Antagonistic potential of Trichoderma harzianum and Azadirachta indica against Fusarium oxysporum f. sp. capsici for the management of chilli wilt

Antagonistic potentials of seven rhizoshere soil fungi viz., Aspergillus flavus Link., A. fumigatus Fresen., A. niger Tiegh., A. terreus Thom., Penicillium sp., Trichoderma harzianum Refat. and T. viride Pers. were tested against the Fusarium oxysporum Schlecht. and F. solani (Mart.) Sacc. – the two pathogens, isolated from wilted roots of brinjal plants. Out of seven soil fungi Trichoderma harzianum was found most effective to control the growth of both the test pathogens. More than 50% inhibition of the test pathogens were observed with T. viride, Aspergillus niger and A. fumigatus in colony interaction. The maximum inhibition of radial growth of the test pathogens was observed with volatile metabolites and cell free culture filtrates of Trichoderma harzianum. This fungus may be exploited commercially to control the disease. Dhaka Univ. J. Biol. Sci. 24(2): 137-145, 2015 (July)

Download Full-text

ANTAGONISTIC POTENTIAL OF TRICHODERMA HARZIANUM AGAINST FUSARIUM OXYSPORUM F. SP. CUBENSE ASSOCIATED WITH PANAMA WILT OF BANANA

Pakistan Journal of Phytopathology ◽

10.33866/phytopathol.029.01.0299 ◽

2017 ◽

Vol 29 (1) ◽

pp. 111

Author(s):

Babar Khan ◽

Zuniara Akash ◽

Shahzad Asad ◽

Nazir Javed ◽

Nasir A. Rajput ◽

...

Keyword(s):

Fusarium Oxysporum ◽

Trichoderma Harzianum ◽

Vascular System ◽

Biological Control Agent ◽

Colony Growth ◽

Research Area ◽

Control Agent ◽

Research Centre ◽

Antagonistic Potential

Fusarium oxysporum f. sp. cubense is a soil loving pathogen of banana that can cause distortion of vascular system. It is responsible for the disease Fusarium wilt of bananas also renowned as panama wilt disease that has responsible of immense losses in the banana industry worldwide. In this study, diseased samples were collected from rhizosphere of banana plants in the research area of National Agriculture Research Centre (NARC), Islamabad and cultured on PDA to isolate pathogenic strains of F. oxysporium f. sp. cubense. Antagonistic potential of Trichoderma harzianum against F. oxysporum f. sp. cubense was evaluated under controlled conditions. T. harzianum produced up to 75.5% inhibition of colony growth of the pathogen followed by incubation for 72 h at 28+2°C in vitro. In pot culture T. harzianum considerably reduced disease severity. It proves that it is a potential biological control agent against banana wilt pathogen.

Download Full-text

Antagonistic Potential of Different Isolates of Trichoderma against Fusarium oxysporum, Rhizoctonia solani, and Botrytis cinerea

European Journal of Experimental Biology ◽

10.21767/2248-9215.100053 ◽

2018 ◽

Vol 08 (02) ◽

Cited By ~ 2

Author(s):

Estifanos Tsegaye Redda ◽

Jing Ma ◽

Jie Mei ◽

Mei Li ◽

Beilei Wu ◽

...

Keyword(s):

Rhizoctonia Solani ◽

Fusarium Oxysporum ◽

Botrytis Cinerea ◽

Antagonistic Potential

Download Full-text

Efeito do extrato hidroalcoólico e do óleo de nim (Azadirachta indica) sobre o fungo Fusarium oxysporum f. sp. vasinfectum e na resistência induzida de quiabeiros à fusariose

Research Society and Development ◽

10.33448/rsd-v10i2.12357 ◽

2021 ◽

Vol 10 (2) ◽

pp. e7110212357

Author(s):

Thiago Anchieta de Melo ◽

Ilka Márcia Ribeiro de Souza Serra ◽

Ingrid Tayane Vieira da Silva do Nascimento

Keyword(s):

Fusarium Oxysporum ◽

Azadirachta Indica ◽

Guaiacol Peroxidase

O objetivo deste trabalho foi verificar o efeito do extrato hidroalcoólico e do óleo de nim sobre o crescimento micelial, esporulação, germinação e adesão de conídios do fungo F. oxysporum f. sp. vasinfectum e avaliar o efeito desses produtos na indução de resistência em quiabeiros tratados e, posteriormente, inoculados com o fungo, por meio da atividade de enzimas antioxidantes relacionadas com processos de defesa vegetal. Os resultados evidenciaram que tanto o extrato quanto o óleo de nim foram eficientes em inibir o crescimento micelial e esporulação do fungo. Além disso, houve inibição da germinação e fixação de conídios produzidos na presença dos produtos. O extrato e o óleo de nim reduziram a severidade da doença nos quiabeiros, medida pelo índice de infecção da doença. Além disso, incrementos significativos das enzimas guaiacol-peroxidase, polifenoloxidase, superóxido-dismutase e catalase foram observados nos quiabeiros tratados com os produtos, sendo estes aumentos, diretamente proporcionais aos aumentos das concentrações utilizadas. Assim, tanto o extrato quanto o óleo de nim têm efeitos diretos sobre o desenvolvimento do fungo F. oxysporum f. sp. vasinfectum e induzem resistência em quiabeiros a partir da formação de espécies reativas de oxigênio (EROs).

Download Full-text

Uji masa simpan pelet Trichoderma harzianum dan kemampuannya dalam menghambat perkembangan penyakit Layu Fusarium pada bibit tomat.

Jurnal Ilmiah Mahasiswa Pertanian ◽

10.17969/jimfp.v3i2.7439 ◽

2018 ◽

Vol 3 (2) ◽

pp. 117-127

Author(s):

Rizka Musfirah ◽

Rina Sriwati ◽

Tjut Chamzurni

Keyword(s):

Vitamin A ◽

Shelf Life ◽

Fusarium Oxysporum ◽

Solanum Lycopersicum ◽

Plant Height ◽

Incubation Period ◽

Fusarium Wilt ◽

Trichoderma Harzianum ◽

Wilt Disease ◽

Damping Off

Abstrak. Tomat (Solanum lycopersicum) merupakan salah satu komoditas pertanian yang ditanam secara luas di seluruh dunia, termasuk di Indonesia, karena memiliki rasa yang khas dan enak, juga memiliki nilai gizi seperti sumber vitamin A dan C yang sangat baik. Produksi tomat mengalami penurunan setiap tahun, salah satunya diakibatkan oleh organisme penganggu tanaman (OPT) yaitu patogen Fusarium oxysporum sehingga perlu dilakukan pengendalian hayati yaitu menggunakan Trichoderma harzianum dalam bentuk formulasi pelet yang praktis, efektif, dan efesien. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) non faktorial yang terdiri dari 6 perlakuan dengan 3 ulangan, setiap perlakuan terdiri dari 10 unit bibit tomat. Penelitian ini terdiri dari 6 perlakuan yaitu perlakuan A (masa simpan pelet T. harzianum 4 minggu), B (masa simpan pelet T. harzianum 3 minggu), C (masa simpan pelet T. harzianum 2 minggu), D (masa simpan pelet T. harzianum 1 minggu), E (masa simpan pelet T. harzianum 0 minggu), F (tanpa perlakuan pelet T. harzianum). Peubah yang diamati yaitu pre-emergence damping off, post-emergence damping off, masa inkubasi, persentase tanaman layu, tinggi tanaman, dan jumlah daun. Hasil penelitian menunjukkan bahwa pelet T. harzianum yang disimpan 4 minggu efektif dalam menghambat perkembangan penyakit layu fusarium seperti menunda masa inkubasi sampai 7 HSI, menekan pre-emergence damping off sampai 90%, post-emergence damping off 92,95%, serta mampu meningkatkan tinggi tanaman sampai 19,63 cm dan meningkatkan jumlah daun rata-rata 7 helai pada 35 HSI. (Storing Period of Trichoderma harzianum Pellets and its ability to Inhibit the development of Fusarium Wilt Disease on Tomato Seeds)Abstract. Tomato (Solanum lycopersicum) is one of the most widely grown commodities in the world, including Indonesia. It has a distinctively good taste and many nutritional value such as vitamin A and C. However, tomato production has decreased every year. One of the main cause is the attacks by pathogens, named Fusarium oxysporum. A Biological control is necessary and the use of Trichoderma harzianum in the form of pellets is recommended because of its effectiveness, efficiency and practical use. This research used a Completely Randomized Design (RAL) non-factorial consisted of 6 treatments with 3 replications, each treatment consisted of 10 units of tomato seedlings. The 6 treatments are named as treatment A (T. harzianum pellet saving 4 weeks), B (T. harzianum pellet saving period 3 weeks), C (shelf life of 2 weeks T. harzianum pellet), D (shelf life of pellet T harzianum 1 week), E (shelf life of pellet T. harzianum 0 weeks), and F (without T. harzianum pellet treatment). The variables observed in this study are pre-emergence damping off, post-emergence damping off, incubation period, the percentage of wilted plants, plant height, and the number of leaves. The results showed that pellets of T. harzianum stored 4 weeks effectively inhibiting the development of fusarium wilt disease such as delaying incubation period up to 7 HSI (Days After Incubation), suppressing the pre-emergence damping off up to 90% and post-emergence damping off to 92.95%, also able to increase the plant height up to 19.63 cm and increase the average leaf number of 7 strands at 35 HSI.

Download Full-text

Kombinasi Beberapa Dosis dan Spesies Trichoderma Formulasi Pelet dalam Menekan Perkembangan Jamur Fusarium oxyporum f.sp capsici di Pembibitan Cabai Merah ( Capsicum annum L.)

Jurnal Ilmiah Mahasiswa Pertanian ◽

10.17969/jimfp.v3i2.7448 ◽

2020 ◽

Vol 3 (2) ◽

pp. 70-79

Author(s):

Frara Efeseli Fakhdian ◽

Abduh Ulim ◽

Tjut Chamzurni

Keyword(s):

Fusarium Oxysporum ◽

Incubation Period ◽

Trichoderma Harzianum ◽

Plant Protection ◽

High Price ◽

Selling Price ◽

Trichoderma Virens ◽

Study Program ◽

Capsicum Annum ◽

Pepper Plants

Abstrak:Cabai merah (Capsicum annum L.) merupakan salah satu komoditas hortikultura unggulan terbesar dari golongan sayur-sayuran karena memiliki harga jual yang tinggi. Disamping itu selain harga yang tinggi, di lapangan cabai merah sering mengalami gangguan dari mulai benih disemai sampai tanaman cabai menghasilkan, hal ini disebabkan penyakit tular tanah atau pun terbawa benih. Salah satu penyakit yang memprihatinkan pada tanaman cabai merah adalah penyakit layu yang disebabkan oleh jamur Fusarium oxysporum f.sp capsici,penyakit ini sering merugikan para petani, jika tanaman sudah terserang oleh penyakitini, kerugian bisa mencapai 80 % pada tanaman cabai merah. Salah satu alternatif yang digunakan dalam Pengendalian Hama dan Penyakit terpadu (PHPT) dengan menggunakan agensia Trichoderma formulasi pelet. Penelitian ini bertujuan agar mengetahui keefektifan Trichoderma Formulasi pelet dalam menekan penyakit Layu Fusarium yang disebabkan oleh Jamur Fusarium oxysporum f.sp capsici. Penelitian ini telah dilaksanakan di laboratorium Penyakit Tumbuhan Program Studi Proteksi Tanaman dan Rumah Kasa Kebun Percobaan Fakultas Pertanian Unsyiah. Penelitian ini dimulai sejak bulan Agustus sampai Oktober 2017. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola non faktorial dengan 9 perlakuan dan 3 ulangan setiap perlakuan terdiri dari 5 unit polibag sehingga terdapat 135 unit polibag. Peubah yang diamati meliputi masa inkubasi, tinggi tanaman, jumlah daun dan persentase serangan.Hasil penelitian menunjukkan bahwa aplikasi pelet Trichoderma harzianum 2,5 g/polibagdan Trichoderma virens 2 g/polibag mampu memperlama masa inkubasi (Tidak terdapat gejala). Aplikasi pelet T. virens 1,5 g polibag dapat meningkatkan tinggi tanaman cabai merah sampai 16, 80 cm. Aplikasi pelet T. harzianum 1,5 g/polibag dapat mamacu pertumbuhan jumlah daun sebesar 9,07 helai. Aplikasi pelet T. harzianum 2,5 g/polibag dan T. virens formulasi pelet 2 g/polibag mampu menekan persentase serangan jamur Fusarium oxysporum f.sp capsici sebesar 13,68 % pada 35 hari setelah tanam.Combination of Several Doses and Species of Trichoderma Pellet Formulations In Suppressing Development Fungus of fusarium oxysporum f.sp capsici in Red Chili Breeding (Capsicum annum L.)Abstract : a red chili (Capsicum annum L.) is one of the largest superior horticultural commodity of vegetables, because it has a high selling price. Although it has high price, but in the field red chilli often disturbance from the seed sowing until the pepper plants produce, it is cause by soil contagion or even carried by seed. One of the most common diseases in red chili plants is the wilting disease cause of the fungus Fusarium oxysporum f.sp capsici. It often detrimental of the farmers if the plants have been attacked by Fusarium wilt disease, the loss could reach out about 80% in red chili plants. One of the alternative to Integrated Pest and Disease Control (PHPT) is using the Trichoderma pellet formulation agent. The reasearch purpose to determine of the effectiveness Trichoderma Formulation of pellets in suppressing Fusarium Lung disease caused by Fusarium oxysporum f.sp capsici. The research has been conducted in Plant Disease Laboratory of Plant Protection Study Program and home srceen Experimental Garden of Agriculture Faculty Unsyiah. The research was started since August to October 2017. The research uses Completely Randomized Design (RAL) non factorial pattern with 9 treatments and 3 replications each treatment consists of 5 units of polybags so that there are 135 units of polybags. The variable observed included incubation period, plant height, number of leaves and percentage of attacks. Results of the research showed that the application of Trichoderma harzianum pellet 2.5 g / polybag and Trichoderma virens 2 g / polybag was able to prolong the incubation period. Application of T. virens pellet 1.5 g polybags can increase the height of red pepper plants up to 16, 80 cm. Application of pellets T. harzianum 1.5 g / polybags can spure growth of leaves amounted to 9.07 strands. The application of T. harzianum pellet 2.5 g / polybag and T. virens pellet 2 g / polybag formulation was able to suppress the percentage of Fusarium oxysporum f.sp capsici attack by 13.68% at 35 days after planting.

Download Full-text

Non-Synergistic Effect of Trichoderma harzianum and Glomus spp. in Reducing Infection of Fusarium Wilt in Banana

Pathogens ◽

10.3390/pathogens8020043 ◽

2019 ◽

Vol 8 (2) ◽

pp. 43 ◽

Cited By ~ 2

Author(s):

Arfe Castillo ◽

Cecirly Puig ◽

Christian Cumagun

Keyword(s):

Biological Control ◽

Synergistic Effect ◽

Fusarium Oxysporum ◽

Fusarium Wilt ◽

Trichoderma Harzianum ◽

Root Weight ◽

Biological Control Agents ◽

Competitive Effects ◽

Glomus Spp ◽

Race 4

Philippine banana is currently threatened by Fusarium oxysporum f. sp. cubense Tropical Race 4 (FocR4). This study investigated the use of Trichoderma harzianum pre-treated with Glomus spp, as a means of managing Fusarium wilt on young ‘Lakatan’ banana seedlings. Results showed that Glomus applied basally significantly improved banana seedling growth with increased increment in plant height and pseudostem diameter and heavier root weight. The application of Glomus spp. alone offered 100% protection to the ‘Lakatan’ seedlings against FocR4 as indicated by the absence of the wilting symptom. A combination of T. harzianum and Glomus spp. also gave significant effect against Fusarium wilt through delayed disease progression in the seedlings but was not synergistic. Competitive effects were suspected when application of the two biological control agents on banana roots was done simultaneously.

Download Full-text

Utilisation d'une souche indigène de Trichoderma harzianum contre cinq agents pathogènes chez le concombre et la tomate de serre au Québec

Phytoprotection ◽

10.7202/706230ar ◽

2005 ◽

Vol 83 (2) ◽

pp. 73-87 ◽

Cited By ~ 2

Author(s):

J. Caron ◽

L. Laverdière ◽

P.O. Thibodeau ◽

R.R. Bélanger

Keyword(s):

Rhizoctonia Solani ◽

Fusarium Oxysporum ◽

Sclerotinia Sclerotiorum ◽

Verticillium Dahliae ◽

Trichoderma Harzianum ◽

Pythium Ultimum

Le potentiel antagoniste du biofongicide à base de Trichoderma harzianum MAUL-20, isolé au Québec, a été testé contre cinq agents telluriques phytopathogènes(Fusarium oxysporum f. sp. radicis-lycopersici (FORL), Pythium ultimum, Rhizoctonia solani, Sclerotinia sclerotiorum et Verticillium dahliae) du concombre et de la tomate de serre. Le biofongicide a démontré une efficacité contre P. ultimum et R. solani chez le concombre et la tomate et contre FORL chez la tomate. De plus, T. harzianum MAUL-20 a eu un effet stimulant sur le développement des plants de concombre lorsque cultivés, sans agents pathogènes, dans un substrat organique alimenté du biofongicide. L'efficacité de T. harzianum MAUL-20 a été comparée à celle du biofongicide américain Rootshield™ (Trichoderma harzianum KRL-AG2) et le premier a démontré une activité antagoniste égale ou supérieure à celle de Rootshield™.

Download Full-text