Isovitexin Inhibits Ginkgolic Acids-Induced Inflammation Through Downregulating SHP2 Activation

It has been reported that Celtis sinensis Pers. is employed as a folk medicine for the treatment of inflammatory diseases. But the mechanism supporting its use as anti-inflammatory remains unclear. To investigate the anti-inflammatory of Celtis sinensis Pers. ICR mice were provided Celtis sinensis Pers. leaf extract (CLE) at 100, 200 mg/kg after ginkgolic acids (GA) sensitization. Our data showed that CLE and the main flavonoid isovitexin in CLE could ameliorate GA-induced contact dermatitis in mice. Ear swelling, inflammatory cell infiltration and splenomegaly were inhibited significantly by isovitexin, while the weight loss of mice in the isovitexin-treated group was much better than that in the dexamethasone-treated group (positive control drug). It has been reported in previous research that GA-induced inflammation is closely related to the T cell response. Therefore, T cells were the focus of the anti-inflammatory effect of isovitexin in this paper. The in vivo results showed that isovitexin (10, 20 mg/kg) inhibited the expression of proinflammatory cytokines (TNF-α, IFN-γ, IL-2 and IL-17A) in lymph nodes, inhibited the secretion of cytokines into the serum from mice with contact dermatitis and promoted the expression of apoptosis-related proteins. In vitro, isovitexin also induced apoptosis and inhibited proinflammatory cytokine expression in Con A-activated T cells. Further study showed that the MAPK and STAT signaling pathways and the phosphorylation of SHP2 were inhibited by isovitexin. Both molecular docking and biological experiments indicated that SHP2 may be an anti-inflammatory target of isovitexin in T cells. Taken together, isovitexin can serve as a potential natural agent for the treatment or prevention of GA-induced inflammatory problems.

Isovitexin, a main Flavonoid Compound from Celtis Sinensis Pers. Leaves, Exerts Activity Against Inflammation caused by Contacting Ginkgolic Acids in Ginkgo Fruit through Downregulating SHP2 Activation

It has been reported that Celtis sinensis Pers. is employed as a folk medicine for the treatment of inflammation diseases. But the mechanism supporting its use as anti-inflammatory remain unclear. To investigate the anti-inflammatory of Celtis sinensis Pers. ICR mice were provided Celtis sinensis Pers. leaf extract (CLE) at 100, 200mg/kg after ginkgolic acids (GA) sensitization. Our data showed that CLE and the main flavonoid isovitexin in CLE could ameliorate GA-induced contact dermatitis in mice. Ear swelling, inflammatory cell infiltration and splenomegaly were inhibited significantly by isovitexin, while the weight loss of mice in the isovitexin-treated group was much better than that in the dexamethasone-treated group (positive control drug). It has been reported in previous research that GA-induced inflammation is closely related to the T cell response. Therefore, T cells were the focus of the anti-inflammatory effect of isovitexin in this paper. The in vivo results showed that isovitexin (10, 20mg/kg) inhibited the expression of proinflammatory cytokines (TNF-α, IFN-γ, IL-2 and IL-17A) in lymph nodes, inhibited the secretion of cytokines into the serum from mice with contact dermatitis and promoted the expression of apoptosis-related proteins. In vitro, isovitexin also induced apoptosis and proinflammatory cytokine expression in Con A-activated T cells. Further study showed that the MAPK and STAT signaling pathways and the phosphorylation of SHP2 were inhibited by isovitexin. Both molecular docking and biological experiments indicated that SHP2 may be an anti-inflammatory target of isovitexin in T cells. Taken together, isovitexin can serve as a potential natural agent for the treatment or prevention of GA-induced inflammatory problems.

Efficient extraction of bioactive flavonoids from Celtis sinensis leaves using deep eutectic solvent as green media

In recent years, deep eutectic solvent (DES) has attracted comprehensive attention on the extraction of natural products, and is regarded as an alternative to traditional organic solvents for the environmental advantages.

Screening of broad-leaved tree species to restore Eucalyptus urophylla Blake forest on Sanjiao Island, China

This study aimed to test the allelopathic effects of Eucalytus urophylla Blake. forest soil on 5-native broad-leaved tree species (Bauhinia purpurea L., Acacia confusa Merr., Acacia podalyriifolia A. Cunn. ex G. Don., Celtis sinensis Pers. Liquidambar formosana Hance) to select suitable species for restoration of E. urophylla forest. Three types of soils [E. urophylla soil, other plants soil and bare soil] were used to determine their allelopathic potential. The total phenols concentrations in soil were measured, and the correlations between total phenols and plant growth indices were analyzed. E. urophylla soil significantly inhibited the basal diameters of Bauhinia purpurea L. and Acacia confusa Merr. and biomass of Acacia podalyriifolia A. Cunn. ex G. Don. but significantly improved the biomass of Celtis sinensis Pers. and diameter of Liquidambar formosana Hance. The total phenols in soil accounted for these results, except for A. podalyriifolia. Thus, L. formosana, C. sinensis and A. confusa can be suggested as suitable tree spp to establish mixed stands with E. urophylla. Besides, B. purpurea, A. podalyriifolia, A. confusa and L. formosana can be used for restoration in bare soil, while B. purpurea, A. confusa and L. formosana are suitable species to restore vegetation in other plants soil. Keywords: Acacia confuse, Acacia podalyriifolia, Allelopathy, bare soils, Bauhinia purpurea, broad-leaved trees, Celtis sinensis, Eucalytus urophylla, E. urophylla soil, forest restoration, island, other plants soil, rhizosphere soil, total phenols

Adatok és kiegészítések a magyarországi adventív flóra kivadult, meghonosodott és potenciális inváziós fajainak ismeretéhez

47 dunántúli és alföldi település flórájának felmérése kapcsán, 52 magyarországi flóratérképezési kvadrátból, összesen 157 hazánkban adventív növényfaj adatait közöljük. Az itt sorolt ritka, érdekes vagy a legutóbbi hazai neofiton fajokat összegző listán nem szereplő növényeken belül 65 az ország flórájára új taxonnak számít. A listázott fajok egy része ritka, alkalmilag elvaduló (pl. Lagurus ovatus, Salvia coccinea), az ország jelenlegi klimatikus viszonyai között szaporodni nem képes (pl. Cupressus sempervirens, Punica granatum) faj, de akadnak közöttük potenciális özönnövények is (pl. Celtis sinensis, Lonicera japonica). A kitekintésben felhívjuk a figyelmet az urbánus területek idegenhonos növényfajainak megjelenését és terjeszkedését előidéző gyakorlatokra (pl. zöldterület fenntartás), az e tekintetben speciális urbánus élőhelyekre (pl. magán és botanikus kertek), továbbá a jövőben a klímaváltozás hatására egyre gyakrabban fellépő folyamatok jelentőségére (pl. laurifillizáció, termofil taxonok terjeszkedése).