shenmai injection
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2022 ◽  
Author(s):  
Jing Wu ◽  
zhonghao li ◽  
xiaoke dong ◽  
siyuan yuan ◽  
jinmin liu ◽  
...  

Abstract Background: Acute ischemic stroke (AIS) and following reperfusion therapy-induced cerebral ischemia reperfusion (I/R) injury have been recognized as an important subject of cerebrovascular disease with high mortality. Oxidative stress is an important pathological process of cerebral I/R injury. microRNA-19a (miR-19a) is involved in I/R. As the organ protectant agent, Shenmai Injection (SMI) is widely used in the clinical treatment of cerebral infarction. Purpose: This study aims to explore whether SMI can reduce oxidative stress by regulating miR-19a, thereby treating I/R injury. Methods: The oxidative stress state of PC12 cells was induced by H2O2, and then the cells were cultured with SMI. The therapeutic effect of SMI was evaluated by detecting cellular superoxide dismutase (SOD), malondialdehyde (MDA) and other oxidative markers with the kit. Western blot, PCR, immunofluorescence and other techniques were used to elucidate the potential mechanism of SMI. Results: Cell viability assay results showed that SMI could improve the viability of PC12 cells stimulated by H2O2. Compared with the H2O2 group, after SMI treatment, the contents of MDA and reactive oxygen species (ROS) were significantly reduced, while the activity of SOD was significantly increased, and SMI could reduce apoptosis by increasing the content of adenosine 5'-triphosphate (ATP) in cells and enhancing the mitochondrial membrane potential (∆Ψm). Western blot and qRT-PCR results showed that these effects were partially achieved through the AMPK/Sirt1/PGC-1α pathway. The level of miR-19a was significantly increased in H2O2 group, and SMI could protect the cells by reducing miR-19a. Further investigated the target of miR-19a, and transfected cells with miR-19a mimic and inhibitor respectively. We found that AdipoR2 was a direct target of miR-19a, and miR-19a could inhibit AdipoR2/PI3K/Akt/mTOR pathway. Conclusion:SMI can activate AMPK/Sirt1/PGC-1α and AdipoR2/PI3K/Akt/mTOR pathways by reducing miR-19a levels, and protect PC12 cells stimulated by H2O2.


2021 ◽  
Author(s):  
Xiaonan Zhang ◽  
Yanyang Li ◽  
Wanqin Zhang ◽  
Qiujin Jia ◽  
Yaping Zhu ◽  
...  

Abstract Background: Shenmai Injection (SMI) is a patented Chinese medicine extract, has been widely used to treat myocardial damage caused by doxorubicin (DOX), but its underlying mechanisms remain elusive. The study aimed to explore the protective effect of SMI on myocardial injury caused by DOX in vivo.Methods: The male Sprague-Dawley (SD) rats received DOX (2mg/kg) tail vein injection every week for 4 weeks, with or without SMI and miR-30a agomir treatment for 2 weeks. The protective effect of SMI on myocardial injury caused by DOX has been determined by measuring rat body mass and general heart morphology, myocardial pathological changes, and serum markers. The myocardial pathological changes were observed by Van Gieson (VG) staining, the serum marker levels of myocardial injury were detected by ELISA, the myocardial cell apoptosis was observed by TUNEL assay and transmission electron microscope, and the expression of target protein was detected by Western Blot.Results: SMI treatment significantly reduced rat HMI and LVMI, reduced the levels of serum CK, LDH, cTnT, NT-proBNP, and also reduced the levels of serum sST2 and GDF-15, and reduced the expression of rat myocardial type I and type III collagen, which was effective reduce the fibrosis of myocardial collagen knot tissue and interstitial. The study further found that SMI can increase the expression of Bcl-2 protein, reduce the expression of Bax, Caspase-9, and Caspase-3 protein, and reduce the apoptotic index of cardiomyocytes. Conclusion: The potential mechanism of SMI on cardiomyocytes from apoptosis induced by the DOX may be attributed to the regulation of miR-30a/beclin 1.


2021 ◽  
Author(s):  
Xianbing Hou ◽  
◽  
Dandan Chen ◽  
Tongfei Cheng ◽  
Dan Wang ◽  
...  

2021 ◽  
Vol 139 ◽  
pp. 111582
Author(s):  
Xiaonan Zhang ◽  
Shichao Lv ◽  
Wanqin Zhang ◽  
Qiujin Jia ◽  
Lirong Wang ◽  
...  

Phytomedicine ◽  
2021 ◽  
pp. 153597
Author(s):  
You-Ping Wu ◽  
Sheng Zhang ◽  
Yan-Fei Xin ◽  
Li-Qiang Gu ◽  
Xiao-Zhen Xu ◽  
...  
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