Abstract
A non-enzymatic glucose detection electrode that can operate in a neutral environment is a key technology for continuous glucose sensing. In this study, semiconductor micro-electromechanical manufacturing processes, precision micro-molding, hot embossing, and electrochemical deposition are integrated to fabricate Pt/Au nano-alloy electrodes for the enzyme-free detection of glucose in a neutral environment. The sensitivity of the proposed neutral enzyme-free glucose sensing scheme is measured to be 2.82 µA mM− 1 cm− 2 with a linear range of 1.39–13.9 mM and a detection limit of 0.482 mM. Further investigations in human serum confirmed the feasibility of the proposed Pt/Au nano alloy electrode in clinical application. This novel glucose biosensor possesses the advantages of technology foresight, good detection performance, and high feasibility of mass production.