Impact of loss‐of‐function of FATTY ACID DESATURASE 7 in Arabidopsis thaliana on the green peach aphid, Myzus persicae , and the cabbage aphid, Brevicoryne brassicae

Fatty acid desaturases (FADs) in plants influence levels of susceptibility to multiple stresses, including insect infestations. In this study, infestations of the greAtFABen peach aphid (Myzus persicae) on Arabidopsis thaliana were reduced by mutations in three desaturases: FAB2/SSI2, which encodes a chloroplastic stearoyl-[acyl-carrier-protein] 9-desaturase, and AtFAD7 or AtFAD3, which encode ω-3 FADs in the chloroplast and endoplasmic reticulum (ER) respectively. These data indicate that certain FADs promote susceptibility to aphids, and that aphids are impacted by desaturases in both the chloroplast and ER. Aphid resistance in ssi2, fad3, and/or fad7 might involve altered signaling between these subcellular compartments. C18:1 levels are depleted in ssi2, whereas C18:2 accumulation is enhanced in fad3 and fad7. In contrast, fad8 has higher than normal C18:2 levels but also high C18:1 and low C18:0, and does not impact aphid numbers. Potentially, aphids may be influenced by the balance of multiple fatty acids (FAs) rather than by a single species, with C18:2 promoting aphid resistance and C18:1 promoting susceptibility. Although the fad7 mutant also accumulates higher-than-normal levels of C16:2, this FA does not contribute to aphid resistance because a triple mutant line that lacks detectable levels of C16:2 (fad2fad6fad7) retains comparable levels of aphid resistance as fad7. In addition, aphid numbers are unaffected by the fad5 mutation that inhibits C16:1 synthesis. Together, these results demonstrate that certain FADs are important susceptibility factors in plant-aphid interactions, and that aphid resistance is more strongly associated with differences in C18 abundance than C16 abundance.

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POPULATION DYNAMICS OF APHIDS ON BROCCOLI IN THE MEXICAN BAJIO VALLEY.

HortScience ◽

10.21273/hortsci.25.9.1132a ◽

1990 ◽

Vol 25 (9) ◽

pp. 1132a-1132

Author(s):

Alfonso Hernández-Barajas ◽

José Luis Vera-Medina ◽

Dr. Benito Alvarado-Rodriguez

Keyword(s):

Population Dynamics ◽

Myzus Persicae ◽

Green Peach Aphid ◽

Parasitic Wasp ◽

Brevicoryne Brassicae ◽

Aphid Population ◽

Cabbage Aphid ◽

Prevalent Species ◽

Natural Parasitism ◽

Made In

During a two-year-period, a study was made in the field in order to know the ocurrence of aphids in the broccoli crop. It was possible to know that the total aphid population is higher in winter plantings where more than 240 aphids/plant were found Spring and fall plantings, in comparison, less than 20 aphids/plant were encountered. The species more common were the cabbage aphid (Brevicoryne brassicae L.), the most prevalent species in the crop, getting more than 80% of the population, and the green peach aphid (Myzus persicae Sulzer) with a little bit more than 17% of the total. The natural parasitism in all plantings was around 17%. By species, parasitism in B. brassicae was higher in springs (70% of the total) and fall plantings where the parasitic wasp Diaretiella rapa e (MacIntosh) was probably more active. The sane pattern was obtained for M. persicae, in which the parasitism was again higher in spring and fall plantings (20% of the total) and very low during the winter. Regarding leaf preference, a very defined pattern was found in the M. persicae than for B. brassicae.

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THE ROLE OF THE APHIDS, BREVICORYNE BRASSICAE AND MYZUS PERSICAE, IN THE SPREAD OF XANTHOMONAS CAMPESTRIS, THE CAUSAL ORGANISM OF BLACK ROT OF TURNIPS

Canadian Journal of Research ◽

10.1139/cjr50c-043 ◽

1950 ◽

Vol 28c (6) ◽

pp. 699-705 ◽

Cited By ~ 1

Author(s):

Norma M. Stapleton ◽

L. A. McDermott

Keyword(s):

Myzus Persicae ◽

Xanthomonas Campestris ◽

Green Peach Aphid ◽

Virulent Strain ◽

Brevicoryne Brassicae ◽

Black Rot ◽

Causal Organism ◽

Cabbage Aphid ◽

Petri Plate ◽

Rot Disease

Turnip plants, heavily infested with the cabbage aphid, Brevicoryne brassicae (L.), and the green peach aphid, Myzus persicae (Sulz.), were inoculated with a virulent strain of Xanthomonas campestris (Pammel), the causal organism of black rot disease of turnips, and placed in special cages together with healthy turnip plants. Some aphids of both species were allowed to migrate naturally, and others were transferred mechanically from the diseased to the healthy plants, but no symptoms of black rot ever appeared in any of the latter plants. X. campestris was isolated from only 15% of the B. brassicae and from less than 1% of the M. persicae taken from the diseased plants. Aphids of both species were artificially contaminated externally with X. campestris by exposures to Petri plate and broth cultures of the pathogen. Internal contamination of other aphids of both species was accomplished by feeding them 0.2% aqueous dextrose solutions containing X. campestris. The pathogen was isolated from individuals m both groups of aphids thus contaminated, but none of the aphids in either group was able to infect caged, healthy turnip plants with black rot.

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Faculty Opinions recommendation of The Arabidopsis thaliana dihydroxyacetone phosphate reductase gene SUPPRESSSOR OF FATTY ACID DESATURASE DEFICIENCY1 is required for glycerolipid metabolism and for the activation of systemic acquired resistance.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1017793.207435 ◽

2004 ◽

Author(s):

Sjef Smeekens

Keyword(s):

Arabidopsis Thaliana ◽

Fatty Acid ◽

Systemic Acquired Resistance ◽

Acquired Resistance ◽

Fatty Acid Desaturase ◽

Dihydroxyacetone Phosphate ◽

Reductase Gene

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Loss of Function of Fatty Acid Desaturase 7 in Tomato Enhances Photosynthetic Carbon Fixation Efficiency

Frontiers in Plant Science ◽

10.3389/fpls.2020.00932 ◽

2020 ◽

Vol 11 ◽

Author(s):

Janithri S. Wickramanayake ◽

Josue A. Goss ◽

Min Zou ◽

Fiona L. Goggin

Keyword(s):

Fatty Acid ◽

Carbon Fixation ◽

Fatty Acid Desaturase ◽

Loss Of Function ◽

Photosynthetic Carbon Fixation ◽

Photosynthetic Carbon

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Susceptibility of Myzus persicae, Brevicoryne brassicae and Nasonovia ribisnigri to Fungal Biopesticides in Laboratory and Field Experiments

Insects ◽

10.3390/insects11010055 ◽

2020 ◽

Vol 11 (1) ◽

pp. 55

Author(s):

Gill Prince ◽

Dave Chandler

Keyword(s):

Field Experiment ◽

Myzus Persicae ◽

Field Experiments ◽

Brevicoryne Brassicae ◽

Vegetable Crops ◽

Potato Aphid ◽

Cabbage Aphid ◽

Nasonovia Ribisnigri ◽

Fungal Biopesticides ◽

Peach Potato Aphid

The aim of this study was to evaluate the potential of entomopathogenic fungi (EPF) for the control of aphid pests of field vegetable crops. Four biopesticides based on the EPF Beauveria bassiana (Botanigard ES and Naturalis L), Cordyceps fumosorosea s.l. (Preferal WG), and Akanthomyces dipterigenus (Vertalec) were evaluated in a laboratory bioassay against peach-potato aphid Myzus persicae, cabbage aphid Brevicoryne brassicae, and currant-lettuce aphid Nasonovia ribisnigri. There was significant variation in the spore dose provided by the products, with Botanigard ES producing the highest dose (639 viable spores per mm2). Botanigard ES also caused more mortality than the other products. Combining Vertalec with the vegetable oil-based adjuvant Addit had an additive effect on the mortality of B. brassicae. All fungal products reduced the number of progeny produced by M. persicae but there was no effect with B. brassicae or N. ribisnigri. When aphid nymphs were treated with Botanigard ES and Preferal WG, both products reduced population development, with up to 86% reduction occurring for Botanigard ES against M. persicae. In a field experiment, Botanigard ES sprayed twice, at seven-day intervals, against B. brassicae on cabbage plants, reduced aphid numbers by 73%. In a second field experiment with B. brassicae, M. persicae, and N. ribisnigri, Botanigard ES reduced populations of B. brassicae and N. ribisnigri but there was no significant effect on M. persicae.

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