Waxing and Waning Neuroimaging Abnormalities in Langerhans Cell Histiocytosis

Langerhans cell histiocytosis (LCH) is a rare disease in adults characterized by proliferation of CD1a+/CD207+ and S100 myeloid dendritic cells, forming granulomas.1 An inflammatory exudate is often found with “bean-shaped” nucleated histiocytes. We report a patient with multisystem (liver, lung and brain) LCH in whom neuroimaging abnormalities accumulated and partially regressed prior to definitive treatment.

ОСОБЛИВОСТІ СИМПТОМАТИКИ ПАРААНАЛЬНОГО АДЕНІТУ У СВІЙСЬКИХ КОТІВ В УМОВАХ ВЕТЕРИНАРНОГО ЦЕНТРУ «РЫЖИЙ КОТ»

У статті представлені результати клінічного об-стеження котів із метою діагностування параана-льного аденіту, що надходили на амбулаторний при-йом у ветеринарний центр «Рыжий кот». З’ясованостатеву, породну, вікову схильність до зазначеноїпатології. На основі зібраного анамнезу, даних клініч-ного обстеження та функціональної діагностикивстановлено патологічні зміни, що супроводжуютьдану патологію. Зокрема, встановили розтягненнязалоз, розвиток запального процесу, звуження вивід-них протоків, зміну кольору запального ексудату. The article presents the results of clinical evaluation of cats with the aim of paraanal adenitum diagnosing. The cats came to outpatient admission to veterinary centre «Red cat». Clarified sex, breed, age-related predisposition to this disease. On the basis of the collected anamnesis and clinical examination and functional diagnostics defined pathological changes that accompany this disease. In particular we defined the tensile glands, inflammation, narrowing of the excretory ducts, change of the inflammatory exudate color.

Effects of carprofen administered by different routes to control experimental uveitis in dogs

Efficacy of carprofen, administered by different routes, was studied in experimental uveitis in dogs. Anterior chamber paracenteses was accomplished at two different moments (M0 and M1), with a five hour interval between them. At M0 and M1, 0.2mL of aqueous humor was collected and quantitation of total protein and prostaglandin E2 (PGE2) were determined. Four groups were formed (n=8), which received carprofen at the end of M0, by the following routes: subcutaneous (GIm), subconjunctival (GII), and topical (GIII). A fourth group that received no treatment was instituted (Control). Conjunctival histopathology of the GII animals was performed. In all groups, values of protein and PGE2 significantly enhanced at M1; however, they did not significantly change among groups at M1. Inflammatory exudate of acute character and mild hemorrhage were seen at histopathology after carprofen administration. Carprofen was unable to inhibit PGE2 synthesis and the protein influx to the anterior chamber by any of the tested routes. However, the reduction of 44% in protein levels (topical) suggests that the agent can be used by this route as an adjuvant to control uveitis in dogs.

Effects of meloxicam administered by different routes to control experimental uveitis in dogs

Efficacy of meloxicam, administered by different routes was studied in experimental uveitis in dogs. Anterior chamber paracenteses was accomplished at two different moments (M0 and M1), with a five hour interval among them. At M0 and M1, 0.2mL of aqueous humor was collected and total protein and prostaglandin E2 (PGE2) quantitation was determined. Four groups were formed (n=5), which received meloxicam at the end of M0. by the following routes: subcutaneous (GIm), subconjunctival (GII), and topical (GIII). A fourth group that received no treatment was instituted (Control). Conjunctival histopathology of the GII was performed. Results were evaluated statistically (P≤0.05). In all groups, protein and PGE2 values enhanced significantly in M1. Protein and PGE2 values did not change significantly between groups at M1. Inflammatory exudate of acute character and mild hemorrhage were seen at histopathology, after meloxicam administration. Meloxicam were unable to inhibit PGE2 synthesis and the protein influx to the anterior chamber by any of the tested routes.

Characterization of the acute inflammatory response in the hybrid tambacu (Piaractus mesopotamicus male × Colossoma macropomum female) (Osteichthyes)

This work evaluated the acute inflammatory response induced by injections of 0.5 mL saline solution (control), 500 µg carrageenin and 0.5 mL thioglycollate 3% in the swim bladder of juvenile tambacu hybrid. Fish were distributed in three treatments, three replications and acclimated for a period of 10 days before assay. The cell characterization from the inflammatory exudate was performed in Giemsa and PAS stained smears. Carrageenin, injected in fish, showed an increase on the total number of cells in the inflammatory exudate when compared to saline and thioglycollate injected. Whereas, for carrageenin-injected fish, the percentage of thrombocyte was higher than thioglycollate. On the other hand, granulocyte percentage in thioglycollate-injected fish was higher than the ones injected using carrageenin. Carrageenin provoked the highest migration of macrophage to the inflammatory site. The PAS method confirmed the presence of three types of granulocytes: eosinophilic granular cell (EGC) type 1 with the characteristics of a special granulocytic cell commonly found in the circulating blood; EGC type 2 shorter than the last one and neutrophil. This study contributes to a better understanding of the inflammatory response and infectious processes in native fish.

Dealcoholized red and white wines decrease oxidative stress associated with inflammation in rats

In vitroexperiments have demonstrated that polyphenols exhibit antioxidant and anti-inflammatory activities. The present study was designed to test whether dealcoholized red (DRW) and white (DWW) wines can decrease the oxidative stress associated with inflammationin vivo. Rats were fed for 15 d either a control diet or one supplemented with DRW or DWW. Finally, a granuloma was induced by subcutaneous administration of carrageenan. Although DRW showed higher antioxidant activityin vitrothan DWW, both wines decreased the number of cells recruited into the granuloma pouch. Malondialdehyde decreased in plasma and inflammatory exudate from rats fed with DRW- and DWW-rich diets. Moreover, the concentration of NO increased in exudate, which correlates with the increase in the citrulline:arginine ratio. Polymorphonuclear leucocytes from the inflammatory exudate of rats fed dealcoholized wines showed decreased superoxide anion (O2∙−) production and increased NO productionex vivo. This change in NO production resulted from increased expression and activity of inducible NO synthase (EC 1.14.13.39). Moreover, the up regulation of cyclo-oxygenase-2 (EC 1.14.99.1) protein expression observed in rats fed the DRW-rich diet was not related to a direct effect of NO. The present results indicate that the non-alcoholic compounds of wines not only improve antioxidant status in an inflammatory situation, but also limit cell infiltration, possibly through a decrease in O2∙−and an increase in NO production.