The present experiment aimed to gain more information on the effect of limb nonuse on the cognitive level of actions and, more specifically, on the content of the motor program used for grasping an object. For that purpose, we used a hand-grasping laterality task that is known to contain concrete information on manipulation activity. Two groups participated in the experiment: an immobilized group, including participants whose right hand and arm were fixed with a rigid splint and an immobilization vest for 24 hours, and a control group, including participants who did not undergo the immobilization procedure. The main results confirmed a slowdown of sensorimotor processes, which is highlighted in the literature, with slower response times when the participants identified the laterality of hand images that corresponded to the immobilized hand. Importantly, the grip-precision effect, highlighted by slower response times for hands grasping a small sphere versus a large sphere, is impaired by 24 hours of limb nonuse. Overall, this study provided additional evidence of the disengagement of sensorimotor processes due to a short period of limb immobilization.