Monitoring fish communities through environmental DNA metabarcoding in the fish pass system of the second largest hydropower plant in the world

The Itaipu Hydroelectric Power Plant is the second largest in the world in power generation. The artificial barrier created by its dam imposes an obstacle for fish migration. Thus, in 2002, a fish pass system, named Piracema Channel, was built to allow fish to access areas upstream of the reservoir. We tested the potential of environmental DNA metabarcoding to monitor the impact of both the dam and associated fish pass system in the Paraná River fish communities and to compare it with traditional monitoring methods. Using a fragment of the 12S gene, we characterized richness and community composition based on amplicon sequence variants, operational taxonomic units, and zero-radius OTUs. We combined GenBank and in-house data for taxonomic assignment. We found that different bioinformatics approaches showed similar results. Also, we found a decrease in fish diversity from 2019 to 2020 probably due to the recent extreme drought experienced in southeastern Brazil. The highest alpha diversity was recorded in the mouth of the fish pass system, located in a protected valley with the highest environmental heterogeneity. Despite the clear indication that the reference databases need to be continuously improved, our results demonstrate the analytical efficiency of the metabarcoding to monitor fish species.

Monitoring fish communities through DNA metabarcoding in the fish pass system of the second largest hydropower plant in the world

The Itaipu Hydroelectric Power Plant is the second largest in the world in power generation. The artificial barrier created by its dam imposes an obstacle for fish migration. Thus, in 2002, a fish pass system, named Piracema Channel, was built to allow fish to access areas upstream of the reservoir. We tested the potential of metabarcoding to monitor the impact of both the dam and associated fish pass system in the Parana River fish communities and to compare it with traditional monitoring methods. Using a fragment of the 12S gene, we characterized richness and community composition based on amplicon sequence variants, operational taxonomic units, and zero-radius OTUs. We combined GenBank and in-house data for taxonomic assignment. We found that different bioinformatics approaches showed similar results. Also, we found a decrease in fish diversity from 2019 to 2020 probably due to the recent extreme drought experienced in southeastern Brazil. The highest alpha diversity was recorded in the mouth of the fish pass system, located in a protected valley with the highest environmental heterogeneity. Despite the clear indication that the reference databases need to be continuously improved, our results demonstrate the analytical efficiency of the metabarcoding to monitor fish species.

Modified dynamic boundary conditions (mDBC) for general-purpose smoothed particle hydrodynamics (SPH): application to tank sloshing, dam break and fish pass problems

Dynamic boundary conditions (DBC) for solid surfaces are standard in the weakly compressible smoothed particle hydrodynamics (SPH) code DualSPHysics. A stationary solid is simply represented by fixed particles with pressure from the equation of state. Boundaries are easy to set up and computations are relatively stable and efficient, providing robust numerical simulation for complex geometries. However, a small unphysical gap between the fluid and solid boundaries can form, decreasing the accuracy of pressures measured on the boundary. A method is presented where the density of solid particles is obtained from ghost positions within the fluid domain by linear extrapolation. With this approach, the gap between fluid and boundary is reduced and pressures in still water converge to hydrostatic, including the case of a bed with a sharp corner. The violent free-surface cases of a sloshing tank and dam break impact on an obstacle show pressures measured directly on solid surfaces in close agreement with experiments. The complex 3-D flow in a fish pass, with baffles to divert the flow, is simulated showing close agreement with measured water levels with weirs open and gates closed, but less close with gates open and weirs closed. This indicates the method is suitable for rapidly varying free-surface flows, but development for complex turbulent flows is necessary. The code with the modified dynamic boundary condition (mDBC) is available in DualSPHysics to run on CPUs or GPUs.

Design and Function Monitoring of an Enature® Vertical Slot Fish Pass in a Large Potamal River in Carinthia/Austria

To establish good ecological status in European rivers, firstly there is the question of how to re-establish unhindered migration for fish at barriers. This article documents a project to re-establish longitudinal connectivity at a large epipotamal river at the Schwabeck Hydro Power Plant, Carinthia/Austria, from the selection of an appropriate fish pass system to the final function control. Instead of a standard vertical slot, the innovative enature® fish pass shape with a significant reduction of flow, velocities, energy dissipation rate, and turbulences, but with a clear enhancement of fish passage capability, was chosen. Using 2D hydraulic modelling and a statistical evaluation of fish passage, physical and ecological effects were reviewed, with the clear result that there is no identifiable, positive ecological effect on the number of fish migrating with an increase of concurrent flow in the fish pass. Passability and findability were monitored with the new FishCam, an automatic, precise, and constant (24/7 24 h a day, seven days a week) collection and pre-evaluation field data survey method which does not involve trapping of, contact with, or stress for fish. It was shown that the enature® fish pass enables an unhindered migration for all available fish species. As >99% of fish migrate from April to November, there is no ecological need to operate a fully functional fish pass year-round. Combining all the individual factors together, the fish pass at the Schwabeck Hydro Power Plant is an almost exemplary solution for a fully functioning restauration of the continuum with a minimized loss of generation of electricity.

Fish species using the fish passage between Jamuna and Bangali river at Sariakandi, Bogra

Sariakandi fish pass is unique connecting Jamuna and Bangali rivers through an engineered channel. A study was conducted on the fish pass aiming to improve fish diversity in north-west Bangladesh. Sampling at the fish pass, market census, interviewing the locals and focus group discussion were done to collect data from the study area. A total of 69 fish species under 9 orders and 26 families were recorded. Cypriniformes (22 species) was the most dominant order followed by Siluriformes (21 species), Perciformes (13 species), Clupeiformes (4 species), Synbranchiformes (4 species), Osteoglossiformes (2 species), Mugiliformes (1 species), Anguilliformes (1 species) and Tetraodontiformes (1 species). In total 30 threatened species in Bangladesh and globally recognized 9 species were recorded. This fish pass is not working during the dry or winter seasons but helps in the diversified fish migration diversity of Bengali and Jamuna river system during the flood time of Bangladesh. The present study recommends establishing effective fish passages in flood protection structures or dams in Bangladesh to retain the connectivity of fish migration routes and the improving of fish diversity. J. Biodivers. Conserv. Bioresour. Manag. 2019, 5(2): 53-62