The year public health lost its soul

The COVID pandemic was an acute test of public health capacities worldwide. Many will hail the successes obtained and stress the importance of the discipline. On the contrary, this commentary defends the idea that the COVID pandemic response forced public health to enter in a Faustian bargain with governments and realpolitik that threaten the very core of the discipline’s principles.

Comparison of Joint Effect of Acute and Chronic Toxicity for Combined Assessment of Heavy Metals on Photobacterium sp.NAA-MIE

Predicting the crucial effect of single metal pollutants against the aquatic ecosystem has been highly debatable for decades. However, dealing with complex metal mixtures management in toxicological studies creates a challenge, as heavy metals may evoke greater toxicity on interactions with other constituents rather than individually low acting concentrations. Moreover, the toxicity mechanisms are different between short term and long term exposure of the metal toxicant. In this study, acute and chronic toxicity based on luminescence inhibition assay using newly isolated Photobacterium sp.NAA-MIE as the indicator are presented. Photobacterium sp.NAA-MIE was exposed to the mixture at a predetermined ratio of 1:1. TU (Toxicity Unit) and MTI (Mixture Toxic Index) approach presented the mixture toxicity of Hg2+ + Ag+, Hg2+ + Cu2+, Ag+ + Cu2+, Hg2+ + Ag+ + Cu2+, and Cd2+ + Cu2+ showed antagonistic effect over acute and chronic test. Binary mixture of Cu2+ + Zn2+ was observed to show additive effect at acute test and antagonistic effect at chronic test while mixture of Ni2+ + Zn2+ showing antagonistic effect during acute test and synergistic effect during chronic test. Thus, the strain is suitable and their use as bioassay to predict the risk assessment of heavy metal under acute toxicity without abandoning the advantage of chronic toxicity extrapolation.

The year public health lost its soul

The COVID pandemic was an acute test of public health capacities worldwide. Many will hail the successes obtained and stress the importance of the discipline. On the contrary, this commentary defends the idea that the COVID pandemic response forced public health to enter in a Faustian bargain with governments and realpolitik that threaten the very core of the discipline’s principles.

The year public health lost its soul

The COVID pandemic was an acute test of public health capacities worldwide. Many will hail the successes obtained and stress the importance of the discipline. On the contrary, this commentary defends the idea that the COVID pandemic response forced public health to enter in a Faustian bargain with governments and realpolitik that threaten the very core of the discipline’s principles.

Research article Toxicity Studies on Kushta Khabasul Hadeed (calcined iron rust) prepared by Classical and Muffle Furnace Methods

Background: Though a number of studies has been carried out in terms of safety of Kushta but concerns have been raised about the presence of heavy metals in Unani formulations. Classical method of preparation of KKH and other Kushta preparation in Unani system of medicine has been found to eliminate all such issues from the end product. Hence; the present study was designed to evaluate the acute and sub-acute toxicity study of KKH prepared by Classical and Muffle furnace methods. Objective: To ascertain the safety profile of Kushta Khabasul Hadeed (KKH) prepared by classical and muffle furnace methods. Materials and methods: Both of the samples of KKH i.e. Kushta Khabasul Hadeed (KKHCM) and Kushta Khabasul Hadeed (KKHMFM) prepared by Classical and Muffle Furnace Methods respectively were evaluated for acute and sub-acute toxicity by OECD guidelines 423 and 407 respectively. The starting dose in the acute test was 50 mg/kg and in sub-acute test, it was 134mg, 200 mg and 400 mg/kg b.w. Effect of both of the test samples were assessed for ponderal changes, food and water intake, relative organs weights, hematological, biochemical and histopathological features of various organs. Results: Both of the samples did not produce any sign of toxicity in acute toxicity study up to the dose level of 2000mg/kg b.w. In sub-acute toxicity study no mortality in any of the test groups was observed. KKHCM at different doses showed a slight decrease in food intake in all the test groups but significant difference (P<0.05) was observed in the test group B as compared to plain control. Conclusion: KKHMFM was found to be relatively toxic than KKHCM at different dose levels. The study demonstrated that both the Classical and Muffle furnace methods of preparation of Kushta are useful for preparing KKH and also safe at the dose prescribed in Unani literature. However, the Kushta prepared by the classical method was found to be safer than the muffle furnace method.

Comparative sensitivities of larval stages of the cane toad, Rhinella marina, and the striped marsh frog, Limnodynastes peronii, to atrazine

Variations in larval sensitivities to atrazine were determined in the Australian native striped marsh frog, Limnodynastes peronii, and the introduced cane toad, Rhinella marina. The static acute test design involved six nominal concentrations of atrazine, including control, solvent control, 3, 6, 12, and 24 mg L–1. Gosner stages 22–23 as hatchlings, stages 25–26, 28–29, and 32–33 as premetamorphic, 36–37 as prometamorphic and 40–41 as metamorphic climax stages of cane toads and the first four sets of Gosner stages of striped marsh frogs were exposed to atrazine treatments for 96 h. Results showed that late larval stages were more sensitive than early stages and different premetamorphic stages showed variations in sensitivities in both test species. The striped marsh frog showed a stronger concentration- and stage-dependent response and greater sensitivity to atrazine than the cane toad. In both experimental species, Gosner stages 28–29 showed better concentration-dependent increase in sensitivities to atrazine compared with other larval stages. It can be concluded that inter- and intra-species variations in sensitivities to atrazine may occur in Australian anurans and native species may show greater sensitivity to acute concentrations of atrazine than the introduced cane toad.

Assessment of sediment quality and pore water ecotoxicity in Kebir Rhumel basin (NE-Algeria): a combined approach

The objectives of this study are to use different approaches to assess the current pollution status in the wadis of the Kebir Rhumel basin. First, sediment trace metal contents were measured by flame atomic absorption spectroscopy. Then, sediment quality was assessed on the basis of contamination assessment indexes such as: Geoaccumulation Index (Igeo), Contamination factor (Cf), Contamination degree (Cd), Sediment Pollution Index (SPI) and SEQ guidelines (Consensus Sediment Quality Guidelines). In addition, several toxicity tests (Daphnia magna mobility inhibition acute test-48 h, Aliivibrio fischeri luminescence inhibition acute test – 15/30 mn and Pseudokirchneriella subcapitata growth inhibition chronic test – 72 h) were conducted to assess sediment pore water ecotoxicity. Trace metal concentrations followed the order: Mn &gt; Zn &gt; Pb &gt; Cr &gt; Cu &gt; Ni &gt; Co &gt; Cd. Indexes used indicate varying degrees of sediment quality. Igeo, Cf, Cd and SPI reveal a polymetallic contamination dominated by two or more elements in which Cd, Cu and Pb are of greatest concern. SEQ guidelines showed that biological effects on fauna would likely be observed occasionally and/or frequently for Cd, Cr, Cu, Pb and Zn contents. Test organisms exposed to sediment pore water showed that the algal P. subcapitata test was more sensitive than the D. magna and A. fischeri tests. Hence, algal growth inhibition proved to be the most sensitive response to contaminants present in sediment extracts but a significant relationship with trace metal contents was not demonstrated.