Detection and Molecular Characterization of a Novel Species of Circovirus in a Tawny Owl (Strix aluco) in Southern Italy

Thanks to recent developments in molecular methods, many new species have been discovered within the genus Circovirus, which comprises viruses of veterinary relevance found in a broad range of hosts. In particular, several circoviruses are known to infect birds, often causing immunosuppression and feathering disorders. Nonetheless, nothing is known about their circulation in birds of prey. In this study, samples from 61 birds of prey representing ten different species, recovered by a wildlife rescue centre in Southern Italy, were taken at necropsy and analysed by PCR with pan-Circovirus primers. Only one sample, collected from a tawny owl (Strix aluco), tested positive. Its genome, sequenced by primer walking, displays the typical features of circoviruses. Based on demarcation criteria, the detected strain qualifies as a novel species, which was named “tawny owl-associated circovirus” (ToCV). Phylogenetically, ToCV clustered with mammalian rather than avian circoviruses, and its closeness to a rodent circovirus suggests that its host may have been a micromammal eaten by the tawny owl. On the other hand, its detection in the spleen fits with the tropism of other avian circoviruses. Little can be therefore said on its biology and pathogenicity, and further efforts are needed to better characterize its epidemiology.

The Problem of Demarcation of Information Conflicts: Philosophical-Methodological Analysis

The phenomenon of information conflict at the moment is an actual research object of many social and humanitarian disciplines. On the other hand, there is a lack of fundamental theoretical, primarily philosophical and methodological, research on this issue. This is expressed, inter alia, in the absence of philosophical and methodological grounds for isolating an information conflict from the totality of all objectively observed communications, i.e. demarcation of the phenomenon. The problem of finding criteria for an information conflict is of key importance in the formation of the methodological foundations and categorical apparatus for studying this phenomenon. In this regard, the need to develop theoretical foundations for the demarcation of information conflict is an urgent research task. The object of this work is the phenomenon of information conflict as part of the communicative process. The subject of the research is the problem of demarcation of information conflicts from all aggregate communications. The purpose of the study is to describe and substantiate the existing object-oriented methodologies that allow for the demarcation procedure. The following tasks are adequately formulated for the purpose of the study: 1) describe the problem of demarcation; 2) identify the communicative grounds of the information conflict; 3) analyze and highlight the main approaches to the problem of demarcation of information conflict. The novelty of the work lies in the systematic description and structuring of methodological approaches to the problem of demarcation of information conflicts, as well as analysis of the boundaries of methodologies and approaches. In the course of the work, it was determined and proved that, relying solely on the theory of communication, it is impossible to adequately carry out the demarcation procedure; it is necessary to use additional, non-communicative demarcation criteria, which is due to the variety of demarcation approaches in various studies. In conclusion, conclusions are formulated about the limitations of object-oriented approaches for solving the problem of demarcation of information conflicts.

A Novel Subtype of Bovine Hepacivirus Identified in Ticks Reveals the Genetic Diversity and Evolution of Bovine Hepacivirus

Hepaciviruses represent a group of viruses that pose a significant threat to the health of humans and animals. New members of the genus Hepacivirus in the family Flaviviridae have recently been identified in a wide variety of host species worldwide. Similar to the Hepatitis C virus (HCV), bovine hepacivirus (BovHepV) is hepatotropic and causes acute or persistent infections in cattle. BovHepVs are distributed worldwide and classified into two genotypes with seven subtypes in genotype 1. In this study, three BovHepV strains were identified in the samples of ticks sucking blood on cattle in the Guangdong province of China, through unbiased high-throughput sequencing. Genetic analysis revealed the polyprotein-coding gene of these viral sequences herein shared 67.7–84.8% nt identity and 76.1–95.6% aa identity with other BovHepVs identified worldwide. As per the demarcation criteria adopted for the genotyping and subtyping of HCV, these three BovHepV strains belonged to a novel subtype within the genotype 1. Additionally, purifying selection was the dominant evolutionary pressure acting on the genomes of BovHepV, and genetic recombination was not common among BovHepVs. These results expand the knowledge about the genetic diversity and evolution of BovHepV distributed globally, and also indicate genetically divergent BovHepV strains were co-circulating in cattle populations in China.

Complete genome sequence of a novel fish papillomavirus detected in farmed wels catfish (Silurus glanis)

A novel papillomavirus (PV) was detected in farmed wels catfish (Silurus glanis) in Hungary showing clinical signs resembling those of wels catfish herpesvirus disease. The whole genome of Silurus glanis papillomavirus 1 (SgPV1) was identified using next-generation sequencing. The 5,612-bp complete genome contains four predicted protein coding regions (E1, E2, L1, and L2), which seem to have homologues in every PV genome sequenced to date. Five complete fish PV genome sequences are available in the GenBank database. Their genomes range between 5,748 and 6,086 bp and contain the minimal PV backbone genes E1, E2, L2, and L1, unlike PVs of higher vertebrates, which have larger genomes (6.8-8.6 kbp) and additional (onco)genes. Considering the current species demarcation criteria for the family Papillomaviridae, the establishment of a novel species named "Nunpapillomavirus siluri" is proposed for the SgPV1 in a novel genus, "Nunpapillomavirus", in the subfamily Secondpapillomavirinae.

Lagos Bat Virus, an Under-Reported Rabies-Related Lyssavirus

Lagos bat virus (LBV), one of the 17 accepted viral species of the Lyssavirus genus, was the first rabies-related virus described in 1956. This virus is endemic to the African continent and is rarely encountered. There are currently four lineages, although the observed genetic diversity exceeds existing lyssavirus species demarcation criteria. Several exposures to rabid bats infected with LBV have been reported; however, no known human cases have been reported to date. This review provides the history of LBV and summarizes previous knowledge as well as new detections. Genetic diversity, pathogenesis and prevention are re-evaluated and discussed.

Metagenomic Analyses of Nepoviruses: Diversity, Evolution and Identification of a Hitherto Undescribed Putative Region for Host Range in Subgroup a Species

Datamining and metagenomic analyses of 277 open reading frame sequences of bipartite RNA viruses and variants in the genus Nepovirus documented how delicate it can be to unequivocally identify species, in particular subgroup A and C species, based on some of the currently adopted taxonomic demarcation criteria. It suggests a possible need for their amendment to accommodate pangenome information. In addition, we revealed a host-dependent structure of arabis mosaic virus (ArMV) populations at a cladistic level and confirmed a phylogeographic structure of grapevine fanleaf virus (GFLV) populations. We also identified new putative recombinant events for species of subgroups A, B and C. The evolutionary specificity of some capsid regions of ArMV and GFLV that were previously described and biologically validated as vector determinant was circumscribed in silico. Furthermore, a C-terminal segment of the RNA-dependent RNA polymerase of subgroup A species was predicted as a putative host range determinant based on statistically supported higher π values for GFLV and ArMV isolates infecting Vitis spp. compared to non-Vitis infecting ArMV isolates. This study illustrated how sequence information obtained via high throughput sequencing can increase our understanding of mechanisms that modulate virus diversity and evolution and create new opportunities for advancing studies on the biology of economically important plant viruses.

Genetic and Antigenetic Characterization of the Novel Kotalahti Bat Lyssavirus (KBLV)

There is a growing diversity of bat-associated lyssaviruses in the Old World. In August 2017, a dead Brandt’s bat (Myotis brandtii) tested positive for rabies and based on partial sequence analysis, the novel Kotalahti bat lyssavirus (KBLV) was identified. Because the bat was in an autolyzed state, isolation of KBLV was neither successful after three consecutive cell passages on cells nor in mice. Next generation sequencing (NGS) was applied using Ion Torrent ™ S5 technology coupled with target enrichment via hybridization-based capture (myBaits®) was used to sequence 99% of the genome, comprising of 11,878 nucleotides (nt). KBLV is most closely related to EBLV-2 (78.7% identity), followed by KHUV (79.0%) and BBLV (77.6%), supporting the assignment as phylogroup I lyssavirus. Interestingly, all of these lyssaviruses were also isolated from bat species of the genus Myotis, thus supporting that M. brandtii is likely the reservoir host. All information on antigenic and genetic divergence fulfil the species demarcation criteria by ICTV, so that we recommend KBLV as a novel species within the Lyssavirus genus. Next to sequence analyses, assignment to phylogroup I was functionally corroborated by cross-neutralization of G-deleted RABV, pseudotyped with KBLV-G by sera from RABV vaccinated humans. This suggests that conventional RABV vaccines also confer protection against the novel KBLV.

Genetic Diversity of Potyviruses Associated with Tulip Breaking Syndrome

Tulip breaking is economically the most important viral disease of modern-day tulip growing. It is characterized by irregular flame and feather-like patterns in the flowers and mosaic on the foliage. Thirty-two leaf samples were collected from cultivated tulip plants showing tulip breaking syndrome from Hungary in 2017 and 2018. Virus identification was performed by serological (ELISA) and molecular (RT-PCR) methods. All samples proved to be infected with a potyvirus and evidence was provided that three potyvirus species could be identified in the samples: Lily mottle virus (LMoV), Tulip breaking virus (TBV) and Rembrandt tulip-breaking virus (ReTBV). Recombination prediction accomplished with Recombination Detection Program (RDP) v4.98 revealed potential intraspecies recombination in the case of TBV and LMoV. Phylogenetic analyses of the coat protein (CP) regions proved the monophyletic origin of these viruses and verified them as three different species according to current International Committee on Taxonomy of Viruses (ICTV) species demarcation criteria. Based on these results, we analyzed taxonomic relations concerning potyviruses associated with tulip breaking syndrome. We propose the elevation of ReTBV to species level, and emergence of two new subgroups in ReTBV.

Does Nietzsche have a “Nachlass”?

Based on a review of the literature and historical evidence, I argue that the use of the methodological principle known as the priority principle in Anglo-American Nietzsche scholarship is inconsistent and irreconcilable with historical evidence. It attempts to demarcate between the published works and the Nachlass. However, there are no agreed upon necessary and sufficient conditions of a particular textual object being considered “Nachlass.” This absence leads to implicit and often tacit value demarcation criteria that can be broadly grouped into four types of consideration: publication, authorization, publicness, and audience. Each of these criteria pick out a different set of texts as “Nachlass.” Thus, despite the veneer of agreement, the most broadly accepted methodological approach in the Anglo-American tradition of Nietzsche scholarship is applied inconsistently. I argue that we must either offer necessary and sufficient conditions for a piece of text being Nachlass, or we ought to abandon such abstract criteria altogether and embrace a contextual and historical approach. I then argue that the first option is impossible given historical evidence. I conclude this article by explicating several recent German approaches to the Nachlass which I think can offer a new possible approach.