Optimization of Noise Transfer Path Based on the Composite Panel Acoustic and Modal Contribution Analysis

The noise of a cab directly affects the comfort and labor efficiency of the operators. The optimization of the structure-borne transmission path can obviously reduce the cab noise. The method of panel acoustic contribution analysis (PACA) is used to reduce structure noise. However, most studies only consider the panel acoustic contribution of a single frequency, without considering the contribution of major frequencies synthesis to confirm the optimized panels. In this paper, a novel method is proposed based on composite panel acoustic and modal contribution analysis and noise transfer path optimization in a vibro-acoustic model. First, the finite element model (FEM) and the acoustic model are established. Based on the acoustic transfer vector (ATV) method, a composite panel acoustic contribution analysis method is proposed to identify the panels affecting the noise of the field point. Combined with the modal acoustic contribution of the modal acoustic transfer vector (MATV) method, the noise field point is confirmed in the area which has the most significant influence. Second, the optimization algorithm NLOPT which is a nonlinear optimization is applied to design the areas. The noise transfer path optimization with vibroacoustic coupling response can quickly determine the optimal thickness of the panels and reduce low-frequency noise. The effectiveness of the proposed method is applied and verified in an excavator cab. The sound pressure level (SPL) the driver’s right ear (DRE) decreased obviously. The acoustic analysis of the composite panel acoustic contribution and modal acoustic contribution can more accurately recognize an optimized area than the traditional PACA. This method can be applied in the optimization of the structure-borne transmission path for construction machinery cab and vehicle body.

Computing Radiated Sound Power using Quadratic Power Transfer Vector (QPTV)

Pressure Acoustic Transfer Functions or Vectors (PATVs) relate the surface velocity of a structure to the sound pressure level at a field point in the surrounding fluid. These functions depend only on the structure geometry, properties of the fluid medium (sound speed and characteristic density), the excitation frequency and the location of the field point, but are independent of the surface velocity values themselves. Once the pressure acoustic transfer function is computed between a structure and a specified field point, we can compute pressure at this point for any boundary velocity distribution by simply multiplying the forcing function (surface velocity) with the acoustic transfer function. These PATVs are usually computed by application of the Reciprocity Principle, and their computation is well understood. In this work, we present a novel way to compute the Velocity Acoustic Transfer Vector (VATV) which is a relation between the surface velocity of the structure and fluid particle velocity at a field point. To our knowledge, the computation of the VATV is completely new and has not been published in earlier works. By combining the PATVs and VATVs at a number of field points surrounding the structure, we obtain the Quadratic Power Transfer Vector (QPTV) that allows us to compute the sound power radiated by a structure for ANY surface velocity distribution. This allows rapid computation of the sound power for an arbitrary surface velocity distributions and is useful in designing quiet structures by minimizing the sound power radiated.

Construction of a Baculovirus Derivative to Produce Linearized Antheraea pernyi (Lepidoptera: Saturniidae) Multicapsid Nucleopolyhedrovirus Genomic DNA

In the Antheraea pernyi multicapsid nucleopolyhedrovirus (AnpeNPV)-based expression vector system, the frequency of homologous recombination events between wild-type AnpeNPV DNA and the transfer vector is low, resulting in a small amount of recombinant virus. Previous reports have indicated that linearized baculovirus DNA can increase the proportion of recombinant virus relative to the total progeny. To improve the recombination efficiency, we constructed a linearized derivative of AnpeNPV, referred to as AnpeNPVPhEGFP-AvrII, in which egfp flanked by AvrII restriction sites was located at the polyhedrin locus and driven by the polyhedrin promoter. Linear AnpeNPV DNA was obtained by the treatment of AnpeNPVPhEGFP-AvrII genomic DNA with AvrII endonuclease. The infectivity and recombinogenic activity between the linearized and circular viral DNA were evaluated by quantitative real-time polymerase chain reactions. We demonstrated that the linearized AnpeNPV DNA produced only small numbers of infectious budded viruses, accounting for approximately 4.5% of the budded virus production of wild-type AnpeNPV DNA in A. pernyi pupae. However, the linearized AnpeNPV DNA substantially increased recombinant virus production after cotransfection with an appropriate transfer vector; relative abundance of the recombinant virus was approximately 5.5-fold higher than that of the wild-type AnpeNPV DNA in A. pernyi pupae. The linearization of AnpeNPV DNA will facilitate the purification of recombinant viruses using the AnpeNPV-based expression vector system and the construction of an AnpeNPV-based bacmid system.

Construction of New Genetically Engineered Vaccine Strain for O-type Foot-and-Mouth Disease of Pig

Foot-and-mouth disease is an acute and highly contagious viral infectious disease. Although the foot and mouthdisease vaccine has been applied in some parts of the world since the beginning of the 20th century, the currentepidemic of foot and mouth disease in the world is still serious and constitutes an obstacle to the trade of animal andanimal products in the world. The porcine pseudorabies virus gene deletion strain PRV TK-/ gE-/ LacZ +, which isconstructed by our laboratory, has the advantages of good safety, large capacity and high recombination effi ciency. Inthis study, the artifi cial O-type foot-and-mouth disease P1 gene as an antigen gene, UbiP1 fused with ubiquitin (Ub)as another antigen gene to enhance cellular immunity, and then shRNA designed for FMD 3B gene and porcine IFN-[gamma] with antiviral and immune-regulatory eff ects. The two functional genes were constructed in turn to constructa transfer vector with four functional genes. The transfer vector was transfected into TK-/ gE- / LacZ+ cells with PRVdeletion vector, and the plasmids were purifi ed and identifi ed. The recombinant pseudorabies virus, which containsthe six functional genes of P1 gene, UbiP1, shRNA and IFN-γ, was obtained, which laid the foundation for furtherconstruction of new genetically engineered vaccine.