Neutral Non-Enzymatic Glucose Biosensors Based on an Electrochemically Deposited Pt/Au Nano-Alloy Electrode

Abstract A non-enzymatic glucose detection electrode that can operate in a neutral environment is a key technology for continuous glucose sensing. In this study, semiconductor micro-electromechanical manufacturing processes, precision micro-molding, hot embossing, and electrochemical deposition are integrated to fabricate Pt/Au nano-alloy electrodes for the enzyme-free detection of glucose in a neutral environment. The sensitivity of the proposed neutral enzyme-free glucose sensing scheme is measured to be 2.82 µA mM− 1 cm− 2 with a linear range of 1.39–13.9 mM and a detection limit of 0.482 mM. Further investigations in human serum confirmed the feasibility of the proposed Pt/Au nano alloy electrode in clinical application. This novel glucose biosensor possesses the advantages of technology foresight, good detection performance, and high feasibility of mass production.

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Preparation of an Amperometric Glucose Biosensor on Polyaniline-Coated Graphite

Journal of Sensors ◽

10.1155/2021/8832748 ◽

2021 ◽

Vol 2021 ◽

pp. 1-7

Author(s):

Shova Neupane ◽

Suresh Bhusal ◽

Vivek Subedi ◽

Krishna Badan Nakarmi ◽

Dipak Kumar Gupta ◽

...

Keyword(s):

Photoelectron Spectroscopy ◽

Glucose Concentration ◽

Reduction Potential ◽

Low Cost ◽

Glucose Sensing ◽

Glucose Biosensor ◽

Pani Film ◽

Current Response ◽

Electrochemically Deposited ◽

Effects Of Ph

Control of glucose concentration has tremendous significance in medical diagnosis, pharmaceuticals, food, and fermentation industries. Herein, we report on the fabrication of a facile, low-cost, and sensitive enzyme-based amperometric sensor using the electrochemically deposited polyaniline (PANI) film on a graphite electrode. PANI was deposited from an aqueous solution of 0.2 M aniline in 1.0 M hydrocholoric acid (HCl) by cyclic voltammetry (CV). Surface morphology and composition characterization of the PANI film were carried out by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and Fourier-transform infrared (FTIR) spectroscopy. Potentiostatic immobilization of glucose oxidase (GOX) enzyme in the PANI film was carried out at 0.75 V to fabricate an amperometric glucose biosensor (GOx/PANI/graphite biosensor). The glucose concentration response of the prepared sensor was studied amperometrically by detecting hydrogen peroxide (H2O2). The detection of H2O2 was optimized by calibrating the effects of pH, reduction potential, and background current. A reduction potential of -0.4 V at pH 6 was the best combination to get a maximum amperometric response of the GOx/PANI/graphite biosensor. A stable current response was obtained in 4 min with a high reproducibility in linearity within the concentration range of 0.01 M-0.1 M D-glucose. Therefore, the fabricated GOx/PANI/graphite biosensor could be a promising candidate for glucose sensing.

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Gold Nanoparticle-Polyaniline Composite Films for Glucose Sensing

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2008.18322 ◽

2008 ◽

Vol 8 (6) ◽

pp. 3158-3163 ◽

Cited By ~ 1

Author(s):

Pratibha Pandey ◽

S. P. Singh ◽

Sunil K. Arya ◽

Anju Sharma ◽

Monika Datta ◽

...

Keyword(s):

Gold Nanoparticles ◽

Indium Tin Oxide ◽

Composite Films ◽

Glucose Sensing ◽

Glucose Biosensor ◽

Covalent Immobilization ◽

Amperometric Response ◽

Electrochemically Deposited ◽

Response Current ◽

Menten Constant

Gold nanoparticles (AuNPs) have been self-assembled onto electrochemically deposited polyaniline (PANI) films on indium-tin-oxide (ITO) coated glass plates to fabricate glucose biosensor. The covalent immobilization of glucose oxidase (GOx) in the near vicinity of gold nanoparticles has been obtained using N-ethyl-N′-(3-dimethylaminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS), chemistry between amino groups of PANI and COOH groups of GOx. These AuNPs-PANI/ITO and GOx/AuNPs-PANI/ITO composite films have been characterized using Fourier transform infra red (FTIR) and cyclic voltammetry (CV) techniques, respectively. The fast electron transfer from the modified PANI surface to electrode is indicated by the observed increase in amperometric response current of these GOx/AuNPs-PANI/ITO bioelectrodes. These GOx/AuNPs-PANI/ITO bioelectrodes exhibit response time of 10 s, linearity from 50 to 300 mg/dl and show value of apparent Michaelis-Menten constant (Kmapp) of 2.2 mM.

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