Estimación de la concentración de clorofila y su relación con la concentración de proteína cruda en tres especies del pasto Urochloa en el Piedemonte Llanero, Colombia

To establish the relationship between the intensity of the green color of leaves and their crude protein (CP) concentration in Urochloa brizantha, U. decumbens and U. humidicola pastures in the Piedemonte Llanero, Colombia, the leaf chlorophyll concentration was measured (in SPAD units), classified based on the Munsell color chart and compared with the leaf CP concentration determined by the Kjeldahl method. The regression analysis between chlorophyll and CP concentrations showed coefficients of determination (r2) between 0.76 and 0.88. While collection of additional data will allow colorimetric charts based on the Munsell color chart to be developed which can be used to predict CP levels for each of these species, the relationships generated can be used to develop recommendations to assist farmers in the region in their decisions on use and fertilizing of these pastures.

KARAKTERISASI MORFOLOGI DAUN DAN BUNGA BEBERAPA VARIETAS Hoya coronaria DARI KAWASAN HUTAN KERANGAS AIR ANYIR, BANGKA

Hoya coronaria was found in Heath Forest Air Anyir, Bangka in various flower color. Characterization based on morphological characteristics need to clear up the differences between varieties. This research aims to observe morphological variation of six varieties of H. coronaria from Heath Forest Air Anyir, Bangka based on morphology vegetative and flower morphology. The descriptive method was used to describe the morphological character. Determination of color based on Munsell Color Chart for Plant Tissues. The six samples of different varieties was used which has different flower color as follow respectively: yellow corolla with pink corona (var1); yellow corolla with white corona (var2); yellow corolla with pink honey line and pink corona (var3); pink corolla with pink honey line and pink corona (var4); pink corolla with pink honey line and white corona (var5); and white corolla with white corona (var6). The six varieties have different characteristic of vegetative and flower part both on qualitative and quantitative measurement. The cluster analyses resulted two sister groups. The first group (group A) consisted of var3, var4, var5, and var6, and the second group (group B) consisted of var1 and var2. Groups A was consisted of two smaller groups, namely group C and group D. Group C consisted of var3, var4 and var5, while group D consisted of var6

Magnetic and spectral signatures of Cerrado soils in the state of Goiás, Brazil

The objective of this work was to estimate the iron oxide contents (hematite and goethite) and to characterize the color and the spectral and magnetic signatures of Cerrado soils in the state of Goiás, Brazil. Six Oxisols and one Inceptisol were studied. Spectral and magnetic signatures were determined by diffuse reflectance spectroscopy (DRS) and magnetic susceptibility, respectively. Then, the spectral curves and the second derivative calculations were used to determine hematite and goethite contents, as well as soil color after conversion into tristimulus values. Hematite and goethite contents were also obtained by x-ray diffractometry, and soil color was also defined in the field (Munsell color chart). The values for the isomorphic substitution of iron by aluminum and the degree of redness were also determined. DRS can be used to estimate hematite and goethite contents, as well as the color of Cerrado soils in the state of Goiás. The spectral signature can point out the main soil properties related to the contents of organic matter, iron oxides, kaolinite, and gibbsite. The magnetic signature, characteristic of soils rich in iron oxides (hematite and goethite), shows the predominance of pedogenic minerals.

Anthocyanin and Chlorophyll Content in Flush as Selection Markers on Fine Flavor Cocoa (Theobroma cacao L.)

Level of anthocyanin content in flush is different between fine flavor cocoa and bulk cocoa. This study was aimed to determine the potential of anthocyanin and chlorophyll content in flush as one of the selection indicator for fine flavor cocoa. Flush sampling was carried out at Cocoa Germplasm Collections in Kaliwining Experimental Station, Indonesian Coffee and Cocoa Research Institute, while anthocyanin and chlorophyll analysis was conducted at Laboratory of Ma Chung Research Center for Photosynthetic Pigments, Ma Chung University, Malang.The research utilized a completely randomized design consisted of four genotypes of fine flavor cocoa, namely DR 2, ICCRI 02, DRC 16, PNT 16 and five genotypes of bulk cocoa, namely Sulawesi 1, MCC 01, MCC 02, KW 617 and PA 191.Each treatment was repeated three times. Anthocyanin and chlorophyll content in flush was measured. Anthocyanin analysis was done by destructive methods, while flush color was observed based on Munsell Color Chart for Plant Tissues Data analysis used orthogonal contrast, multivariate and simple correlation. The results showed that there were differences in flush color on fine flavor cocoa and that of bulk cocoa. The flush of the fine flavor cocoa had a tendency towards yellow and that of bulk cocoa had a tendency toward red with different levels of variation among genotypes. The analysis results of chlorophyll content in flush showed that chlorophyll content of fine flavor cocoa was higher than bulk cocoa with a range of 2376-3692 µmol/L for fine flavor cocoa and 1567-2628 µmol/L for bulk cocoa. On the contrary, anthocyanin content in fine flavor cocoa was lower than bulk cocoa with a range of 3.82-5.72 µmol/L for fine flavor cocoa and 6.01-11.88 µmol/L for bulk cocoa. The analysis results of orthogonal contrasts showed that chlorophyll content value was more stable than anthocyanin. There was negative relationship between anthocyanin and chlorophyll in flush with the regression Y = -221,1X + 3935 with a value of correlation r = -0.50. The anthocyanin and chlorophyll content in flush had a potential to be used as selection markers for fine flavor cocoa hybrid