A low-cost simple test for weekly detection of Mycoplasma hyorhinis and arginini contaminations in cell cultures and viral preparations

2022 ◽  
Vol 299 ◽  
pp. 114327
Author(s):  
Michele Lai ◽  
Elena Iacono ◽  
Pietro Giorgio Spezia ◽  
Giulia Lottini ◽  
Veronica La Rocca ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Low Cost ◽  
Simple Test ◽  
Mycoplasma Hyorhinis

Ratiometric Nanoviscometers: Applications for Measuring Cellular Physical Properties in 3D Cultures

2020 ◽  
Vol 25 (3) ◽  
pp. 234-246
Author(s):  
Charles McRae White ◽  
Mark A. Haidekker ◽  
William S. Kisaalita
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
Cell Biology ◽  
Low Cost ◽  
3D Cell Culture ◽  
Biomechanical Properties ◽  
Practical Applications ◽  
3D Cell Cultures

New insights into the biomechanical properties of cells are revealing the importance of these properties and how they relate to underlying molecular, architectural, and behavioral changes associated with cell state and disease processes. However, the current understanding of how these in vitro biomechanical properties are associated with in vivo processes has been developed based on the traditional monolayer (two-dimensional [2D]) cell culture, which traditionally has not translated well to the three-dimensional (3D) cell culture and in vivo function. Many gold standard methods and tools used to observe the biomechanical properties of 2D cell cultures cannot be used with 3D cell cultures. Fluorescent molecules can respond to external factors almost instantaneously and require relatively low-cost instrumentation. In this review, we provide the background on fluorescent molecular rotors, which are attractive tools due to the relationship of their emission quantum yield with environmental microviscosity. We make the case for their use in both 2D and 3D cell cultures and speculate on their fundamental and practical applications in cell biology.

scholarly journals Predict of Asphalt Rutting Potential Based on IDT and Validation with ANN

2019 ◽  
Vol 9 (2) ◽  
pp. 131-138
Author(s):  
Hassan Divandari
Keyword(s):  
Low Cost ◽  
Permanent Deformation ◽  
Design Procedure ◽  
Mix Design ◽  
Test Results ◽  
Simple Test ◽  
Short Term ◽  
Time Saving ◽  
Simple Method ◽  
Main Method

Abstract Rutting occurs due to accumulation of incrementally small permanent deformations from each load application and it can cause irreparable problems in pavements. On the other hand, the Marshall Mix design which is known as the main method in Iran, the lack of a simple test to determine specimen resistance to permanent deformation as the major reason for asphalt rutting is noticeable. Although today many devices are used for rutting measurement, none of them have the ability to use in wider field. In addition, prevalent methods of evaluating rutting potential are usually costly and time consuming. Mentioned parameters illustrate the necessity of developing a simple method, not only having fine precision, but also are able to predict rutting performance with low cost in the short term in laboratory. In this research, after performing the main tests on specimens, IDT test results and Marshall Parameters were used to develop a mathematical model to estimate specimen rut depth. The model is validated by using ANN and makes it possible to evaluate mixtures rutting potential while OBC is being determined in laboratory. So not only is there no need to use expensive instruments of rutting test, but also a remarkable time saving in mix design procedure is achievable.

Inkjet-printed PEDOT:PSS multi-electrode arrays for low-cost in vitro electrophysiology

Lab on a Chip ◽  
2019 ◽  
Vol 19 (22) ◽  
pp. 3776-3786 ◽  
Author(s):  
Leonardo D. Garma ◽  
Laura M. Ferrari ◽  
Paola Scognamiglio ◽  
Francesco Greco ◽  
Francesca Santoro
Keyword(s):  
Cell Cultures ◽  
Low Cost ◽  
Fabrication Process ◽  
Cardiac Cell ◽  
Proof Of Concept ◽  
Electrode Arrays ◽  
Electrophysiological Analysis ◽  
Valid Proof ◽  
Cardiac Cell Cultures

We present an innovative fabrication process for the production of low cost fully-plastic flexible MEAs and prove that they are a valid proof-of-concept for a platform for the electrophysiological analysis of cardiac cell cultures.

Cell-line-dependent sorting of recombinant phytase in cell cultures of Medicago truncatula

2009 ◽  
Vol 36 (5) ◽  
pp. 431 ◽  
Author(s):  
Pablo González-Melendi ◽  
Ana Sofia Pires ◽  
Rita Abranches
Keyword(s):  
Recombinant Protein ◽  
Medicago Truncatula ◽  
Cell Cultures ◽  
Low Cost ◽  
Protein A ◽  
Subcellular Location ◽  
Efficient System ◽  
Recombinant Product ◽  
Transgenic Lines ◽  
Recombinant Phytase

Plant cell cultures as platforms for recombinant protein production are favoured over other systems because they combine the benefits of plants (low cost of production, low biosecurity risk, conserved post-translational modifications) with those of controlled cell cultures. However, many factors that affect the correct synthesis and accumulation of the recombinant product still need to be determined; in particular, the trafficking route of the recombinant proteins is poorly understood. Suspension cell cultures of Medicago truncatula Gaertn. have been shown to offer a viable and highly efficient system for the production of a model glycoprotein – phytase from the fungus Aspergillus niger Tiegh. The present study investigated subcellular protein sorting by immunogold detection of recombinant phytase with an electron microscope in four independent Medicago cell cultures expressing phytase. Two lines contained a C-terminal KDEL targeting signal for retention in the endoplasmic reticulum (ER), and the other two did not and were expected to travel through the secretory route; a high and low expressor were examined for each variant of the protein. A differential subcellular location of phytase was found in the four transgenic lines studied. These differences account not only for the version of the recombinant protein (secreted or retained in the ER), but also for the different expression levels.

scholarly journals A low-cost method to test cytotoxic effects of Crotalus vegrandis (Serpentes: Viperidae) venom on kidney cell cultures

2005 ◽  
Vol 47 (3) ◽  
pp. 147-152 ◽  
Author(s):  
María E. Girón ◽  
Irma Aguilar ◽  
Lisandro Romero ◽  
Elda E. Sánchez ◽  
John c. Pérez ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
Renal Cortex ◽  
Smooth Muscle Actin ◽  
Low Cost ◽  
Colorimetric Method ◽  
Vero Cells ◽  
Renal Lesion ◽  
Crude Venom

The pathogenesis of the renal lesion upon envenomation by snakebite has been related to myolysis, hemolysis, hypotension and/or direct venom nephrotoxicity caused by the venom. Both primary and continuous cell culture systems provide an in vitro alternative for quantitative evaluation of the toxicity of snake venoms. Crude Crotalus vegrandis venom was fractionated by molecular exclusion chromatography. The toxicity of C. vegrandis crude venom, hemorrhagic, and neurotoxic fractions were evaluated on mouse primary renal cells and a continuous cell line of Vero cells maintained in vitro. Cells were isolated from murine renal cortex and were grown in 96 well plates with Dulbecco's Modified Essential Medium (DMEM) and challenged with crude and venom fractions. The murine renal cortex cells exhibited epithelial morphology and the majority showed smooth muscle actin determined by immune-staining. The cytotoxicity was evaluated by the tetrazolium colorimetric method. Cell viability was less for crude venom, followed by the hemorrhagic and neurotoxic fractions with a CT50 of 4.93, 18.41 and 50.22 µg/mL, respectively. The Vero cell cultures seemed to be more sensitive with a CT50 of 2.9 and 1.4 µg/mL for crude venom and the hemorrhagic peak, respectively. The results of this study show the potential of using cell culture system to evaluate venom toxicity.

Latrine desludging pump development using a simple test for simulant strength: A case study from Uganda

2015 ◽  
Vol 5 (4) ◽  
pp. 620-624 ◽  
Author(s):  
J. T. Radford ◽  
S. Malinga ◽  
G. Drummond ◽  
H. Atayo ◽  
A. B. Whitesell ◽  
...  
Keyword(s):  
Low Cost ◽  
Field Trials ◽  
Simple Test ◽  
Average Flow Rate ◽  
Average Flow ◽  
Physical Strength ◽  
Technical Performance ◽  
First Time ◽  
Faecal Sludge

A simple and low-cost test to measure the physical strength of faecal sludge simulants is presented and used in the development of improved desludging pumps in Kampala, Uganda. The technical performance of two variants of the Gulper manual pump have for the first time been quantitatively assessed under controlled conditions. The time taken to prime and the average flow rate in the following 30 seconds are reported for both pumps on two different strengths of faecal sludge, demonstrating a distinct improvement in the redesigned Gulper II. This pump is now undergoing field trials across East Africa with a view to it being marketed and sold to pit-emptying entrepreneurs by Sanitation Solutions Group.

Constant Volume and Constant Pressure Respirometry

2018 ◽  
pp. 5-14
Author(s):  
John R. B. Lighton
Keyword(s):  
Oxygen Consumption ◽  
Cell Cultures ◽  
Constant Pressure ◽  
Low Cost ◽  
Constant Volume ◽  
Metabolic Rates

This chapter describes constant pressure (Gilson) and constant volume (Warburg) respirometry—long-established techniques that are still capable of accurate results. These are useful for measuring the metabolic rates of small organisms, cell cultures, and biochemical preparations. In Gilson respirometry, oxygen consumption is measured by reducing the volume of a sealed system to maintain a constant pressure. In Warburg respirometry, oxygen consumption is measured by quantifying the accompanying drop in the pressure of a sealed system, the volume of which is known. The theory and practical details of implementing both techniques are described in detail. Finally, a more modern, computerized alternative to Warburg respirometry is introduced that can be implemented at low cost.

Low-Cost and Modularized Test Bench for Focal Plane Array

2014 ◽  
Vol 981 ◽  
pp. 46-49
Author(s):  
Xi Qu Chen ◽  
Qiang Lv
Keyword(s):  
Signal Processing ◽  
Power Supply ◽  
Focal Plane ◽  
Test Bench ◽  
Low Cost ◽  
Focal Plane Array ◽  
Focal Plane Arrays ◽  
Simple Test ◽  
Optical Part ◽  
Set Up

In this paper, a low-cost and modularized test bench for microbolometric focal plane array is proposed. Based on the analysis of driving microbolometric focal plane array, we have set up the simple test bench. The test bench consists of four major modules: optical part, driving sequence timer, power supply and signal processing board, and data analyzer. Each module in the test bench is reconfigurable and the driving sequence timer is programmable in system. The proposed test bench is low-cost and has been applied to practical microbolometric focal plane arrays in our laboratory.

scholarly journals SpheroidPicker: An Automated 3D cell culture manipulator robot using deep learning

2020 ◽  
Author(s):  
Istvan Grexa ◽  
Akos Diosdi ◽  
Andras Kriston ◽  
Nikita Moshkov ◽  
Maria Harmati ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
High Throughput Screening ◽  
Ex Vivo ◽  
Low Cost ◽  
3D Cell Culture ◽  
Experimental Conditions ◽  
The Past ◽  
3D Cell Cultures ◽  
Manipulator Robot

AbstractRecent statistics report that more than 3.7 million new cases of cancer occur in Europe yearly, and the disease accounts for approximately 20 % of all deaths. High-throughput screening of cancer cell cultures has dominated the search for novel, effective anticancer therapies in the past decades. Recently, ex vivo 3D cell cultures from the patient’s own cancer cells have gained importance. We recently evaluated the major advancements and needs of the 3D cell cultures screening field, and we concluded that strictly standardized sample preparation is the most desired development. Here we propose an artificial intelligence-guided low-cost 3D cell culture delivery system. It consists of a light microscope, a micromanipulator, a syringe pump, and a controller computer. The system performs morphology-based feature analysis on spheroids and transfers the most appropriate ones between various sample holders. It can select the samples from standard sample holders, including Petri dishes and microwell plates, and then transfer them to a variety of holders up to 384 well plates. The device performs reliable semi- and fully automated spheroid transfer. This results in highly controlled experimental conditions and eliminates non-trivial side effects of sample variability that is a key aspect towards next-generation precision medicine.

Isolation of Mycoplasma hyorhinis and Mycoplasma fermentans from cell cultures

1980 ◽  
Vol 8 (4) ◽  
pp. 243-254 ◽  
Author(s):  
Anna A. Polak-Vogelzang ◽  
R. Reygers ◽  
F.E.N. Hekkens
Keyword(s):  
Cell Cultures ◽  
Mycoplasma Hyorhinis ◽  
Mycoplasma Fermentans
Sign in / Sign up

Export Citation Format

Share Document