Development of a New LC-MS/MS Screening Method for Detection of 120 NPS and 43 Drugs in Blood

In the last few years, liquid chromatography coupled with mass spectrometry (LC/MS) has been increasingly used for screening purposes in forensic toxicology. These techniques have the advantages of low time/resource-consuming and high versatility and have been applied in numerous new multi-analytes methods. The new psychoactive substance (NPS) phenomenon provided a great impulse to this wide-range approach, but it is also important to keep the attention on “classical” psychoactive substances, such as benzodiazepines (BDZ). In this paper, a fully validated screening method in blood for the simultaneous detection of 163 substances (120 NPS and 43 other drugs) by a dynamic multiple reaction monitoring analysis through LC-MS/MS is described. The method consists of a deproteinization of 200 µL of blood with acetonitrile. The LC separation is achieved with a 100 mm long C18 column in 35 min. The method was very sensitive, with limits of quantification from 0.02 to 1.5 ng/mL. Matrix effects did not negatively affect the analytical sensitivity. This method proved to be reliable and was successfully applied to our routinary analytical activity in several forensic caseworks, allowing the identification and quantification of many BDZs and 3,4-methylenedioxypyrovalerone (MDPV).

Identification of 2C-B in Hair by UHPLC-HRMS/MS. A Real Forensic Case

The analysis of drugs of abuse in hair and other biological matrices of forensic interest requires great selectivity and sensitivity. This has been traditionally achieved through target analysis, using one or more analytical methods that include different preanalytical stages, and more complex procedures followed by toxicological laboratories. There is no exception with 2C-series drugs, such as 2C-B, a new psychoactive substance (NPS), which use has emerged and significantly increased, year by year, in the last decades. Continuously new analytical methods are required to selectively detect and identify these new marketed substances at very low concentrations. In this case report, one former case of a polydrug consumer (charged of a crime against public health in Spain) was reanalyzed in hair matrix. In this reanalysis, 2C-B has been positively detected and identified using liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS/MS). The most selective analytical UHPLC-HRMS/MS method alongside a universal and simpler pretreatment methodology has opened up more possibilities for the detection of substances of different chemical structure and optimization of different HRMS/MS detection approaches allowing the identification of 2-CB in the hair of a real forensic case.

Effect of Unobserved Contextual Factors on the Prevalence of New Psychoactive Substance Use in Thailand

Objective: To explore the associations of characteristics of the cluster environment with new psychoactive substances (NPS) use in Thailand. Materials and Methods: A cross-sectional study was done on 30,411 Thai people, stratified in multi-stage cluster sampling. Trained interviewers conducted a semi-structured interview in 2016. A multilevel binary logistic regression model was employed to estimate the effects of the cluster environment on the NPS use. Results: NPS was widely used. The past month prevalence was 13.6%, and habitual use was 4.4%. Clustering significantly affects the spread of NPS use. Controlling for cluster effects, a likelihood of current NPS use was associated with elementary education, occupation, and reported health problems. While a higher likelihood of habitual NPS use was associated with elementary education, employed, and perceived mental health problem. Conclusion: NPS use was influenced by characteristics of the cluster environment. Therefore, demand reduction strategies should be designed based on a consideration of the impact of cluster context on NPS use behaviors. Keywords: Cluster effect, New psychoactive substance, NPS, Thailand