e22512 Background: Differences in breast cancer screening exist between the NYC neighborhoods of East Harlem (EH) and Central Harlem (CH), and the Upper East Side (UES). Here we assess the relationship between six cancer beliefs and breast cancer screening among women in these neighborhoods. Methods: We include women aged ≥40 who responded to the Community Cancer Needs Survey between 2018-2019 and were eligible to undergo screening mammography within 2 years (“recommended screening”). All estimates use weighted data generated using raking techniques. We compared categorical variables using Chi-square tests & estimated odds ratios (OR) and 95% confidence intervals (CI) from logistic regression model associating beliefs and reported mammography receipt (results and covariates listed in Table). For each belief, we compared women who reported “agree” (combined strongly or somewhat agree) to those who “disagree” (combined strongly or somewhat disagree). Results: Of the weighted sample of 76,610 (41.3% CH, 34.4% EH, and 24.3% UES) women eligible to undergo screening mammography, 75.1%, 81.2%, and 90.3% of women in CH, EH, and UES, respectively reported recommended screening. There was no difference by neighborhood in prolonged (>2 years ago) screening intervals: 10.6% in CH, 7.9% in EH, and 9.8% in the UES, while never use was reported by 11.3% in CH, 7.6% in EH, and none in the UES (p=<0.0001). The table summarizes agreement between cancer beliefs and timely receipt of mammography. Conclusions: In this study, cancer beliefs are inconsistently associated with use of breast cancer screening across three NYC neighborhoods. Assessment of beliefs reveal important opportunities for breast cancer (and other cancer) prevention by promoting awareness of risk factors and screening in these communities.[Table: see text]