wilt Deseases

2015 ◽  
Vol 5 (2) ◽  
pp. 82-89
Author(s):  
Penta Suryaminarsih ◽  
Kusriningrum ◽  
Ni'matuzaroh ◽  
Tini Surtiningsih
Agrivet ◽  
2021 ◽  
Vol 26 (2) ◽  
Author(s):  
Wiwit Rahayu ◽  
Rukmowati Brotodjojo ◽  
Nurngaini Nurngaini

Produksi tanaman tomat mempunyai kendala yang sering terjadi yaitu serangan penyakit layu Fusarium. Tujuan penelitian adalah mengetahui efektivitas dari jamur agensia hayati Trichoderma harzianum dan Gliocladium virens pada penyalutan benih tomat untuk pencegahan penyakit layu Fusarium. Penelitian dilaksanakan pada bulan Februari-Mei 2020 di Laboratorium Pengamatan Hama dan Penyakit Tanaman (LPHPT), Pandak, Bantul, DIY. Penelitian dilakukan menggunakan uji in vitro dan in vivo yang disusun menggunakan Rancangan Acak Lengkap (RAL) dengan 7 perlakuan dan 4 ulangan yaitu kontrol tanpa perlakuan, Fusarium, Trichoderma harzianum, Gliocladium virens, Fusarium + T.harzianum, Fusarium + G.virens dan kombinasi Fusarium +T.harzianum+ G.virens. Data dianalisis dengan menggunakan Analysis of Variance (ANOVA) pada taraf α=5% dan untuk mengetahui beda nyata antar perlakuan maka dilanjutkan uji Duncan’s Multiple Range Test (DMRT) taraf α=5%. Hasil penelitian menunjukkan perlakuan kombinasi T.harzianum+ G.virens efektif dalam menekan penyakit layu Fusarium. Uji in vitro menunjukkan bahwa Trichoderma harzianum, Gliocladium virens, atau kombinasi Fusarium +T.harzianum+ G.virens dapat menghambat pertumbuhan jamur F.oxysporum. Uji in vivo menunjukkan kombinasi T.harzianum+ G.virens menghasilkan indeks vigor, tinggi tanaman, jumlah daun, bobot segar akar, bobot kering akar nyata paling tinggi dan persen tanaman yang terserang layu Fusarium nyata paling rendah.


2018 ◽  
Vol 3 (2) ◽  
pp. 117-127
Author(s):  
Rizka Musfirah ◽  
Rina Sriwati ◽  
Tjut Chamzurni

Abstrak. Tomat (Solanum lycopersicum) merupakan salah satu komoditas pertanian yang ditanam secara luas di seluruh dunia, termasuk di Indonesia, karena memiliki rasa yang khas dan enak, juga memiliki nilai gizi seperti sumber vitamin A dan C yang sangat baik. Produksi tomat mengalami penurunan setiap tahun, salah satunya diakibatkan oleh organisme penganggu tanaman (OPT) yaitu patogen Fusarium oxysporum sehingga perlu dilakukan pengendalian hayati yaitu menggunakan Trichoderma harzianum dalam bentuk formulasi pelet yang praktis, efektif, dan efesien. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) non faktorial yang terdiri dari 6 perlakuan dengan 3 ulangan, setiap perlakuan terdiri dari 10 unit bibit tomat. Penelitian ini terdiri dari 6 perlakuan yaitu perlakuan A (masa simpan pelet T. harzianum  4 minggu), B (masa simpan pelet T. harzianum 3 minggu), C (masa simpan pelet T. harzianum 2 minggu), D (masa simpan pelet T. harzianum 1 minggu), E (masa simpan pelet T. harzianum 0 minggu), F (tanpa perlakuan pelet T. harzianum). Peubah yang diamati yaitu pre-emergence damping off, post-emergence damping off, masa inkubasi, persentase tanaman layu, tinggi tanaman, dan jumlah daun. Hasil penelitian menunjukkan bahwa pelet T. harzianum yang disimpan 4 minggu efektif dalam menghambat perkembangan penyakit layu fusarium seperti menunda masa inkubasi sampai 7 HSI, menekan pre-emergence damping off sampai 90%, post-emergence damping off 92,95%, serta mampu meningkatkan tinggi tanaman sampai 19,63 cm dan meningkatkan jumlah daun rata-rata 7 helai pada 35 HSI. (Storing Period of Trichoderma harzianum Pellets and its ability to Inhibit the development of Fusarium Wilt Disease on Tomato Seeds)Abstract. Tomato (Solanum lycopersicum) is one of the most widely grown commodities in the world, including Indonesia. It has a distinctively good taste and many nutritional value such as vitamin A and C. However, tomato production has decreased every year. One of the main cause is the attacks by pathogens, named Fusarium oxysporum. A Biological control is necessary and the use of Trichoderma harzianum in the form of pellets is recommended because of its effectiveness, efficiency and practical use. This research used a Completely Randomized Design (RAL) non-factorial consisted of 6 treatments with 3 replications, each treatment consisted of 10 units of tomato seedlings. The 6 treatments are named as treatment A (T. harzianum pellet saving 4 weeks), B (T. harzianum pellet saving period 3 weeks), C (shelf life of 2 weeks T. harzianum pellet), D (shelf life of pellet T harzianum 1 week), E (shelf life of pellet T. harzianum 0 weeks), and F (without T. harzianum pellet treatment). The variables observed in this study are pre-emergence damping off, post-emergence damping off, incubation period, the percentage of wilted plants, plant height, and the number of leaves. The results showed that pellets of T. harzianum stored 4 weeks effectively inhibiting the development of fusarium wilt disease such as delaying incubation period up to 7 HSI (Days After Incubation), suppressing the pre-emergence damping off up to 90% and post-emergence damping off to 92.95%, also able to increase the plant height up to 19.63 cm and increase the average leaf number of 7 strands at 35 HSI.


2020 ◽  
Vol 3 (2) ◽  
pp. 70-79
Author(s):  
Frara Efeseli Fakhdian ◽  
Abduh Ulim ◽  
Tjut Chamzurni

Abstrak:Cabai merah (Capsicum annum L.) merupakan salah satu komoditas hortikultura unggulan terbesar dari golongan sayur-sayuran karena memiliki harga jual yang tinggi. Disamping itu selain harga yang tinggi, di lapangan cabai merah sering  mengalami gangguan dari mulai benih disemai sampai tanaman cabai menghasilkan, hal ini disebabkan penyakit tular tanah atau pun terbawa benih. Salah satu penyakit yang memprihatinkan pada tanaman cabai merah adalah penyakit layu yang disebabkan oleh jamur Fusarium  oxysporum f.sp capsici,penyakit ini sering merugikan para petani, jika tanaman sudah terserang oleh penyakitini,  kerugian bisa mencapai 80 % pada tanaman cabai merah. Salah satu alternatif  yang digunakan dalam Pengendalian Hama dan Penyakit terpadu (PHPT) dengan menggunakan agensia Trichoderma formulasi pelet. Penelitian ini bertujuan agar mengetahui keefektifan Trichoderma  Formulasi pelet dalam menekan penyakit Layu Fusarium  yang disebabkan oleh Jamur Fusarium  oxysporum f.sp capsici. Penelitian ini telah dilaksanakan di laboratorium Penyakit Tumbuhan Program Studi Proteksi Tanaman dan Rumah Kasa Kebun Percobaan Fakultas Pertanian Unsyiah. Penelitian ini dimulai sejak bulan Agustus sampai Oktober 2017. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola non faktorial dengan 9 perlakuan dan 3 ulangan setiap perlakuan terdiri dari 5 unit polibag sehingga terdapat 135 unit polibag. Peubah yang diamati meliputi masa inkubasi, tinggi tanaman, jumlah daun dan persentase serangan.Hasil penelitian menunjukkan bahwa aplikasi pelet Trichoderma harzianum 2,5 g/polibagdan Trichoderma virens 2 g/polibag mampu memperlama masa inkubasi (Tidak terdapat gejala). Aplikasi pelet  T. virens 1,5 g polibag dapat meningkatkan tinggi tanaman cabai merah sampai 16, 80 cm. Aplikasi pelet T. harzianum  1,5 g/polibag dapat mamacu pertumbuhan  jumlah daun sebesar 9,07 helai. Aplikasi pelet T. harzianum 2,5 g/polibag dan T. virens formulasi pelet 2 g/polibag mampu menekan persentase serangan jamur Fusarium  oxysporum f.sp capsici sebesar 13,68 % pada 35 hari setelah tanam.Combination of Several Doses and Species of Trichoderma Pellet Formulations In Suppressing Development Fungus of fusarium oxysporum f.sp capsici in Red Chili Breeding (Capsicum annum L.)Abstract : a red chili (Capsicum annum L.) is one of the largest superior horticultural commodity of vegetables, because it has a high selling price. Although it has high price, but in the field red chilli often disturbance from the seed sowing until the pepper plants produce, it is cause by soil contagion or even carried by seed. One of the most common diseases in red chili plants is the wilting disease cause of the fungus Fusarium oxysporum f.sp capsici. It often detrimental of the farmers if the plants have been attacked by Fusarium wilt disease, the loss could reach out about 80% in red chili plants. One of the alternative to Integrated Pest and Disease Control (PHPT) is using the Trichoderma pellet formulation agent. The reasearch purpose to determine of the effectiveness Trichoderma Formulation of pellets in suppressing Fusarium Lung disease caused by Fusarium oxysporum f.sp capsici. The research has been conducted in Plant Disease Laboratory of Plant Protection Study Program and home srceen Experimental Garden of Agriculture Faculty Unsyiah. The research was started since August to October 2017. The research uses Completely Randomized Design (RAL) non factorial pattern with 9 treatments and 3 replications each treatment consists of 5 units of polybags so that there are 135 units of polybags. The variable observed included incubation period, plant height, number of leaves and percentage of attacks. Results of the research showed that the application of Trichoderma harzianum pellet 2.5 g / polybag and Trichoderma virens 2 g / polybag was able to prolong the incubation period. Application of T. virens pellet 1.5 g polybags can increase the height of red pepper plants up to 16, 80 cm. Application of pellets T. harzianum 1.5 g / polybags can spure growth of leaves amounted to 9.07 strands. The application of T. harzianum pellet 2.5 g / polybag and T. virens pellet 2 g / polybag formulation was able to suppress the percentage of Fusarium oxysporum f.sp capsici attack by 13.68% at 35 days after planting.


Pathogens ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 43 ◽  
Author(s):  
Arfe Castillo ◽  
Cecirly Puig ◽  
Christian Cumagun

Philippine banana is currently threatened by Fusarium oxysporum f. sp. cubense Tropical Race 4 (FocR4). This study investigated the use of Trichoderma harzianum pre-treated with Glomus spp, as a means of managing Fusarium wilt on young ‘Lakatan’ banana seedlings. Results showed that Glomus applied basally significantly improved banana seedling growth with increased increment in plant height and pseudostem diameter and heavier root weight. The application of Glomus spp. alone offered 100% protection to the ‘Lakatan’ seedlings against FocR4 as indicated by the absence of the wilting symptom. A combination of T. harzianum and Glomus spp. also gave significant effect against Fusarium wilt through delayed disease progression in the seedlings but was not synergistic. Competitive effects were suspected when application of the two biological control agents on banana roots was done simultaneously.


2005 ◽  
Vol 83 (2) ◽  
pp. 73-87 ◽  
Author(s):  
J. Caron ◽  
L. Laverdière ◽  
P.O. Thibodeau ◽  
R.R. Bélanger

Le potentiel antagoniste du biofongicide à base de Trichoderma harzianum MAUL-20, isolé au Québec, a été testé contre cinq agents telluriques phytopathogènes(Fusarium oxysporum f. sp. radicis-lycopersici (FORL), Pythium ultimum, Rhizoctonia solani, Sclerotinia sclerotiorum et Verticillium dahliae) du concombre et de la tomate de serre. Le biofongicide a démontré une efficacité contre P. ultimum et R. solani chez le concombre et la tomate et contre FORL chez la tomate. De plus, T. harzianum MAUL-20 a eu un effet stimulant sur le développement des plants de concombre lorsque cultivés, sans agents pathogènes, dans un substrat organique alimenté du biofongicide. L'efficacité de T. harzianum MAUL-20 a été comparée à celle du biofongicide américain Rootshield™ (Trichoderma harzianum KRL-AG2) et le premier a démontré une activité antagoniste égale ou supérieure à celle de Rootshield™.


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