Genome of the parasitoid wasp Dinocampus coccinellae reveals extensive duplications, accelerated evolution, and independent origins of thelytokous parthenogeny and solitary behavior

Dinocampus coccinellae (Hymenoptera: Braconidae) is a generalist parasitoid wasp that parasitizes >50 species of predatory lady beetles (Coleoptera: Coccinellidae), with thelytokous parthenogeny as its primary mode of reproduction. Here we present the first high quality genome of D. coccinellae using a combination of short and long read sequencing technologies, followed by assembly and scaffolding of chromosomal segments using Chicago+HiC technologies. We also present a first-pass ab initio and a reference-based genome annotation, and resolve timings of divergence and evolution of (1) solitary behavior vs eusociality, (2) arrhenotokous vs thelytokous parthenogenesis, and (3) rates of gene loss and gain among Hymenopteran lineages. Our study finds (1) at least two independent origins of eusociality and solitary behavior among Hymenoptera, (2) two independent origins of thelytokous parthenogenesis from ancestral arrhenotoky, and (3) accelerated rates of gene duplications, loss, and gain along the lineages leading to D. coccinellae. Our work both affirms the ancient divergence of Braconid wasps from ancestral Hymenopterans and accelerated rates of evolution in response to adaptations to novel hosts, including polyDNA viral co-evolution.

Early Adolescents’ Social Achievement Goals and Perceived Relational Support: Their Additive and Interactive Effects on Social Behavior

The current study examined the additive and interactive effects of early adolescents’ social achievement goals and perceived relational support from teachers and peers on their social behavior. Adolescents’ social achievement goals (i.e., social development, social demonstration-approach, and social demonstration-avoidance), perceived relational support from teachers and peers, and social behavior (i.e., overt and relational aggression, prosocial behavior, and anxious solitary behavior) were assessed in a sample of fifth and sixth graders (Mage = 12.5; N = 677) nested within 26 classrooms. Multilevel modeling results indicated that social goals and relational support from teachers and peers made additive contributions to adolescents’ social behavior. Results also indicated the evidence of interactive effects, such that relational support from teachers was negatively associated with overt and relational aggression primarily among adolescents who had high social demonstration-approach goals. Findings underscore the need to consider adolescents’ social goals in conjunction with their perceived relational support for educators and practitioners.

Artificial Termite-Fishing Tasks as Enrichment for Sanctuary-Housed Chimpanzees: Behavioral Effects and Impact on Welfare

Artificial termite-fishing tasks are a common enrichment for captive great apes, promoting species-typical behaviors. Nonetheless, whether these activities are linked to changes in other behaviors and whether these changes persist over time has seldom been investigated. We assessed whether the use of an artificial termite-fishing task was linked to changes in the solitary behavior and social dynamics in two groups of sanctuary-housed chimpanzees (Pan troglodytes). Specifically, we compared chimpanzee behavior during eight enrichment sessions distributed over a two-month period, with similar periods before and after the introduction of the enrichment. Data were collected from combined interval and continuous sampling methods and were analyzed using generalized linear mixed models. We found that participation increased across sessions and that both enrichment and participation predicted an increase in tool use and feeding and a decrease in inactivity, which were all maintained throughout the sessions. Furthermore, participation was positively associated with social proximity, revealing a gathering effect of the task. However, neither enrichment nor participation were linked to changes in abnormal, self-directed, affiliation-related or aggression-related behaviors. Overall, our results support the hypothesis that artificial termite-fishing is a suitable enrichment for captive chimpanzees, maintaining the subjects’ interest and promoting species-typical behaviors, with no negative effects on social activities.

Jaguar Firms: Tropic Dwellers, Camouflage Masters, and Solitary Predators

ABSTRACT Within the last two decades, the international expansion of Latin American companies has undergone remarkable growth. This phenomenon has attracted scholarly attention, however, most of the available research is focused on companies that have already engaged in foreign direct investment (FDI), meanwhile, Latin American firms in pre-FDI stages remain mostly understudied. This article uses an explanatory case study design to analyze the corporate reputation and decision-making process related to international expansion of a set of ten Latin American companies. Both archival and primary data were used in the individual and cross-case analyses stages for 22 months. Our study identifies and establishes analytical generalizations when examining and contrasting the findings with the previously revised theoretical frameworks. More specifically, we identified that these companies exhibit similarities with the behavior of Jaguars, the Latin American wild feline; especially because of (i) their preference to remain in their regional market to exploit current capabilities and advantages, and eventually enter developed markets to upgrade capabilities and surpass strong competitors at home; (ii) their strategies to disguise their country of origin and lack of experience when operating internationally; and (iii) their solitary behavior and reluctance to engage in partnerships and/or strategic alliances unless they have a specific interest in building legitimacy and enhancing reputation.

Genome of the parasitoid wasp Dinocampus coccinellae reveals extensive duplications, accelerated evolution, and independent origins of thelytokous parthenogeny and solitary behavior

Dinocampus coccinellae (Hymenoptera: Braconidae) is a generalist parasitoid wasp that parasitizes >50 species of predatory lady beetles (Coleoptera: Coccinellidae), with thelytokous parthenogeny as its primary mode of reproduction. Here we present the first high quality genome of D. coccinellae using a combination of short and long read sequencing technologies, followed by assembly and scaffolding of chromosomal segments using Chicago+HiC technologies. We also present a first-pass ab initio genome annotation, and resolve timings of divergence and evolution of (1) solitary behavior vs eusociality, (2) arrhenotokous vs thelytokous parthenogenesis, and (3) rates of gene loss and gain among Hymenopteran lineages. Our study finds (1) at least two independent origins of eusociality and solitary behavior among Hymenoptera, (2) two independent origins of thelytokous parthenogenesis from ancestral arrhenotoky, and (3) accelerated rates of gene duplications, loss, and gain along the lineages leading to D. coccinellae. Our work both affirms the ancient divergence of Braconid wasps from ancestral Hymenopterans and accelerated rates of evolution in response to adaptations to novel hosts, including polyDNA viral co-evolution.

Search Behavior of Individual Foragers Involves Neurotransmitter Systems Characteristic for Social Scouting

In honey bees search behavior occurs as social and solitary behavior. In the context of foraging, searching for food sources is performed by behavioral specialized foragers, the scouts. When the scouts have found a new food source, they recruit other foragers (recruits). These recruits never search for a new food source on their own. However, when the food source is experimentally removed, they start searching for that food source. Our study provides a detailed description of this solitary search behavior and the variation of this behavior among individual foragers. Furthermore, mass spectrometric measurement showed that the initiation and performance of this solitary search behavior is associated with changes in glutamate, GABA, histamine, aspartate, and the catecholaminergic system in the optic lobes and central brain area. These findings strikingly correspond with the results of an earlier study that showed that scouts and recruits differ in the expression of glutamate and GABA receptors. Together, the results of both studies provide first clear support for the hypothesis that behavioral specialization in honey bees is based on adjusting modulatory systems involved in solitary behavior to increase the probability or frequency of that behavior.

Extensive aquatic subsidies lead to territorial breakdown and high density of an apex predator

Energetic subsidies between terrestrial and aquatic ecosystems can strongly influence food webs and population dynamics. Our objective was to study how aquatic subsidies affected jaguar (Panthera onca) diet, sociality, and population density in a seasonally flooded protected area in the Brazilian Pantanal. The diet (n = 138 scats) was dominated by fish (46%) and aquatic reptiles (55%), representing the first jaguar population known to feed extensively on fish and to minimally consume mammals (11%). These aquatic subsidies supported the highest jaguar population density estimate to date (12.4 per 100 km2) derived from camera traps (8,065 trap nights) and GPS collars (n = 13). Contrary to their mostly solitary behavior elsewhere, we documented social interactions previously unobserved between same-sex adults including cooperative fishing, co-traveling, and play. Our research demonstrates that aquatic subsidies seen in omnivores can be highly influential to obligate carnivores leading to high population density and altered social structure.

Massive loss of transcription factors and the initial diversification of placental mammals

As one of the most successful categories of organisms, mammals occupy a variety of niches on earth as a result of macroevolution. Transcription factors (TFs), the basic regulators of gene expression, may also evolve during mammalian phenotypic diversification and macroevolution. To examine the relationship between TFs and mammalian macroevolution, we analyzed 140,821 de novo-identified TFs and their birth and death histories from 96 mammalian species. Gene tree vs. species tree reconciliation revealed that mammals experienced an upsurge in TF losses around 100 million years ago and also near the K–Pg boundary, thus implying a relationship with the divergence of placental animals. From approximately 100 million years ago to the present, losses dominated TF events without a significant change in TF gains. To quantify the effects of this TF pruning on mammalian macroevolution, we analyzed rates of molecular evolution and expression profiles of regulated target genes. Surprisingly, TF loss decelerated, rather than accelerated, molecular evolutionary rates of their target genes, suggesting increased functional constraints. Furthermore, an association study revealed that massive TF losses are significantly positively correlated with solitary behavior, nocturnality, reproductive-seasonality and insectivory life history traits, possibly through rewiring of regulatory networks.

Search behavior of individual foragers involves neurotransmitter systems characteristic for social scouting

SUMMARYBehavioral specialization in honey bees is regulated by hormones and neuromodulators that tune neuronal activity and gene expression, and can be viewed as a temporarily fixed behavioral state associated with a specific brain state. Honey bee scouts, which search for new food sources, show a higher expression of genes involved in glutamate, GABA and catecholamine signaling than recruits that remain loyal to a food source. We asked whether recruits visiting a feeder initiated a search behavior when the feeder was experimentally removed, and if similar neuromodulators might be involved in the initiation and performance of that behavior. We found that recruits perform a relatively stereotyped search behavior that shows inter-individual variation in its intensity. Quantitative single brain mass spectrometric analyses showed that glutamate and GABA titers changed during search behavior supporting the hypothesis that behavioral specialization in social insects is based on reinforcing brain molecular processes involved in solitary behavior.

Behavior Of Individual Orangutan Sumatera (Pongo Abelii) Before Direintroduction At The Orangutan Quarantine Station, Sibolangit, Deli Serdang, North Sumatera

The individual behavior of orangutan (Pongoabeli) before reintroduction to the wild was studied from March to June 2012 in orangutan quarantine station Batumbelin Sibolangit, Deli Serdang. The research used focal animal sampling method, at the same time the data recording was taken using instantenous method. The result showed that solitary behavior (39.34%) which was dominated by feeding (20.60%). The second highest (35.31%) was resting which was dominating by sitting (16.87%) followed by moving behavior (13.58%) which mostly was branching (7.54%). The least activity was competitive behavior (1.88%) which was dominated by streaking down each other (0.82%). The result also indicated an abnormal behavior among 3 orangutans observed such as eating their own feces.