This paper presents an uncertain logistic growth model to analyse and predict the evolution of the cumulative number of COVID-19 infection in Czech Republic. Some fundamental knowledge about the uncertain regression analysis are reviewed firstly. Stochastic regression analysis is invalid to model cumulative number of confirmed COVID-19 cases in Czech Republic, by considering the disturbance term as random variables, because that the normality test and the identical distribution test of residuals are not passed, and the residual plot does not look like a null plot in the sense of probability theory. In this case, the uncertain logistic growth model is applied by characterizing the disturbance term as uncertain variables. Then parameter estimation, residual analysis, the forecast value and confidence interval are studied. Additionally, the uncertain hypothesis test is proposed to evaluate the appropriateness of the fitted logistic growth model and estimated disturbance term. The analysis and prediction for the cumulative number of COVID-19 infection in Czech Republic can propose theoretical support for the disease control and prevention. Due to the symmetry and similarity of epidemic transmission, other regions of COVID-19 infections, or other diseases can be disposed in a similar theory and method.