Bone is a highly vascularized tissue. However, despite the importance of appropriate circulationfor bone health, regulation of bone blood flow remains poorly understood. Invasive animalstudies suggest that the sympathetic activity plays an important role in bone flow control.However, it remains unknown if bone vasculature evidences robust vasoconstriction in responseto sympathoexcitatory stimuli. Here, we characterized bone blood flow in young healthyindividuals (N=13,(4F)) in response to isometric handgrip exercise (IHE) and cold pressor test(CPT). These provide a strong stimulus for active vasoconstriction in the inactive muscle, andperhaps also in the bone. During sustained IHE to fatigue and CPT, we measured blood pressure,whole leg blood flow, and tibial perfusion using near-infrared spectroscopy. Tibia perfusion wasdetermined as oxy- and deoxy-hemoglobin. For both stimuli, tibial metabolism remainedconstant (i.e., no change in deoxyhemoglobin) and thus tibial arterial perfusion was representedby oxyhemoglobin. During IHE, oxyhemoglobin declined (beginning -0.20±1.04μM; end -1.13±3.71μM, both p<0.01) slower than whole leg blood flow (beginning -0.85±1.02cm/s; end -2.72±1.64cm/s, both =p<0.01). However, during CPT, both oxyhemoglobin (beginning -0.46 ±1.43μM; end -0.60±1.59μM, both p<0.01) and whole leg blood flow (beginning -1.52±1.63 cm/s;end -0.69±1.51cm/s, both p<0.01) declined with a similar time course, even though themagnitudes of decline were smaller than during IHE. These responses are likely due the differenttime courses of sympathetically mediated vasoconstriction in bone and muscle. These resultsindicate that sympathetic innervation of the bone vasculature serves a functional role in thecontrol of flow in young healthy individuals.