unconditional stimulus
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2021 ◽  
Vol 16 (10) ◽  
pp. 163-173
Author(s):  
D. S. Donskaya

The increasing complexity and digitalization of cross-border relations are an unconditional stimulus for the development and popularization of online dispute resolution mechanisms. The paper examines the European Union experience in regulating modern online mechanisms for resolving consumer disputes and attempts to determine the hierarchy of regulatory acts in relation to online resolution (settlement) of cross-border private law disputes. The author analyzes the features of the European approach to regulating the system of online resolution of crossborder disputes in order to determine the possible vector of development of national legislation as a condition necessary for building and ensuring the functioning of an effective system. The paper notes the advantages and disadvantages of the European system of online settlement of cross-border disputes with the participation of consumers. It concludes that it is necessary to resolve the issue of the applicability of the norms of existing treaties, adapt national legislation to the specifics of online dispute resolution, as well as ensure the possibility of considering cross-border disputes with consumer participation in accordance with standardized rules in order to simplify the dispute resolution procedure and build trust in the online system.


PLoS ONE ◽  
2021 ◽  
Vol 16 (7) ◽  
pp. e0254592
Author(s):  
Caroline Novara ◽  
Cindy Lebrun ◽  
Alexandra Macgregor ◽  
Bruno Vivet ◽  
Pierre Thérouanne ◽  
...  

Background Obsessive-compulsive disorder (OCD) has long been considered as an anxiety disorder, disgust is the dominant emotion in contamination-based OCD. However, disgust seems resistant to exposure with response prevention partly due to the fact that disgust is acquired through evaluative conditioning. Aims The present research investigates a counter-conditioning intervention in treating disgust-related emotional responses in two groups of individuals with high (High contamination concerns, HCC, n = 24) and low (Low contamination concerns LCC, n = 23) contamination concerns. Methods The two groups completed a differential associative learning task in which neutral images were followed by disgusting images (conditioned stimulus; CS+), or not (CS-). Following this acquisition phase, there was a counter-conditioning procedure in which CS+ was followed by a very pleasant unconditional stimulus while CS- remained unreinforced. Results Following counter-conditioning, both groups reported significant reduction in their expectancy of US occurrence and reported less disgust with CS+. For both expectancy and disgust, reduction was lower in the HCC group than in the LCC group. Disgust sensitivity was highly correlated with both acquisition and maintenance of the response acquired, while US expectation was predicted by anxiety. Conclusion Counter-conditioning procedure reduces both expectations and conditioned disgust.


2021 ◽  
Vol 15 ◽  
Author(s):  
Henk-Jan Boele ◽  
Sangyun Joung ◽  
Joanne E. Fil ◽  
Austin T. Mudd ◽  
Stephen A. Fleming ◽  
...  

Introduction: Pigs have been an increasingly popular preclinical model in nutritional neuroscience, as their anatomy, physiology, and nutrition requirements are highly comparable to those of humans. Eyeblink conditioning is one of the most well-validated behavioral paradigms in neuroscience to study underlying mechanisms of learning and memory formation in the cerebellum. Eyeblink conditioning has been performed in many species but has never been done on young pigs. Therefore, our aim here was to develop and validate an eyeblink conditioning paradigm in young pigs.Method: Eighteen intact male pigs were artificially reared from postnatal day 2–30. The eyeblink conditioning setup consisted of a sound-damping box with a hammock that pigs were placed in, which allowed the pig to remain comfortable yet maintain a typical range of head motion. In a delay conditioning paradigm, the conditional stimulus (CS) was a 550 ms blue light-emitting diode (LED), the unconditional stimulus (US) was a 50 ms eye air-puff, the CS-US interval was 500 ms. Starting at postnatal day 14, pigs were habituated for 5 days to the eyeblink conditioning setup, followed by 5 daily sessions of acquisition training (40 paired CS-US trials each day).Results: The group-averaged amplitude of conditioned eyelid responses gradually increased over the course of the 5 days of training, indicating that pigs learned to make the association between the LED light CS and the air-puff US. A similar increase was found for the conditioned response (CR) probability: the group-averaged CR probability on session 1 was about 12% and reached a CR probability of 55% on day 5. The latency to CR peak time lacked a temporal preference in the first session but clearly showed preference from the moment that animals started to show more CRs in session 2 and onwards whereby the eyelid was maximally closed exactly at the moment that the US would be delivered.Conclusion: We concluded that 3-week-old pigs have the capability of performing in a cerebellar classical conditioning task, demonstrating for the first time that eyeblink conditioning in young pigs has the potential to be a valuable behavioral tool to measure neurodevelopment.


2021 ◽  
Author(s):  
Henk-Jan Boele ◽  
Sangyun Joung ◽  
Joanne Fil ◽  
Austin T Mudd ◽  
Stephen A Fleming ◽  
...  

Intro: Pigs have been an increasingly popular preclinical model in nutritional neuroscience, as their anatomy, physiology, and nutrition requirements are highly comparable to those of humans. Eyeblink conditioning is one of the most well-validated behavioral paradigms in neuroscience to study underlying mechanisms of learning and memory formation in the cerebellum. Eyeblink conditioning has been performed in many species but has never been done on young pigs. Therefore, our aim here was to develop and validate an eyeblink conditioning paradigm in young pigs. Method: Eighteen intact male pigs were artificially reared from postnatal day 2 to 30. The eyeblink conditioning setup consisted of a sound-damping box with a hammock that pigs were placed in, which allowed the pig to remain comfortable yet maintain a typical range of head motion. In a delay conditioning paradigm, the conditional stimulus (CS) was a 550 ms blue light-emitting diode (LED), the unconditional stimulus (US) was a 50 ms eye air-puff, the CS-US interval was 500 ms. Starting at postnatal day 14, pigs were habituated for five days to the eyeblink conditioning setup, followed by 5 daily sessions of acquisition training (40 paired CS-US trials each day). Results: The group-averaged amplitude of eyelid responses gradually increased over the course of the five days of training, indicating that pigs learned to make the association between the LED light CS and the air-puff US. A similar increase was found for the conditioned response (CR) probability: the group-averaged CR probability on session 1 was about 12% and reached a CR probability of 55% on day 5. The latency to CR peak time lacked a temporal preference in the first session, but clearly showed preference from the moment that animals started to show more CRs in session 2 and onwards whereby the eyelid was maximally closed exactly at the moment that the US would be delivered. Conclusion: We concluded that 4-week-old pigs have the capability of performing in a cerebellar classical association learning task, demonstrating for the first time that eyeblink conditioning in young pigs has the potential to be a valuable behavioral tool to measure neurodevelopment.


Energies ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 961
Author(s):  
Gabriel Constantino de Lima ◽  
Andre Luiz Lopes Toledo ◽  
Leonidas Bourikas

The energy sector and electricity generation in particular, is responsible for a great share of the global greenhouse gas (GHG) emissions. World electricity generation is still largely based on the burning of fossil fuels. However, Brazil has already a very low electricity carbon intensity due to the country’s large hydropower capacity. In countries with low grid carbon intensities such as Brazil, the investment in photovoltaic solar systems (PVSS) even if it is cost-effective, might become challenging as any new generation competes essentially against other renewable generation and the carbon offset is not a key driver for investment anymore. This study builds further upon that case to examine if national renewable energy incentives could actually lead to an increase of global net carbon emissions from the installation of PVSS in countries with a low grid carbon intensity. The study presents a life cycle analysis (LCA) of ten photovoltaic systems representative of the different operational conditions in regions across Brazil. It was found that the average energy payback time of the studied PV plants is between 3 and 5 years of operation. This result shows the feasibility and viability of such investments in the Brazilian context. When the LCA was integrated into the analysis though, the results showed that the “local” direct emissions avoidance from two out of ten studied PV plants would not manage to offset their “global” life cycle emissions due to the 2020 projected Brazilian grid emission factor which is already low. It is important to recognize that public policies of unrestricted, unconditional stimulus to photovoltaic systems investment might not help towards reducing global net emissions when the PV systems are installed at countries with a low carbon emission electric matrix. That is also something to consider for other countries as the carbon intensity of their grids will start reducing at levels similar to Brazil’s. It is likely that in the near future, the real net carbon offset achieved by PV systems at the global level will be largely defined by the manufacture procedures and the production’s carbon intensity at the country of origin of the PV panels.


2021 ◽  
Author(s):  
Jinxia Wang ◽  
Xiaoying Sun ◽  
Jiachen Lu ◽  
Haoran Dou ◽  
Yi Lei

Abstract A major limitation of fear generalization research entails the confusing unconditional stimulus—it can often induce not only fear but also disgust. Differences between the two threat-related emotions during conditioning and generalization are currently unknown. To address this issue, 32 college students completed threat conditioning tasks including conditioned stimuli paired with fear or disgust images. A block design was used to divide fear and disgust into two randomly ordered blocks, enabling examination of differences between fear and disgust by recording subjective expectations and eye movement in the generalization process. The results revealed that participants reported larger subjective expectations of fear-related GS1 (generalized stimuli) and GS2 than disgust-related GS1 and GS2, and fear led to longer reaction times than disgust in both conditioning and generalization phases. The pupil size and fixation duration for fear stimuli were larger than for disgust stimuli, suggesting that fear generalization has a steeper gradient than disgust generalization. Participants paid more attention to fear and were more inclined to avoid disgust stimuli. These findings provide new, albeit preliminary, evidence of the differences between fear and disgust stimuli in generalization, and may offer insight into the treatment of clinical anxiety and other fear- or disgust-related disorders.


2020 ◽  
Vol 14 ◽  
Author(s):  
Justin D. Handy ◽  
W. Geoffrey Wright ◽  
Amanda Haskell ◽  
Labeeby Servatius ◽  
Richard J. Servatius

Enhanced acquisition of eyeblink conditioning is observed in active duty military and veterans expressing PTSD symptoms (PTSD+) and those expressing temperamental vulnerabilities to develop PTSD after traumatic experiences, such as behaviorally inhibited temperament. There is a growing literature showing persistent cerebellar abnormalities in those experiencing mild traumatic brain injury (mTBI+) as well as linkages between mTBI and PTSD. With the dependency of eyeblink conditioning on cerebellar processes, the impact of mTBI on eyeblink conditioning in veterans expressing PTSD is unknown. The present study assessed eyeblink conditioning in veterans during two sessions separated by 1 week. With a focus on the accelerated learning of veterans expressing PTSD, training utilized a protocol which degrades learning through interspersing conditioned stimulus (CS) exposures amongst delay-type trials of CS and unconditional stimulus (US) co-terminating trials. Faster acquisition of the eyeblink conditioned responses (CR) was observed in PTSD during Week 1. The Week 2 assessment revealed an interaction of mTBI and PTSD, such that asymptotic performance of PTSD+ was greater than PTSD− among mTBI− veterans, whereas these groups did not differ in mTBI+ veterans. To further examine the relationship between enhanced sensitivity to acquire eyeblink conditioning and PTSD, cluster analysis was performed based on performance across training sessions. Those with enhanced sensitivity to acquire eyeblink conditioned responses expressed more PTSD symptoms, which were specific to Cluster C symptoms of avoidance, in addition to greater behavioral inhibition. These results support the continued investigation of the conditioned eyeblink response as a behavioral indicator of stress-related psychopathology.


2020 ◽  
Vol 287 (1938) ◽  
pp. 20201234
Author(s):  
Matthias Durrieu ◽  
Antoine Wystrach ◽  
Patrick Arrufat ◽  
Martin Giurfa ◽  
Guillaume Isabel

Associative learning allows animals to establish links between stimuli based on their concomitance. In the case of Pavlovian conditioning, a single stimulus A (the conditional stimulus, CS) is reinforced unambiguously with an unconditional stimulus (US) eliciting an innate response. This conditioning constitutes an ‘elemental’ association to elicit a learnt response from A + without US presentation after learning. However, associative learning may involve a ‘complex’ CS composed of several components. In that case, the compound may predict a different outcome than the components taken separately, leading to ambiguity and requiring the animal to perform so-called non-elemental discrimination. Here, we focus on such a non-elemental task, the negative patterning (NP) problem, and provide the first evidence of NP solving in Drosophila . We show that Drosophila learn to discriminate a simple component (A or B) associated with electric shocks (+) from an odour mixture composed either partly (called ‘feature-negative discrimination’ A + versus AB − ) or entirely (called ‘NP’ A + B + versus AB − ) of the shock-associated components. Furthermore, we show that conditioning repetition results in a transition from an elemental to a configural representation of the mixture required to solve the NP task, highlighting the cognitive flexibility of Drosophila .


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