scholarly journals Antioxidant Property and Oral Glucose Tolerance of Stem Extract of Andrographis paniculata – Nees

2021 ◽  
pp. 21-28
Author(s):  
E. O. Awoyinka ◽  
A. O. Ogundare ◽  
T. T. Adebolu ◽  
Adeloye Adedeji Isaac
Keyword(s):  
Free Radicals ◽  
Glucose Tolerance ◽  
Antioxidant Property ◽  
Andrographis Paniculata ◽  
Albino Rats ◽  
Oral Glucose ◽  
Oral Glucose Tolerance ◽  
Stem Extract ◽  
Group 5 ◽  
Wistar Albino Rats

This research aims to investigate the antioxidant property and oral glucose tolerance of stem extract of Andrographis paniculata using standard methods. The antioxidant activity of the extracts were assessed using total phenol, total flavonoid, ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)), nitric oxide, GSH (gluthatione), super oxide, DPPH [1,1-di-phenyl-2-picryl hydrazyl] and ascorbic acid.  The oral glucose tolerance of methanol stem extract of A. paniculata was carried out using wistar albino rats. The Wistar albino rats were divided into six groups consisting of five rats each.  Group 1 was given water only, group two to group five were induced with 2g/kg of glucose. After 30 minutes, group 3 and 4 were treated with the doses of 15 mg/kg and 30 mg/kg of the extract while group 5 was treated with glibenclamide respectively. The blood glucose level of each group was monitored for 2hours at 30 minutes interval and compared with the control that was given water only. The result shows that the extracts were able to scavenge free radicals but methanol stem extract of A. paniculata performed better in scavenging free radicals as compared to others. The blood sugar levels of the rats treated with doses of 15 and 30 mg/kg of methanol stem extract of A. paniculata were significantly lower than the group of rats that were fed with only glucose and the group of rats treated with glibenclamide. The glucose-lowering efficiency of the extract was between 60-120 minutes. Findings from this present study revealed that A. paniculata is a potential plant that can scavenge free radicals and also possess hypoglycemic activity.

scholarly journals Comparative Antioxidative and Antihyperglycemic Profiles of Pneumatophores of Two Mangrove Species Avicennia alba and Sonneratia apetala

2018 ◽  
Vol 17 (2) ◽  
pp. 205-211 ◽  
Author(s):  
Biswajit Biswas ◽  
Mimi Golder ◽  
Tannami Islam ◽  
Samir Kumar Sadhu
Keyword(s):  
Free Radicals ◽  
Glucose Tolerance ◽  
Oral Glucose Tolerance Test ◽  
Glucose Tolerance Test ◽  
Tolerance Test ◽  
Oral Glucose ◽  
Oral Glucose Tolerance ◽  
Mangrove Species ◽  
Sonneratia Apetala ◽  
Avicennia Alba

Ethanolic extracts of pneumatophores of two mangrove species- Avicennia alba and Sonneratia apetala were studied in vitro for antioxidant capacity by measuring their ability to scavenge free radicals and determining total phenolic, flavonoid and tannin contents. In vivo measurement of antihyperglycemic activity of extracts was done by oral glucose tolerance test. In considering the antioxidant activity, S. apetala extract showed superior IC50 (concentration of sample required to inhibit 50% of free radicals) value for scavenging DPPH radical (71.77 μg/ml), hydrogen peroxide radical (97.27 mg/l), hydroxyl radical (79.62 mg/l) and superoxide anion (108.89 mg/l). For A. alba, the values for the radical scavenging assays were much higher. In addition, total phenol, flavonoid and tannin content demonstrated by S. apetala were 204.03 mgGAE/g, 228.68 mgQE/g and 235.89 mgGAE/g whereas for A. alba, they were 65.52 mgGAE/g, 44 mgQE/g and 37.71 mgGAE/g, respectively. In oral glucose tolerance test, S. apetala reduced the blood glucose level to a higher extent than A. alba. So, S. apetala with higher amount of secondary metabolites (phenol, flavonoid, tannin) is a superior source of natural antioxidants and antihyperglycemics. Dhaka Univ. J. Pharm. Sci. 17(2): 205-211, 2018 (December)

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