Tracking of the biochemical changes upon pleomorphic adenoma progression using vibrational microspectroscopy

Head and neck tumors can be very challenging to treat because of the risk of problems or complications after surgery. Therefore, prompt and accurate diagnosis is extremely important to drive appropriate treatment decisions, which may reduce the chance of recurrence. This paper presents the original research exploring the feasibility of Fourier transform infrared (FT-IR) and Raman spectroscopy (RS) methods to investigate biochemical alterations upon the development of the pleomorphic adenoma. Principal component analysis (PCA) was used for a detailed assessment of the observed changes and to determine the spectroscopic basis for salivary gland neoplastic pathogenesis. It is implied that within the healthy margin, as opposed to the tumoral tissue, there are parts that differ significantly in lipid content. This observation shed new light on the crucial role of lipids in tissue physiology and tumorigenesis. Thus, a novel approach that eliminates the influence of lipids on the elucidation of biochemical changes is proposed. The performed analysis suggests that the highly heterogeneous healthy margin contains more unsaturated triacylglycerols, while the tumoral section is rich in proteins. The difference in protein content was also observed for these two tissue types, i.e. the healthy tissue possesses more proteins in the anti-parallel β-sheet conformation, whereas the tumoral tissue is dominated by proteins rich in unordered random coils. Furthermore, the pathogenic tissue shows a higher content of carbohydrates and reveals noticeable differences in nucleic acid content. Finally, FT-IR and Raman spectroscopy methods were proposed as very promising methods in the discrimination of tumoral and healthy tissues of the salivary gland.

APLICAÇÃO DA ESPECTROSCOPIA FT-IR E RAMAN PARA CARACTERIZAÇÃO DE SOLO OBTIDO EM ESCAVAÇÃO NO SÍTIO ARQUEOLÓGICO TEMPLO DOS PILARES, MATO GROSSO DO SUL

O presente estudo buscou determinar através da técnica FT-IR e Raman, características do solo coletado no sítio arqueológico ‘Templo dos Pilares’ localizado no município de Alcinópolis. Através da análise de FT-IR, pode-se observar que os átomos de compostos orgânicos podem ter sua vibração com amplitude aumentada ao redor das ligações covalentes que os ligam, nesse processo cada mudança de nível de energia vibracional corresponde uma série de mudanças de níveis de energia rotacional e assim originando as bandas com diferentes comprimentos de ondas observadas no espectro.APPLICATION OF FT-IR AND RAMAN SPECTROSCOPY FOR CHARACTERIZATION OF SOIL OBTAINED IN EXCAVATION AT THE PILLARS-MS TEMPLE ARCHAEOLOGICAL SITE, MATO GROSSO DO SUL ABSTRACTThe present study sought to determine, using the FT-IR and Raman technique, characteristics of the soil collected at the archaeological site ‘Temple of the Pillars’ located in the municipality of Alcinópolis. Through the analysis of FT-IR, it can be observed that the atoms of compounds can have their vibration with increased amplitude around the connections of the bonds that connect them, in this process each change of level of vibrational energy corresponding to a series of level changes of rotational energy and thus originating the bands with different wavelengths observed in the spectrum.Keywords: FT-IR; Characterization; Archaeological.

Structural Polymorphism of Sorafenib Tosylate as a Key Factor in Its Solubility Differentiation

The presence of active pharmaceutical ingredients (APIs) in the forms of different polymorphic states can induce differences in their physicochemical properties. In the case of poorly soluble APIs, like the oncological drug sorafenib tosylate, small variations in solubility may result in large bioavailability differences. The control of its therapeutic dose is crucial from the effective pharmacotherapy point of view and the reduction of side effects. Therefore, this study aimed to assess the influence of sorafenib tosylate polymorphic forms on its solubility and, consequently, permeability, based on passive diffusion through membranes simulating the gastrointestinal tract (GIT) conditions. In the first part of the work, two crystalline forms of sorafenib tosylate were identified using the X-ray powder diffraction, FT-IR, and Raman spectroscopy. Subsequently, solubility studies were carried out. Both forms of sorafenib tosylate were insoluble in 0.1 N hydrochloric acid (HCl), in acetate buffer (pH 4.5), and in phosphate buffer (pH 6.8). Solubility (mg/mL) of form I and III of sorafenib tosylate in 0.1 N HCl + 1.0% SDS was 0.314 ± 0.006 and 1.103 ± 0.014, respectively, in acetate buffer pH 4.5 + 1.0% SDS it was 2.404 ± 0.012 and 2.355 ± 0.009, respectively, and in phosphate buffer pH 6.8 + 1.0% SDS it was 0.051 ± 0.005 and 1.805 ± 0.023, respectively. The permeability study was assessed using the parallel artificial membrane permeability assay (PAMPA) model. The apparent permeability coefficient (Papp—cm s−1) of form I and III in pH 1.2 was 3.01 × 10−5 ± 4.14 × 10−7 and 3.15 × 10−5 ± 1.89 × 10−6, respectively, while in pH 6.8 it was 2.72 × 10−5 ± 1.56 × 10−6 and 2.81 × 10−5 ± 9.0 × 10−7, respectively. Changes in sorafenib tosylate concentrations were determined by chromatography using the high-performance liquid chromatography (HPLC)–DAD technique. As a result of the research on the structural polymorphism of sorafenib tosylate, its full spectral characteristics and the possibility of using FT-IR and Raman spectroscopy for the study of polymorphic varieties were determined for the first time, and the HPLC method was developed, which is appropriate for the assessment of sorafenib solubility in various media. The consequences of various physicochemical properties resulting from differences in the solubility of sorafenib tosylate polymorphs are important for pre-formulation and formulation studies conducted with its participation and for the safety of oncological sorafenib therapy.

Identification, Interaction and Detection of Microplastics on Fish Scales (Lutjanus Gibbus)

Background: Microplastics are found to be one of the major emerging contaminants in the environment. Various environmental occurrences cause the macro plastics to degrade slowly into microplastics. Microplastics present in the water bodies may enter into the fish’s body through ingestion of food and also get adsorbed onto the surface of their gills or skin. Objective: Microplastics of polyethylene were chosen to investigate its sorption capacity on fish scales. Dispersion of polyethylene microplastics was studied by using Total dissolved solids meter. Using this dispersion, the sorption effect was studied and it revealed that the microplastics has the sorption ability on the fish scales. Method: Polyethylene microplastics were chosen to investigate its sorption capacity on fish scales of Lutjanus gibbus. The sorption effect of microplastics on fish scales were performed by using polyethylene microplastics obtained by bath sonication and the concentration was studied using Total dissolved solids meter. Using polyethylene microplastics dispersion, the sorption effect was carried out on the scales of Lutjanus gibbus for ten days at 8 oC. Sorption of microplastics on fish scales were characterized by FE-SEM, FT-IR, and Raman spectroscopy. Results: Polymer sorption was confirmed by using optical microscopy and FE-SEM. FT-IR and Raman spectroscopy confirmed the existence of polyethylene microplastics on the fish scale. Moreover, polyethylene microplastics sorption studies were also studied in different pH, various concentrations of NaCl and at different time intervals. Conclusions: We have synthesized microplastics from the bulk polyethylene by NaCl solution. This study confirmed the successful sorption of polyethylene microplastics on the fish scale. Our study revealed that marine water may be a suitable medium to facilitate the polymer sorption on aquatic animals/organisms.

Impact of Fluorination and Chlorination on the Electronic Structure, Topology and in-Plane Ring Normal Modes of Pyridine

Seven partially and fully fluorinated/chlorinated pyridines were investigated by means of FT-IR and Raman spectroscopy combined with quantum chemical calculations with the main aim to detect how the nature and...

Formation of glasses and glass ceramics in the TeO2–BaZrF6–NaPO3 system

Синтезированы новые стеклокристаллические трехкомпонентные системы TeO2–BaZrF6–NaPO3. Методами ИК-, КР-спектроскопии и рентгенофазового анализа изучено влияние соотношения компонентов в системе на фазовый состав и строение полученных стеклокерамик. The new glass-crystalline three-component systems of TeO2–BaZrF6–NaPO3 have been synthesized. The influence of the ratio of the components in the system on the phase composition and structure of the glass-ceramics obtained has been studied by IR and Raman spectroscopy and X-ray phase analysis.

Enhanced Failure Analysis (FA) of Organic Contamination Using Submicron Simultaneous IR and Raman Spectroscopy: Breakthrough Developments of Optical Photothermal IR (O-PTIR)

Rapid identification of organic contamination in the semi and semi related industry is a major concern for research and manufacturing. Organic contamination can affect a system or subsystem’s performance and cause premature failure of the product. As an example, in February 2019 the Taiwan Semiconductor Manufacturing Company (TMSC), a major semiconductor manufacturer, reported that a photoresist it used included a specific element which was abnormally treated, creating a foreign polymer in the photoresist resulting in an estimated loss of $550M [1].