scholarly journals Antibacterial Activity of Bioflavonoid from Fruit Pulps of Acacia nilotica Willd

2019 ◽  
pp. 1-12
Author(s):  
O. P. Oladosu ◽  
N. R. Isu ◽  
I. M. Aboh ◽  
S. E. Okhale ◽  
A. T. Orishadipe ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Liquid Chromatography ◽  
Gallic Acid ◽  
High Performance ◽  
Acacia Nilotica ◽  
Multi Drug Resistance ◽  
Klebsiella Pneumonia ◽  
Methyl Gallate ◽  
Medicinal Uses ◽  
Test Organisms

The emergence of multi-drug resistance in bacteria has led to call for research and development of new leads as antibiotics from medicinal plants. Acacia nilotica (Linn) is a plant of multipurpose medicinal uses, three bioactive flavonoids (methyl gallate, gallic acid and catechin) were isolated from its fruit pulps through a bioassay guided fractionation technique and characterized based on High Performance Liquid Chromatography, Liquid Chromatography-Mass Spectra and Nuclear Magnetic Resonance spectra. Antibacterial activity of these compounds was determined by microplate tetrazolium dye assay of broth microdilution technique against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and clinical isolates of Salmonella typhi, Klebsiella pneumonia, Candida albicans and Bacillus subtilis. Catechin, methyl gallate and gallic acid at 19.5, 39 and 39 µg/ml respectively caused a significant bio-reduction in cells of test organisms. Time kill kinetic study of the extract shows that there was percentage of growth reduction in test organisms at 2, 4, 6, 8 and 12 hrs of contact. The extent and rate of killing of the organism by the extract at 2 x MIC followed the same trend as rate of killing was time dependent. Antibacterial effects of these compounds are within the breakpoint of control drug chloramphenicol and could serve as leads in new drug development.

scholarly journals In vitro activities of Acacia nilotica (L.) Delile bark fractions against Oral Bacteria, Glucosyltransferase and as antioxidant

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Ali Mahmoud Muddathir ◽  
Ebtihal Abdalla M. Mohieldin ◽  
Tohru Mitsunaga
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Gallic Acid ◽  
Crude Extract ◽  
Inhibitory Activity ◽  
Oral Bacteria ◽  
Acacia Nilotica ◽  
Assay Method ◽  
Medicinal Uses ◽  
Wide Range

Abstract Background Dental caries and periodontal disease are the most common chronic infectious oral diseases in the world. Acacia nilotica was commonly known in Sudan as Garad or Sunt has a wide range of medicinal uses. In the present study, antibacterial activity of oral bacteria (Streptococcus sobrinus and Porphyromonas gingivalis), inhibitory activity against glucosyltransferase (GTF) enzyme and antioxidant activity were assayed for methanolic crude extract of A. nilotica bark and its fractions. Methods Methanoilc crude extract of A. nilotica bark was applied to a Sephadex LH-20 column and eluted with methanol, aqueous methanol, and finally aqueous acetone to obtain four fractions (Fr1- Fr4). Furthermore, the crude extract and fractions were subjected to analytical high performance liquid chromatography (HPLC). The crude extract and its fractions were assayed for antibacterial activity against S. sobrinus and P. gingivalis using a microplate dilution assay method to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), as well as GTF inhibition and antioxidant activity using ABTS radical scavenging method. Results Fractions (Fr1 and Fr2) exhibited MIC values of 0.3 mg/ml against the P. gingivalis. Additionally, Fr2 displayed MBC value of 1 mg/ml against two types of bacteria. Fr4 showed an especially potent GTF inhibitory activity with IC50 value of 3.9 μg/ml. Fr1 displayed the best antioxidant activity with IC50 value of 1.8 μg/ml. The main compound in Fr1 was identified as gallic acid, and Fr2 was mostly a mixture of gallic acid and methyl gallate. Conclusions The results obtained in this study provide some scientific rationale and justify the use of this plant for the treatment of dental diseases in traditional medicine. A. nilotica bark, besides their antibacterial potentiality and GTF inhibitory activity, it may be used as adjuvant antioxidants in mouthwashes. Further studies in the future are required to identify the rest of the active compounds.

scholarly journals Modulation of Brain-Derived Neurotrophic Factor (BDNF) Signaling Pathway by Culinary Sage (Salvia officinalis L.)

2021 ◽  
Vol 22 (14) ◽  
pp. 7382
Author(s):  
Nancy Chiang ◽  
Shahla Ray ◽  
Jade Lomax ◽  
Sydney Goertzen ◽  
Slavko Komarnytsky ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Neurotrophic Factor ◽  
High Performance ◽  
Memory Function ◽  
Brain Derived Neurotrophic Factor ◽  
Salvia Officinalis ◽  
C6 Glioma Cells ◽  
Medicinal Uses ◽  
Bdnf Signaling ◽  
Salvia Officinalis L

Culinary sage (Salvia officinalis L.) is a common spice plant in the mint family (Lamiaceae) well known for its distinctive culinary and traditional medicinal uses. Sage tea has been used traditionally as a brain-enhancing tonic and extracts from sage have been reported to have both cognitive and memory enhancing effects. Brain-derived neurotrophic factor (BDNF) is an endogenous signaling molecule involved in cognition and memory function. In this study, activity-guided fractionation employing preparative reverse-phase high performance liquid chromatography (RP-HPLC) of culinary sage extracts led to the discovery of benzyl 6-O-β-D-apiofuranosyl-β-D-glucoside (B6AG) as a natural product that upregulates transcription of neurotrophic factors in C6 glioma cells. Purified B6AG showed a moderate dose response, with upregulation of BDNF and with EC50 at 6.46 μM. To better understand the natural variation in culinary sage, B6AG was quantitated in the leaves of several commercial varieties by liquid chromatography-mass spectrometry (LC-MS). The level of B6AG in dried culinary sage was found to range from 334 ± 14 to 698 ± 65 μg/g. This study provided a foundation for future investigations, including quantitative inquiries on the distribution of B6AG within the different plant organs, explorations in optimizing post-harvest practices, and aid in the development of sage varieties with elevated levels of B6AG.

scholarly journals QUANTITATIVE DETERMINATION OF PHENOL CARBOXYLIC ACIDS IN POMEGRANATE FRUIT PULP BY THE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION

2019 ◽  
Vol 12 (1) ◽  
pp. 548
Author(s):  
Ruben Ashotovich Pogosyan ◽  
Olga Vladimirovna Nesterova ◽  
Dmitry Olegovich Bokov ◽  
Irina Alexandrovna Samylina
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Quantitative Determination ◽  
Gallic Acid ◽  
Carboxylic Acids ◽  
High Performance ◽  
Fruit Pulp ◽  
Pomegranate Fruit

Objective: Pomegranate (Punica granatum L.) is a broadly used plant possessing a wide range of medicinal properties. In this research, we have mainly focused on the investigation of phenolic compounds of pomegranate fruit pulp (PFP).Methods: Fresh fruits of “Çəhrayı Gülöyşə,” “Kizil-anor,” and pomegranate varietal mixture were used as samples. High-performance liquid chromatography-ultraviolet (HPLC-UV) analysis of phenol carboxylic acids was performed with metal column Kromasil® C18 (4.6×250 mm, particle size 5 μm) and the acetonitrile-water-concentrated acid phosphoric system (400:600:5) under isocratic elution conditions (flow rate of 0.5 ml/min). Detection was carried out using a UV detector “GILSTON” UV/Visible model 151 at a wavelength of 280 nm.Results and Discussion: As a result of our research, we proposed chromatographic conditions for the separation of phenolic compounds, the conditions for sample preparation of PFP. Procedure for determination of phenolic carboxylic acids total content in terms of gallic acid by HPLC-UV method was developed. According to the obtained data, the content of phenolic carboxylic acids should be at least 0.7%.Conclusion: Procedure for the quantitative determination of gallic acid using the HPLC-UV method was developed. This method which can be used in the standardization of new medicinal plant raw materials - PFP, as well as extract preparations based on it in the future.

scholarly journals QUANTITATIVE DETERMINATION OF PHENOL CARBOXYLIC ACIDS IN POMEGRANATE FRUIT PULP BY THE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION

2019 ◽  
Vol 12 (1) ◽  
pp. 548
Author(s):  
Ruben Ashotovich Pogosyan ◽  
Olga Vladimirovna Nesterova ◽  
Dmitry Olegovich Bokov ◽  
Irina Alexandrovna Samylina
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Quantitative Determination ◽  
Gallic Acid ◽  
Carboxylic Acids ◽  
High Performance ◽  
Fruit Pulp ◽  
Pomegranate Fruit

Objective: Pomegranate (Punica granatum L.) is a broadly used plant possessing a wide range of medicinal properties. In this research, we have mainly focused on the investigation of phenolic compounds of pomegranate fruit pulp (PFP).Methods: Fresh fruits of “Çəhrayı Gülöyşə,” “Kizil-anor,” and pomegranate varietal mixture were used as samples. High-performance liquid chromatography-ultraviolet (HPLC-UV) analysis of phenol carboxylic acids was performed with metal column Kromasil® C18 (4.6×250 mm, particle size 5 μm) and the acetonitrile-water-concentrated acid phosphoric system (400:600:5) under isocratic elution conditions (flow rate of 0.5 ml/min). Detection was carried out using a UV detector “GILSTON” UV/Visible model 151 at a wavelength of 280 nm.Results and Discussion: As a result of our research, we proposed chromatographic conditions for the separation of phenolic compounds, the conditions for sample preparation of PFP. Procedure for determination of phenolic carboxylic acids total content in terms of gallic acid by HPLC-UV method was developed. According to the obtained data, the content of phenolic carboxylic acids should be at least 0.7%.Conclusion: Procedure for the quantitative determination of gallic acid using the HPLC-UV method was developed. This method which can be used in the standardization of new medicinal plant raw materials - PFP, as well as extract preparations based on it in the future.

scholarly journals Qualitative and quantitative analysis of gallic acid in Alchemilla vulgaris, Allium ursinum, Acorus calamus and Solidago virga-aurea by chip-electrospray ionization mass spectrometry and high performance liquid chromatography

2010 ◽  
Vol 8 (3) ◽  
pp. 530-535 ◽  
Author(s):  
Dumitru Condrat ◽  
Cristina Mosoarca ◽  
Alina Zamfir ◽  
Florin Crişan ◽  
Maria Szabo ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Gallic Acid ◽  
High Performance ◽  
Aerial Part ◽  
Acorus Calamus ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative ◽  
Allium Ursinum

AbstractThis study presents the results obtained from qualitative and quantitative analysis of gallic acid from hydro-alcoholic extracts (methanol, ethanol) of plants from Plantae regnum. Plant qualitative analysis was performed using a novel mass spectrometric (MS) method based on fully automated chip-nanoelectrospray ionization (nanoESI) high capacity ion trap (HCT) while quantitative analysis was carried out by high performance liquid chromatography (HPLC). These methods were applied to Alchemilla vulgaris — common lady’s-mantle (aerial part), Allium ursinum — bear’s garlic (leaves), Acorus calamus — common sweet flag (roots), Solidago virga-aurea — goldenrod (aerial part). Obtained results indicated that methanol extracts (96%, 80%) have a gallic acid content ranging between 0.0011–0.0576 mg mL−1 extract while the ethanol extracts (96%, 60%) exhibit a gallic acid concentration that varies between 0.0010–0.0182 mg mL−1 extract.

scholarly journals Evaluation of a Subset of the Solanum lycopersicum var. cerasiforme Core Collection for Horticultural Quality and Fruit Phenolic Content

HortScience ◽  
2011 ◽  
Vol 46 (11) ◽  
pp. 1450-1455 ◽  
Author(s):  
Peter Boches ◽  
Brooke Peterschmidt ◽  
James R. Myers
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Gallic Acid ◽  
Solanum Lycopersicum ◽  
Core Collection ◽  
High Performance ◽  
Phenolic Content ◽  
Total Phenolics ◽  
Fresh Weight ◽  
Caffeoylquinic Acids

One hundred S. l. var. cerasiforme (Dunal) accessions from the ‘Tanksley’ designated core collection were evaluated for horticultural quality under greenhouse conditions. Fourteen selected accessions were grown under field conditions in a replicated trial to evaluate the fruit for phenolic content. Total fruit phenolics ranged from 44 to 82 mg/100 g gallic acid equivalents (GAE) fresh weight (FW) as measured by Folin-Ciocalteau assay (F-C), and 12 to 108 mg/100 g FW as measured by high-performance liquid chromatography (HPLC). Five accessions (LA1712, LA1455, LA2633, LA1668, and LA2632) had significantly higher total phenolics (F-C) than cultivars (P ≤ 0.05). These five accessions also possessed interesting phenolics profiles, including high levels of caffeoylquinic acids (LA1620, LA1455, and LA2633) and rutin (LA2633).

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