scholarly journals Genomics and Phenotypical Characterization of Two New Lytic Bacteriophages for Biocontrol of Salmonella enterica

2020 ◽  
pp. 93-105
Author(s):  
Emanuele Serro Pottker ◽  
Bruna Webber ◽  
Samuel Cibulski ◽  
Ricardo Zanella ◽  
Viviane Girardi ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Rio Grande Do Sul ◽  
Lytic Action ◽  
Transmission Electron ◽  
The Family ◽  
The University ◽  
Veterinary Hospital ◽  
Salmonella Phage ◽  
Mycology Laboratory

Aims: To perform the isolation, characterization and sequencing of the bacteriophages. To demonstrate that the bacteriophages can be used for biocontrol of different Salmonella enterica serovars. Study Design: This study was an experimental study. Place and Duration of Study: Bacteriology and Mycology Laboratory in the Veterinary Hospital at the Faculty of Agronomy and Veterinary Medicine of the University of Passo Fundo (FAMV/UPF), Biotechnology Center (CBiotec) of the Federal University of Paraíba (UFPB), Center for Microscopy and Microanalysis at the Faculty of Veterinary of the Federal University of Rio Grande do Sul (UFRGS), between January – September 2016. Methodology: Twelve Salmonella enterica serovars (S. Anatum, S. Agona, S. Brandenburg, S. Bredeney, S. Infantis, S. Lexington, S. Panama, S. Rissen, S. Schwarzengrund, S. Tennessee, S. Enteritidis ATCC 13076 and S. Typhimurium ATCC 14028) were selected to be the hosts. We isolate, purify, produce and determine the bacteriophage titers to verify the potential for lysis of these phages against the hosts. Having determined the action of the phages against the hosts, we performed the sequencing of the bacteriophages on the Illumina Mi-Seq platform and the morphology was performed by transmission electron microscopy (TEM). Results: We isolated, characterized and sequenced the genome of two new bacteriophages, Salmonella phage UPF_BP1, belonging to the family Podoviridae and Salmonella phage UPF_BP2, family Myoviridae. UPF_BP1 has lytic action against seven tested Salmonella enterica serovars, while UPF_BP2 has action against the twelve tested serovars. Conclusion: The two new bacteriophages have a lytic action against different Salmonella enterica serovars, feeding our expectations for the development of alternatives for the use of antimicrobials, being possible candidates for use as a biocontrol of Salmonella enterica in food, animals and the environment.

scholarly journals Synthesis and superstructural characterization of Fe1.89Mo4.11O7

1994 ◽  
Vol 9 (4) ◽  
pp. 891-897 ◽  
Author(s):  
George L. Schimek ◽  
Robert E. McCarley ◽  
L. Scott Chumbley
Keyword(s):  
Structure Type ◽  
Tetrahedral Coordination ◽  
X Ray Diffraction ◽  
Cation Site ◽  
X Ray ◽  
Transmission Electron ◽  
The Family ◽  
Regular Arrangement ◽  
Cation Sites

Superstructuring in the new compound Fe1.89Mo4.11O7 has been elucidated by transmission electron microscopy. This compound is a member of the family M2MO4O7 and has both iron and molybdenum atoms occupying octahedrally coordinated sites in the structure, represented by Fet(Fe0.89M0.11)0Mo4O7. The superstructuring, detected only by electron diffraction, involved tripling of all three lattice parameters of the subcell. The subcell was structured by single crystal x-ray diffraction [Imma, no. 74, a = 5.9793(5) Å, b = 5.7704(4) Å, and c = 17.036(1) Å]. This structure type contains a close-packed arrangement of Mo4O7 units, which are infinite chains of trans edge-shared molybdenum octahedra running parallel to b*. Two different coordination environments are observed for the cations. Parallel to the a* direction, infinite edge-sharing MO6 (M = 89% Fe or 11% Mo) octahedra are observed. The second cation site, with nearly tetrahedral coordination by oxygen, is filled solely by iron. The superstructure can be rationalized by a regular arrangement of iron and molybdenum atoms in the octahedrally coordinated cation sites.

scholarly journals Aquatic macrophytes of six subtropical shallow lakes, Rio Grande, Rio Grande do Sul, Brazil

Check List ◽  
2012 ◽  
Vol 8 (2) ◽  
pp. 187 ◽  
Author(s):  
Sabrina Amaral Pereira ◽  
Cláudio Rossano Trindade Trindade ◽  
Edélti Faria Albertoni ◽  
Cleber Palma-Silva
Keyword(s):  
Shallow Lakes ◽  
Aquatic Macrophytes ◽  
Rio Grande ◽  
Rio Grande Do Sul ◽  
The South ◽  
The Family ◽  
South Of Brazil ◽  
The University

The aim of this study was to document the richness of aquatic macrophytes in six shallow lakes at Federal University of Rio Grande - FURG, Rio Grande, Rio Grande do Sul, Brazil. Qualitative surveys were carried out during 2008. Specimens were deposited at the university herbarium (HURG). We recorded 44 species belonging to 35 genera and 21 families. The majority of species were present all year. The family with highest diversity was Cyperaceae (eight species). Other studies have reported approximately 170 species for wetlands in the south of Brazil. This study documented approximately 25% of these species. The flora of the area should be preserved and monitored. 

scholarly journals Characterization of a Novel Mitovirus Infecting Melanconiella theae Isolated From Tea Plants

2021 ◽  
Vol 12 ◽  
Author(s):  
Karim Shafik ◽  
Muhammad Umer ◽  
Huafeng You ◽  
Hamdy Aboushedida ◽  
Zhenhua Wang ◽  
...  
Keyword(s):  
Genomic Sequence ◽  
Dsrna Segment ◽  
Rna Dependent Rna Polymerase ◽  
Stem Loop ◽  
Transmission Electron ◽  
The Family ◽  
Dsrna Genome ◽  
Tea Plants ◽  
Rich Content

A dsRNA segment was identified in the fungus Melanconiella theae isolated from tea plants. The complete dsRNA sequence, determined by random cloning together with RACE protocol, is 2,461 bp in length with an AU-rich content (62.37%) and comprises a single ORF of 2,265-nucleotides encoding an RNA-dependent RNA-polymerase (RdRp, 754 amino acids in size). The terminus sequences can fold into predicted stable stem-loop structures. A BLASTX and phylogenetic analysis revealed the dsRNA genome shows similarities with the RdRp sequences of mitoviruses, with the highest identity of 48% with those of grapevine-associated mitovirus 20 and Colletotrichum fructicola mitovirus 1. Our results reveal a novel member, tentatively named Melanconiella theae mitovirus 1 (MtMV1), belongs to the family Mitoviridae. MtMV1 is capsidless as examined by transmission electron microscope, efficiently transmitted through conidia as 100 conidium-generated colonies were analyzed, and easily eliminated by hyphal tipping method combined with green-leaf tea powder. MtMV1 has a genomic sequence obviously divergent from those of most members in the family Mitoviridae and some unique characteristics unreported in known members. This is the first report of a mycovirus infecting Melanconiella fungi to date.

Phenotypic and genetic characterization of Salmonella enterica subsp. enterica serovar Typhimurium isolated from pigs in Rio Grande do Sul, Brazil

2007 ◽  
Vol 83 (3) ◽  
pp. 302-310 ◽  
Author(s):  
Marjo Cado Bessa ◽  
Geovana Brenner Michael ◽  
Nunzia Canu ◽  
Cláudio Wageck Canal ◽  
Marisa Cardoso ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Genetic Characterization ◽  
Rio Grande ◽  
Rio Grande Do Sul ◽  
Serovar Typhimurium ◽  
Phenotypic And Genetic Characterization

scholarly journals Electron Microscopy in Discovery of Novel and Emerging Viruses from the Collection of the World Reference Center for Emerging Viruses and Arboviruses (WRCEVA)

Viruses ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 477 ◽  
Author(s):  
Vsevolod L. Popov ◽  
Robert B. Tesh ◽  
Scott C. Weaver ◽  
Nikos Vasilakis
Keyword(s):  
Electron Microscopy ◽  
Emerging Viruses ◽  
Intracellular Location ◽  
Reference Center ◽  
Cytopathic Effects ◽  
Transmission Electron ◽  
The Family ◽  
The World ◽  
Identification And Characterization

Since the beginning of modern virology in the 1950s, transmission electron microscopy (TEM) has been an important and widely used technique for discovery, identification and characterization of new viruses. Using TEM, viruses can be differentiated by their ultrastructure: shape, size, intracellular location and for some viruses, by the ultrastructural cytopathic effects and/or specific structures forming in the host cell during their replication. Ultrastructural characteristics are usually sufficient for the identification of a virus to the family level. In this review, we summarize 25 years of experience in identification of novel viruses from the collection of the World Reference Center for Emerging Viruses and Arboviruses (WRCEVA).

scholarly journals Sir Robert William Kerr Honeycombe KBE. 2 May 1921—14 September 2007

2009 ◽  
Vol 55 ◽  
pp. 91-106 ◽  
Author(s):  
R. E. Smallman
Keyword(s):  
State Of The Art ◽  
Microstructural Characterization ◽  
University Education ◽  
Research Experience ◽  
Interphase Precipitation ◽  
Transmission Electron ◽  
Alloy Steels ◽  
The Uk ◽  
The University

Although Robert Honeycombe was born in Melbourne, Australia, where he received his university education and gained valuable research experience at the Council for Scientific and Industrial Research, it was in the UK that his distinguished career developed. At Sheffield he harnessed the newly emerging technique of transmission electron microscopy to the microstructural study of alloy steels. Moving to Cambridge, he built up a world-renowned team in this area. His seminal work was the characterization of interphase precipitation at the α/γboundary interface, which had a perceptible impact on the production of micro-alloyed steels throughout the world. As Goldsmiths' Professor of Metallurgy he was the longest-serving head of the department, broadening its coverage into material science to include ceramics and polymers as well as reinforcing the traditional areas of mechanical behaviour and processing. All these activities were underpinned by state-of-the-art microstructural characterization. Robert had an open and warm personality and was a natural leader of the profession within the university, in learned societies and in government and research council bodies. Above all he was a great supporter of young researchers, not only at Cambridge but also throughout the country.

Characterization of the Carbon-Coating of LiFePO4 by Transmission Electron Microscopy and Raman Spectroscopy

2006 ◽  
Vol 972 ◽  
Author(s):  
Michel Massot ◽  
Karim Zaghib ◽  
Alain Mauger ◽  
François Gendron ◽  
Christian M Julien
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Discharge Rate ◽  
Electronic Conductivity ◽  
Carbon Layer ◽  
Raman Scattering Spectroscopy ◽  
Transmission Electron ◽  
High Electronic Conductivity ◽  
The Family

AbstractWe present the properties of the carbon layer deposited at the surface of the LiFePO4 particles. Characterizations include scanning electron microscopy, high-resolution transmission electron microscopy, and Raman scattering spectroscopy. Analuysis of Raman spectra reveals that the carbon deposit is hydrogenated with very small hydrogen/carbon ratio, so that it belongs to the family of the amorphous graphitic carbon. It is expected to have the same properties (small hardness, high electronic conductivity) that favor both the Li diffusion from the LiFePO4 bulk and the charge-discharge rate of the cell.

scholarly journals Molecular Characterization of a Novel Wheat-infecting Virus in Family Betaflexiviridae

2021 ◽  
Author(s):  
Jianxiang Wu ◽  
Shuai Fu ◽  
Mengzhu He ◽  
Bingjian Sun ◽  
Xueping Zhou
Keyword(s):  
Triple Gene Block ◽  
International Committee ◽  
Open Reading Frames ◽  
Yellow Mosaic Virus ◽  
Wheat Yellow Mosaic Virus ◽  
Gene Block ◽  
Transmission Electron ◽  
The Family ◽  
Novel Virus

Abstract Wheat plants showing yellow mosaic in leaves and stunting were observed and collected from wheat fields in the Henan Province, China. Analyses of these plants through transmission electron microscopy showed that these plants contained two filamentous virus-like particles of 200–500 nm and 1000–1300 nm long, respectively. RNA-seq result unveiled a co-infection of wheat yellow mosaic virus (WYMV) and an unknown wheat-infecting virus. The complete genome sequence of the unknown virus is 8,410 nucleotide long, excluding its 3’ end poly (A) tail. This unknown virus has six open reading frames (ORFs). The ORF1 encodes a putative viral replication-associated protein, and the ORF2, 3 and 4 encode the triple gene block (TGB) proteins. The ORF5 and 6 encode the capsid protein (CP) and a protein with unknown function, respectively. Phylogenetic relationship analyses showed that this novel virus is evolutionarily related to viruses in the subfamily Quinvirinae, family Betaflexiviridae. It is, however, distinctly different from the viruses in other genera. Based on the species and genus demarcation criteria set by the International Committee on Taxonomy of Viruses (ICTV), we tentatively name this novel virus as wheat yellow stunt-associated betaflexivirus (WYSaBV). We also propose WYSaBV as a new member in a new genus in the family Betaflexiviridae.

The Use of Advanced Analytical Techniques for Characterization of the Chemical, Physical, and Crystallographic Nature of a Surface

1973 ◽  
Vol 31 ◽  
pp. 132-133 ◽  
Author(s):  
R. E. Herfert
Keyword(s):  
Surface Characterization ◽  
Analytical Techniques ◽  
X Ray Diffraction ◽  
Depth Of Penetration ◽  
X Ray ◽  
The Past ◽  
Transmission Electron ◽  
Scanning Electron

Studies of the nature of a surface, either metallic or nonmetallic, in the past, have been limited to the instrumentation available for these measurements. In the past, optical microscopy, replica transmission electron microscopy, electron or X-ray diffraction and optical or X-ray spectroscopy have provided the means of surface characterization. Actually, some of these techniques are not purely surface; the depth of penetration may be a few thousands of an inch. Within the last five years, instrumentation has been made available which now makes it practical for use to study the outer few 100A of layers and characterize it completely from a chemical, physical, and crystallographic standpoint. The scanning electron microscope (SEM) provides a means of viewing the surface of a material in situ to magnifications as high as 250,000X.

An Electron Microscope Study of Interdiffusion and Compound Formation in Ta-Au Thin Films

1973 ◽  
Vol 31 ◽  
pp. 32-33 ◽  
Author(s):  
T. C. Tisone ◽  
S. Lau
Keyword(s):  
Electron Microscope ◽  
Electron Microscope Study ◽  
Thermally Grown Oxide ◽  
Ion Milling ◽  
Compound Formation ◽  
Technology Application ◽  
Transmission Electron ◽  
Before And After ◽  
Au Thin Films

In a study of the properties of a Ta-Au metallization system for thin film technology application, the interdiffusion between Ta(bcc)-Au, βTa-Au and Ta2M-Au films was studied. Considered here is a discussion of the use of the transmission electron microscope(TEM) in the identification of phases formed and characterization of the film microstructures before and after annealing.The films were deposited by sputtering onto silicon wafers with 5000 Å of thermally grown oxide. The film thicknesses were 2000 Å of Ta and 2000 Å of Au. Samples for TEM observation were prepared by ultrasonically cutting 3mm disks from the wafers. The disks were first chemically etched from the silicon side using a HNO3 :HF(19:5) solution followed by ion milling to perforation of the Au side.

Sign in / Sign up

Export Citation Format

Share Document