VAGINAL FIBROSARCOMA IN GOLDEN RETRIEVER DOG: CASE REPORT

A Golden Retriever bitch, 10 years old, came to the Animal Hospital of Brawijaya University, Malang, Indonesia, her conditions was discharge from the vagina, stink smell, polyuria, polydipsia and swelling in the vaginal area that begin to appear one month ago. Physical examination results found her weight 30 kg, scored three for body condition score (scale 1-5), body temperature at 38ºC, pale mucous membrane, and Capillart Reill Time > 2 seconds. Hard mass was palpable in the vaginal area. Based on the anamnesis, physical examination and clinical symptoms, the bitch appeared have some different diagnosis such as pyometra, endometritis, vaginitis, and vaginal tumors. Hematology, radiograph, and histopathological laboratory tests were performed to confirm the diagnosis. Hematologic results showed the bitch suffered from leukocytosis of 41.7 x 103/?L, granulocytosis 36.5 x 103/?L, and hyperchromic microcytic anemia. The results of the radiograph examination showed a radiopaque solid mass in the vagina and uterus. The results of the histopathological examination found there were hyperchromasia nuclei and pleomorphism of fibroblast cell, and formation spindles cell of connective tissue. Based on the results of physical examination, hematological test, radiographic test, and histopathology, the dog was diagnosed with fibrosarcoma in the vagina. The treatment of this case was ovariohysterectomy and removal of the vaginal tumor. Early diagnosis and early treatment largely determine the patient’s prognosis, metastatic vaginal fibrosarcoma tumors showed poor prognosis.

Haemoparasites infection in bullfighting cattle in southern of Thailand

Haemoparasites affect animal health and the production leading to economic losses and may have effects on human health. In Thailand, there are few reports of infections in the bullfighting cattle are for cultural fighting competitions. Therefore, this research aimed to investigate the distribution of haemoparasites infection in bullfighting cattle in order to control the transmission of disease to the local domestic cattle. A total of 1906 blood samples were collected from the bullfighting cattle from eight provinces in southern Thailand from 2017 to 2020. Those cattle showed the clinical signs of gasping, pale mucous membrane, haematuria, anorexia, sneezing and weakness. Micro-haematocrit centrifugation and thin blood smears with Giemsa’s staining were used. The results indicated 42.50% (810/1906) of cases positive for haemoparasites infection. The most commonly found was Theileria spp. (38.20%, 728/1906) followed by microfilaria of Setaria spp. (2.68%, 51/1906), Trypanosoma spp. (1.21%, 23/1906), Babesia spp. (0.94%, 18/1906) and Anaplasma spp. (0.10%, 2/1906). Out of 810 positive samples, there was co-infection between Theileria spp. and Setaria spp. (1.36%, 11/810), Theileria spp. and Babesia spp. (0.99%, 8/810), and Theileria spp., Trypanosoma spp. and Setaria spp. (0.37%, 3/810). The distribution of haemoparasites infection in eight raising areas ranged from 39.71 to 75%, with no significantly differences. From this result is was determined that haemoparasites were of generalised distribution in southern Thailand. Therefore, there should be strict surveillance and control of movement of cattle into the fighting arena. Moreover, vector control and blood checking before fighting should be promoted.

Clinical and Ultrasonographic Alterations in Canine Babesiosis

Canine babesiosis is a worldwide tick borne disease. Dogs with fever, in appetence and enlarged lymph nodes were screened for the presence of haemoprotozoan parasites during the one year period of study at T.V.C.C., C.V.Sc., Mhow (M.P. , India ). Based on the stained peripheral blood smears examination, dogs were found to be affected with babesiosis. Clinical examination of the dogs revealed ticks over the body, highest frequency (75.00%) of petechial/epistaxis followed by ticks (72.22%) on the body, recumbency (69.44%), dullness (66.66%), dehydration (63.88%), pale mucous membrane (50.00%) . Sonographic changes in dogs infected with babesiosis revealed hepatomegaly with architectural changes, hypo-echoic changes in the liver parenchyma in 7 dogs, splenomegaly with disturbed architecture and multiple lesions was observed in 9 dogs, cystitis in 5 dogs , distended gall bladder in 3 dogs and in 4 dogs Kidneys structures were not clear in image and no demarcation noticed between cortex and medulla.

Diagnosis and Therapeutic Management of Theileriosis in Cattle Calves

The present investigation was carried out among ten cross-bred Cattle calves of age between 1-5 months which were presented to Veterinary Clinical Complex of College of Veterinary and Animal Science, Bikaner with the history of anorexia, high fever, diarrhoea and tick infestation. Upon clinical examination pale mucous membrane, enlarged prescapular lymph nodes and increase in respiration rate and pulse rate were found. Blood samples were collected from jugular vein in vacutainers containing EDTA for haemogram. Microscopic examination of Giemsa stained thin blood smears revealed piroplasms in erythrocytes and lymph node aspirate smears revealed presence of schizonts (Koch’s blue bodies) in lymphocytes. Upon haematological examination it revealed that the decrease in haemoglobin, total erythrocyte count, packed cell volume and lymphocytes, however increase in total leukocyte count and neutrophils. After confirmation of Theileriosis in calves Buparvaquone was administered at the dose rate of 1ml/20kg body weight via intramuscular route. Eight calves were very well responded to the treatment but two calves were eventually died due to severe anaemia.

Therapeutic management of blood transfusion reaction in a crossbred cow

A crossbred cow aged 6 year was presented to Teaching Veterinary Hospital of the Institute with the history of fever, tick infestation, anemia and decreased milk yield since last 10 days. Clinical examination revealed fever, pale mucous membrane and nil rumen motility. Hematological examination findings were anemia, leucopenia, relative neutrophilia and lymphopenia and decreased platelets. Blood smear examination revealed Anaplasma Marginale infection. Animal was treated with blood transfusion, oxytetracyclin, oral hematinic and multivitamin. After half an hour of start of transfusion, cow showed transfusion reaction in the form of urticarial eruptions all over the body, edema of eyelids, lips and over the face, frothy discharge from mouth with muscle tremor and shivering. Transfusion reaction was successfully managed with Adrenalin, Dexona, Avil along with normal saline.

Clinical, Hematologic, and Molecular Findings in Naturally OccurringBabesia canis vogeliin Egyptian Dogs

Background. Canine babesiosis is a clinically important hemoprotozoan parasite affecting dogs. The goal of this present study was to determine the clinical symptoms and to establish its hematological and microscopic detection and compare it with the PCR findings attained from dogs infected withBabesia canis vogeli.Methodology/Principal Findings. 13-PCR confirmed Babesia-infected dogs were examined; seminested PCR was used to discover the precise type ofBabesiaandBabesia canis vogeliwas the only subspecies detected. The most consistent clinical signs were elevated rectal temperature and a pale mucous membrane. Thrombocytopenia, monocytosis, and lymphocytosis, along with a significant reduction in red cell parameters, were the most commonly recorded hematologic alterations. Microscopic examination revealed the presence of typical large merozoites and trophozoites ofB. canisin the ratio 76.92%.Conclusions/Significance. The presumptive diagnosis of canine babesiosis should be based on a fever and anemia, while thrombocytopenia is considered the hallmark of the disease; microscopic examination may not be very revealing in the detection at low parasitemia, but it remains the most rapid confirmatory method. Seminested PCR turned out to be a sensitive and accurate method for diagnosis; during the process of differentiation betweenBabesiasubspecies, onlyB. canis subsp. vogeliwas detected.

INCIDENCE OF ASCARIASIS IN CALVES REARED UNDER RURAL CONDITION

Faecal examination of 189 randomly selected rural calves in 12 villages of Rajoir upazila under Madaripur district of both sexes aged between < 3 months to 1 year old with or without the signs of gastroenteritis during three months periods from June to August 2003 revealed that 64.5 % calves were infected with Toxocara vitulorum. Significantly (p < 0.01) higher incidence of ascariasis was found in cross bred calves (64.28% - 75.67%) than in indigenous calves (46.66%). Age-wise analysis indicated that ascariasis was significantly (p < 0.01) prevalent in calves upto 3 months (81.35%) of age following by 3 to 6 months (70.58%) in comparison to > 6 to 12 months (41.93%) of age. Incidence of T. vitulorum infection was significantly (p < 0.01) higher in male calves (73.24%) than in female (57.92%). All the calves infected with ascariasis were diarrhoeic (100%), with pale mucous membrane (71.31%), rough body coat (74.59%) and emaciation (65.57%). Cachexia (19.67%) and erected hair (4.91%) were also recorded as clinical signs. Normal temperature was found in 95.08% calves while subnormal temperature recorded in 4.91% calves infected with T. vitulorum.