Impression during Coronavirus Pandemic on Behaviour of Infectious-microbial-disease and Home-physical-tools in Saudi-public

This work was for "Impression during Coronavirus Pandemic (CVP) on Behaviour of Infectious-microbial-disease (IMD) and Home-physical-tools (HPTs) in Saudi-public (SP)". The aim was for a clarify impression during CVP on changing behaviour of IMD and HPTs in SP. That was by making a questionnaire to obtain information from people in SP. It found participating 28.5% for < 20 yr and was 71.5% for > 20 yr, 31.5% were men and 68.5% were women. While 92.5% lived in families, were HPTs differed in 83.5%, 75.5% agreed presence source Coronavirus infection (CVI) in the HPTs. As well as 11.5% individuals had CVI, where 84.5% refused to use HPTs if were suspected of carrying the CVI patient. Once 94.5% was not dealing with individuals infected with CV. Anywhere, 69.5% agreed for good treatment with the CVI patient after recovery and so 73.5% acquired skills to change the behaviour of HPTs. From the whole caution handle was 29.5%, hand washing considered 20%, permanent sterilization was 18.5%. The use of gloves and facemasks was 16.5%, not use other people's tools, it took 15.5%. That concluded from this paper the approved changes behaviour of IMD and HPTs in SP, that which had important for the decrease of CVP presence in SP and worldwide. That recommended the CVP is very risk in SP and worldwide and must take care of every behaviour in house to decrease CVI. We intend to do future research in changes behaviour in house to protect family members from CVI and save SP.

The Protein Kinase A-Dependent Phosphoproteome of the Human Pathogen Aspergillus fumigatus Reveals Diverse Virulence-Associated Kinase Targets

ABSTRACTProtein kinase A (PKA) signaling plays a critical role in the growth and development of all eukaryotic microbes. However, few direct targets have been characterized in any organism. The fungus Aspergillus fumigatus is a leading infectious cause of death in immunocompromised patients, but the specific molecular mechanisms responsible for its pathogenesis are poorly understood. We used this important pathogen as a platform for a comprehensive and multifaceted interrogation of both the PKA-dependent whole proteome and phosphoproteome in order to elucidate the mechanisms through which PKA signaling regulates invasive microbial disease. Employing advanced quantitative whole-proteomic and phosphoproteomic approaches with two complementary phosphopeptide enrichment strategies, coupled to an independent PKA interactome analysis, we defined distinct PKA-regulated pathways and identified novel direct PKA targets contributing to pathogenesis. We discovered three previously uncharacterized virulence-associated PKA effectors, including an autophagy-related protein, Atg24; a CCAAT-binding transcriptional regulator, HapB; and a CCR4-NOT complex-associated ubiquitin ligase, Not4. Targeted mutagenesis, combined with in vitro kinase assays, multiple murine infection models, structural modeling, and molecular dynamics simulations, was employed to characterize the roles of these new PKA targets in growth, environmental and antimicrobial stress responses, and pathogenesis in a mammalian system. We also elucidated the molecular mechanisms of PKA regulation for these effectors by defining the functionality of phosphorylation at specific PKA target sites. We have comprehensively characterized the PKA-dependent phosphoproteome and validated PKA targets as direct regulators of infectious disease for the first time in any pathogen, providing new insights into PKA signaling and control over microbial pathogenesis.IMPORTANCE PKA is essential for the virulence of eukaryotic human pathogens. Understanding PKA signaling mechanisms is therefore fundamental to deciphering pathogenesis and developing novel therapies. Despite its ubiquitous necessity, specific PKA effectors underlying microbial disease remain unknown. To address this fundamental knowledge gap, we examined the whole-proteomic and phosphoproteomic impacts of PKA on the deadly fungal pathogen Aspergillus fumigatus to uncover novel PKA targets controlling growth and virulence. We also defined the functional consequences of specific posttranslational modifications of these target proteins to characterize the molecular mechanisms of pathogenic effector regulation by PKA. This study constitutes the most comprehensive analysis of the PKA-dependent phosphoproteome of any human pathogen and proposes new and complex roles played by PKA signaling networks in governing infectious disease.

Modern antimicrobial therapy in dental caries

This article reviews the current therapeutic attitude on dental caries prevention involving antimicrobial strategies, based on the modern definition of dental caries as a microbial disease. The most important and used prophylactic measures are: topical and systemic administration of fluorinated products, diet control and maintenance of a rigorous dental oral hygiene.