RESEARCH OF AMYLOLYTIC ACTIVITY OF CULTURAL LIQUIDS IN BIOTECHNOLOGY OF SYMBIOTIC CROPS МЕDUSOMYCES GISEVII AND ORYZAMYCES INDICI

Based on the literature review found that the natural symbionts Medusomyces gisevii and Oryzamyces indici biotechnology are a valuable objects. It is urgent to find ways to impact on it in order to obtain these or other products of its life activity. At present, it is urgent to search for microorganisms producing enzymes, including amylase. One of the most promising in terms of biological objects is a natural microbial symbiont Medusomyces Gisevii (tea fungus) and Oryzamyces Indici, which, thanks to the not identical, microbiological composition and different growing conditions may have a different composition of metabolites. Studies of the amylolytic activity of the culture liquid Medusomyces Gisevii and Oryzamyces indici with different cultivation periods have been carried out. Cultivation of the fungus was carried out in the laboratory according to the classic method. The optimal concentration of sucrose for Medusomyces Gisevii and Oryzamyces Indici biomass growth was set at 5%. Sucrose concentration of 15% and above is not recommended for use due to inhibition of biomass growth with increasing concentration of carbohydrates in the culture medium. It is established that these symbionts start to show the amylolytic activity already on the 10th day of cultivation cultivation in standard nutrient medium, medium supplemented with 10 % milk and serum-based medium and stores it in the course of the experiment (30 days). Over time the amylolytic activity increases. However, the intensity of metabolism of the microorganisms, the criterion of which is the ratio of the total and exogenous amylase is most pronounced in the early stages of cultivation. It was found that the culture medium of polycultures shows high amylolytic activity. This fact allows us to consider the Medusomyces Gisevii and Oryzamyces Indici inoculum as a promising biotechnological raw material source of amylase enzyme.

PHYSICO-CHEMICAL CHARACTERISTICS OF RUSSIAN TEA FUNGUS: KOMBUCHA

Objective: To characterize various physicochemical parameters for Russian tea fungus: Kombucha. Methods: In the present investigation, various physicochemical analyses of kombucha such as, estimation of biomass, ascorbic acid, acetic acid, carotenoid contents, antioxidant activity and antibacterial activity against E. coli, Salmonella sp and Staphylococcus aureus were carried out after growing at different temperature and pH with varied concentrations of 5, 10, 15 and 20% tea powder boiled for 10 min along with 5% sugar and filtered to obtain tea decoction. Results: The bacteria in kombucha were Gram-negative, flexible rods. Both bacteria and yeast were positive for acid and gas production with glucose and sucrose and negative with lactose. A highest biomass content of 33.39 and 32.9 g/l in 5 and 10% tea decoction respectively was observed when grown statically at 25 °C and pH 5.0. Highest carotenoids (92.5 μg/ml) content was found at normal pH without setting, while 85 and 84 μg/ml at pH 4.5 and 5.0 respectively under static condition. A highest DPPH(2,2-diphenyl-1-picrylhydrazyl) inhibition of 76.95% with IC50 value 3.26 mg/ml and ascorbic acid content of 33.19% were found with 5% tea at 25 °C and pH 4.5. Acetic acid, which was shown to be one of the antimicrobial agents in kombucha had the highest strength of 2.25 g/l in 5% tea grown at 25 °C and normal pH, while 4.5g/l at pH 4.5. The antibacterial activity showed the sensitivity of Escherichia Coli with 57% and Salmonella sp with 42.9% inhibition for kombucha grown at 25 °C and pH 4.5 when compared to positive control.Conclusion: Kombucha, a miracle drink can be exploited furthermore for human well-being through combating various health issues due to changing lifestyle.