Modeling Bacterial Attachment Mechanisms on Superhydrophobic and Superhydrophilic Substrates

Superhydrophilic and superhydrophobic substrates are widely known to inhibit the attachment of a variety of motile and/or nonmotile bacteria. However, the thermodynamics of attachment are complex. Surface energy measurements alone do not address the complexities of colloidal (i.e., bacterial) dispersions but do affirm that polar (acid-base) interactions (ΔGAB) are often more significant than nonpolar (Lifshitz-van der Waals) interactions (ΔGLW). Classical DLVO theory alone also fails to address all colloidal interactions present in bacterial dispersions such as ΔGAB and Born repulsion (ΔGBorn) yet accounts for the significant electrostatic double layer repulsion (ΔGEL). We purpose to model both motile (e.g., P. aeruginosa and E. coli) and nonmotile (e.g., S. aureus and S. epidermidis) bacterial attachment to both superhydrophilic and superhydrophobic substrates via surface energies and extended DLVO theory corrected for bacterial geometries. We used extended DLVO theory and surface energy analyses to characterize the following Gibbs interaction energies for the bacteria with superhydrophobic and superhydrophilic substrates: ΔGLW, ΔGAB, ΔGEL, and ΔGBorn. The combination of the aforementioned interactions yields the total Gibbs interaction energy (ΔGtot) of each bacterium with each substrate. Analysis of the interaction energies with respect to the distance of approach yielded an equilibrium distance (deq) that seems to be independent of both bacterial species and substrate. Utilizing both deq and Gibbs interaction energies, substrates could be designed to inhibit bacterial attachment.

Anisotropic Colloidal Interactions & Assembly in AC Electric Fields

We match experimental and simulated configurations of anisotropic epoxy colloidal particles in high frequency AC electric fields by identifying analytical potentials for dipole-field and dipole-dipole interactions. We report an inverse...

Interaction Patterns for Staggered Assembly of Fibrils from Semiflexible Chains

The design of colloidal interactions to achieve target self-assembled structures has especially been done for compact objects such as spheres with isotropic interaction potentials, patchy spheres and other compact objects with patchy interactions. Inspired by the self-assembly of collagen-I fibrils and intermediate filaments, we here consider the design of interaction patterns on semiflexible chains that could drive their staggered assembly into regular (para)crystalline fibrils. We consider semiflexible chains composed of a finite number of types of interaction beads (uncharged hydrophilic, hydrophobic, positively charged and negatively charged) and optimize the sequence of these interaction beads with respect to the interaction energy of the semiflexible chains in a number of target-staggered crystalline packings. We find that structures with the lowest interaction energies, that form simple lattices, also have low values of L/D (where L is chain length and D is stagger). In the low interaction energy sequences, similar types of interaction beads cluster together to form stretches. Langevin Dynamics simulations confirm that semiflexible chains with optimal sequences self-assemble into the designed staggered (para)crystalline fibrils. We conclude that very simple interaction patterns should suffice to drive the assembly of long semiflexible chains into staggered (para)crystalline fibrils.