ICESat-2 Marine Bathymetry: Extraction, Refraction Adjustment and Vertical Accuracy as a Function of Depth in Mid-Latitude Temperate Contexts

Nearshore bathymetric data are used in many coastal monitoring applications, but acquisition conditions can be challenging. Shipborne surveys are prone to the risk of grounding in shallow waters, and scheduled airborne surveys often fail to coincide with optimal atmospheric and water conditions. As an alternative, since its launch in 2018, ICESat-2 satellite laser profile altimetry data provide free and readily available data on a 91-day repeat cycle, which may contain incidental bathymetric returns when suitable environmental conditions prevail. In this paper, the vertical accuracy of extracted, refraction-adjusted ICESat-2 nearshore marine bathymetric data is evaluated at four test sites in a Northern hemisphere, temperate latitude location. Multiple ICEsat-2 bathymetric values that occurred in close horizontal proximity to one another were averaged at a spatial scale of 1 m and compared with Multibeam Echosounder bathymetric survey data and Global Navigation Satellite System reference data. Mean absolute errors of less than 0.15 m were observed up to depths of 5 m, with errors of less than 0.24 m (to 6 m), 0.39 m (to 7 m) and 0.52 m (to 10 m). The occurrence of larger bathymetric errors with depth, which increase to 0.54 m at maximum photon depths of 11 m, appears to be primarily related to reduced numbers of geolocated photons with depth. The accuracies achieved up to 6 m suggest that the manual extraction, refraction adjustment and bathymetric filtering steps were effective. Overall, the results suggest that ICESat-2 bathymetric data accuracy may be sufficient to be considered for use in nearshore coastal monitoring applications where shipborne and airborne bathymetric data might otherwise be applied.

Functional Diversity Facilitates Stability Under Environmental Changes in an Outdoor Microalgal Cultivation System

Functionally uniform monocultures have remained the paradigm in microalgal cultivation despite the apparent challenges to avoid invasions by other microorganisms. A mixed microbial consortium approach has the potential to optimize and maintain biomass production despite of seasonal changes and to be more resilient toward contaminations. Here we present a 3-year outdoor production of mixed consortia of locally adapted microalgae and bacteria in cold temperate latitude. Microalgal consortia were cultivated in flat panel photobioreactors using brackish Baltic Sea water and CO2 from a cement factory (Degerhamn, Cementa AB, Heidelberg Cement Group) as a sustainable CO2 source. To evaluate the ability of the microbial consortia to maintain stable biomass production while exposed to seasonal changes in both light and temperature, we tracked changes in the microbial community using molecular methods (16S and 18S rDNA amplicon sequencing) and monitored the biomass production and quality (lipid, protein, and carbohydrate content) over 3 years. Despite changes in environmental conditions, the mixed consortia maintained stable biomass production by alternating between two different predominant green microalgae (Monoraphidium and Mychonastes) with complementary tolerance to temperature. The bacterial population was few taxa co-occured over time and the composition did not have any connection to the shifts in microalgal taxa. We propose that a locally adapted and mixed microalgal consortia, with complementary traits, can be useful for optimizing yield of commercial scale microalgal cultivation.

Interspecific variation in heat tolerance and evaporative cooling capacity among sympatric temperate-latitude bats

We tested the hypothesis that interspecific variation in chiropteran heat tolerance and evaporative cooling capacity is correlated with day-roost microclimates, using three vespertilionid bats that occur sympatrically during summer in Saskatchewan, Canada. We predicted that hoary bats (Lasiurus cinereus Palisot de Beauvois, 1796; ~ 22 g) would have higher heat tolerance than little brown (Myotis lucifugus Le Conte, 1831; ~ 7 g) and silver-haired bats (Lasionycteris noctivagans Le Conte, 1831; ~ 13 g), as the latter two species roost in tree crevices/cavities that are more thermally buffered than the foliage roosts of hoary bats. We measured core body temperature (Tb; passive integrated transponder tags), evaporative water loss and resting metabolic rate (flow-through respirometry), while exposing individuals to a stepped profile of increasing air temperature (Ta) from ~ 30 °C in ~ 2 °C increments. Experiments were terminated when individuals became hyperthermic (Tb ≈ 42.5 °C), with maximum Ta (Ta,max) ranging from 42.0 °C – 49.7 °C. As predicted, hoary bats had the highest heat tolerance and evaporative cooling capacity, reaching Ta,max ~ 2.4 °C and 1.2 °C higher than little brown and silver-haired bats, respectively. Our results are consistent with the hypothesis that heat tolerance of bats is correlated with roost microclimates, although interspecific variation in body mass and phylogeny may confound these conclusions.

Mate Choice in Double-Breeding Female Great Tits (Parus Major): Good Males or Compatible Males

Producing two broods within the same season may be a good strategy by which short-lived species can maximize reproductive success. To produce two clutches in the same breeding season and to ensure offspring quality, choosing a good mate is important for females. Previous studies on double breeding focused on the associated influencing factors, and few studies examined how females choose social mates. Good genes and genetic compatibility are the two main hypotheses of the genetic benefit that females obtain from choosing mates. Uncovering the method used in mate choice for genetic benefits adopted by double-breeding females would provide a better understanding of the life history and rules of female choice. The great tit is an optionally double-breeding species in temperate-latitude populations. Here, we used a dataset for a Chinese population monitored between 2014 and 2016 to test two hypotheses on double-breeding female mate choice. A total of 30.1% of the breeding pairs initiated second breeding attempts, always remating with the same mate. The date of the first egg of the first brood did not affect initiation of a second brood, and female individual heterozygosity slightly influenced initiation of a second breeding. Female great tits choose males with both compatible genes and good genes in double-breeding mating. Double-breeding females prefer males with large breast stripes, high heterozygosity, and lower relatedness, while tarsus length, repertoire size, and individual F are not the main factors considered by females when selecting males for double breeding. The number of offspring of the first clutch did not affect the pairing status of male great tits in double breeding. The genetic quality of offspring from double-breeding pairs was higher than that of those from single-breeding pairs (higher heterozygosity and lower individual F). Taken together, our results showed that double breeding female great tits adopt multiple methods for genetic benefits to choose mates.

Early−Middle Devonian brachiopod provincialism and bioregionalization at high latitudes: A case study from southwestern Gondwana

The paleobiogeography of Early−Middle Devonian (Pragian−Eifelian) brachiopods from West Gondwana was assessed to determine any potential controls (regional climatic differences or global eustasy) that may have driven bioregionalization. The Pragian−Eifelian interval of West Gondwana was examined because work by previous authors suggested that this was a period when regionally extensive areas of provincialism among marine invertebrates were present and most pronounced. Factors of particular interest in this study were the controls over brachiopod bioregionalization at high (60°−90°) southern latitudes, which the Malvinokaffric Realm is thought to have entirely occupied. A large presence-absence data matrix was compiled consisting of the occurrences of 206 genera from 17 localities across West Gondwana, and an array of multivariate methods (cluster analysis, nonmetric multidimensional scaling, and network analysis) was employed to assess regional bioregionalization trends. The results of our study suggest that regional climatic differences brought on by latitudinal effects were the determining driver for bioregionalism of brachiopods during the Pragian−Eifelian, and these trends were coincident with a global cooling period during the Early−Middle Devonian. Our study further suggests that of the three regional paleobiogeographic realms thought to be present in West Gondwana during the Early−Middle Devonian (Eastern Americas, Old World, and Malvinokaffric), only the Malvinokaffric Realm is valid as a single biogeographic area. Its area, however, is reduced; it is interpreted to have been a second-order biogeographic area and not a first-order area as suggested by previous authors. Given these factors, we suggest a new demonym for this area, the “Malvinoxhosan,” given the racially charged connotations of “Malvinokaffric.” We present a new biogeographic framework for West Gondwana that is free of preconceived biogeographic area and rank biases, with the understanding that a more globally expansive study should be undertaken to elucidate these areas and their rank within the correct hierarchy. Two first-order paleobiogeographic areas are recognized in West Gondwana and are named according to their latitudinal extent, namely, the high-latitude (60°S−90°S) and temperate-latitude (30°S−60°S) bioregions. The temperate-latitude bioregion consists of a single second-order paleobiogeographic area, the “Colombian−West African” bioregion. Two second-order bioregions are present in the high-latitude bioregion, namely, the Amazonian and Malvinoxhosan bioregions. Here, the Amazonian (∼50°S−70°S), compositionally, was an intermediate region between the Colombian−West African (∼30°S−50°S) and Malvinoxhosan (70°S−90°S) end members. Latitudinal effects may also have been responsible for dividing the Malvinoxhosan bioregion into two tentative third-order paleobiogeographic areas, namely, the Andeo−South African (∼70°S−80°S) and the Paraná (∼80°S−90°S) bioregions.

Draft Aphaenogaster genomes expand our view of ant genome size variation across climate gradients

Given the abundance, broad distribution, and diversity of roles that ants play in many ecosystems, they are an ideal group to serve as ecosystem indicators of climatic change. At present, only a few whole-genome sequences of ants are available (19 of >16,000 species), mostly from tropical and sub-tropical species. To address this limited sampling, we sequenced genomes of temperate-latitude species from the genus Aphaenogaster, a genus with important seed dispersers. In total, we sampled seven colonies of six species: Aphaenogaster ashmeadi, Aphaenogaster floridana, Aphaenogaster fulva, Aphaenogaster miamiana, Aphaenogaster picea, and Aphaenogaster rudis. The geographic ranges of these species collectively span eastern North America from southern Florida to southern Canada, which encompasses a latitudinal gradient in which many climatic variables are changing rapidly. For the six genomes, we assembled an average of 271,039 contigs into 47,337 scaffolds. The Aphaenogaster genomes displayed high levels of completeness with 96.1% to 97.6% of Hymenoptera BUSCOs completely represented, relative to currently sequenced ant genomes which ranged from 88.2% to 98.5%. Additionally, the mean genome size was 370.5 Mb, ranging from 310.3 to 429.7, which is comparable to that of other sequenced ant genomes (212.8–396.0 Mb) and flow cytometry estimates (210.7–690.4 Mb). In an analysis of currently sequenced ant genomes and the new Aphaenogaster sequences, we found that after controlling for both spatial autocorrelation and phylogenetics ant genome size was marginally correlated with sample site climate similarity. Of all examined climate variables, minimum temperature, and annual precipitation had the strongest correlations with genome size, with ants from locations with colder minimum temperatures and higher levels of precipitation having larger genomes. These results suggest that climate extremes could be a selective force acting on ant genomes and point to the need for more extensive sequencing of ant genomes.

Modern carbonate formation in low-mineralized flowing waters

Continental carbonate formation in low-mineralized flowing waters of temperate latitude is rare phenomenon. Taking this into account a study was conducted of crustate and stalactite-like authigenic calcite aggregates formed in the thalwegs of the ravines of the Volga Upland. According to chemical and isotopic analysis, modern carbonates are formed from poorly mineralized, meteoric waters of medium hardness, hydrocarbonate-magnesium-calcium hydrochemical type. It has been established that newly formed carbonate aggregates are characterized by the predominance of lightweight carbon and oxygen in the crystal structure. The osmotic hypothesis of the formation of lime-calcite aggregates is proposed.

Glendonite occurrences in the Tremadocian of Baltica: first Early Palaeozoic evidence of massive ikaite precipitation at temperate latitude

The Tremadocian (Early Ordovician) is currently considered a time span of greenhouse conditions with tropical water surface temperature estimates, interpolated from oxygen isotopes, approaching 40°C. In the high-latitude Baltoscandian Basin, these data are in contrast with the discovery of glendonite, a pseudomorph of ikaite (CaCO3·6H2O) and valuable indicator of near-freezing bottom-water conditions. The massive precipitation of this climatically sensitive mineral is associated with transgressive conditions and high organic productivity. Surprisingly, the precipitation of glendonite is contemporaneous with the record of conodonts displaying low δ18O values, which would suggest high temperatures (>40°C) in the water column. Therefore, the early Tremadocian sediments of Baltoscandia contain both “greenhouse” pelagic signals and near-freezing substrate indicators. This apparent paradox suggests both the influence of isotopically depleted freshwater yielded by fluvial systems, and the onset of sharp thermal stratification patterns in a semi-closed basin, which should have played an important role in moderating subpolar climates and reducing latitudinal gradients.

Draft Aphaenogaster genomes expand our view of ant genome size variation across climate gradients

ABSTRACTGiven the abundance, broad distribution, and diversity of roles that ants play in many ecosystems, they are an ideal group to serve as ecosystem indicators of climatic change. At present, only a few whole-genome sequences of ants are available (19 of > 16,000 species), mostly from tropical and sub-tropical species. To address this limited sampling, we sequenced genomes of temperate-latitude species from the genus Aphaenogaster, a genus with important seed dispersers. In total, we sampled seven colonies of six species: A. ashmeadi, A. floridana, A. fulva, A. miamiana, A. picea, and A. rudis. The geographic ranges of these species collectively span eastern North America from southern Florida to southern Canada, which encompasses a latitudinal gradient in which many climatic variables are changing rapidly. For the six genomes, we assembled an average of 271,039 contigs into 47,337 scaffolds. The mean genome size was 370.5 Mb, ranging from 310.3 to 429.7, which is comparable to that of other sequenced ant genomes (212.8 to 396.0 Mb) and flow cytometry estimates (210.7 to 690.4 Mb). In an analysis of currently sequenced ant genomes and the new Aphaenogaster sequences, we found that after controlling for both spatial autocorrelation and phylogenetics ant genome size was marginally correlated with sample site climate similarity. Of all examined climate variables, minimum temperature showed the strongest correlation with genome size, with ants from locations with colder minimum temperatures having larger genomes. These results suggest that temperature extremes could be a selective force acting on ant genomes and point to the need for more extensive sequencing of ant genomes.