Statistical Integration of ‘Omics Data Increases Biological Knowledge Extracted from Metabolomics Data: Application to Intestinal Exposure to the Mycotoxin Deoxynivalenol

The effects of low doses of toxicants are often subtle and information extracted from metabolomic data alone may not always be sufficient. As end products of enzymatic reactions, metabolites represent the final phenotypic expression of an organism and can also reflect gene expression changes caused by this exposure. Therefore, the integration of metabolomic and transcriptomic data could improve the extracted biological knowledge on these toxicants induced disruptions. In the present study, we applied statistical integration tools to metabolomic and transcriptomic data obtained from jejunal explants of pigs exposed to the food contaminant, deoxynivalenol (DON). Canonical correlation analysis (CCA) and self-organizing map (SOM) were compared for the identification of correlated transcriptomic and metabolomic features, and O2-PLS was used to model the relationship between exposure and selected features. The integration of both ‘omics data increased the number of discriminant metabolites discovered (39) by about 10 times compared to the analysis of the metabolomic dataset alone (3). Besides the disturbance of energy metabolism previously reported, assessing correlations between both functional levels revealed several other types of damage linked to the intestinal exposure to DON, including the alteration of protein synthesis, oxidative stress, and inflammasome activation. This confirms the added value of integration to enrich the biological knowledge extracted from metabolomics.

Temporal binding as multisensory integration: Manipulating perceptual certainty of actions and their effects

It has been proposed that statistical integration of multisensory cues may be a suitable framework to explain temporal binding, that is, the finding that causally related events such as an action and its effect are perceived to be shifted towards each other in time. A multisensory approach to temporal binding construes actions and effects as individual sensory signals, which are each perceived with a specific temporal precision. When they are integrated into one multimodal event, like an action-effect chain, the extent to which they affect this event’s perception depends on their relative reliability. We test whether this assumption holds true in a temporal binding task by manipulating certainty of actions and effects. Two experiments suggest that a relatively uncertain sensory signal in such action-effect sequences is shifted more towards its counterpart than a relatively certain one. This was especially pronounced for temporal binding of the action towards its effect but could also be shown for effect binding. Other conceptual approaches to temporal binding cannot easily explain these results, and the study therefore adds to the growing body of evidence endorsing a multisensory approach to temporal binding.

Development of a list of terms in Brazilian Portuguese for the Qualitative Behaviour Assessment of broiler chickens

Qualitative Behaviour Assessment (QBA) is a methodological approach to assess the whole animal using terms to describe and quantify the emotionally expressive qualities of behaviour and identifying larger patterns of expressivity through multi-variate statistical integration. A key condition for the success of QBA is achieving a common understanding of the meaning of descriptive terms by raters. Based on this, our study aimed to develop a list of terms in Brazilian Portuguese for the QBA of broiler chickens (Gallus gallus domesticus), and to test this list by studying its inter- and intra-rater reliability. Fourteen experts participated in a workshop and developed a list of 25 QBA terms, and 40 undergraduates tested this list by scoring 18 video clips using a 125-mm visual analogue scale. Principal Component Analysis was used to analyse observers' scores. Principal Component (PC) 1 ranged from disturbed/frustrated to comfortable/lively, suggesting this PC may be interpreted in terms of emotional valence. PC2 ranged from calm/dull to agitated/active, suggesting this PC indicates the level of arousal/energy of the birds. Both PC1 and PC2 clip scores showed good inter- and intra-rater reliability. This study demonstrates the importance of producing QBA term lists bottom-up as opposed to merely translating pre-existing lists from the scientific literature. Results suggest the standardised Portuguese QBA term list developed in this study is reliable in assessing the expressive qualities of broiler behaviour; therefore, a next step is to test it on-farm with experienced raters and further refine it concerning terms related to poor welfare.

Origins of the Northern Selkups Based on Anthropological Data

The authors examine the origins of the Upper Taz Selkups based on craniology and dental anthropology. They are one of the least studied groups of the indigenous population of Western Siberia. Judging by historical and ethnolinguistic data, the Northern Selkups moved to the Upper Taz region in the 17th – 18th century. Anthropological materials of the Northern Selkups were first obtained only in 2013 and 2016 during the excavations of Kikki-Akki burial ground. Recorded according to archaeological data, the burial rite has direct analogies in Southern Selkups burial grounds of the 17th – 18th centuries, with the exception of the selected individual features of the Eastern Khanty traditions. The craniological sample from Kikki-Akki burial includes 21 skulls – 13 males and 8 females. The dental sample includes the teeth of 22 individuals – 10 male, 6 female and 6 children. During the study the authors examined the anthropological materials based on the method of description of dental and cranial morphology, performed statistical integration. Characteristics of the series were compared with the obtained data of West Siberian near-recent samples. The analysis of the data shows that the Vakh Khanty represent the closest analogy to the series from Kikki-Akki, but the female part of the craniological sampling has a strong resemblance to the groups of the Southern Selkups. The results confirm the available historical and ethnolinguistic data on their formation due to the resettlement of a part of the Southern Selkup group from the Ob River Basin to the north, i.e. to the upper reaches of the Taz River. Moreover, the results demonstrate that the Selkup appearance changed quite a lot in a short period of time (200–300 years) that passed since their migration. The Northern Selkups acquired a significant resemblance to the Vakh Khanty – the only population with which the Selkups could maintain marital relations during their resettlement from the Middle Ob River to the Taz River.