An Overview in Green Chromatographic Analysis from Pharmaceutical Preparations

2020 ◽  
Vol 16 ◽  
Author(s):  
Ayşegül Doğan ◽  
Cemil Can Eylem ◽  
Nursabah E. Başcı Akduman
Keyword(s):  
Liquid Chromatography ◽  
Green Chemistry ◽  
Flow Rate ◽  
Pharmaceutical Analysis ◽  
Chromatographic Analysis ◽  
Pharmaceutical Preparations ◽  
Sample Collection ◽  
Green Approach ◽  
Waste Production ◽  
Green Chromatography

Background: Green Chemistry has emerged as a new field of study in chemistry in the past 15 years and is intended to be sustainable at the molecular level. Green Chemistry has focused on harmless chemical technologies to meet both environmental and targets. The purpose of Green Chromatography is to protect the environment and the analyst like Green Chemisty. Objective: The main target is to reduce the consumption of hazardous solvent and to replacement of toxic and environmentally hazardous solvents with more benign alternatives. Methods: In addition, reducing the flow rate (decrease column interdiameter etc.) in order to miniaturizing the waste generation is also within the field of green chromatography. On the other hand, the most effective technique for the “Green” approach can be miniaturized completely. Flow rate decreases from mL to microliter per minute. Thus, waste production and solvent consumption are significantly reduced. In recent studies, studies without organic solvents such as SFC, SBWC are frequently encountered. Results: Chromatographic analysis has the potential to become greener at all stages from sample collection and preparation to separation and final determination. Liquid chromatography is often used in pharmaceutical analyzes. Tons of wastes are produced every year for pharmaceutical analysis. For this reason, greening approach in liquid chromatography becomes very important for human and environmental health in pharmaceutical analysis. Conclusion: This review discusses the approaches used to achieve the goals of green chromatography, aimed at protecting the environment and the analyst in pharmaceutical analysis. While one cannot completely convert the analysis to the green, the steps taken to green are very valuable particularly in widerange used HPLC both in drug quality control laboratories in industry and in reaserch studies.

Exploitation of flow-based procedures for reagentless hydrochlorothiazide determination and accelerated degradation studies of pharmaceutical preparations

2021 ◽  
Author(s):  
Vivian Maringolo ◽  
Fernanda Caroline Aleixo ◽  
Alexandre Carvalho ◽  
Diogo Librandi Rocha
Keyword(s):  
High Performance Liquid Chromatography ◽  
Patient Safety ◽  
Liquid Chromatography ◽  
Treatment Efficacy ◽  
Pharmaceutical Analysis ◽  
High Performance ◽  
Stress Testing ◽  
Pharmaceutical Preparations ◽  
Standard Technique ◽  
Accelerated Degradation

Drug quality assessment and stress testing are important to ensure both treatment efficacy and patient safety. High performance liquid chromatography may be considered a standard technique for pharmaceutical analysis, showing...

scholarly journals HPLC ASSESSMENT OF VIOLAXANTHIN CYCLE’S FUNCTION UNDER EXCESSIVE LIGHT IN MOUGEOTIA SP. ALGAE

1970 ◽  
Vol 63 ◽  
Author(s):  
E. Muntean ◽  
V. Bercea ◽  
N. Dragoş ◽  
Nicoleta Muntean
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Flow Rate ◽  
Chromatographic Analysis ◽  
Mobile Phase ◽  
High Performance ◽  
Carotenoid Biosynthesis ◽  
Biological Research ◽  
Protective Function ◽  
Mobile Phases

The protective function of xanthophyll cycle was investigated in the green algae Mougeotia sp. Agardt (AICB 560) originating from the collection belonging to Institute of Biological Research Cluj Napoca, using excessive light. High performance liquid chromatography was used to analyze carotenoids involved in violaxanthin cycle in this matrix. Separations were performed on an Agilent 1100 system, using a Nucleosil 120-5 C18 column and the following mobile phases: A - acetonitrile : water (9 : 1) and B - ethyl acetate. The flow rate of the mobile phase was 1 ml/ min. and the solvent gradient was as follows: from 0 to 20 min. - 10% to 70% B, then from 20 to 30 min. - 70% to 10% B. Chromatographic analysis demonstrated a particular behavior of AICB 560 under intense illumination: the major de-epoxidation product is not zeaxanthin, but antheraxanthin. More than that, the illumination conditions affect the whole carotenoid biosynthesis, starting with the violaxanthin cycle’s pigments precursor: b-carotene.

Simultaneous Determination of Methocarbamol and Paracetamol in the Presence of Three Related Substances by Ultra Performance Liquid Chromatography

2019 ◽  
Vol 15 (5) ◽  
pp. 505-510
Author(s):  
Yanjuan Zheng ◽  
Qiushi Peng ◽  
Rui Dong ◽  
Tingyu Chen ◽  
Yi Bao ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Pharmaceutical Preparations ◽  
Gradient Elution ◽  
Ultra Performance Liquid Chromatography ◽  
Related Substances ◽  
Bulk Powder ◽  
Highly Sensitive ◽  
Optimal Protocol ◽  
Acquity Uplc

Introduction: A rapid, and accurate Ultra Performance Liquid Chromatography (UPLC) method was developed to simultaneously analyze Methocarbamol, Paracetamol and the related substances Materials and Methods: Waters ACQUITY UPLC® BEH Phenyl C18 column was used in conjunction with UV detection at 225nm. Gradient elution with 0.05M, pH 6 phosphate buffer and acetonitrile flow at 0.3mL /min rate were used to separate the substances. The retention times for 4-Aminopheno, Paracetamol, Guaifenesin, Methocarbamol, and 4-Chloroacetanilide were 1.319 minute, 2.224 minute, 4.467 minute, 4.769 minute and 5.433 minute respectively. The concentration was linear in the range of 2-100 µg/ml for Methocarbamol, and 1-100 µg/mL for Paracetamol. The percentage recoveries were between 99.28±1.23% to 100.57±0.99% for Methocarbamol, and between 99.08±1.23% to 101.23±1.39% for Paracetamol. Results and Discussion: The validated optimal protocol is robust and accurate for simultaneous analysis of Methocarbamol, Paracetamol and the related substances, applicable for bulk powder as well as pharmaceutical formulation. Conclusion: In this paper, a highly sensitive, accurate, and precise UPLC method with UV-Vis detection was developed and validated for quality control of MET and PAR in bulk as well as in pharmaceutical preparations.

Optimum HPLC Conditions for Determination of Dibucaine HCL, Fluocortolone Pivalate and Fluocortolone Caproate by Using Experimental Design

2018 ◽  
Vol 15 (1) ◽  
pp. 32-38 ◽  
Author(s):  
Bürge Aşçı ◽  
Mesut Koç
Keyword(s):  
Experimental Design ◽  
Flow Rate ◽  
Mobile Phase ◽  
High Performance ◽  
Limit Of Detection ◽  
Pharmaceutical Preparations ◽  
Acid Mixture ◽  
Solution Stability ◽  
Uv Detector

Introduction:This paper presents the development and validation of a novel, fast, sensitive and accurate high performance liquid chromatography (HPLC) method for the simultaneous quantitative determination of dibucaine HCl, fluocortolone pivalate and fluocortolone caproate in pharmaceutical preparations.Experiment:Development of the chromatographic method was based on an experimental design approach. A five-level-three-factor central composite design requiring 20 experiments in this optimization study was performed in order to evaluate the effects of three independent variances including mobile phase ratio, flow rate and amount of acid in the mobile phase.Conclusion:The optimum composition for mobile phase was found as a methanol:water:acetic acid mixture at 71.6 : 26.4 : 2 (v/v/v) ratio and optimum separation was acquired by isocratic elution with a flow rate of 1.3 mL/min. The analytes were detected using a UV detector at 240 nm. The developed method was validated in terms of linearity, precision, accuracy, limit of detection/quantitation and solution stability and successfully applied to the determination of dibucaine HCl, fluocortolone pivalate and fluocortolone caproate in pharmaceutical topical formulations such as suppositories and ointments.

4-Nitrophenol in 4-nitrophenyl phosphate, a substrate for alkaline phosphatase, as measured by paired-ion high-performance liquid chromatography.

1977 ◽  
Vol 23 (12) ◽  
pp. 2288-2291 ◽  
Author(s):  
P H Culbreth ◽  
I W Duncan ◽  
C A Burtis
Keyword(s):  
Alkaline Phosphatase ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Flow Rate ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Nitrophenyl Phosphate ◽  
Phase Column ◽  
Reversed Phase Column

Abstract We used paired-ion high-performance liquid chromatography to determine the 4-nitrophenol content of 4-nitrophenyl phosphate, a substrate for alkaline phosphatase analysis. This was done on a reversed-phase column with a mobile phase of methanol/water, 45/55 by vol, containing 3 ml of tetrabutylammonium phosphate reagent per 200 ml of solvent. At a flow rate of 1 ml/min, 4-nitrophenol was eluted at 9 min and monitored at 404 nm; 4-nitrophenyl phosphate was eluted at 5 min and could be monitored at 311 nm. Samples of 4-nitrophenyl phosphate obtained from several sources contained 0.3 to 7.8 mole of 4-nitrophenol per mole of 4-nitrophenyl phosphate.

Quantitative aspects of flow-rate in liquid chromatography and reaction-detection liquid chromatography

1983 ◽  
Vol 17 (1) ◽  
pp. 4-8 ◽  
Author(s):  
J. H. M. van den Berg ◽  
C. B. M. Didden ◽  
R. S. Deelder
Keyword(s):  
Liquid Chromatography ◽  
Flow Rate
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