Mistimed feeding disrupts circadian rhythms of male mating behavior and female preovulatory LH surges in mice

In rodents, eating at atypical circadian times, such as during the biological rest phase when feeding is normally minimal, reduces fertility. Prior findings suggest this fertility impairment is due, at least in part, to reduced mating success. However, the physiological and behavioral mechanisms underlying this reproductive suppression are not known. In the present study, we tested the hypothesis that mistimed feeding-induced infertility is due to a disruption in the normal circadian timing of mating behavior and/or the generation of pre-ovulatory luteinizing hormone (LH) surges (estrogen positive feedback). In the first experiment, male+female mouse pairs, acclimated to be food restricted to either the light (mistimed feeding) or dark (control feeding) phase, were scored for mounting frequency and ejaculations over 96 hours. Male mounting behavior and ejaculations were distributed much more widely across the day in light-fed mice than in dark-fed controls and fewer light-fed males ejaculated. In the second experiment, the timing of the LH surge, a well characterized circadian event driven by estradiol (E2) and the SCN, was analyzed from serial blood samples taken from ovariectomized and E2-primed female mice that were light-, dark-, or ad-lib-fed. LH concentrations peaked 2h after lights-off in both dark-fed and ad-lib control females, as expected, but not in light-fed females. Instead, the normally clustered LH surges were distributed widely with high inter-mouse variability in the light-fed group. These data indicate that mistimed feeding disrupts the temporal control of the neural processes underlying both ovulation and mating behavior, contributing to subfertility.

The Cost of Reproduction in a Cooperatively Breeding Mammal: Consequences of Seasonal Variation in Rainfall, Reproduction, and Reproductive Suppression

Biological investments, such as reproduction, are influenced by both biotic and abiotic factors and their interactions. The trade-off between reproduction and survival has been well established. Seasonally breeding species, therefore, may exhibit variations in these trade-offs, but there is a dearth of knowledge concerning this. This study investigated the physiological cost of reproduction (measured through oxidative stress) across seasons in the cooperatively breeding highveld mole-rat (Cryptomys hottentotus pretoriae), one of the few seasonal breeding mole-rats. Oxidative stress indicates elevated reactive oxygen species (ROS) levels, which can overwhelm antioxidant defences resulting in damaged proteins, lipids and DNA, which overall can reduce longevity and compromise reproduction. Oxidative markers such as total oxidant status (TOS-measure of total peroxides present), total antioxidant capacity (TAC), oxidative stress index (OSI), and malondialdehyde (MDA) are utilised to measure oxidative stress. In this study, breeding and non-breeding male (NBM) and female mole-rats were captured during the dry season (breeding period) and wet season (non-breeding period). There was an apparent cost of reproduction in the highveld mole-rat; however, the seasonality pattern to the cost of reproduction varied between the sexes. Breeding females (BFs) had significantly higher MDA during the breeding period/dry season in comparison to the non-breeding period/wet season; this is possibly a consequence of bearing and nursing offspring. Contrastingly, breeding males (BMs) showed increased oxidative damage in the non-breeding/wet season compared to the breeding/dry season, possibly due to increased activities of protecting their mating rights for the next breeding/dry season, but this was not significant. Interestingly, during the non-breeding period/wet season, non-breeding females (NBFs) are released from their reproductive suppression, which resulted in increases in TOS and OSI, which again indicated that just the mere ability to be able to breed results in a cost (oxidative stress). Therefore we can speculate that highveld mole-rats exhibited seasonal variation in redox balance brought about by variation in abiotic variables (e.g., rainfall), physiology and behaviour. We conclude that physiological changes associated with reproduction are sufficient to induce significant acute oxidative stress in the plasma of female highveld mole-rats, which become alleviated following transition to the non-breeding season/wet period suggesting a possible hormetic effect.

Filling in the holes: The reproductive biology of the understudied Mahali mole-rat (Cryptomys hottentotus mahali)

African mole-rats have provided great insight into mammalian evolution of sociality and reproductive strategy. However, some species have not received attention, and these may provide further insights into these evolutionary questions. The cooperatively breeding Mahali mole-rat (Cryptomys hottentotus mahali (Roberts, 1913)) is one such species. Body mass, reproductive-tract morphometrics, gonad histology, and plasma reproductive hormone concentrations were studied for breeding and non-breeding males and females over 1 year. This study aimed to discern if this species exhibits a seasonal or aseasonal breeding pattern and whether there is a relaxation of reproductive suppression at any point in the year in non-breeding animals. The pattern of reproductive relaxation during the wetter months is similar to other African mole-rat species. Interestingly, births and pregnant breeding females were recorded throughout the year, thus indicating an aseasonal breeding strategy, despite inhabiting a region that experiences seasonal rainfall. However, there were periods of the year favouring increased reproduction to enable an increased likelihood of offspring survival. This suggests that the Mahali mole-rat may be an opportunistic breeder possibly brought about by the benefits of living in a cooperatively breeding group and potentially moving into more arid environments that were previously unexploited by the genus Cryptomys Gray, 1864.

Emergency contraception using progestin drugs in domestic cats

Today, in conditions of strict quarantine, when it is not always possible to carry out surgery in cats, some methods of emergency drug contraception can be used. Over the years, many contraceptive medications have been developed for companion animals, but many secondary adverse effects have limited their use. However, in all species, the secondary adverse effects of progesterone-type drugs limit their use and vary depending on when treatment is given in relationship to the stage of the estrous cycle. An ideal non-surgical sterilant would be safe, effective, permanent, administered as a single injection and capable of being manufactured inexpensively. Contraceptives are used for cats, as a method of renewable fertility over time for breeding animals, as a method of suppressing sexual function for animals at risk for surgery, and to prevent over populations in stray cats. The use of non-surgical, safe and effective methods of suppression of sexual function in cats eliminates traumatization, anesthesia and complicated postoperative period in animals, which in some cases are impossible in the state of health, etc. The most common and affordable method of medical contraception is progestins, such as megestrol acetate, medroxyprogesterone acetate, proligestone (used orally or as a depot injection). These drugs are effective in inhibiting sexual function. Contraceptives for felids are available as implants, depot injections, and pills or liquid for oral administration, powder for mixing with food, or as vaccines. Regimens may differ according to the timing of first treatment, whether coinciding with the active reproductive period, preceding this period, or during anestrus. Ideally, the treatment regimen is initiated during anestrus to minimize the dose needed for reproductive suppression and to avert exposure to endogenous steroids that can cause lesions in reproductive organs.

Is the Mahali mole-rat (Cryptomys hottentotus mahali) a spontaneous or induced ovulator?

The Mahali mole-rat (Cryptomys hottentotus mahali (Roberts, 1913)) is a social, cooperatively breeding subterranean rodent that breeds aseasonally. Only one female in a colony breeds and the remaining females are reproductively suppressed. When the opportunity arises, these non-reproductive females disperse from the natal colony to escape reproductive suppression and pair up with an unrelated male to start a new colony. This study set out to determine whether female Mahali mole-rats are induced or spontaneous ovulators once separated from the reproductive suppression of the breeding female. Fifteen separated females were subjected to three treatments: housed separately without a male (A), allowed chemical, but not physical, contact with a vasectomised male (NPC), and placed in direct contact with a vasectomised male (PC). Urine was collected from all females under each treatment every 2 days for 40 days. Only females housed in the PC treatment exhibited heightened progesterone concentrations and corpora lutea of ovulation in the ovaries. Furthermore, males possessed epidermal spines on the shaft of the penises that may be used to stimulate the cervix of the female during copulation. These findings suggest that the Mahali mole-rat is an induced ovulator.

A test of oscillation in the human secondary sex ratio

Background and objectives The sex ratio of human birth cohorts predicts the health and longevity of their members. Most literature invokes natural selection in support of the argument that heritable tendencies to produce male or female offspring induce oscillation in the sex ratio and its sequelae. Tests of the argument remain exceedingly rare because they require vital statistics describing many generations of a population both unaffected by migration and exposed to an exogenous stressor virulent enough to change the sex ratio at birth. We contribute to the literature by using time-series modeling to detect oscillation in the best data currently available for such a test. Methodology We apply rigorous time-series methods to data describing Sweden from 1751 through 1830, a period when the population not only aged in place without migration, but also exhibited the effects of an Icelandic volcanic eruption including a historically low secondary sex ratio. That very low sex ratio should have induced oscillation if heritable mechanisms appear in humans. Results We detected oscillation in the ratio but not that predicted by heritable tendencies to produce males or females. We found peak-to-trough oscillation at 14 rather than the approximately 32 years expected from the heritable tendencies argument. Conclusions and implications Our findings suggest that mechanisms other than perturbation of heritable tendencies to produce males or females induce oscillation in the human secondary sex ratio. These other mechanisms may include reproductive suppression and selection in utero. LAY SUMMARY The male to female ratio in human birth cohorts predicts longevity but its variation over time remains unexplained. We test the long-held theory that the ratio oscillates due to heritable tendencies to produce males or females. We find oscillation, but it appears due to social processes rather than heritable mechanisms.

Ovulation mitigates fatty liver associated with reproductive suppression and oxidative stress in Damaraland mole-rats (Fukomys damarensis)

Oxidative damage is often linked to reproduction; however, reproducing female Damaraland mole-rats (Fukomys damarensis) exhibit a reduction in oxidative damage relative to their non-reproductive, anovulatory, cohorts. Specifically, liver concentrations of malondialdehyde, a biomarker for lipid peroxidation, are significantly lower in reproducing females. We examined liver histology in reproductive, anovulatory and recently ovulating non-reproductive females, demonstrating an accumulation of lipid droplets only in the livers of anovulatory females and no fibrosis, cell death or inflammatory infiltrates in any group. Our observations suggest that anovulatory females experience a form of non-alcoholic fatty liver disease, which is reversed once they commence ovulation. We propose hormonal interactions that may underlie our observations.