A Review on In vitro Cell Culture Model for Bacterial Adhesion and Invasion: From Simple Monoculture to Co-Culture Human Intestinal Epithelium Model

Aim: This paper reviews the different in vitro models of human intestinal epithelium that have been utilized for studying the adhesion and invasion properties. Problem Statement: The cell adhesion and invasion are the key mechanisms of bacterial pathogenicity that determines their possible routes of transmission. Numerous investigations related to the adhesion and invasion ability of bacterial isolates have been reported on monoculture human intestinal cells. However, the use of monoculture cells has several major disadvantages, such as the inability to reproduce the complex structure that defines the intestine and the inability to accurately predict the mechanism of bacterial adhesion and invasion. Approach: Co-culture models of human intestine have been developed as an alternative to improve the monoculture epithelial cell for adhesion and invasion studies, which provide more flexibility and overcome some of the limitations Conclusion: With the use of diverse in vitro approach, it could provide thorough information on different ability of bacterial adhesion and invasion and it could help to clarify the intricacy of host-pathogen interactions that underpin bacterial pathogenesis.

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Use of hydrogel scaffolds to develop an in vitro 3D culture model of human intestinal epithelium

Acta Biomaterialia ◽

10.1016/j.actbio.2017.08.035 ◽

2017 ◽

Vol 62 ◽

pp. 128-143 ◽

Cited By ~ 18

Author(s):

R.H. Dosh ◽

A. Essa ◽

N. Jordan-Mahy ◽

C. Sammon ◽

C.L. Le Maitre

Keyword(s):

Intestinal Epithelium ◽

3D Culture ◽

Hydrogel Scaffolds ◽

Culture Model ◽

3D Culture Model ◽

Human Intestinal Epithelium

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In vitro models replicating the human intestinal epithelium for absorption and metabolism studies: A systematic review

Journal of Controlled Release ◽

10.1016/j.jconrel.2021.05.028 ◽

2021 ◽

Author(s):

Arianna Fedi ◽

Chiara Vitale ◽

Giulia Ponschin ◽

Seyoum Ayehunie ◽

Marco Fato ◽

...

Keyword(s):

Systematic Review ◽

Intestinal Epithelium ◽

In Vitro Models ◽

Human Intestinal Epithelium

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Bisphosphonates Reduce Smoking-Induced Osteoporotic-Like Alterations by Regulating RANKL/OPG in an Osteoblast and Osteoclast Co-Culture Model

International Journal of Molecular Sciences ◽

10.3390/ijms22010053 ◽

2020 ◽

Vol 22 (1) ◽

pp. 53

Author(s):

Sheng Zhu ◽

Victor Häussling ◽

Romina H. Aspera-Werz ◽

Tao Chen ◽

Bianca Braun ◽

...

Keyword(s):

Alternative Therapy ◽

Cigarette Smoke Extract ◽

Matrix Remodeling ◽

Nuclear Factor ◽

Bone Homeostasis ◽

Potential Mechanism ◽

Culture Model ◽

Receptor Activator ◽

Culture Models

Co-culture models have become mandatory for obtaining better insights into bone homeostasis, which relies on the balance between osteoblasts and osteoclasts. Cigarette smoking (CS) has been proven to increase the risk of osteoporosis; however, there is currently no proven treatment for osteoporosis in smokers excluding cessation. Bisphosphonates (BPs) are classical anti-osteoclastic drugs that are commonly used in examining the suitability of bone co-culture systems in vitro as well as to verify the response to osteoporotic stimuli. In the present study, we tested the effects of BPs on cigarette smoke extract (CSE)-affected cells in the co-culture of osteoblasts and osteoclasts. Our results showed that BPs were able to reduce CSE-induced osteoporotic alterations in the co-culture of osteoblasts and osteoclasts such as decreased matrix remodeling, enhanced osteoclast activation, and an up-regulated receptor activator of nuclear factor (NF)-kB-ligand (RANKL)/osteoprotegerin (OPG) ratio. In summary, BPs may be an effective alternative therapy for reversing osteoporotic alterations in smokers, and the potential mechanism is through modulation of the RANKL/OPG ratio.

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An integrated framework for quantifying immune-tumour interactions in a 3D co-culture model

Communications Biology ◽

10.1038/s42003-021-02296-7 ◽

2021 ◽

Vol 4 (1) ◽

Author(s):

Gheed Al-Hity ◽

FengWei Yang ◽

Eduard Campillo-Funollet ◽

Andrew E. Greenstein ◽

Hazel Hunt ◽

...

Keyword(s):

Immune System ◽

Immune Cell ◽

In Vitro Models ◽

Proof Of Concept ◽

Culture Model ◽

Spheroid Growth ◽

Confocal Images ◽

Cancer Spheroids

AbstractInvestigational in vitro models that reflect the complexity of the interaction between the immune system and tumours are limited and difficult to establish. Herein, we present a platform to study the tumour-immune interaction using a co-culture between cancer spheroids and activated immune cells. An algorithm was developed for analysis of confocal images of the co-culture to evaluate the following quantitatively; immune cell infiltration, spheroid roundness and spheroid growth. As a proof of concept, the effect of the glucocorticoid stress hormone, cortisol was tested on 66CL4 co-culture model. Results were comparable to 66CL4 syngeneic in vivo mouse model undergoing psychological stress. Furthermore, administration of glucocorticoid receptor antagonists demonstrated the use of this model to determine the effect of treatments on the immune-tumour interplay. In conclusion, we provide a method of quantifying the interaction between the immune system and cancer, which can become a screening tool in immunotherapy design.

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Responses of increasingly complex intestinal epithelium in vitro models to bacterial toll-like receptor agonists

Toxicology in Vitro ◽

10.1016/j.tiv.2021.105280 ◽

2021 ◽

pp. 105280

Author(s):

Menno Grouls ◽

Meike van der Zande ◽

Laura de Haan ◽

Hans Bouwmeester

Keyword(s):

Intestinal Epithelium ◽

In Vitro Models ◽

Toll Like Receptor ◽

Receptor Agonists

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Three-Dimensional In Vitro Hydro- and Cryogel-Based Cell-Culture Models for the Study of Breast-Cancer Metastasis to Bone

Cancers ◽

10.3390/cancers10090292 ◽

2018 ◽

Vol 10 (9) ◽

pp. 292 ◽

Cited By ~ 9

Author(s):

Laura Bray ◽

Constanze Secker ◽

Berline Murekatete ◽

Jana Sievers ◽

Marcus Binner ◽

...

Keyword(s):

Breast Cancer ◽

Cancer Metastasis ◽

Cell Function ◽

Three Dimensional ◽

Breast Cancer Metastasis ◽

In Vitro Models ◽

Cellular Migration ◽

Breast Tumor Tissue ◽

Culture Models

Bone is the most common site for breast-cancer invasion and metastasis, and it causes severe morbidity and mortality. A greater understanding of the mechanisms leading to bone-specific metastasis could improve therapeutic strategies and thus improve patient survival. While three-dimensional in vitro culture models provide valuable tools to investigate distinct heterocellular and environmental interactions, sophisticated organ-specific metastasis models are lacking. Previous models used to investigate breast-to-bone metastasis have relied on 2.5D or singular-scaffold methods, constraining the in situ mimicry of in vitro models. Glycosaminoglycan-based gels have demonstrated outstanding potential for tumor-engineering applications. Here, we developed advanced biphasic in vitro microenvironments that mimic breast-tumor tissue (MCF-7 and MDA-MB-231 in a hydrogel) spatially separated with a mineralized bone construct (human primary osteoblasts in a cryogel). These models allow distinct advantages over former models due to the ability to observe and manipulate cellular migration towards a bone construct. The gels allow for the binding of adhesion-mediating peptides and controlled release of signaling molecules. Moreover, mechanical and architectural properties can be tuned to manipulate cell function. These results demonstrate the utility of these biomimetic microenvironment models to investigate heterotypic cell–cell and cell–matrix communications in cancer migration to bone.

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Bottom-up self-assembly of heterotrimeric nanoparticles and their secondary Janus generations

Chemical Science ◽

10.1039/c9sc02961c ◽

2019 ◽

Vol 10 (44) ◽

pp. 10388-10394 ◽

Cited By ~ 5

Author(s):

Jianye Fu ◽

Zhengying Gu ◽

Yang Liu ◽

Jun Zhang ◽

Hao Song ◽

...

Keyword(s):

Silica Nanoparticles ◽

Intestinal Epithelium ◽

Self Assembly ◽

Bottom Up ◽

Epithelial Monolayer ◽

Cargo Transport ◽

Human Intestinal Epithelium ◽

Monolayer Model

Designed Janus silica nanoparticles can stimulate stronger phagocytosis and exhibit higher cargo transport across an in vitro epithelial monolayer model mimicking the human intestinal epithelium.

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Anti-inflammatory effects of dietary phenolic compounds in an in vitro model of inflamed human intestinal epithelium

Chemico-Biological Interactions ◽

10.1016/j.cbi.2010.08.007 ◽

2010 ◽

Vol 188 (3) ◽

pp. 659-667 ◽

Cited By ~ 105

Author(s):

Thérèse Sergent ◽

Neil Piront ◽

Julie Meurice ◽

Olivier Toussaint ◽

Yves-Jacques Schneider

Keyword(s):

Phenolic Compounds ◽

Intestinal Epithelium ◽

In Vitro Model ◽

Anti Inflammatory ◽

Vitro Model ◽

Human Intestinal Epithelium

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Cellular Composition and Differentiation Signaling in Chicken Small Intestinal Epithelium

Animals ◽

10.3390/ani9110870 ◽

2019 ◽

Vol 9 (11) ◽

pp. 870

Author(s):

Haihan Zhang ◽

Dongfeng Li ◽

Lingbin Liu ◽

Ling Xu ◽

Mo Zhu ◽

...

Keyword(s):

Intestinal Epithelium ◽

Intestinal Development ◽

Cell Culture Model ◽

Proliferation Zone ◽

Small Intestinal Epithelium ◽

Culture Model ◽

Different Types ◽

Development And Differentiation ◽

Small Intestinal

The small intestine plays an important role for animals to digest and absorb nutrients. The epithelial lining of the intestine develops from the embryonic endoderm of the embryo. The mature intestinal epithelium is composed of different types of functional epithelial cells that are derived from stem cells, which are located in the crypts. Chickens have been widely used as an animal model for researching vertebrate embryonic development. However, little is known about the molecular basis of development and differentiation within the chicken small intestinal epithelium. This review introduces processes of development and growth in the chicken gut, and compares the cellular characteristics and signaling pathways between chicken and mammals, including Notch and Wnt signaling that control the differentiation in the small intestinal epithelium. There is evidence that the chicken intestinal epithelium has a distinct cellular architecture and proliferation zone compared to mammals. The establishment of an in vitro cell culture model for chickens will provide a novel tool to explore molecular regulation of the chicken intestinal development and differentiation.

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