Effects of Witch Hazel Oil on Damaged Hair

Purpose: This study aims to investigate the effects of applying witch hazel oil on damaged hair.Methods: Adding 2, 4, and 6 g of witch hazel oil, applied to a sample of hairs bleached at level eight, produced formulations intended to enhance hair quality. This sample was compared with a cohort of damaged hair. Lightness (L*) value was measured using a color meter to identify changes in the brightness of the hair sample. In addition, tensile strength, absorbance using methylene blue, and glossiness were measured to verify improvement in damaged hair.Results: The mean L* values of the hairs in the sample were higher when compared with those of healthy hair. However, this effect declined with increased oil dosage. Moreover, no statistically significant difference was observed between damaged hair and the sample. After applying the oil, the mean values for tensile strength in the sample increased in comparison with those of the hair in the damaged hair cohort. However, significant differences were noted for hairs 8L(2), 8L(4), and 8L(6) from the sample. The mean values for absorbance decreased in all the hairs sampled, with significant differences noted for hairs 8L(2), 8L(4), and 8L(6) from the sample. Another measure is glossiness, the mean values of which increased for hairs 8L(2), 8L(4), and 8L(6) from the sample with significant differences.Conclusion: Significant differences in the mean values for tensile strength, absorbance, and glossiness were observed in hairs from the sample treated with varying doses of witch hazel oil, which indicates that the oil can improve the quality of damaged hair. However, we recommend that further studies be conducted to determine improvements in damaged hair using a variety of oils and natural extracts.

The Protective Effect of Hamamelis virginiana Stem and Leaf Extract on Fine Dust-Induced Damage on Human Keratinocytes

Witch hazel extracts have been used for decades as cosmetic ingredients in skin care products. Our present study aims to evaluate its potential in anti-pollution products using a previously reported in vitro model. Calcium is a universal second messenger, and we used human respiratory and skin cells to detect changes in intracellular Ca2+ concentrations upon particulate matter contact. Both an increase in pro-inflammatory markers and a decrease in tight junction proteins were confirmed, as previously reported. Witch hazel stem and leaf extract showed significant attenuation of Ca2+ response upon the challenge; it displayed systematic regulations of the signal generator, PAR-2; a pro-inflammatory marker, NF-κB; and a tight junction protein, Occludin. We identified hexagalloylglucose from the extract and concluded that it is a major component regulating protection from particulate matter. Based on these results, witch hazel extract containing hexagalloylglucose is an active ingredient in anti-pollution skin care products.

Witch Hazel Significantly Improves the Efficacy of Commercially Available Teat Dips

Bovine intramammary infections (IMIs) are the main cause of economic loss in milk production. Antibiotics are often ineffective in treating infections due to antimicrobial resistance and the formation of bacterial biofilms that enhance bacterial survival and persistence. Teat dips containing germicides are recommended to prevent new IMIs and improve udder health and milk quality. IMIs are often caused by staphylococci, which are Gram-positive bacteria that become pathogenic by forming biofilms and producing toxins. As a model for a teat dip (DIP), the BacStop iodine-based teat dip (DIP) was used. Witch hazel extract (whISOBAX (WH)) was tested because it contains a high concentration of the anti-biofilm/anti-toxin phenolic compound hamamelitannin. We found that the minimal inhibitory or bactericidal concentrations of DIP against planktonic S. epidermidis cells increased up to 160-fold in the presence of WH, and that DIP was 10-fold less effective against biofilm cells. While both DIP and WH are effective in inhibiting the growth of S. aureus, only WH inhibits toxin production (tested for enterotoxin-A). Importantly, WH also significantly enhances the antibacterial effect of DIP against Gram-negative bacteria that can cause IMIs, like Escherichia coli and Pseudomonas aeruginosa. Put together, these results suggest that the antibacterial activity of DIP combined with WH is significantly higher, and thus have potential in eradicating bacterial infections, both in acute (planktonic-associated) and in chronic (biofilm-associated) conditions.

In-Vitro Inhibition of Staphylococcal Pathogenesis by Witch-Hazel and Green Tea Extracts

whISOBAX (WH), an extract of the witch-hazel plant that is native to the Northeast coast of the United States, contains significant amounts of a phenolic compound, Hamamelitannin (HAMA). Green tea (GT) is a widely consumed plant that contains various catechins. Both plants have been associated with antimicrobial effects. In this study we test the effects of these two plant extracts on the pathogenesis of staphylococci, and evaluate their effects on bacterial growth, biofilm formation, and toxin production. Our observations show that both extracts have antimicrobial effects against both strains of S. aureus and S. epidermidis tested, and that this inhibitory effect is synergistic. Also, we confirmed that this inhibitory effect does not depend on HAMA, but rather on other phenolic compounds present in WH and GT. In terms of biofilm inhibition, only WH exhibited an effect and the observed anti-biofilm effect was HAMA-depended. Finally, among the tested extracts, only WH exhibited an effect against Staphylococcal Enterotoxin A (SEA) production and this effect correlated to the HAMA present in WH. Our results suggest that GT and WH in combination can enhance the antimicrobial effects against staphylococci. However, only WH can control biofilm development and SEA production, due to the presence of HAMA. This study provides the initial rationale for the development of natural antimicrobials, to protect from staphylococcal colonization, infection, or contamination.

A seed flying like a bullet: ballistic seed dispersal in Chinese witch-hazel ( Hamamelis mollis OLIV., Hamamelidaceae)

The fruits of Chinese witch-hazel ( Hamamelis mollis , Hamamelidaceae) act as ‘drying squeeze catapults', shooting their seeds several metres away. During desiccation, the exocarp shrinks and splits open, and subsequent endocarp deformation is a complex three-dimensional shape change, including formation of dehiscence lines, opening of the apical part and formation of a constriction at the middle part. Owing to the constriction forming, mechanical pressure is increasingly applied on the seed until ejection. We describe a structural latch system consisting of connective cellular structures between endocarp and seed, which break with a distinct cracking sound upon ejection. A maximum seed velocity of 12.3 m s −1 , maximum launch acceleration of 19 853 m s −2 (approx. 2000 g ) and maximum seed rotational velocity of 25 714 min −1 were measured. We argue that miniscule morphological differences between the inner endocarp surface and seed, which features a notable ridge, are responsible for putting spin on the seed. This hypothesis is further corroborated by the observation that there is no preferential seed rotation direction among fruits. Our findings show that H. mollis has evolved similar mechanisms for stabilizing a ‘shot out’ seed as humans use for stabilizing rifle bullets and are discussed in an ecological (dispersal biology), biomechanical (seed ballistics) and functional–morphological (fine-tuning and morphospace of functional endocarps) contexts, and promising additional aspects for future studies are proposed.